FastQCFastQC Report
Sat 31 Dec 2022
SRR5849332.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849332.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162553
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGC8160.5019901201454295No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGCCGTCT6620.40725178864739503No Hit
CGCTATGGGAGGGGGACATGTTTTCATTTCTCTTGGATGTATACCTAGAAGT1970.12119124224099216No Hit
TAGACTGGGGACACAGAAAGATCACCTACTGCATACGCCAAAGAAAGATCAC1770.108887562825663No Hit
AGTGGCGGGATCACAGTTAGAAGGAAATGATAGTCAAATACACGTTTAGATT1730.1064268269425972No Hit
CCAGCCTGGGCAATAGAGTGAGACTCTGTCTGGAAAATAAAAAAGAAAATTC1730.1064268269425972No Hit
ATATACGGGGTTTGGAAATGTTTTTCGTTTTTGTCATAAAAGAAACATGATT1720.10581164297183071No Hit
TGACGTAGGGGGAAGCTAAGCATGAACGCATTGAAAACGATGTTGCCACCAT1720.10581164297183071No Hit
TGCTATCGGGTACCTAGGAATACATCTGCCAAGGAGATGAAAGATCTCTACC1690.10396609105953135No Hit
TGTAAGGGGATTGAACTTTCTTAAATAATTCTATAACAAATTCTCTTCTTTT1650.10150535517646551No Hit
TAACACGGGATTTTCTTGGATGAAGAGGGATATCAAAAAAGCTTAAAAGCAA1640.10089017120569906No Hit
GCTACGGGGGAAACATAAATTTTAGGTGGAAGTCAGTTTTAGAGAAGGAGTG1640.10089017120569906No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)