FastQCFastQC Report
Sat 31 Dec 2022
SRR5849339.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849339.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145711
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGCCGTCT10870.745997213662661No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGC9820.6739367652407848No Hit
GTTCCGGGTCTTCCTGGAATTGGATTTTATGTTAAACAACTTCTAAGGCCAG2140.14686605678363335No Hit
GAGACAGGGTAGATGGCATCTTTTTCCAATAGAAGACTGCTTCATCTACACT1810.12421848727961513No Hit
TGTGGCGGGAGAGTCTAAAATTGGATCTGAATCCAGTATTTGCCAGCCAGTG1760.12078703735476387No Hit
CAGCCAGGGAGAGGATGAACCTGCTATTTTCATCTCTTCTTGGTGGGAAGAA1740.11941445738482337No Hit
TCTGACGGGAGTGAGCCAAACTTTCAAGAAGACAGCTGAGAAAGCATACCTC1690.11598300745997213No Hit
CGAATAGGGATTTCCTAGCCTGCTATTTGAGGCTTATTTTCTCTCTCATTCT1670.11461042749003163No Hit
TAATGTGGGAGAAGAGATTAAAAATCAATGTGTTACTCCCCAAATAATCTAT1640.11255155753512089No Hit
ACAGCCGGTGGTCAGTGTGCAAAGAAAAGAACTGCTTGCATTTCTTTATTTC1640.11255155753512089No Hit
GGTTATGGGTTGGCGGTTGGCTTGTCGCGAATCTTCATCTTAGCACATTTCC1640.11255155753512089No Hit
ACAGTCGGGTTCCTGTAACAGCATTTCTCCTGTATCTGAATTCTGCCTTACT1640.11255155753512089No Hit
GATGGCGGGGATTTGTCTGGGCATAATTTTATCAAATCAGATAAATGCTGCT1630.11186526755015064No Hit
TTTGTCGGGGTAGTGCGTAGAATATGCATCAATTTCTTGAAGGAGATTCATG1630.11186526755015064No Hit
TCTGAAGGTCCCTCCTGGATTTTTATCCTGACATTGTAAAGCATGGAGCCAA1620.11117897756518039No Hit
ATCACAGGGGTAGCTAATAATTAGTTGTAAAAATCATTAAGATGGAATTGAC1610.11049268758021014No Hit
GTTAAGGGGGGCTAGTATAGGAAGTGAGAAGTTTTTCGTAGATTTTTTTTTT1570.10774752764032913No Hit
GCACTAGGGAGAAGGAATATGCACTGCTGTCAAAAAGTTGCAATGTAAAATA1570.10774752764032913No Hit
CGGCCTGGGCCAAAATTTAAAGTATGCTTGCTCCCCACCCACCAAACCTTAA1550.10637494767038866No Hit
ACTCCGGGTGGAGGCTGGAGTAATCAGATTACTGCTTTAATCAGAAACCCTC1550.10637494767038866No Hit
GTTCGTGGGCAAGGTCGCTGGTCATCCTCTGGCACAGAACGAACGTTGTCTT1530.10500236770044816No Hit
GTTTAGGGGGGATTATTAATGCTCAAAGAAAAATAATTAAAGGAAAGATTTC1520.10431607771547789No Hit
TGTGAGGGGTTTTGAGTCATCCAGTCCTGGGCACTGAGGTCTGTTAGATGAA1510.10362978773050766No Hit
AGCCTAGGGAGTATTTATAAAGCAAAAGAGCACTGTAGTATGAAAATTGTAT1510.10362978773050766No Hit
ATGCAGGGGTGATAAAATAGAGGATTTTAAATTTTGTATAGTATTTGTTAAT1510.10362978773050766No Hit
GAGATAGGGGATTTTTTGTTCATTCTGATTTGTCATTTTATTATCTGTTATC1510.10362978773050766No Hit
TCAGCAGGGATACTAGCAATACATTGAAACTAAAAATAAAATTCCAATATTA1500.1029434977455374No Hit
TAAATTGGGCATCATAAATAGAAAAGCCAGATATGGTAACACCGCGATGACT1500.1029434977455374No Hit
GGTGCCATAGGGAAGGTGGAAAAAGTTTCCTAAAATAAACCATTTTGATAAA1500.1029434977455374No Hit
CGCTACGGGCCTTGTTCAAGTCTATGTTATGGATTTTGCTTCTTTCTAAAGT1500.1029434977455374No Hit
CTGTATGGGGAAAATAAGAGAGCTGATTTCTGTTAAAAACTAGGCCTTGTGT1470.10088462779062665No Hit
GCAGAATGGGCCTAGCCTCAGCTGTGACACCTCCATCCCCACTTCAGAGCTT1470.10088462779062665No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCCTGTCTCTTATAC1460.1001983378056564No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)