FastQCFastQC Report
Sat 31 Dec 2022
SRR5849320.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849320.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141159
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGC9960.7055873164304083No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGCCGTCT9890.7006283694273833No Hit
CACAGAGGGAGAATTTAAAATACCAGTAAACTAGATCTGATATAGAAGATGT2120.15018525209161301No Hit
TGTTATGGGAAAGATGTACATTGAGGGAATATCAAAAAATAGCGTTCATGGC1960.1388505160846988No Hit
TGTTCCGGGTTTCAGAGACATCTCAAATTGAAAGAATATCAGATTGCTTTTA1940.13743367408383453No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCCTGTCTCTTATAC1790.1268073590773525No Hit
AGTTCAGGGGAGAGACAAGACATGGACTAGACTTCCAAAGGTTGATTTATGA1780.12609893807692035No Hit
GGCAACGGGATATCTGAGAAATGGTTTAATTCATGCTCAGGAGCTGTGTGCC1770.1253905170764882No Hit
GCTGAAGGGATACAAAACAACCAGAAAACAATTAACAAAATGACAAAAATAA1750.12397367507562394No Hit
CAGACTGGGCTCTAAGATGTGCATTGTGATCAATGACAGGAAAGTAAAAACA1730.12255683307475966No Hit
TAGATCGGGATCTTAACTGGAAACAGAGTTTCTCAACCTTCAGATATTGAAA1730.12255683307475966No Hit
GTATTTGGGACCAGTTCTTAGAAGTATTGATCTCCAAGAAAGAGCAGAAAGA1680.11901472807259898No Hit
GTCCACGGGCTGAATGTTTCATATTTTCTTGAAGCAGCATGTAATACTTTTA1670.11830630707216686No Hit
TGATTAGGGTGCCTTCTGCAGGGAAGGAGCAGTCAGTGTTGTGGGTGTCCCT1620.11476420207000616No Hit
GCACTGGGGACAATTTAACCCATAACACACTGTATCCTGAAATAACTATCCA1620.11476420207000616No Hit
CCTAATTGGGATTGACCAAAGTAAGAAAATGCAAGTTCTAAAAATAGACTAA1610.11405578106957404No Hit
GTCGGCGGGGATCTTTCCGCGGGGATGGTGTGTTATGGGAAGACTTCTTAGA1600.1133473600691419No Hit
GCTAAAGGGCTCCTGCCTCAGCCTCCCAAGTAGCTGGGACTACAGGCGCCCG1600.1133473600691419No Hit
AGTCAAGGGCAACAACTTCAGTAAAATGGCAGAAGAGAAACTGACCCACAAA1580.11193051806827761No Hit
GGCCGTGGGCGTGCAAAGACACACATAGGCTCAAAATAAAGGGATGGAGGAT1580.11193051806827761No Hit
TCTTTGGGGGGCCCCTTTAACCTTTAGGGTGCGCGGGTGCGGTATATCTCGC1570.11122209706784549No Hit
ATACGAGGGTAGCACGAGCTGAGTTGAAAGATAATTCAAAAATGTCTTCATA1560.11051367606741333No Hit
TAAACCGGGACTTCAGACAAGGATTCATGAATCAGGCAAATCAAACTATAAG1550.1098052550669812No Hit
ATAATGGGGATGGTTCTTTATGAAAACAGTATTTTTTTATAAAACTGATCAA1540.10909683406654908No Hit
GCAAAGGGGGTGAAAACTGAATTCTAAGGAAATAATGACGTCATGACTTATT1530.10838841306611693No Hit
TATCCCAGGGGGCCGCCATCGCGGGTGCGCGGGCGCAGAGCCTGGAATCCGG1530.10838841306611693No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTGTCTCTTATACA1530.10838841306611693No Hit
GTGATTGGGTAGAAAAACAAAAATAATAAAAGTGTTCCCATCCAGAGAGAAC1530.10838841306611693No Hit
TATCCTGGGAGAGGTGGTGTCTTTAGTTGACAGTTCACAAACTTGTGTAAAG1530.10838841306611693No Hit
AATAGCGGGTCAGAAACAATCGGCCCAGAAGGTTATATTTAAAAAGAAAGTT1530.10838841306611693No Hit
TATAATGGGGTCAAGTGAAGGCTGCCCTGAGCTCCTATAACTTTTCTCAGAT1530.10838841306611693No Hit
GTACCGGGTAAATCCATTAGGTTACTCCTGCAGCATGCGCTTTTAGCTTCTC1510.10697157106525265No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACA1500.10626315006482052No Hit
TTTGGTGGGTAAATAAATGAAGAGGTATGCCATATTGATGGATTAAAAGATT1490.10555472906438837No Hit
TATCGTGGGTGTATTTGGTGGGGAAAACCTAATTTCGGGGATTTCTGTGGTA1490.10555472906438837No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCCTGTCTCT1490.10555472906438837No Hit
ACTGCAGGGTCTGGTTAAGGGGTAGCCTTCAGTGAGATTTCCATCTTGGCTG1480.10484630806395624No Hit
AGAAGTGGGATGTCGAAGTAATTTATGAGTTGACTTTTATACTTAGGCTTCT1480.10484630806395624No Hit
CCCAATGGGGAGGATCTGTGCCATCTTGGATTGAGAATTGCAGATGTGACAG1480.10484630806395624No Hit
TAGTCTGGGATTCTCAAACCCTGAAGCAAATGAGAAAGGAGAGAGTGTTATT1480.10484630806395624No Hit
TGACTGGGGAGTAAAAGTCTGATGCCAAGACAAATATTGGGAGCGAGCCTTC1470.10413788706352411No Hit
GCTTCGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1470.10413788706352411No Hit
TTAGCAAGGGCCAACTCTCAGAATTCAGACTTCCTGCAGCTGCCATATCACC1460.10342946606309199No Hit
TTATGAGGGGCCACGAGTCTCCTGGGCATCTGTCACTTCTTAGCTTCTCAGC1460.10342946606309199No Hit
ATTTGCGGGCTAGGTGCTTATCAGATATACGAATTGTAAATATTTTCTCTCA1460.10342946606309199No Hit
TGCTTTGGGGAATTACAAGGAATGATTCCAGATTCTTGCCATCAATAAGCCT1460.10342946606309199No Hit
GCTCAAGGGGCAGTGGAATATATTTGAAAATGGATCACCAATTGATAGTTAG1460.10342946606309199No Hit
AGCACGGGGGAATTATGAATAAAGAATTCTTACAACTTAAAATAAATAAAGC1450.10272104506265983No Hit
TCCCAAGGGATCATATCAACGTTTCTTCACGTCCATCCTTTTGGAGCCAACA1450.10272104506265983No Hit
TTGTTAGGGGGGAAGTTTCCTTAATAAAAGTGTGACGATTAAAGCTCTGGTG1440.10201262406222769No Hit
CATTCCGGGGTAATTCTAAATTTTCCCAGATAATACTTAGAATGAATTCCCA1430.10130420306179556No Hit
CATAGGGGACCAAGAACGTTGGCAATGAAAATTGAGCATTTTTTCTGGAAGA1430.10130420306179556No Hit
CAGGTAGGGGAAAAAAAGCAGAAATGATCATTCATTTAGTGCTAACCAACTA1420.10059578206136344No Hit
GTTTATGGGACCCATCATAATGGTCACTGCTGACTCTCATATAACAAAGGCA1420.10059578206136344No Hit
AGCCTGGGCTACAGAGCAAAACTCTGTCTCAGAGTAAGAAAAGCTACCACAT1420.10059578206136344No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)