FastQCFastQC Report
Sat 31 Dec 2022
SRR5849289.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849289.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71697
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT3870.5397715385580987No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC3640.5076920931140774No Hit
CGCAATGGGAATGGAGGGTGGAAAGAGATTAAGTACAAAGTGAGGAAGATGG940.13110729877121777No Hit
TCAAAAGGGGATGTTGGAAACGGAAGCAGGCTTTTCAGTGTTTTTCTGGCAG920.12831778177608547No Hit
TGCCTTGGGTACAAAAAATAGAGGAAGAGGGAATACTTTCAAAGTCATTCCA890.12413350628338704No Hit
CGTGTTGGGTATGGGGAGGGAGGAGAATCTGATTTCCAGAGTTACCACATTA790.11018592130772556No Hit
CTCATGGCGGGAGAAAAAGCAAAGAGAACCAGTAAAGGTAAAGATAATTATA780.10879116281015942No Hit
AGCAGTGGGGTCTGCAAAAACGAATCTTTTCCTACAACCTGTTAACTGACTG780.10879116281015942No Hit
TATTGCCGGGGTGGACGAACGGGAGCAGATGGCCATCAGTGGCGGCTTCATC760.10600164581502712No Hit
TTAAACGGGGGCAGACGGCGGAGAGCAGAGAGGGAGCGCGCCTTGGCTCGCT760.10600164581502712No Hit
ACTGGGGGGGGTGCCTCGTGGCTCATTCCCCAGACCTCTTTCCTCAGTAATG750.104606887317461No Hit
TGCCGATGGGAAGTTAAATTCTCTTATTTAAAATTTTTTTTATTTTTATAAA740.10321212881989485No Hit
TAATATGGGTTTCCACCTTAATTAGATGATAGAAATAGTACTTGTTATCTTG730.1018173703223287No Hit
CAAACCGGGATCGGGCAGGGCGCCGGCAGGGCTAATTTCCCGCTCGGCCCTG730.1018173703223287No Hit
AGTCAACGGGGAGGCATTGAGGCAGCCAGCGCAGGTGCTTCTGCTGAGGGGG730.1018173703223287No Hit
GTAAATGGGGGACGCGGAAGACTATAAGCCCCAGCGGGCGACGACCGAACGC730.1018173703223287No Hit
TTACATGGGGCAATTTTTGAAGGCACAAAGTCATTTTAAAATGGACTTTTAG720.10042261182476254No Hit
GAAAAAGGGGAAGCAGCAAAGTGGGTCGGAGCTGGAAGAACTCATGAACAAG720.10042261182476254No Hit
TATGCCGGGATGCGGCTGCGGGGCCCGCGGCCCGGAGCGTCCGCCCAGCCCT720.10042261182476254No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)