FastQCFastQC Report
Sat 31 Dec 2022
SRR5849283.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849283.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83537
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT5600.670361636161222No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC5140.6152962160479787No Hit
TGTCCAGGGATTTTCATGAGTCAGCAAAAGCCCTACGCTTGATTCCAACAGA1450.173575780791745No Hit
CTTACAGGGCACAGAAACCAAGATGGAAGAATTCAAGGACCAATTACCTGCT1370.1639991859894418No Hit
TTAAGGGGATGCTGGAAATGGAAGCAGGCTTTTCAGTGCTTTGTGGCAGTCT1330.15921088858829022No Hit
GAATCCGGGACATTGATAAATTTTTAGCATTTTAAGCATATCGGAATTATTG1280.15322551683685073No Hit
GCTGCCAGGTCTTTTTAATAAAGATTAATAAATTTTACTTCATACTACATAT1270.15202844248656283No Hit
GGCACGGGGGACGCTCTTCCGATCTGGGCATGGGCTCTTTCCCTTCGGTGTG1240.14843721943569915No Hit
TGTAGGGGAGGGCCGGCGAGCGCAGTGCAGCGCCGTGCGGTGCGGGCGGCCG1200.14364892203454757No Hit
TTAGTCGGGAATTTGGTCTTTATTTCGAAGGTGGTAGAAAGCCCTAACTTCT1190.14245184768425967No Hit
CAAAGGGAGTCATGAATCAGTTGACAACAAATTTATTGCACAGAATATTTCT1190.14245184768425967No Hit
TACACTGGGGTTTGTGAGGATTGTACCTGTTGAGAGAAGTTGCAAAAAGAAT1170.14005769898368386No Hit
TTGTCTGGGTTGAAGAAAAGGTTTGCCTGTGAAAGAGATCGAAGGCCTACTG1160.138860624633396No Hit
GTCTTCGGGTATAATTTTTAGGTAGATGTTTGGTTGTTGTTTTGATCAAATT1150.1376635502831081No Hit
GAGTTAGGGAGCTCAAGGAACTAGATAAGCAAGAAAAAAACAAATCCAAAAT1150.1376635502831081No Hit
CACCTGGGGCAAATATTTATTTTTGTAAGCCTAAAAGTGATTCTTTGAAAGT1140.13646647593282019No Hit
AAAGTCGGGGGCACAAATGCAGAATTCATGACCTTGTAGTTGTGGCAGGGTC1120.13407232723224438No Hit
ATAGTAGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC1120.13407232723224438No Hit
CATGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1080.1292840298310928No Hit
TGCTGTGGGATAACAAAAATCATGTGAAAACCAAAATTAAAAACATAATAGT1070.12808695548080493No Hit
ACTTCGGGATGTTTTGGCATAATAGGGTTTTAGTCATGTAAGGGATATGTCA1070.12808695548080493No Hit
CGAAGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1070.12808695548080493No Hit
AATCTAGGGGCTAAACCTCCTGCAAAAGTGGAAGCGAAGCCGAAAAAGGCAG1060.12688988113051702No Hit
GACGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1050.12569280678022912No Hit
TGTTCAGGGAGGCCATTGATATCGCAGTTCTTCCTATCAGCTTCAGTGACCC1050.12569280678022912No Hit
TGCCAAGGGGCCATGGCATAAGGAACTCTATCCCAGGAATACAGCTTTGCAT1050.12569280678022912No Hit
CATAACGGGTGCTAACAGGCTTCAGATGTGTCTAATCACAAGAAACACTTGT1050.12569280678022912No Hit
CGTATGGGGATTGATGATGCAAACTACTCCGTTGGCCTGTTGGATGAAGGAA1040.12449573242994123No Hit
TTAGTGCGGGCAGCTAAGCCATGCCTGGATTCCTTTTCCTGGAAACTATGGA1030.12329865807965332No Hit
ACTTTGGGAGCACACAATTAACCAACTGGAAGAATTCAAGTGAATGTCTTTC1030.12329865807965332No Hit
TGTGAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1020.12210158372936543No Hit
AACATTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1020.12210158372936543No Hit
CTCGCTGGGTCAGGAGCTCAGTTTCTTTTTCACTTGGGTCTCTGGATTCCTG1020.12210158372936543No Hit
ACCTAAGGGATGAACCATTACTGAAACGTCTTGGTGCTGCCAAGCATTAGGT1020.12210158372936543No Hit
ATTCTCGGGCACTCTAATGACTTTGATGAATGTCCTTAAATATGTGTGCACC1010.12090450937907754No Hit
ATCGTTGGGATTGTATGAAATGCTAGAAAGATTAGAGTAAGAGAAATTTTAC1010.12090450937907754No Hit
TACTTAGGGTCAGTTTTACTAAGCAAATCAGAAACTTAATAACAATGGCATA1010.12090450937907754No Hit
TAAGTAGGGTGAATCAAAATGTGGTAGTTTGGTATTTCAGATAGCTAACTTT1010.12090450937907754No Hit
CTTGTGGGGCTTATCAAGCGTAACACGACCGTGCCAACCAAGAAGTCGGAGG1000.11970743502878964No Hit
CATTACGGGAACTACACAAAACTGACCAATCTGGGTTGCCAAAGCAGCCAGG1000.11970743502878964No Hit
CTGCAGGGGGAGGATTTCAGTGTTTTGTCTGGGTTGTTTCTCCCTTTACACA1000.11970743502878964No Hit
CGACCGGGGATACTATACGTGGTCTTTTGTAGCTGGCTTCTTTCATTTAGCA1000.11970743502878964No Hit
TAGCATGGGATCCTGGATGATGCCACCAGTGCCCTGGATGCAAACAGCCAGT1000.11970743502878964No Hit
CGTAGCGGTATGCAGAAACTGTGATGACTTTGATTTTTGTGAAACGTGTTTC1000.11970743502878964No Hit
GCTACTGGGATGAGGAAAACTCTCCAAAACATTGGTTTGGGCAAAACTTCTT990.11851036067850175No Hit
TTTTAAGGGAAGCTTCCAGAGGAAGGATCAGGCAGCAACATTTGCCGTTCTG990.11851036067850175No Hit
TAATTGGGGCTTGAAGAGAGAAAGCAGAAGGAGCAGAATCGGTTGGAGAAAA990.11851036067850175No Hit
ATGCATGGGACCACGTAAATCAAGCCATTCATTTTGGGGAACAGAAGATCCA990.11851036067850175No Hit
CGTTATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA980.11731328632821383No Hit
AAGTTAGGGGACCGCCATGGCCACGGAGGACCCCCAGAGCGCCACGGCCGGG980.11731328632821383No Hit
ACGCAGGGGCCGTGGCACAGGAAGGCCAATGTCGGGTAGATGACAAGGTGAA980.11731328632821383No Hit
AGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGAT980.11731328632821383No Hit
ATTTCCGGGACATAAAAAGAATTTTGTGTAACTGGCAGTTTGAGCAACAATG970.11611621197792595No Hit
TGATTTGGGTCAAGATATTCTCTTTAGACAAAGAGAAGAGAAAACATTTAGT970.11611621197792595No Hit
AAATATGGGAACAAACTGAAGAATTGTCTGAAAGAAAATATGGCATGTCTAA970.11611621197792595No Hit
CAGCTGGGGGTACATTTCAGTTATTTTTTTTCAAGTTCTTCAGATTGTGACC970.11611621197792595No Hit
TAAATGGGGGGGGCAAGTGACTTGTATGCCACTGAAGCAATTTCTTGCTTTA970.11611621197792595No Hit
TGTCCAGGGTAAGCAGAATAAACAAAAACCAGAATCTCTTGAGAATAGTAAC960.11491913762763804No Hit
CGTGGTGGGGTCTCAGAGGATTACCTGGCTGTGTGACGTGTCAGTCTGCCCC960.11491913762763804No Hit
GCTGGCGGGGTGGCTTCTAAGTAAGTCCTGCAGTCTTATTTCTCACTTCTGT960.11491913762763804No Hit
CACAGGGATCCTTGAAATTCTACGTTTCTGATTTTAACATCTAAAATTTTAA950.11372206327735015No Hit
TTTCACGGGAACTCTTTTTTTCCAGTGTTTCAGGAAGTTTCTCTTCCTCCCA950.11372206327735015No Hit
ACTACTGGGGCAGAAGGCAAGAAATAATTAAGATCAGAGCAGAACTGAAGGA950.11372206327735015No Hit
ATTTTTGGGATATTTAATACTTTTCTCATATGTAAGTTATGCATGTCTAGAG950.11372206327735015No Hit
AAGAATGGGAAGCATTGTGCATGATGTCTTTCTTTAGAAAGCTTGATTATGT940.11252498892706227No Hit
TAACGCGGGAAAGCCTGCGCCTCATGTGCCTTTTGTCCATCACTGAGAATGG940.11252498892706227No Hit
TGTGCTGGGGGGCCCCGGATGCTGCGGCGCCCGCTGGCCGGGCTGGCTGCGG940.11252498892706227No Hit
CCGCTAGGGGATCCTTGCCCTACTCAATTCCTTGATATCATCTGCCTGGTAA940.11252498892706227No Hit
ATCAGCTGGGGAAGCAAATGTAACATCAAGATAGGAAGAAGTGGGTGTATTC940.11252498892706227No Hit
GGAGCCGGGGGAGCCAACTCAGGCCATTTCCCCTTGCTGTGGTTTCTTACTG940.11252498892706227No Hit
ACACCAGGGGAAATAAATATCCATGAGTTTGTAGGGTTAAAATTAATAAATA930.11132791457677436No Hit
GACTTTGGGGGAAACTCTGTTGTAGAAATATTGGCATCCGTTCAAATTCTAT930.11132791457677436No Hit
GTCATGGGGCAATATGTTAAGAGCCTTGGCTTTGGTAGCCTCCTGGCAGGAG930.11132791457677436No Hit
AAGCATGGGATAGTAAGTGGGAATACATACATGGATAAACATTTATTCCAGA930.11132791457677436No Hit
AGTTGTGGGTGAATGACCGAGTCTGTGCAAAGGGACTCTGTATGCTTTCCTT930.11132791457677436No Hit
TCTGTCGGGAGATCCTGTGAAGATAATGACCAGGGCTTTCCCTGTGTATATA930.11132791457677436No Hit
TACAACGGGGGAGGCCGGGTTGGCGCCGGAGCCGGGGCCGCTTGGAGCTCGT930.11132791457677436No Hit
ATTTAGGGAGTTTAAGGCATTGATAACTTACAAGTAGAATGGGGCTCTCAAA930.11132791457677436No Hit
GTTGCTGGGATCCTTGGGGCAGAACAGTATCCTTTATGGGGCAGATTTGTTA920.11013084022648648No Hit
TTAGGTAGGGAACATTCATCTTGTACATCTCCATCAGAGCTCATGAGTGACT920.11013084022648648No Hit
TAACGTGGGAACCAATGTTTGAAAATTCGTTTTAGCCAATCAACCAATTGGT920.11013084022648648No Hit
GCGCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC920.11013084022648648No Hit
TTTCTAGGGCATGCCGGGAGTGATCAGTCGGACATTTTAATTTGAACCACGT920.11013084022648648No Hit
CTTCGTGGGAAAACTTCTGAGTGACCACCACATCTACCTGGAAGGCACCTTG920.11013084022648648No Hit
TGCATAGGGCAGTAAGGCAGAATTTGTCTTTCCCAGATTCAATCCATATGGA910.10893376587619856No Hit
GAAACGGGGGACCTTTGCTTATGCAAATCAGAAAAACAGAATGGACTAGGAC910.10893376587619856No Hit
ACTTCAGGGCGATCTGGCAATGGGGGTAACTCCTGAACCAGATCTTTGGCTG910.10893376587619856No Hit
AATTCTGGGTTCTAAGTGATATGGTACACATTTCTGCTTCCAAAATGGGATA910.10893376587619856No Hit
TTACGTGGGTAGAGTCATTTCATTTAACACAGGAACTGAGACTTTTGAAGGA910.10893376587619856No Hit
GATGGTGGGTTACATGTGCCATTTTTTTAATTCGAGTAACCCATATTTGTTT910.10893376587619856No Hit
TGCGTAGGGATGGAAAATATTCACAATTTAAGGAAGCTATTTAATTTTTATT910.10893376587619856No Hit
GCGACTGGGAATTTAAATGAACTAGTTAAACATGGTCTGCGTGCCTTAAGAG910.10893376587619856No Hit
TAAGGCAGGGAGTGCCAGCTCATTGCAGGCCCTGATTAGATATTTATTGAAT910.10893376587619856No Hit
ATGCTGGGGGCGTCGCTTTCAGCCTCTGGTGAAGGGCGGCGCGCTTAGGCAG900.10773669152591067No Hit
CGATGGGGGGAGGAGAAAGATGGCGGCTACCTTACTAGCTGCTCGGGGAGCC900.10773669152591067No Hit
CGTTATGGGCTGGCTAGGCAAATAAGCTTTAAGGTACTATAAATTCTTAAAT900.10773669152591067No Hit
TATCCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA890.10653961717562278No Hit
CTACATGGGCATGACTTACTTTATAAATAATGTTTACATGCCATAAGTCCTT890.10653961717562278No Hit
TACTTGGGACAATAGACAAATAAAAATGATCACTCTGTCTTTTGATTGGAGA890.10653961717562278No Hit
GACAGTGGGCAATTTTTACACTGTTATAAAGAAGATGAACTTATACATAATT890.10653961717562278No Hit
AGTATGGGGACTTTCTGGGGTGGTATTGTATGTCATGTTTACCAATTGTATT890.10653961717562278No Hit
CAGAACGGATTGTGGGTTCAAAGCATTCTCTGCAAATAGGCATCTCAGCTCC890.10653961717562278No Hit
GTCCCAGGGAAGGAACTGTATTTTTGCACAGTGATATATCTTCAACATATTA890.10653961717562278No Hit
CTCGTGTCGGGAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAG880.10534254282533488No Hit
TGCAGGGCACAACAAAAAACGTTGGTTTGAAATTTGACAAAATGTTCTGCAT880.10534254282533488No Hit
GGCGCAGGGTAAGTGATTCAAAATGAAAATATTTACTTTGTAGAATATGACT880.10534254282533488No Hit
TCTTGCGGGCATATGGTTCCAAGTTGATTAAGCTTTGTTACCAGCAGACTAT880.10534254282533488No Hit
AACCTTGGGAACGTAGAAGCCTTGCATCCTTTTCTTGTGTAAAGTATTTATT880.10534254282533488No Hit
ATACTGGGCAGCCTTAAAAATTTTCTTTCTTTCTTTCTTTTTTTTCTTTTTT870.10414546847504699No Hit
TAAACAGGGATTAACACCAGGAACAATGTATTTTATGCTTCTTTGTGGTAAG870.10414546847504699No Hit
CTTCCAGGGTACACAAGTGAATGAAGATTGTGTGGGCGACGGGGTCTGCGTG870.10414546847504699No Hit
GTCCACGGGTCTTTCCATTTGTTCAAGTCTGTGCCTTTCTTGGGGTGCTTTT870.10414546847504699No Hit
GCCGGGGTTGTGTTAGTTTAAAACGATAATAAAAAGAAAGAAATTAAGTCTC870.10414546847504699No Hit
TTTAGGGGCATAAATGTACCCAGTAACCAGGTTTTCTAGAACACATTTATTT870.10414546847504699No Hit
GGTGTGGGGCAATATTAGATAACTTTCAGAAACAGCTACTGCACAAAGAAGA870.10414546847504699No Hit
AATGGCGGGTAACCTTTTTCTTCTGGTCTTTGCAAATCCTGCCGTAGAAACT870.10414546847504699No Hit
CACCACGGGGAAAATGTTGGGTCGTAGACTTATTGAGACCTGTTAGGTACCT860.10294839412475908No Hit
GGTCACGGGATATGGAGTGTATTTTCTCCAAAACTTCTCTACGAGGGAATGC860.10294839412475908No Hit
ATGTAAGGGGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGAAAAGAAT860.10294839412475908No Hit
ATTACAGGGCTGAGAGATAGTTGTTAAAATTCCAATTTACATTGTGGATTTT860.10294839412475908No Hit
ATTTAGGGGGGGTTTCATAGCTGTGGAAAAGCCAAAGGGGAGACTCCTGAAG860.10294839412475908No Hit
ATATCCGGGGGCTGACTGTCTAATTTGGAGTAGACATTGAAGTCATCATTAA860.10294839412475908No Hit
AAATTAGGGATAGTAGAAATTAAAACATCAACAAATGGATTAGCAAAATAAT860.10294839412475908No Hit
AATTCCGGGAGTTACAGTGGTGAAAGTGGGCATCCTTGCCTTCTTCCAGATC860.10294839412475908No Hit
ATGCGTGGGACACATATGCAGTAACATGAAAATAAGATGTCAGCACCCATCC860.10294839412475908No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCCTGTCTCTTATAC850.10175131977447119No Hit
TACACAGGGGATCAAACCAGCCACACAGCATTCCTTTAAGCTAAAGCTTAAT850.10175131977447119No Hit
CAAACAGGGGTCTTACTCTTAAACCTGGTAACACTTGATTTGCCTTCTATAA850.10175131977447119No Hit
TTTGACGGGTGAGTAAATGGCAAATACATGAAGCAATGTCCCTAAATTTTAT850.10175131977447119No Hit
CAATATGGGTGCCATGGAAAAATGCAAGTTATAGCATATTTCATTTTTATAA850.10175131977447119No Hit
TAGGGGGATACACACACAGCATACTTTTCAAGTTAATTCAGCACACCTTAAG850.10175131977447119No Hit
GTGGACGGGAGAGACACCTCCCAGTAGGGGCTGACTGATACCTCATACAGCT850.10175131977447119No Hit
GGTTCTGGGCTTTTTGTTTTCTTTCATTTTCATGCAGGGTTGTGTGTTTGTG840.1005542454241833No Hit
AAAAGTGGGAGTGTAAATTGGTTCAATCAAAATCCTTTTTCTGTATCCTTTC840.1005542454241833No Hit
CTATTGGGGGCAACTTAGAGCCAAGACTGGTGGCCACCTCCATTTATTTCCC840.1005542454241833No Hit
TTTAGAGGGCCATCTGTTAACCTCTGAGTTTGCCCACTCAAGGCCACTCATA840.1005542454241833No Hit
TTCTATGGGGGACCGTGGAGGGGTGCAGGCCGGTCGCCTCGGACAGGGCCTT840.1005542454241833No Hit
TTAACGGGGGGATGCAGCAAAAGGAGTACTAAAAGGGAAGTTTATAACTAAA840.1005542454241833No Hit
CTCCGGGTGAACTGATTTAGTCCTAATTCCAAGAAGTGTGATTCCACCTACT840.1005542454241833No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)