FastQCFastQC Report
Sat 31 Dec 2022
SRR5849318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140934
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGC9670.6861367732413753No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGCCGTCT9460.6712361814750166No Hit
AGTCAAGGGCAACAACTTCAGTAAAATGGCAGAAGAGAAACTGACCCACAAA2230.15823009351895212No Hit
TGTTCCGGGTTTCAGAGACATCTCAAATTGAAAGAATATCAGATTGCTTTTA1970.13978174180822228No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCCTGTCTCTTATAC1930.1369435338527254No Hit
GCTGAAGGGATACAAAACAACCAGAAAACAATTAACAAAATGACAAAAATAA1910.13552442987497693No Hit
CAGACTGGGCTCTAAGATGTGCATTGTGATCAATGACAGGAAAGTAAAAACA1830.12984801396398316No Hit
TTGAACGGATCAGTTGGATGATTCTCACCTTTACTCCTCAGTAAATCAGGAA1770.1255907020307378No Hit
TATCCCAGGGGGCCGCCATCGCGGGTGCGCGGGCGCAGAGCCTGGAATCCGG1730.12275249407524089No Hit
CACAGAGGGAGAATTTAAAATACCAGTAAACTAGATCTGATATAGAAGATGT1700.12062383810861822No Hit
TATTCTGGGAGTGGTTTTCATTGTATTCACAGAGTTGTGCAGCCATCACCAC1700.12062383810861822No Hit
TGAATAGCAGCACAAGGGACCATATTTTTAGTGCTTTTTCCCCTATGTCAAT1670.11849518214199553No Hit
TAGATCGGGATCTTAACTGGAAACAGAGTTTCTCAACCTTCAGATATTGAAA1670.11849518214199553No Hit
GTATTTGGGACCAGTTCTTAGAAGTATTGATCTCCAAGAAAGAGCAGAAAGA1660.11778563015312131No Hit
TAAACCGGGACTTCAGACAAGGATTCATGAATCAGGCAAATCAAACTATAAG1650.11707607816424709No Hit
TGATTAGGGTGCCTTCTGCAGGGAAGGAGCAGTCAGTGTTGTGGGTGTCCCT1630.11565697418649865No Hit
ATAATGGGGATGGTTCTTTATGAAAACAGTATTTTTTTATAAAACTGATCAA1630.11565697418649865No Hit
GCACTGGGGACAATTTAACCCATAACACACTGTATCCTGAAATAACTATCCA1620.11494742219762442No Hit
TGTTATGGGAAAGATGTACATTGAGGGAATATCAAAAAATAGCGTTCATGGC1590.11281876623100175No Hit
TAATGCGGGGGGAGTTATTTTCATTTTTTGAAATATATACAATGTGTGTGAT1590.11281876623100175No Hit
GTGCAAGGGATGAAATAGGCCCCCCAAAATAATACAACTTGTTTATGGGAAA1580.11210921424212753No Hit
TTCGGAGGGACAATTTAGGAATGCTCAATGTGCTGTTGATTCTTTCAATCCA1570.1113996622532533No Hit
GTCCACGGGCTGAATGTTTCATATTTTCTTGAAGCAGCATGTAATACTTTTA1570.1113996622532533No Hit
ATACGAGGGTAGCACGAGCTGAGTTGAAAGATAATTCAAAAATGTCTTCATA1570.1113996622532533No Hit
CATTCCGGGGTAATTCTAAATTTTCCCAGATAATACTTAGAATGAATTCCCA1560.11069011026437908No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTGTCTCTTATACA1560.11069011026437908No Hit
CTACATGGGCTCGGCTAGTATTATTTTAATAATTTGCTAATCACCTCAAAAT1540.10927100628663061No Hit
TCCCAAGGGATCATATCAACGTTTCTTCACGTCCATCCTTTTGGAGCCAACA1540.10927100628663061No Hit
CACTGGGCAAATAGCTCAGAAGTGAAATAATAGCATATAAAGAGAAAGAATA1530.10856145429775639No Hit
TAACAAGGGGCAGCCAACGTGGTGGGACCCAAAATCTGCCTGGAAGATAATG1520.10785190230888217No Hit
GTGATTGGGTAGAAAAACAAAAATAATAAAAGTGTTCCCATCCAGAGAGAAC1520.10785190230888217No Hit
AAACAAGGGATGTTTGCTTTCATATTTTATTATTACTTAGCTTTTCATCAGC1520.10785190230888217No Hit
AGCCTGGGCTACAGAGCAAAACTCTGTCTCAGAGTAAGAAAAGCTACCACAT1510.10714235032000795No Hit
TTGTTAGGGGGGAAGTTTCCTTAATAAAAGTGTGACGATTAAAGCTCTGGTG1500.10643279833113373No Hit
GGCAACGGGATATCTGAGAAATGGTTTAATTCATGCTCAGGAGCTGTGTGCC1490.10572324634225949No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCCTGTCTCT1490.10572324634225949No Hit
GTCGGCGGGGATCTTTCCGCGGGGATGGTGTGTTATGGGAAGACTTCTTAGA1480.10501369435338527No Hit
TGGAACCGGGTATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC1480.10501369435338527No Hit
GCTAAAGGGCTCCTGCCTCAGCCTCCCAAGTAGCTGGGACTACAGGCGCCCG1470.10430414236451105No Hit
GGAGAGGGGAGCCAACCAGATGGTGAAATGTGACCCTCGCCATGGTAAATAC1470.10430414236451105No Hit
TGTTCTGGGAGTCTTCCAGCTAGCAGGACAGACTATTTTTTTCTGACAAGAT1450.1028850383867626No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACA1450.1028850383867626No Hit
AGCCCTGTGACTTTTGATCACAAAATTTCTCATCTTGTTTCTTAAGTTGAGT1450.1028850383867626No Hit
ATTTGCGGGCTAGGTGCTTATCAGATATACGAATTGTAAATATTTTCTCTCA1450.1028850383867626No Hit
TTAGCAAGGGCCAACTCTCAGAATTCAGACTTCCTGCAGCTGCCATATCACC1440.10217548639788838No Hit
GGTGCAGGGGTCACCAATAGCCATGTGGTTAGTCCAGGGAACCAGTTTGTTA1440.10217548639788838No Hit
ACTTGCCGGGGTAGTTTCCACACTTCAAAAAATTGTACCAGATCAAAAATAT1440.10217548639788838No Hit
GGAGTAGGGAATCTTGTGTGGGAGCCATCTCAGGCTTCACTTGAACACAGGC1430.10146593440901416No Hit
TAAGACGGCACCATAGGAAGGTATCCAGAAGTCACATTTATGCAGTGTCTTC1430.10146593440901416No Hit
TCTTTGGGGGGCCCCTTTAACCTTTAGGGTGCGCGGGTGCGGTATATCTCGC1430.10146593440901416No Hit
GCTACTGGGTGAAATATTTTATAAGACATGGTGTTGAGGGAAGCACTGATGC1420.10075638242013991No Hit
CTAACTGGGATCATTGTACGCTGAGAAATTTTCATTCATTTTACAATAAGCA1420.10075638242013991No Hit
TGCTCGGGGGAGCTGCAGACCGGAGCTGTTTCTATTTGGCCATCTTGGAATG1420.10075638242013991No Hit
TTTGACGGGTCAAGTGATAAAACTTACTTCATAAAATAATTCATCTTAAAAT1410.1000468304312657No Hit
TACTTAGGGCATTGAAAATTTCTCGTTTTCATTTTCTTAGATGACTTCTTGT1410.1000468304312657No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)