FastQCFastQC Report
Sat 31 Dec 2022
SRR5849343.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849343.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131682
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGC9990.7586458285870507No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGCCGTCT5690.4321015780440759No Hit
TTTACTTAATAAACTGAAAAATTTTTAGGCAGTTTATATTCCTGTTGACTAG1880.14276818395832383No Hit
TATTCAGGGAGAGAAAGAAATAGTTCTATAGATAATTTCCCTAAGTAGCTTT1850.14048996825686125No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCCTGTCTCT1780.13517413162011513No Hit
CTACATTGGGGATTACCATTTCCTTACAAAAAGAAATTGATAATTGAAATTA1750.13289591591865252No Hit
GCACGGGACAGAAATGATGTTGCAGTTATGGTTATAGCACAAGGCAATTGCC1750.13289591591865252No Hit
AGCTAAGGGTGGAGAAATATTTAAAATTAGGTGGTTTTTTTCAAGCACGAGT1700.12909888974954814No Hit
GGTACGGGGAATCATACAGTATGTGACTTTTGTGTCTGGCTTCTCTCACTTA1690.1283394845157273No Hit
GCTTTGGGCAGATATGGAAAAATCACACTTCACAAGAGATCCATCCCAGCTT1680.12758007928190643No Hit
GCCCAGGCATAATTTCAATTAATTTGCATTATCTAGGTAATTTTGATGAATG1660.1260612688142647No Hit
TAGTCAGGGGACTTCTAGGTACTAAGAGTCAAGGAGGCAGGACTACATTTTC1650.1253018635804438No Hit
GACTTAGGGCGGGGCTGTGATTCTTCCCCTCTTGGGGCTGCTGCTCTCCCTC1640.12454245834662293No Hit
TAATAAGGGATTTATCTACATGTCTTTAGCATCTAGCAAATAATAGATGCTC1640.12454245834662293No Hit
AGTCTTGGGAATAACATAAAGCAAGTGAGCGACCTTATCTCTGTGCTTCGTG1620.12302364787898118No Hit
AGAATCGGGACCTTGCATAGGTGATACACTAAACTATGTAGTAAACTGTAAT1620.12302364787898118No Hit
TTATGTGGGAACTCAACGAGTCCTAACTTGGCAGCATGTATAATCATCGTTG1620.12302364787898118No Hit
GAACATGGGAGGCGCTAGTCTTAGCGCCCGGCCTATCTCATATTTTTATATC1610.12226424264516031No Hit
CAACTTGGGCAGGGTTTGTTCTCCTAACCACACCACCACACTGCCTGTAGAT1610.12226424264516031No Hit
AATTTAGGGACAGGCAGCTGCACACGTTACAGAAGCTACGCATGGATCTGAT1610.12226424264516031No Hit
CTTTTGGAAAGGTGGAGCCTGTGTCCTCTCCCTTTGAAACTGGACTGGCATT1590.12074543217751857No Hit
AAAATAGGGGATACTGTTTTAAATAATCTGTAATTTCAATTTTTTTTTTTTT1590.12074543217751857No Hit
GTACGAGGGGAGCACAGGGACTGATTTATGTTTTTTCTGCACAGATTTTATG1580.1199860269436977No Hit
GTAACAGGGGTTATGAAACCATAGAGTCTTGGTGAATATAGAGTAAAATATC1570.11922662170987681No Hit
CGAAACGGGGTAGTTTGGATACAAAGCTGATTAAATTGGATCAAGAAATATT1560.11846721647605594No Hit
CCATATGGGGCTTACAGCTTCTGTAGAACTATAAAACTTACTATGAACTGAC1550.11770781124223507No Hit
CGGTTTGGGGGAGAGAGGGAGCAGACAGACAATGCATCAAAGAGAATTCTCA1510.11467019030695161No Hit
AAGCCAGGGCATGAACAGCTTTCTGTAGCAGAGATCACCAATGCTTGCTTTG1510.11467019030695161No Hit
GTGCCAGGGAAAAATGTACACGTTTTTTCTTATTGTTACCACATGTTCACCT1500.11391078507313074No Hit
CTTGAGGGGCAGAAACCAAACCAGAACTGACATACATACCAGAATTGGCACA1490.11315137983930985No Hit
TGGTATGGGACAGATTTACAACCTAAATCTAGTCCTGCCACTTACTAGCAAG1490.11315137983930985No Hit
TTGTCGGGTCTAGCGGGGGCTGTTTTTTGTGCTTTCTTTTAAAAATATATAG1490.11315137983930985No Hit
AAGACTAGGGAAGGACTGACGTTCAATCAAATATCTGAAAATACTAAAGGTC1490.11315137983930985No Hit
TTTGTCCGGGTATAGGGAGAATAATTTAGATACACATGCTCAGAGCTGAGAT1480.11239197460548898No Hit
AGCTTAGGGAACATCTTTTTGTCTCCCAGCAGACTGACCCAGTGTCTTGTAC1480.11239197460548898No Hit
GGCTTTGGGATGGATAACAGCAAGTGTTGGCAAGGATATGGAGAAATTGGAG1480.11239197460548898No Hit
TTCTGGGAACTATCAGTTACATAGAATCTTTATGTGAAGTGTTTGTAAATAT1450.11011375890402637No Hit
GTTGGTGGGGACCTTGGACCATCATGCTTGGCCATATGGGTTTGAGACCTGG1450.11011375890402637No Hit
CGAACTGGGGTAGAAAACAGATAAGTGGCTTAGGCCAGTAGCAGGGGTAAGG1440.10935435367020549No Hit
TACATGGGGTGAATAGCTGGATTTATAAAGACTGAGCATAGGAGGAAATGGC1430.10859494843638462No Hit
AGCCGAGGGCTTACGGCCAAGTTTCTTCTAGTATGATGGAAAGTTTCTTTTT1430.10859494843638462No Hit
TCAGCAGGGATAGATGCCAGAAAAGAAGGTGCCGCTATAAAATGCTTTCTTG1430.10859494843638462No Hit
TAGCAAGGGGGTCTTCACTGACTGCACTTCTTCAGGAGAAGCTTTTGTTATC1430.10859494843638462No Hit
TGATGCAGGGGCAGGAAAGGAAAAGGAGGGGAAATCTCGACATGGAAAAACT1420.10783554320256375No Hit
CTTATACGGGGGGTGAAAGAAGGTTAGGATGTAGAAAAGGGATTTAGAGACA1410.10707613796874288No Hit
ACGCTGGGGCACAATTTTACTTATGTGCTTACGGACATCCAATAGTTTCATC1410.10707613796874288No Hit
TATGCCGGGTCAATAGATCAGTAAGGCTACATCCCTTTCTTGTGGTGGTAGG1410.10707613796874288No Hit
GCTGTTGGGGTACATTGGTAATTTAAACATCCAATAACAGGAAAATCTAGAT1400.10631673273492201No Hit
ACTGCTGGGACTACAGAAAGTTCTCTTTTCAGTAGGTGCTTACAAAATGGAC1400.10631673273492201No Hit
TATGGCTGGGAGAACAAGAAGGAAAGCTACCTGGGATAAATTCACGGTTTTT1400.10631673273492201No Hit
TGACCGGGAACCTTGAAACACAGGACATCTCATAATGCTGTAAGCAGGGACC1400.10631673273492201No Hit
AAATAAGGGCTAGCCAAATCCGGCCACAAGCGTCTCATCAGGGATCTCCAGC1390.10555732750110114No Hit
TTCATGGGGATAAAGACTCAATTCAGTATCACCTCGTGGTTTTATCTCATAG1390.10555732750110114No Hit
TGATGCGGGCGATCTGTAGCTGGGCTGCCTCAGTCTCCCCACAGGCCTGCTG1390.10555732750110114No Hit
AGCATAGGGGATTGACAGTGTCATAAACTTGAGAATTTGCGCAACTCTTCAG1390.10555732750110114No Hit
ATAGCGGGGACTGGCCGGGAGGAGCTAGAGGCGGGCGTCTGCACAGCGTCGC1390.10555732750110114No Hit
ACACACGGGCTTCCAAAAAGAAGAAAAATGTAAAAATAAGTAATCCAAATTG1390.10555732750110114No Hit
TTCACCGGGATCTAGATCATAAGCATAAAATAAATATTACAAAAATGATTGT1380.10479792226728026No Hit
TTATAGGGGATGCCTTGCGCAGTATCCTCACCATATCTCGACATGGCAACCA1380.10479792226728026No Hit
ACAGGAAGGGATGCCTGAGGAGAACTGTCAGTGTCCCTTCCGAGGGTCAGTT1380.10479792226728026No Hit
TCCAATGGGATGTATGGAGGCAAAAACATTAGTTCACACACAAACATTGTTT1380.10479792226728026No Hit
ATAACAGGGAGCCATTGGCTTTATGACTCTGGAAAGGTGCCTGGCACATGGA1380.10479792226728026No Hit
CAGGACGGGAGAGGTAAACAAATGTATTTTGGCTTCATTAAACATTTTGATA1370.10403851703345939No Hit
GAACTCGGGCTCCCCCTGATGAAATTTATGTGAGAACCGTCTACAACATCTT1360.10327911179963851No Hit
CAACCCAGGGATAAAATAATAGTGCTTTTGCAACTTACTGCATCTAAAATTT1360.10327911179963851No Hit
CTCGCAGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA1360.10327911179963851No Hit
TGGATGGGATCTTGGAAATTTCTGAAACTTCCCTCTTTCTGCCTATGTGTTA1360.10327911179963851No Hit
GCTCACGGGGACCAATATCTGGGACTTGTGAGGACTCACATAAATGCTGTTG1360.10327911179963851No Hit
TTCTGTGGGGAACACCAAAATGAGAATTAGGATTGATTCTGATTCCAACTTT1350.10251970656581764No Hit
TTCAGAGGGATGCCTGCCAAATTACACAGTAATTCAAATGGAAGGTAGAATC1350.10251970656581764No Hit
AATTTGGGGACGATGAACCTATTCTGAAGCACTTGAAAGATATTAAAGTGAA1350.10251970656581764No Hit
CATACCGGGACCATGGATAAATCATTTCATATTTTTGACCCTCAGTTTATTC1340.10176030133199677No Hit
CAAGCCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1340.10176030133199677No Hit
GGCATGGGGAGATTGGATTTGTGGAATCTCAATAATGACACAGAGGTACCAA1340.10176030133199677No Hit
CGAATAGGGGAACCAACACATAGTCCTTGTTGTCATTGACAGAACCCCAGTT1330.10100089609817592No Hit
CAGTTTGGGGAAAATCAGCAGAATTTCTTAATACTTCGCTGGTTCCTTCTTG1330.10100089609817592No Hit
ATGCACGGGAACCTTGGGGCACTTTACAGACGTCAAGGCAAATTTGAAGCTG1320.10024149086435505No Hit
GGGCCAGGGGGATATGAAGACTATGATTACCGATACAGAAGACGATCACCTT1320.10024149086435505No Hit
GACTCGGGGGTCAGGTTTTTTACCCATTTTTAAGATTGCCTTTTTGATTGAC1320.10024149086435505No Hit
TAATATGGGGCATGTATATATGTATTTTATACATTTTAAAGGTGTCTTAGTA1320.10024149086435505No Hit
TTCATCGGGTTCCTTTCCATTCCTTAGCAAGTATTTTCCATGAGTCATTACA1320.10024149086435505No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)