FastQCFastQC Report
Sat 31 Dec 2022
SRR5849344.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849344.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132685
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGC10230.7709989825526623No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGCCGTCT5660.42657421713079857No Hit
AGCTTAGGGAACATCTTTTTGTCTCCCAGCAGACTGACCCAGTGTCTTGTAC1960.14771828013716698No Hit
AAATAAGGGCTAGCCAAATCCGGCCACAAGCGTCTCATCAGGGATCTCCAGC1840.13867430380223839No Hit
GCTTTGGGCAGATATGGAAAAATCACACTTCACAAGAGATCCATCCCAGCTT1830.137920639107661No Hit
CGTGACGGGAAGAGCAATGTTTTGCTTTGCTCTCTGGTCCATGAACTCATTT1770.1333986509401967No Hit
TAATAAGGGATTTATCTACATGTCTTTAGCATCTAGCAAATAATAGATGCTC1710.1288766627727324No Hit
TATTCAGGGAGAGAAAGAAATAGTTCTATAGATAATTTCCCTAAGTAGCTTT1670.12586200399442288No Hit
GCACGGGACAGAAATGATGTTGCAGTTATGGTTATAGCACAAGGCAATTGCC1660.1251083392998455No Hit
AGCTAAGGGTGGAGAAATATTTAAAATTAGGTGGTTTTTTTCAAGCACGAGT1650.12435467460526811No Hit
CAACCCAGGGATAAAATAATAGTGCTTTTGCAACTTACTGCATCTAAAATTT1650.12435467460526811No Hit
TTATGTGGGAACTCAACGAGTCCTAACTTGGCAGCATGTATAATCATCGTTG1650.12435467460526811No Hit
TTCATGGGGATAAAGACTCAATTCAGTATCACCTCGTGGTTTTATCTCATAG1650.12435467460526811No Hit
ACGCTGGGGCACAATTTTACTTATGTGCTTACGGACATCCAATAGTTTCATC1650.12435467460526811No Hit
AACTATGGGCAGCTGTAGCTGTTTGTTGAGGGTGTCCTATGTGCTAGGTGTG1640.12360100991069074No Hit
TAGTCAGGGGACTTCTAGGTACTAAGAGTCAAGGAGGCAGGACTACATTTTC1610.1213400158269586No Hit
GGCTTTGGGATGGATAACAGCAAGTGTTGGCAAGGATATGGAGAAATTGGAG1580.11907902174322645No Hit
TTTGTCCGGGTATAGGGAGAATAATTTAGATACACATGCTCAGAGCTGAGAT1570.11832535704864905No Hit
GACTTAGGGCGGGGCTGTGATTCTTCCCCTCTTGGGGCTGCTGCTCTCCCTC1560.11757169235407168No Hit
CTTATACGGGGGGTGAAAGAAGGTTAGGATGTAGAAAAGGGATTTAGAGACA1550.1168180276594943No Hit
TGTCTTGGGAGTGACCCAACATTTCAGCAAGGAGGTACTCATTGGGAAAAAT1530.11531069827033953No Hit
TAGCAAGGGGGTCTTCACTGACTGCACTTCTTCAGGAGAAGCTTTTGTTATC1530.11531069827033953No Hit
GCTGTTGGGGTACATTGGTAATTTAAACATCCAATAACAGGAAAATCTAGAT1520.11455703357576213No Hit
ATAGCGGGGACTGGCCGGGAGGAGCTAGAGGCGGGCGTCTGCACAGCGTCGC1520.11455703357576213No Hit
TCTGTGGGATTACTCATTGCTACGGCAGTTCAAAATGACTTGGAAAAAAACA1510.11380336888118475No Hit
CTTGAGGGGCAGAAACCAAACCAGAACTGACATACATACCAGAATTGGCACA1500.11304970418660738No Hit
AGTCTTGGGAATAACATAAAGCAAGTGAGCGACCTTATCTCTGTGCTTCGTG1490.11229603949202999No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCCTGTCTCT1490.11229603949202999No Hit
GTCTCACAAAAAAGAAAAGAAAACAAGAAAAAGAAAAATTGTGTATTGAATT1470.11078871010287523No Hit
AAAATAGGGGATACTGTTTTAAATAATCTGTAATTTCAATTTTTTTTTTTTT1470.11078871010287523No Hit
GTGCCAGGGAAAAATGTACACGTTTTTTCTTATTGTTACCACATGTTCACCT1460.11003504540829784No Hit
AATTTAGGGACAGGCAGCTGCACACGTTACAGAAGCTACGCATGGATCTGAT1460.11003504540829784No Hit
CTTGCCATGCGGAGGGGATATTCAAGAGAAGCTTATGTGGAGTTAGTTCACC1460.11003504540829784No Hit
ATTAAAGGGATCTCACAATTATATTAGCAGTGGTAACATGGGAAGTGAAGGG1440.10852771601914309No Hit
AAATATGGGGCAGATATGAGGTCTTAGACTGGGAACTGTCCCACACAAGAGC1440.10852771601914309No Hit
CGACATGGGTTATTTAAGGAAAAATAGATTCTTAAATACATGATATATAGGT1430.10777405132456569No Hit
CTACATTGGGGATTACCATTTCCTTACAAAAAGAAATTGATAATTGAAATTA1430.10777405132456569No Hit
TTTGCGGGGGCGGTTAGATCGCAAGGTCTACTAAGCTTTGGCCAGACTGTCT1430.10777405132456569No Hit
CGAAACGGGGTAGTTTGGATACAAAGCTGATTAAATTGGATCAAGAAATATT1420.10702038662998832No Hit
TTCTGTGGGGAACACCAAAATGAGAATTAGGATTGATTCTGATTCCAACTTT1410.10626672193541092No Hit
CGAACTGGGGTAGAAAACAGATAAGTGGCTTAGGCCAGTAGCAGGGGTAAGG1410.10626672193541092No Hit
TTTACTTAATAAACTGAAAAATTTTTAGGCAGTTTATATTCCTGTTGACTAG1400.10551305724083357No Hit
CAACTTGGGCAGGGTTTGTTCTCCTAACCACACCACCACACTGCCTGTAGAT1400.10551305724083357No Hit
ATAACAGGGAGCCATTGGCTTTATGACTCTGGAAAGGTGCCTGGCACATGGA1400.10551305724083357No Hit
CAGGACGGGAGAGGTAAACAAATGTATTTTGGCTTCATTAAACATTTTGATA1390.10475939254625617No Hit
CAGTTTGGGGAAAATCAGCAGAATTTCTTAATACTTCGCTGGTTCCTTCTTG1390.10475939254625617No Hit
TCCAATGGGATGTATGGAGGCAAAAACATTAGTTCACACACAAACATTGTTT1380.10400572785167878No Hit
TGCTAAGGGAGAAATGAAAGTGGTACATACTTTTTAAGGGAGGAGAACAGAA1370.1032520631571014No Hit
AAACTAGGGGTTGAAATGTGCTTAATTGGGTCTAGTAATACTCCACATTTTT1370.1032520631571014No Hit
TCAGCAGGGATAGATGCCAGAAAAGAAGGTGCCGCTATAAAATGCTTTCTTG1370.1032520631571014No Hit
TGATGCAGGGGCAGGAAAGGAAAAGGAGGGGAAATCTCGACATGGAAAAACT1360.10249839846252402No Hit
GTTTAAGGGATTCTGAGCCGAGCCCGGTGCCAAGCGCAGCTAGCTCAGCAGG1360.10249839846252402No Hit
CCATATGGGGCTTACAGCTTCTGTAGAACTATAAAACTTACTATGAACTGAC1360.10249839846252402No Hit
AAGCCAGGGCATGAACAGCTTTCTGTAGCAGAGATCACCAATGCTTGCTTTG1360.10249839846252402No Hit
ACACAGGGGGGGCGCCAGAGGGCTGCCCCTCGGCCGGTGACCGAGTTAAGCA1350.10174473376794663No Hit
TAAGCGGGGATTTTCTTTTTCTCCAACAGCAAGAATCTTTTGTTTTCATTTT1350.10174473376794663No Hit
TGATGCGGGCGATCTGTAGCTGGGCTGCCTCAGTCTCCCCACAGGCCTGCTG1350.10174473376794663No Hit
GGGTCTGGGGTGCTCAATAAACATCTGTTGATATTGTAGAATATCAGTAGTG1350.10174473376794663No Hit
GCATGCATGGTCCTCCAGCCAGAGACCGCGCTGGGAGAAATCAGGGGTTCAC1350.10174473376794663No Hit
GCAACTGGGATCATATTTGGTGAATGTCCATATTGTAGTAAGCCAATTACCT1350.10174473376794663No Hit
AACATTGGGGCGTGGTCTCAATTCTAGATCTACTATGCCAGTTTCTCTCTGG1350.10174473376794663No Hit
GGTACGGGGAATCATACAGTATGTGACTTTTGTGTCTGGCTTCTCTCACTTA1340.10099106907336926No Hit
AGAATCGGGACCTTGCATAGGTGATACACTAAACTATGTAGTAAACTGTAAT1340.10099106907336926No Hit
TGGTATGGGACAGATTTACAACCTAAATCTAGTCCTGCCACTTACTAGCAAG1340.10099106907336926No Hit
ATGCACGGGAACCTTGGGGCACTTTACAGACGTCAAGGCAAATTTGAAGCTG1330.10023740437879188No Hit
CTTCTTGGGATCGACATCTTCACCTCAAATACGTACGTGATGGTGGTCATGT1330.10023740437879188No Hit
GCCCAGGCATAATTTCAATTAATTTGCATTATCTAGGTAATTTTGATGAATG1330.10023740437879188No Hit
AAGACTAGGGAAGGACTGACGTTCAATCAAATATCTGAAAATACTAAAGGTC1330.10023740437879188No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)