FastQCFastQC Report
Sat 31 Dec 2022
SRR5849369.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849369.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74006
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGCCGTCT4860.6567035105261736No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGC3570.4823933194605843No Hit
GTGTTTGGGATTCTACCATAATATAAAGAAAAAAAGGAAACAGTGCTTGCTC870.1175580358349323No Hit
ACGCTCGGGATGAGATGTTAGCACTGGGATAGGCTGAGAGAAAGGTACACAG860.11620679404372618No Hit
ATCTAGGGGCTTCTTTTATGAAGCTCAGTTTGGCAGAATATAGAATTGTTGA850.11485555225252007No Hit
TTAGTGGGGAAGACAGTACAAAAACACTGGCACTTGAATGTTGAATGTCACC830.11215306867010782No Hit
ACTTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC830.11215306867010782No Hit
TGCTTAGGGATTACAGTTGCCTTTCACATACTCAGAAAGTTAAGTAGAGACA820.11080182687890172No Hit
AAACCAGGGAGTGGAGGCATTGATCGTGAATTTTGAACACAGAAAACCTCAG810.10945058508769559No Hit
CCGCCCGGGGGCCCACGTGGCCTCCGCGGGCCCCGCCAGAGCCTGCGCCCGG810.10945058508769559No Hit
TAATGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC810.10945058508769559No Hit
AAAGTTGGGAGACATGCAAATGGCCAACAGATATGTGAAAAACAAATGCTCA780.10539685971407724No Hit
AACTTTGGGCATCGTTGATAAAAATGCAAAGTCAAATAAAAGGTGTCTTGGT770.10404561792287112No Hit
GCATATGGGGAAGGCAGCATGACTGTTTGTACTACAAAGAAGACTTTTGATC770.10404561792287112No Hit
TCAAGCGGGGCATATTTAATTTTTGGAATCTTTGCAATATCATGAAAAGTTG760.10269437613166499No Hit
CAACCAGGGAATTGTCTTTTTCTTTAAGAGTTAAGAGACGATCACACTGGTT750.10134313434045888No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)