FastQCFastQC Report
Sat 31 Dec 2022
SRR5849307.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849307.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72043
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGCCGTCT7921.0993434476632011No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGC7491.0396568715905778No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCCTGTCTCTTATAC1720.23874630429049315No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACA1390.19294032730452645No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCCTGTCTCT1090.1512985300445567No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTGTCTCTTATACA1050.1457462904098941No Hit
TACACAGGGAGCCCTAGAAGCATGAATTGCTTCAAATTATTGTCAACTTGTT1030.14297017059256276No Hit
ACTGCTGGGAGAGGAACAAGGGTCCTTTGGTACATCTGATCATGAAATTTAA980.13602987104923447No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTGTCTCTT940.13047763141457183No Hit
TTCTCGGGGACCTGCGAAGTGGTGCTGATGCACCTGGAGCAAGTGCCCATCT910.12631345168857488No Hit
AAAAACGGGATGTCATGAGAAGGATGATTCATCTGAAGAACTAAGTGGAGAG880.12214927196257791No Hit
GGTCTGGGGGGTTTCAGATGGAGATGAGGAACTTCTTGGGAACTGGAGCCAA840.11659703232791527No Hit
AGTGACGGGATGGCTGCAAAATACTGTAACAAAGGTTGCTATTTAATTTGAA830.11520897241924961No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAA830.11520897241924961No Hit
CTTCTCGGGGAAATGGGGATAGCTACAGCATTGAAATGTACCTAATACAATT820.11382091251058396No Hit
ATCAATCGGGAGGGTGGCAGAAGTGCTCAATGACCCTGAGAACATGGAGAAG810.1124328526019183No Hit
TTCCTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC790.10965673278458699No Hit
GTTTACGGGATTTAAATTATTATTTAAATTGTTCATCACAGTAGAAGTCATT790.10965673278458699No Hit
GAGCTTGGGTCAGTATCCGTTTTTCAGTAATTGATCAAAGTAGATAAAGTAA770.10688061296725566No Hit
GACGAGGGGGTGAACACATGAAAGATAAGAAAATGGAAAAGGCTTACTGATG750.10410449314992434No Hit
AGCTTTGGGCATACTGAATGGGGAAAAGATCTACAACAAGAAAGGATACCCA750.10410449314992434No Hit
CAAAAAGGGAATTTGCCCAAGGACAAAGAAAGTCAGATTATGGTTTTCAGGC730.10132837333259304No Hit
AGCTAGGGTAATGTTTAATCTGTTAAACCTGCTGCAAGAGCCATAACTTTGA730.10132837333259304No Hit
TATTGCGGGTATGGTCAGCATCTATTAATATGGATGAAAATTAATGGGAAAT730.10132837333259304No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)