FastQCFastQC Report
Sat 31 Dec 2022
SRR5849284.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849284.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83680
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT6200.7409177820267686No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC4570.5461281070745697No Hit
GAGTTAGGGAGCTCAAGGAACTAGATAAGCAAGAAAAAAACAAATCCAAAAT1360.16252390057361377No Hit
ACTTCGGGATGTTTTGGCATAATAGGGTTTTAGTCATGTAAGGGATATGTCA1270.15176864244741875No Hit
GCTGCCAGGTCTTTTTAATAAAGATTAATAAATTTTACTTCATACTACATAT1250.14937858508604207No Hit
AAATTAGGGATAGTAGAAATTAAAACATCAACAAATGGATTAGCAAAATAAT1210.1445984703632887No Hit
ATTTCCGGGACATAAAAAGAATTTTGTGTAACTGGCAGTTTGAGCAACAATG1160.13862332695984705No Hit
TTAAGGGGATGCTGGAAATGGAAGCAGGCTTTTCAGTGCTTTGTGGCAGTCT1160.13862332695984705No Hit
CAAAGGGAGTCATGAATCAGTTGACAACAAATTTATTGCACAGAATATTTCT1160.13862332695984705No Hit
TGCTGTGGGATAACAAAAATCATGTGAAAACCAAAATTAAAAACATAATAGT1150.1374282982791587No Hit
ACTTTGGGAGCACACAATTAACCAACTGGAAGAATTCAAGTGAATGTCTTTC1150.1374282982791587No Hit
CATTACGGGAACTACACAAAACTGACCAATCTGGGTTGCCAAAGCAGCCAGG1130.13503824091778202No Hit
CATGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1130.13503824091778202No Hit
GTCTTCGGGTATAATTTTTAGGTAGATGTTTGGTTGTTGTTTTGATCAAATT1090.1302581261950287No Hit
TTAGTCGGGAATTTGGTCTTTATTTCGAAGGTGGTAGAAAGCCCTAACTTCT1090.1302581261950287No Hit
TGTGCTGGGGGGCCCCGGATGCTGCGGCGCCCGCTGGCCGGGCTGGCTGCGG1090.1302581261950287No Hit
GGCACGGGGGACGCTCTTCCGATCTGGGCATGGGCTCTTTCCCTTCGGTGTG1090.1302581261950287No Hit
TGTCCAGGGATTTTCATGAGTCAGCAAAAGCCCTACGCTTGATTCCAACAGA1080.12906309751434034No Hit
TGCGTAGGGATGGAAAATATTCACAATTTAAGGAAGCTATTTAATTTTTATT1080.12906309751434034No Hit
CAGCAAGGGGTGCTGAGAGGAGAGTCATGAGTCTGAGAAGGGCACCATTTCA1070.127868068833652No Hit
GAATCCGGGACATTGATAAATTTTTAGCATTTTAAGCATATCGGAATTATTG1070.127868068833652No Hit
CAAACAGGGGTCTTACTCTTAAACCTGGTAACACTTGATTTGCCTTCTATAA1060.12667304015296368No Hit
CACCTGGGGCAAATATTTATTTTTGTAAGCCTAAAAGTGATTCTTTGAAAGT1060.12667304015296368No Hit
ATGCGTGGGACACATATGCAGTAACATGAAAATAAGATGTCAGCACCCATCC1050.12547801147227533No Hit
AAGCATGGGATAGTAAGTGGGAATACATACATGGATAAACATTTATTCCAGA1030.12308795411089866No Hit
CGTTATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1020.12189292543021034No Hit
ACTACTGGGGCAGAAGGCAAGAAATAATTAAGATCAGAGCAGAACTGAAGGA1020.12189292543021034No Hit
TGTCCAGGGTAAGCAGAATAAACAAAAACCAGAATCTCTTGAGAATAGTAAC1010.12069789674952199No Hit
GACGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1010.12069789674952199No Hit
TGTTCAGGGAGGCCATTGATATCGCAGTTCTTCCTATCAGCTTCAGTGACCC1010.12069789674952199No Hit
CAGCTGGGGGTACATTTCAGTTATTTTTTTTCAAGTTCTTCAGATTGTGACC1000.11950286806883366No Hit
CATAACGGGTGCTAACAGGCTTCAGATGTGTCTAATCACAAGAAACACTTGT1000.11950286806883366No Hit
TAAATGGGGGGGGCAAGTGACTTGTATGCCACTGAAGCAATTTCTTGCTTTA1000.11950286806883366No Hit
CTGCAGGGGGAGGATTTCAGTGTTTTGTCTGGGTTGTTTCTCCCTTTACACA990.11830783938814532No Hit
ATTACAGGGCTGAGAGATAGTTGTTAAAATTCCAATTTACATTGTGGATTTT990.11830783938814532No Hit
TGCCAAGGGGCCATGGCATAAGGAACTCTATCCCAGGAATACAGCTTTGCAT990.11830783938814532No Hit
CGTATGGGGATTGATGATGCAAACTACTCCGTTGGCCTGTTGGATGAAGGAA990.11830783938814532No Hit
TCTACCGGGGGGTGAAGTGGATTTCTGGATTTAGCGATGTGAAACTAGATTA990.11830783938814532No Hit
GAAATTGGGTAATTTAGTTTTTCTTATTTTATCTACAACAATGGCTCTTAAA990.11830783938814532No Hit
GTCCCAGGGAAGGAACTGTATTTTTGCACAGTGATATATCTTCAACATATTA990.11830783938814532No Hit
ATGCTGGGGGCGTCGCTTTCAGCCTCTGGTGAAGGGCGGCGCGCTTAGGCAG980.11711281070745697No Hit
ATCACCTGGGAGAAATGAGATAAATGGCAACACGATAATAGTGGGGGACTTC980.11711281070745697No Hit
GTAAGTGGGACTTGCATTAATGGCAACTCTTGCAGTTGCTCTGCTAGAAACT980.11711281070745697No Hit
CTTACAGGGCACAGAAACCAAGATGGAAGAATTCAAGGACCAATTACCTGCT980.11711281070745697No Hit
TTAGGCCGGGAGAAGGAAGATGGCGGATCTGGAGGAGCAGTTGTCTGATGAA980.11711281070745697No Hit
CAGCCGGGACAAAGAAAGCACGAAGTTACTTTAGAATAGAAAATAAAATCTT980.11711281070745697No Hit
AGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGAT980.11711281070745697No Hit
GTGCATGGGCTGAAGAAAATGTTTGAAGCAACAAGATTGCTTGCAACAATTG960.11472275334608031No Hit
TCTTTGGGGGAGGTTATGGATACCCTAAATACTCTGACTTTATCATTATACA960.11472275334608031No Hit
ATCCTCGGGACCTGATAAATATGTATCTAGTTCTACAAGGTCACATTTATGT960.11472275334608031No Hit
ATCACAGGGGACTGTGATAATTTAAAGATGTATATTATAACCATAGAATAAT960.11472275334608031No Hit
GCTTGAGGGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGC960.11472275334608031No Hit
AAGAATGGGAAGCATTGTGCATGATGTCTTTCTTTAGAAAGCTTGATTATGT950.11352772466539197No Hit
TAACGCGGGAAAGCCTGCGCCTCATGTGCCTTTTGTCCATCACTGAGAATGG950.11352772466539197No Hit
TGTAGGGGAGGGCCGGCGAGCGCAGTGCAGCGCCGTGCGGTGCGGGCGGCCG950.11352772466539197No Hit
TTGTCTGGGTTGAAGAAAAGGTTTGCCTGTGAAAGAGATCGAAGGCCTACTG950.11352772466539197No Hit
GAACTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA950.11352772466539197No Hit
TTGCAGGGGGTAGTGGTAATAAACTTAAATACTATAGTACTTTTCTTATGTA940.11233269598470363No Hit
CACTAGGTGGCCAGGTAAACTTACACTAATGGTCTGACTTTGCTGTGCCTCA940.11233269598470363No Hit
AAACCAGGGAGCATATATATGTAAATAACTCTCATATCTGCATTTATTTTAA940.11233269598470363No Hit
ACCTAAGGGATGAACCATTACTGAAACGTCTTGGTGCTGCCAAGCATTAGGT940.11233269598470363No Hit
TACAACGGGGGAGGCCGGGTTGGCGCCGGAGCCGGGGCCGCTTGGAGCTCGT940.11233269598470363No Hit
CTCGTGTCGGGAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAG930.1111376673040153No Hit
CAATATGGGTGCCATGGAAAAATGCAAGTTATAGCATATTTCATTTTTATAA930.1111376673040153No Hit
CTCGCTGGGTCAGGAGCTCAGTTTCTTTTTCACTTGGGTCTCTGGATTCCTG930.1111376673040153No Hit
TTTTAAGGGAAGCTTCCAGAGGAAGGATCAGGCAGCAACATTTGCCGTTCTG920.10994263862332697No Hit
AAAGTCGGGGGCACAAATGCAGAATTCATGACCTTGTAGTTGTGGCAGGGTC920.10994263862332697No Hit
GACTTTGGGGGAAACTCTGTTGTAGAAATATTGGCATCCGTTCAAATTCTAT920.10994263862332697No Hit
TGACAGCGGGGTCCTTCAGAAAGCTTGATCAGTTTAAAATTGCTTTCTAAAT920.10994263862332697No Hit
TAATTGGGGCTTGAAGAGAGAAAGCAGAAGGAGCAGAATCGGTTGGAGAAAA920.10994263862332697No Hit
GTAGTTGGGGTCTAGTGACACAGGAGTGTCCAAAAAGAAAAATAGTAGTGAC920.10994263862332697No Hit
CTTCTAGGAGAACCTGATGGTGGGAAAGAATTGGCTACATTGATGGAGCAAA920.10994263862332697No Hit
GCTACTGGGATGAGGAAAACTCTCCAAAACATTGGTTTGGGCAAAACTTCTT910.10874760994263862No Hit
TTTAGGGGCATAAATGTACCCAGTAACCAGGTTTTCTAGAACACATTTATTT910.10874760994263862No Hit
TAAGGCAGGGAGTGCCAGCTCATTGCAGGCCCTGATTAGATATTTATTGAAT910.10874760994263862No Hit
TACACTGGGGTTTGTGAGGATTGTACCTGTTGAGAGAAGTTGCAAAAAGAAT900.10755258126195029No Hit
CTTGTGGGGCTTATCAAGCGTAACACGACCGTGCCAACCAAGAAGTCGGAGG900.10755258126195029No Hit
AATCTAGGGGCTAAACCTCCTGCAAAAGTGGAAGCGAAGCCGAAAAAGGCAG900.10755258126195029No Hit
AAACTCGGGTATTGCACCTTGGAATTTCCTGTGGAGGTGAACACAGTACTAC900.10755258126195029No Hit
AATGGCGGGTAACCTTTTTCTTCTGGTCTTTGCAAATCCTGCCGTAGAAACT900.10755258126195029No Hit
CTCCGGGTGAACTGATTTAGTCCTAATTCCAAGAAGTGTGATTCCACCTACT900.10755258126195029No Hit
CGTATCGGGTACCCCTGGGGGAGAGAAATCTATACTTTAGAAGGTGTTGTGG900.10755258126195029No Hit
ATCGTTGGGATTGTATGAAATGCTAGAAAGATTAGAGTAAGAGAAATTTTAC890.10635755258126195No Hit
TCGACTGGGCTGACAGAAATTGAGGCAGCTGTTTGCTTATGTATTTGTTTTT890.10635755258126195No Hit
ATAGTAGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC890.10635755258126195No Hit
ATCGTGGGGAGCCCCACACGGACCAGTGTGCAGAAGGCTCCGGGAACCGCAG890.10635755258126195No Hit
GCTGGCGGGGTGGCTTCTAAGTAAGTCCTGCAGTCTTATTTCTCACTTCTGT890.10635755258126195No Hit
TCTTGCGGGCATATGGTTCCAAGTTGATTAAGCTTTGTTACCAGCAGACTAT890.10635755258126195No Hit
CCGCAGGGGGGATTACAGGTGTGAGCCACCACGCCTGGCCAAGAGCCTCACT890.10635755258126195No Hit
TCAAATGGGGCTCCTGCCAGTAACAGGGAAGCTGACACGGTGGAACTGGCTG880.10516252390057361No Hit
TTAGCTGGGCAGCCAACCCCTTGCAGAGTGGCAGCTTTGCAAGGCATCCGTG880.10516252390057361No Hit
ATGCATGGGACCACGTAAATCAAGCCATTCATTTTGGGGAACAGAAGATCCA880.10516252390057361No Hit
ATGGCGGCGGGATCTACTTGCGGGTCCATCAGTGCCAGCATACCAATGAACC880.10516252390057361No Hit
GGTCACGGGATATGGAGTGTATTTTCTCCAAAACTTCTCTACGAGGGAATGC870.10396749521988527No Hit
CTACATGGGCATGACTTACTTTATAAATAATGTTTACATGCCATAAGTCCTT870.10396749521988527No Hit
CTTCAGGGGAGGATGATGGAGGGGATGAGAGGAGGTTCAATTCTAAGTGTAC870.10396749521988527No Hit
CGAAGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA870.10396749521988527No Hit
CGTTATGGGCTGGCTAGGCAAATAAGCTTTAAGGTACTATAAATTCTTAAAT870.10396749521988527No Hit
ATTTGGGGGAACTTTTAGAACTTTTTATCTGCTGTAATTTCTTTTTACTTTT870.10396749521988527No Hit
ACCGCTCAGGGTGGACATCATCACCAAATTCCTGAAGTTTCTTCTTGGCGTT870.10396749521988527No Hit
GTTGAAGGGCGAGATTGCGGGCATCGAGAACGACGAGGCCTTCGCCATCCTG870.10396749521988527No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCCTGTCTCTTATAC860.10277246653919694No Hit
CTATTGGGGGCAACTTAGAGCCAAGACTGGTGGCCACCTCCATTTATTTCCC860.10277246653919694No Hit
GACCTGGGGATCTTTAGCCCTCTGAGTACTTTGAAGCACTTTTGCATTAATT860.10277246653919694No Hit
TGCAGGGCACAACAAAAAACGTTGGTTTGAAATTTGACAAAATGTTCTGCAT860.10277246653919694No Hit
ATAAACGGGCGATCTTCGCATGGGCATTTACCTCTTATCTCCACCTTGAAGA860.10277246653919694No Hit
CAACTGATCCACCCACCCTGGCCTCCCAAAGTGTCAGGATTACAGGCGTGAG850.10157743785850862No Hit
CGAGTGGGGTACTAATTTACTATATTAGTCAGTTTTCACACTGCTGATAAAG850.10157743785850862No Hit
TACAGCGGGAGGTCAGGAACCCTGTACAGGGACTGTGTAGGCAAAGGTATGA850.10157743785850862No Hit
TAGGGGGATACACACACAGCATACTTTTCAAGTTAATTCAGCACACCTTAAG850.10157743785850862No Hit
AAGTTAGGGGACCGCCATGGCCACGGAGGACCCCCAGAGCGCCACGGCCGGG850.10157743785850862No Hit
ATGTTTGGGGATGCAGGAAGGACTCTCCAATCTTCCCCTGAAAAAGGTCATA850.10157743785850862No Hit
AAATATGGGAACAAACTGAAGAATTGTCTGAAAGAAAATATGGCATGTCTAA850.10157743785850862No Hit
TACTTAGGGTCAGTTTTACTAAGCAAATCAGAAACTTAATAACAATGGCATA850.10157743785850862No Hit
TCTGTCGGGAGATCCTGTGAAGATAATGACCAGGGCTTTCCCTGTGTATATA850.10157743785850862No Hit
ACAACGGGGGAAAATATCTAAATAGATATCCATATCACATGGAAACGATGTA850.10157743785850862No Hit
ATCGCTGGGATTCTCTTGTCTATCTGTGATCCTCAGGGATGCAATCACAGGC850.10157743785850862No Hit
CAAGTTGGGGCGTATTCATATAATGCAACACTACTCTATAATTTTTTAAAAG850.10157743785850862No Hit
TCTTGTCGGGGTGGTCAAAAAAGATTCTAGATATGGTCTCAATATTATTAAA840.10038240917782026No Hit
TAACGTGGGAACCAATGTTTGAAAATTCGTTTTAGCCAATCAACCAATTGGT840.10038240917782026No Hit
AACATTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA840.10038240917782026No Hit
ATGAGCGGGAATAGCACACAACTCAGAACTCTTCAGCATTTGTGTGATTCCT840.10038240917782026No Hit
AGGTAAGGGGGACCGGATGAAGCAGGAGATCCTAGAGGAGGTGGTGAGAGAG840.10038240917782026No Hit
TGTAACGGGCAGACACCCGCTGATCAGAGACATGCTTCGACGAATTAAGGAA840.10038240917782026No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)