FastQCFastQC Report
Sat 31 Dec 2022
SRR5849306.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849306.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71852
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGCCGTCT8311.1565440071257584No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGTATGC6800.9463898012581416No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCCTGTCTCTTATAC1650.22963870177587264No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACA1150.16005121638924455No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTGTCTCTTATACA1080.15030896843511662No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCCTGTCTCT970.13499972165005844No Hit
GTTTACGGGATTTAAATTATTATTTAAATTGTTCATCACAGTAGAAGTCATT880.1224739742804654No Hit
GCTCATGGGCCCTCAGAAGGAAATTTTCTTCACAATTTACTTCTTGAGCCTT860.11969047486500028No Hit
GAGCTTGGGTCAGTATCCGTTTTTCAGTAATTGATCAAAGTAGATAAAGTAA840.11690697544953516No Hit
TACACAGGGAGCCCTAGAAGCATGAATTGCTTCAAATTATTGTCAACTTGTT840.11690697544953516No Hit
AAAAACGGGATGTCATGAGAAGGATGATTCATCTGAAGAACTAAGTGGAGAG830.11551522574180259No Hit
AGCTAGGGTAATGTTTAATCTGTTAAACCTGCTGCAAGAGCCATAACTTTGA830.11551522574180259No Hit
CAAAAAGGGAATTTGCCCAAGGACAAAGAAAGTCAGATTATGGTTTTCAGGC820.11412347603407004No Hit
GAAATAGGGCATTTCCAGATGCAATAAACTAAAAGAATTTTTCAACACCAAA810.11273172632633748No Hit
GGTCTGGGGGGTTTCAGATGGAGATGAGGAACTTCTTGGGAACTGGAGCCAA810.11273172632633748No Hit
TACGTAGGGCATCATATGATTACATATTTATTTGCACATTCCTTTTTTTATT790.10994822691087235No Hit
TTCTCGGGGACCTGCGAAGTGGTGCTGATGCACCTGGAGCAAGTGCCCATCT770.10716472749540723No Hit
TAGTTGGGGTTATATAATTCATTGCGTAATGCAGGTGCTTGATTTCATCCGA770.10716472749540723No Hit
CAACATGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGTGG770.10716472749540723No Hit
GACGAGGGGGTGAACACATGAAAGATAAGAAAATGGAAAAGGCTTACTGATG760.10577297778767467No Hit
ATCAGTGGGGAGCAGGTAGTACCTTTACGGCTGTTGCTTTTTGCAGCATTAT760.10577297778767467No Hit
AGTGACGGGATGGCTGCAAAATACTGTAACAAAGGTTGCTATTTAATTTGAA760.10577297778767467No Hit
AGTCCGGGACTTATAAAAGAGCCTATATGATTAATCACTATTCTTGCTGCAC750.10438122807994211No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGTACAATCTCGT750.10438122807994211No Hit
AAACTTGGGGTTTGACAGATGAGGAGATGGAGGCCAGAGAGGATGGGCCACC750.10438122807994211No Hit
TACTTGGGGGGATGATAGCGATTGGGTAAATTAACTCTACATTGATATTCAA730.10159772866447697No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)