FastQCFastQC Report
Sat 31 Dec 2022
SRR5849319.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849319.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140502
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGC10820.770095799348052No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGCCGTCT9350.6654709541501188No Hit
CAGACTGGGCTCTAAGATGTGCATTGTGATCAATGACAGGAAAGTAAAAACA2080.14804059728687138No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCCTGTCTCTTATAC1990.14163499451965098No Hit
TGTTCCGGGTTTCAGAGACATCTCAAATTGAAAGAATATCAGATTGCTTTTA1860.1323824571892215No Hit
TGATTAGGGTGCCTTCTGCAGGGAAGGAGCAGTCAGTGTTGTGGGTGTCCCT1850.13167072354841924No Hit
GCTGAAGGGATACAAAACAACCAGAAAACAATTAACAAAATGACAAAAATAA1850.13167072354841924No Hit
AGTCAAGGGCAACAACTTCAGTAAAATGGCAGAAGAGAAACTGACCCACAAA1800.12811205534440792No Hit
ATTTGCGGGCTAGGTGCTTATCAGATATACGAATTGTAAATATTTTCTCTCA1760.12526512078119884No Hit
TACCACGGTGTGGTGAAGATTGTATTAAACTATATAACTCAAGACTACACTT1730.12312991985879204No Hit
TAAACCGGGACTTCAGACAAGGATTCATGAATCAGGCAAATCAAACTATAAG1730.12312991985879204No Hit
TAACAAGGGGCAGCCAACGTGGTGGGACCCAAAATCTGCCTGGAAGATAATG1730.12312991985879204No Hit
TATCCCAGGGGGCCGCCATCGCGGGTGCGCGGGCGCAGAGCCTGGAATCCGG1720.12241818621798978No Hit
CACAGAGGGAGAATTTAAAATACCAGTAAACTAGATCTGATATAGAAGATGT1710.12170645257718751No Hit
AGTTCAGGGGAGAGACAAGACATGGACTAGACTTCCAAAGGTTGATTTATGA1700.12099471893638526No Hit
TACTTAGGGCATTGAAAATTTCTCGTTTTCATTTTCTTAGATGACTTCTTGT1660.11814778437317618No Hit
TATCCTGGGAGAGGTGGTGTCTTTAGTTGACAGTTCACAAACTTGTGTAAAG1650.11743605073237391No Hit
TAGTCTGGGATTCTCAAACCCTGAAGCAAATGAGAAAGGAGAGAGTGTTATT1650.11743605073237391No Hit
GCACTGGGGACAATTTAACCCATAACACACTGTATCCTGAAATAACTATCCA1640.11672431709157166No Hit
GTGCAAGGGATGAAATAGGCCCCCCAAAATAATACAACTTGTTTATGGGAAA1630.11601258345076938No Hit
GGTGCAGGGGTCACCAATAGCCATGTGGTTAGTCCAGGGAACCAGTTTGTTA1630.11601258345076938No Hit
TCTTTGGGGGGCCCCTTTAACCTTTAGGGTGCGCGGGTGCGGTATATCTCGC1630.11601258345076938No Hit
TGTTCTGGGAGTCTTCCAGCTAGCAGGACAGACTATTTTTTTCTGACAAGAT1620.11530084980996712No Hit
AGAATAGGGGTTCACTTGAGGAGGGAACTTCATGTTTAACATGTATTTGCAA1620.11530084980996712No Hit
GTCGGCGGGGATCTTTCCGCGGGGATGGTGTGTTATGGGAAGACTTCTTAGA1610.11458911616916485No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCCTGTCTCT1610.11458911616916485No Hit
GCACTTGGGACATCAAAAGGTCTTTTCAGGACACAGCATGCAAATGTGCCAT1600.11387738252836258No Hit
TTTAGTGGGTAGAATCATCTAGAATAAAGTGATGGCAGTAGCCTCTCTAAAG1600.11387738252836258No Hit
ATACGAGGGTAGCACGAGCTGAGTTGAAAGATAATTCAAAAATGTCTTCATA1600.11387738252836258No Hit
CTACATGGGCTCGGCTAGTATTATTTTAATAATTTGCTAATCACCTCAAAAT1590.11316564888756031No Hit
GCTCAAGGGGCAGTGGAATATATTTGAAAATGGATCACCAATTGATAGTTAG1580.11245391524675806No Hit
TGTTATGGGAAAGATGTACATTGAGGGAATATCAAAAAATAGCGTTCATGGC1570.11174218160595578No Hit
TAGATCGGGATCTTAACTGGAAACAGAGTTTCTCAACCTTCAGATATTGAAA1570.11174218160595578No Hit
GTCCACGGGCTGAATGTTTCATATTTTCTTGAAGCAGCATGTAATACTTTTA1560.11103044796515352No Hit
CTATAGCGGGCACTATTTTTTCCATTTAGCTAGAAATCTAGAGGCTTTTTCA1560.11103044796515352No Hit
CTAAATGGGGATACAATCAGCAACAGCAGTATCAAATAATGTTTATTAAGCT1550.11031871432435125No Hit
GTGATTGGGTAGAAAAACAAAAATAATAAAAGTGTTCCCATCCAGAGAGAAC1550.11031871432435125No Hit
TGACTGGGGAGTAAAAGTCTGATGCCAAGACAAATATTGGGAGCGAGCCTTC1540.109606980683549No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTGTCTCTTATACA1530.10889524704274672No Hit
TGGAACCGGGTATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC1500.10676004612033992No Hit
TTGAACGGATCAGTTGGATGATTCTCACCTTTACTCCTCAGTAAATCAGGAA1500.10676004612033992No Hit
GTATTTGGGACCAGTTCTTAGAAGTATTGATCTCCAAGAAAGAGCAGAAAGA1500.10676004612033992No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACA1490.10604831247953767No Hit
GACTCAGGGCTCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGG1480.1053365788387354No Hit
TCGTAGGGGCAGAGCAGGAGGGAGGCAAGCCGCCTCTTCTCGGCCATCGACT1480.1053365788387354No Hit
AGAAGTGGGATGTCGAAGTAATTTATGAGTTGACTTTTATACTTAGGCTTCT1470.10462484519793312No Hit
TTTGGTGGGTAAATAAATGAAGAGGTATGCCATATTGATGGATTAAAAGATT1460.10391311155713086No Hit
GGCCGTGGGCGTGCAAAGACACACATAGGCTCAAAATAAAGGGATGGAGGAT1460.10391311155713086No Hit
GCTTCGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1460.10391311155713086No Hit
GCTAAGGGGTAACTGTATTATGTAACTATTTTACCAAAAATCTAAGGATACA1460.10391311155713086No Hit
CTCAACAGGGGAATTAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGG1460.10391311155713086No Hit
GACGGGGACTCTAAAAATTATTAGACATAGTCAAGAAAAAATAATTTTACAG1450.10320137791632861No Hit
AATTAGGGGGAGGAAGATTCTAATTAATGCTTTCTCTCACTTTTGAATAGTC1440.10248964427552631No Hit
TTGGGGGATGCAGTGCTCGCTGTTGCTGAATAGGCGATCACAACGTGCATTG1440.10248964427552631No Hit
TGATTTGGGATTCCATAAAAATGGTAACCTAAAGAGAGCAAAGGTAGCTAGC1430.10177791063472406No Hit
AGCCTGGGCTACAGAGCAAAACTCTGTCTCAGAGTAAGAAAAGCTACCACAT1430.10177791063472406No Hit
TATTCTGGGAGTGGTTTTCATTGTATTCACAGAGTTGTGCAGCCATCACCAC1430.10177791063472406No Hit
TGCTCGGGGGAGCTGCAGACCGGAGCTGTTTCTATTTGGCCATCTTGGAATG1410.10035444335311952No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)