FastQCFastQC Report
Sat 31 Dec 2022
SRR5849342.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849342.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132244
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGC10360.783400381113699No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGCCGTCT5890.4453888267142555No Hit
AGCTAAGGGTGGAGAAATATTTAAAATTAGGTGGTTTTTTTCAAGCACGAGT1840.13913674722482683No Hit
AAAATAGGGGATACTGTTTTAAATAATCTGTAATTTCAATTTTTTTTTTTTT1770.13384350140649104No Hit
TGACCGGGAACCTTGAAACACAGGACATCTCATAATGCTGTAAGCAGGGACC1770.13384350140649104No Hit
TTATGTGGGAACTCAACGAGTCCTAACTTGGCAGCATGTATAATCATCGTTG1750.13233114545839508No Hit
GCTTTGGGCAGATATGGAAAAATCACACTTCACAAGAGATCCATCCCAGCTT1690.12779407761410727No Hit
TGATGCAGGGGCAGGAAAGGAAAAGGAGGGGAAATCTCGACATGGAAAAACT1670.12628172166601132No Hit
TTTGTCCGGGTATAGGGAGAATAATTTAGATACACATGCTCAGAGCTGAGAT1650.12476936571791537No Hit
GAACATGGGAGGCGCTAGTCTTAGCGCCCGGCCTATCTCATATTTTTATATC1650.12476936571791537No Hit
TTTACTTAATAAACTGAAAAATTTTTAGGCAGTTTATATTCCTGTTGACTAG1640.12401318774386741No Hit
AGCTTAGGGAACATCTTTTTGTCTCCCAGCAGACTGACCCAGTGTCTTGTAC1640.12401318774386741No Hit
AACATTGGGGCGTGGTCTCAATTCTAGATCTACTATGCCAGTTTCTCTCTGG1610.12174465382172348No Hit
CTTGAGGGGCAGAAACCAAACCAGAACTGACATACATACCAGAATTGGCACA1600.12098847584767553No Hit
TAATAAGGGATTTATCTACATGTCTTTAGCATCTAGCAAATAATAGATGCTC1600.12098847584767553No Hit
AAATATGGGGCAGATATGAGGTCTTAGACTGGGAACTGTCCCACACAAGAGC1600.12098847584767553No Hit
AAATAAGGGCTAGCCAAATCCGGCCACAAGCGTCTCATCAGGGATCTCCAGC1590.12023229787362755No Hit
CTACATTGGGGATTACCATTTCCTTACAAAAAGAAATTGATAATTGAAATTA1590.12023229787362755No Hit
CAACCCAAGGATAGATGGTAACAGTTCAAATATTGTTTTGAATACAATGGAA1580.11947611989957957No Hit
TGATGCGGGCGATCTGTAGCTGGGCTGCCTCAGTCTCCCCACAGGCCTGCTG1580.11947611989957957No Hit
ACAGGAAGGGATGCCTGAGGAGAACTGTCAGTGTCCCTTCCGAGGGTCAGTT1580.11947611989957957No Hit
TAGCAAGGGGGTCTTCACTGACTGCACTTCTTCAGGAGAAGCTTTTGTTATC1560.11796376395148361No Hit
ACGCTGGGGCACAATTTTACTTATGTGCTTACGGACATCCAATAGTTTCATC1550.11720758597743566No Hit
TATTCAGGGAGAGAAAGAAATAGTTCTATAGATAATTTCCCTAAGTAGCTTT1550.11720758597743566No Hit
CGAATAGGGGAACCAACACATAGTCCTTGTTGTCATTGACAGAACCCCAGTT1520.11493905205529173No Hit
TATGGCTGGGAGAACAAGAAGGAAAGCTACCTGGGATAAATTCACGGTTTTT1520.11493905205529173No Hit
GCTGTTGGGGTACATTGGTAATTTAAACATCCAATAACAGGAAAATCTAGAT1510.11418287408124375No Hit
CTTGCCATGCGGAGGGGATATTCAAGAGAAGCTTATGTGGAGTTAGTTCACC1510.11418287408124375No Hit
GGTACGGGGAATCATACAGTATGTGACTTTTGTGTCTGGCTTCTCTCACTTA1500.11342669610719577No Hit
ATTAAAGGGATCTCACAATTATATTAGCAGTGGTAACATGGGAAGTGAAGGG1480.11191434015909985No Hit
GCCCAGGCATAATTTCAATTAATTTGCATTATCTAGGTAATTTTGATGAATG1470.11115816218505187No Hit
GTAACAGGGGTTATGAAACCATAGAGTCTTGGTGAATATAGAGTAAAATATC1450.10964580623695593No Hit
CAACCCAGGGATAAAATAATAGTGCTTTTGCAACTTACTGCATCTAAAATTT1450.10964580623695593No Hit
TACATGGGGTGAATAGCTGGATTTATAAAGACTGAGCATAGGAGGAAATGGC1450.10964580623695593No Hit
AATTTAGGGACAGGCAGCTGCACACGTTACAGAAGCTACGCATGGATCTGAT1450.10964580623695593No Hit
GCACGGGACAGAAATGATGTTGCAGTTATGGTTATAGCACAAGGCAATTGCC1440.10888962826290796No Hit
CGTGACGGGAAGAGCAATGTTTTGCTTTGCTCTCTGGTCCATGAACTCATTT1430.10813345028885998No Hit
ACAAGCGGGATCTTGGGAGCTTACATTAGGATATAGAGACCAGTAAAAGTAA1430.10813345028885998No Hit
AAGACTAGGGAAGGACTGACGTTCAATCAAATATCTGAAAATACTAAAGGTC1430.10813345028885998No Hit
GCTAAAGGGATATAGAATAATATTTATGTTTACAATGTAACTTTTCAAAATT1420.107377272314812No Hit
GTCTCACAAAAAAGAAAAGAAAACAAGAAAAAGAAAAATTGTGTATTGAATT1420.107377272314812No Hit
TTCAGAGGGATGCCTGCCAAATTACACAGTAATTCAAATGGAAGGTAGAATC1420.107377272314812No Hit
TCTCTTGGGTGGTTTTAAAAGAGACTATGATGTAAGGAAGCAACTGTTGACA1410.10662109434076404No Hit
CAACTTGGGCAGGGTTTGTTCTCCTAACCACACCACCACACTGCCTGTAGAT1410.10662109434076404No Hit
GATTCCGGCAGTAAGAAAAATTTAATTGATGATGGATTATTAATGCTCAAAG1410.10662109434076404No Hit
CGGTTTGGGGGAGAGAGGGAGCAGACAGACAATGCATCAAAGAGAATTCTCA1400.10586491636671606No Hit
ACTGCTGGGACTACAGAAAGTTCTCTTTTCAGTAGGTGCTTACAAAATGGAC1400.10586491636671606No Hit
GTACTGGGGTAGTTGAAAAAAGAAGTTTGGGCTAACATTTAGGAGAAGTTGG1390.1051087383926681No Hit
TGGTATGGGACAGATTTACAACCTAAATCTAGTCCTGCCACTTACTAGCAAG1390.1051087383926681No Hit
ATGTCCGGGATAGATAATAGGGCTATTCATATTTTCTTTTTGCAACTGTGTT1390.1051087383926681No Hit
TTTCCAGGGGGTATTAACCAAATACATTTGTAAAGAATCAATAACAAAGAAC1390.1051087383926681No Hit
TCTGTGGGATTACTCATTGCTACGGCAGTTCAAAATGACTTGGAAAAAAACA1380.10435256041862014No Hit
AATCTTGGGGTCCCTGAAGGTCGTGTGCTGCATGATCCCATTTGCATGACGT1380.10435256041862014No Hit
TCAGCAGGGATAGATGCCAGAAAAGAAGGTGCCGCTATAAAATGCTTTCTTG1380.10435256041862014No Hit
CATGGTGGGCCGATCTGCGTGAGGGCATTAGAGTGACCTTGAGCAAGTTACT1380.10435256041862014No Hit
ATCCAAGGGATGAAAATAGTGGCTAGGCTAGTGTGATTTTAAGTATGTCTGG1370.10359638244457216No Hit
AGAATCGGGACCTTGCATAGGTGATACACTAAACTATGTAGTAAACTGTAAT1370.10359638244457216No Hit
TATTGAGGGTAGTTCCTGCTACTCAGGAGGCTGAAGCAAGAGGTTTGCTTGA1370.10359638244457216No Hit
TCAGTAGGGGCAGGAGCTGTGTTTATCTTGGTTGTCACTGTGTCCCTAGGAA1370.10359638244457216No Hit
TTCACCGGGATCTAGATCATAAGCATAAAATAAATATTACAAAAATGATTGT1360.10284020447052418No Hit
AGTCTTGGGAATAACATAAAGCAAGTGAGCGACCTTATCTCTGTGCTTCGTG1360.10284020447052418No Hit
TGTCTTGGGAGTGACCCAACATTTCAGCAAGGAGGTACTCATTGGGAAAAAT1360.10284020447052418No Hit
CTTCTTGGGATCGACATCTTCACCTCAAATACGTACGTGATGGTGGTCATGT1360.10284020447052418No Hit
TTCATGGGGATAAAGACTCAATTCAGTATCACCTCGTGGTTTTATCTCATAG1360.10284020447052418No Hit
CAGTTTGGGGAAAATCAGCAGAATTTCTTAATACTTCGCTGGTTCCTTCTTG1360.10284020447052418No Hit
GTACGAGGGGAGCACAGGGACTGATTTATGTTTTTTCTGCACAGATTTTATG1350.1020840264964762No Hit
TAATCAGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT1350.1020840264964762No Hit
AAGTCGGGCACATCGGGGACCTGGCAGGAATCCTGACCCATGCCCTTGAAGC1350.1020840264964762No Hit
TTGCCAGGGAAGGCGTTTAGATTTTTTTTGTTTGACTTATTTCCCTCAAGGT1340.10132784852242825No Hit
TACATAGGGACTGATTTGTTTGAATTTGAAGGCCATGTGAAATACTTATGTG1340.10132784852242825No Hit
GTTGGTGGGGACCTTGGACCATCATGCTTGGCCATATGGGTTTGAGACCTGG1340.10132784852242825No Hit
ACATTTGGGGGAGAGAAACCATATAAATGCAGTGATTGTAAGAAAGCATTCG1340.10132784852242825No Hit
TAGTCAGGGGACTTCTAGGTACTAAGAGTCAAGGAGGCAGGACTACATTTTC1340.10132784852242825No Hit
ATAACAGGGAGCCATTGGCTTTATGACTCTGGAAAGGTGCCTGGCACATGGA1340.10132784852242825No Hit
CGAAACGGGGTAGTTTGGATACAAAGCTGATTAAATTGGATCAAGAAATATT1330.10057167054838027No Hit
TAAACGGGATTTTGGAAGCAGAATTAAGCAGAGGAGATGGTGATGTCGCTTG1330.10057167054838027No Hit
GTTTAAGGGATTCTGAGCCGAGCCCGGTGCCAAGCGCAGCTAGCTCAGCAGG1330.10057167054838027No Hit
AGCTCTGGGACACCATTGCTACGTTAGATTTGAAATGACATCTCTGTTAAAA1330.10057167054838027No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)