FastQCFastQC Report
Sat 31 Dec 2022
SRR5849290.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849290.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72424
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT3960.5467800729040098No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC3310.45703081851320004No Hit
TCAAAAGGGGATGTTGGAAACGGAAGCAGGCTTTTCAGTGTTTTTCTGGCAG870.12012592510769911No Hit
CGTGTTGGGTATGGGGAGGGAGGAGAATCTGATTTCCAGAGTTACCACATTA860.11874516734784049No Hit
TAGAGGGGTGACTCTTTGGTAATATATTGCTGATGTGAGTAGTAAGGGCATT830.11460289406826467No Hit
CAATGAGGGGGGTGGGCTGGGTCGGCGGCTGGAGCATTACCCCTACTGCGGG820.11322213630840605No Hit
CGCAATGGGAATGGAGGGTGGAAAGAGATTAAGTACAAAGTGAGGAAGATGG820.11322213630840605No Hit
CAAACCGGGATCGGGCAGGGCGCCGGCAGGGCTAATTTCCCGCTCGGCCCTG820.11322213630840605No Hit
TTCGGGGTAAGAGAGAGCAGACGTGACTAATATCAGACAAAATGATTTTAAA790.10907986302883023No Hit
ATGCTTGGGATAGCAAAACTTAATTTTAGCATTCTCCTGCCCACAGTTTGGA780.10769910526897161No Hit
TGTCAAGGGATATTTCAAAAAGTATATTAGAAATTATACATTTACCCATAAT750.10355683198939579No Hit
GCTCTAGGGGTTGCTGCTTTCCATTAACTAGTCTTGAAATGCCTTTGTTTGT750.10355683198939579No Hit
ACTAGCGGGCAAAAGGTTCAAACAATTTTTAAGTAATTTGGCTGTGTCCCAG750.10355683198939579No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)