FastQCFastQC Report
Sat 31 Dec 2022
SRR5849341.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849341.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144982
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGC10830.7469892814280393No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGCCGTCT10270.7083637968851306No Hit
GTTCCGGGTCTTCCTGGAATTGGATTTTATGTTAAACAACTTCTAAGGCCAG2270.15657116055786235No Hit
ACTCCGGGTGGAGGCTGGAGTAATCAGATTACTGCTTTAATCAGAAACCCTC1820.12553282476445352No Hit
CGCTACGGGCCTTGTTCAAGTCTATGTTATGGATTTTGCTTCTTTCTAAAGT1800.12415334317363534No Hit
GAGACAGGGTAGATGGCATCTTTTTCCAATAGAAGACTGCTTCATCTACACT1710.11794567601495357No Hit
TCTGACGGGAGTGAGCCAAACTTTCAAGAAGACAGCTGAGAAAGCATACCTC1620.11173800885627182No Hit
TGTGGCGGGAGAGTCTAAAATTGGATCTGAATCCAGTATTTGCCAGCCAGTG1590.10966878647004456No Hit
CCCCTACCAGCAGATTCTGCGAAAGCACCAAATTTCTCAAGACCCTCTTCTC1570.10828930487922638No Hit
ATACGCGGGACCGAAGGACATTATCAAGAAAGCGGAAAGACATCTTACAGAA1560.10759956408381731No Hit
AGCCTAGGGAGTATTTATAAAGCAAAAGAGCACTGTAGTATGAAAATTGTAT1560.10759956408381731No Hit
TTTAGCGGGACCCCAGAACTTAAAAGTAGAGGGGAAAATATAAAATAAAATT1560.10759956408381731No Hit
TCTGAAGGTCCCTCCTGGATTTTTATCCTGACATTGTAAAGCATGGAGCCAA1560.10759956408381731No Hit
CGGCCTGGGCCAAAATTTAAAGTATGCTTGCTCCCCACCCACCAAACCTTAA1550.10690982328840822No Hit
GCAAACGGGGGGAGTTCATGAACTAATTAAATTGACTTTAGTAAAAGTCAAA1550.10690982328840822No Hit
TGCACAGGGGTGCACATTCAATGTGTGTTAAGTGTGATTCGGCTAAACCAGG1540.10622008249299913No Hit
ATTCAGGGCGATCTTACAGACGGGTGACTGAATTAGGAATTTGGATGCTTAT1540.10622008249299913No Hit
GATGGCGGGGATTTGTCTGGGCATAATTTTATCAAATCAGATAAATGCTGCT1530.10553034169759004No Hit
GCATTTGGGGATGTCAGTGGAAAAAAATGAAAACAAAATCATAATAAAAGTA1530.10553034169759004No Hit
ACATATGGGCTTGAAAGGAGGGTAAAAAGACTAAAATGAGGGGGAACAGAGT1530.10553034169759004No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCCTGTCTCTTATAC1510.10415086010677188No Hit
TACCACGGGATATAAGGAACTTACAATTCAGTAGCAGAAAAACAACCTGATT1510.10415086010677188No Hit
CGAATAGGGATTTCCTAGCCTGCTATTTGAGGCTTATTTTCTCTCTCATTCT1500.10346111931136279No Hit
GGTTATGGGTTGGCGGTTGGCTTGTCGCGAATCTTCATCTTAGCACATTTCC1500.10346111931136279No Hit
TAAATTGGGCATCATAAATAGAAAAGCCAGATATGGTAACACCGCGATGACT1500.10346111931136279No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCCTGTCTCT1490.10277137851595369No Hit
TCAAATGGGGCTGGAGCCCATTCAGATAAAACTGCCCAATACTGAGAGGTGT1490.10277137851595369No Hit
GAACCGGGGTCCTCCAGGTTCATCCACGTTACAGCATGTGACAGAATTTTCT1480.10208163772054463No Hit
ATTGCAGGGGGAAATTATAGTAGCTTTGGTTAGGAATAATATCTTCTAAGTG1470.10139189692513553No Hit
CCTAAAGGGCAAGGAAGTAATGAATTCAGAAGAAAAGACTAAAAATGGTGTA1460.10070215612972644No Hit
AGTACTGGGATTCACGATAGGATACAAAGTGTGATTTGTTGGAAACATGTCC1450.10001241533431737No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)