FastQCFastQC Report
Sat 31 Dec 2022
SRR5849288.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849288.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71524
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT3960.5536603098260724No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC3690.515910743247022No Hit
CGCAATGGGAATGGAGGGTGGAAAGAGATTAAGTACAAAGTGAGGAAGATGG880.12303562440579385No Hit
TCAAAAGGGGATGTTGGAAACGGAAGCAGGCTTTTCAGTGTTTTTCTGGCAG850.1188412281192327No Hit
ATAAGAGGGCATCGTGCCCCTTTTATAAAAATTCATGCATGGAATCCTACAT740.10346177506850847No Hit
AAGTACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA720.1006655108774677No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)