FastQCFastQC Report
Sat 31 Dec 2022
SRR5849331.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849331.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162292
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGC7880.48554457397776846No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGCCGTCT6580.40544204273778134No Hit
ATATACGGGGTTTGGAAATGTTTTTCGTTTTTGTCATAAAAGAAACATGATT1980.12200231681167278No Hit
GACACCATTCTCTCTCCATTTACTTCTGGTGGTTACCCTGACTCTTGACTCT1800.11091119710152071No Hit
TAACACGGGATTTTCTTGGATGAAGAGGGATATCAAAAAAGCTTAAAAGCAA1750.10783033051536736No Hit
TGCCAAAGGGGAATGTGCTTGCCTGTCAGGTGCAGGGAGAATTCAGTGAGAT1720.10598181056367537No Hit
CGTTCTGGGGGAATGAAGAAAGGGAGTGAAGAAGACAGGGAATGTAGGAATG1700.104749463929214No Hit
TGACGTAGGGGGAAGCTAAGCATGAACGCATTGAAAACGATGTTGCCACCAT1690.10413329061198333No Hit
ATAGCAGGGAAGAGAGGTAGGAATTATAACATTTTAAATTTCTGTGTTGGAA1690.10413329061198333No Hit
CGCTATGGGAGGGGGACATGTTTTCATTTCTCTTGGATGTATACCTAGAAGT1670.10290094397752199No Hit
TCAGAAGGGATTCTCAGACACTTTCATACCACGCTTTGAAAATACTGGTTTC1660.10228477066029132No Hit
AACAGGGGGTATGTAAATAAATAAATGTAATATTTCATCCTTTATTTTTCAG1660.10228477066029132No Hit
AGCAGTTGGCAATTTGTAAACAAGTACTTCATGGTGGTGAGGTAAGGAGAAG1640.10105242402582998No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)