FastQCFastQC Report
Sat 31 Dec 2022
SRR5849308.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849308.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71906
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGCCGTCT7861.0930937612994744No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGC7811.0861402386448975No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCCTGTCTCTTATAC1480.20582427057547356No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACA1360.1891358162044892No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCCTGTCTCT1100.1529774984006898No Hit
AAAAACGGGATGTCATGAGAAGGATGATTCATCTGAAGAACTAAGTGGAGAG1090.15158679386977442No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTGTCTCTTATACA950.13211693043695935No Hit
TACACAGGGAGCCCTAGAAGCATGAATTGCTTCAAATTATTGTCAACTTGTT930.12933552137512863No Hit
GAAATAGGGCATTTCCAGATGCAATAAACTAAAAGAATTTTTCAACACCAAA920.12794481684421327No Hit
AGTGACGGGATGGCTGCAAAATACTGTAACAAAGGTTGCTATTTAATTTGAA910.1265541123132979No Hit
GAGCTTGGGTCAGTATCCGTTTTTCAGTAATTGATCAAAGTAGATAAAGTAA880.12238199872055185No Hit
TACTTGGGGGGATGATAGCGATTGGGTAAATTAACTCTACATTGATATTCAA860.11960058965872111No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAA860.11960058965872111No Hit
CAAAAAGGGAATTTGCCCAAGGACAAAGAAAGTCAGATTATGGTTTTCAGGC850.11820988512780575No Hit
TTCACTGGGAGGGTACAGTGAACAATGATCACATCACTGCACTCCAGCCTGG810.1126470670041443No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGT810.1126470670041443No Hit
ATCCATGGGGGCAGTGGAGGCGGCCCAGGCCCGCCTTCCGCAGGGTGTCGCC800.11125636247322894No Hit
TTCTCGGGGACCTGCGAAGTGGTGCTGATGCACCTGGAGCAAGTGCCCATCT790.10986565794231357No Hit
TAAAGGGGGAACATCAATTGTGTATGTATGTGTGTTTGCGTTTGTGAATGTG790.10986565794231357No Hit
GCTCATGGGCCCTCAGAAGGAAATTTTCTTCACAATTTACTTCTTGAGCCTT780.1084749534113982No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTGTCTCTT770.10708424888048285No Hit
GGTTTGGGGGGAGATTAGTAGGATTTCTTTTCTAGTAGGTATGGGTGTGTCT760.10569354434956749No Hit
CTTCTCGGGGAAATGGGGATAGCTACAGCATTGAAATGTACCTAATACAATT760.10569354434956749No Hit
TTCAGAGGGTAGTTACATTAGATGTAAATGAATTAACTGCTTCTATCAAAAA750.10430283981865214No Hit
AGCTAGGGTAATGTTTAATCTGTTAAACCTGCTGCAAGAGCCATAACTTTGA750.10430283981865214No Hit
GAAGTAGGGTACTTAGCTGCTGAATATAAACAAAGTATACAGAAGCGTAAAT740.10291213528773678No Hit
TCTTTGGGCTGGAATGGTGGCTGGTCAATGTCTTTGCCCTCTCAAGTTGCTC740.10291213528773678No Hit
ACTGCTGGGAGAGGAACAAGGGTCCTTTGGTACATCTGATCATGAAATTTAA730.1015214307568214No Hit
AGCTACAGATTTCTCAAAAGAAACCCTACAAGCTAGAAGGGAATGGGGTCCT730.1015214307568214No Hit
GTTTAAGGGCTCACAAGCACATTCCAGGCCTCTGCCTGTGATAAGCTTGCCT720.10013072622590605No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)