FastQCFastQC Report
Sat 31 Dec 2022
SRR5849282.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849282.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84800
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT5550.6544811320754718No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC4720.5566037735849056No Hit
TGTCCAGGGATTTTCATGAGTCAGCAAAAGCCCTACGCTTGATTCCAACAGA1420.16745283018867924No Hit
TTAAGGGGATGCTGGAAATGGAAGCAGGCTTTTCAGTGCTTTGTGGCAGTCT1350.15919811320754718No Hit
GAGTTAGGGAGCTCAAGGAACTAGATAAGCAAGAAAAAAACAAATCCAAAAT1340.1580188679245283No Hit
ATTTCCGGGACATAAAAAGAATTTTGTGTAACTGGCAGTTTGAGCAACAATG1320.15566037735849056No Hit
GCTGCCAGGTCTTTTTAATAAAGATTAATAAATTTTACTTCATACTACATAT1300.15330188679245285No Hit
ATGTAAGGGGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGAAAAGAAT1200.14150943396226415No Hit
GCTGGCGGGGTGGCTTCTAAGTAAGTCCTGCAGTCTTATTTCTCACTTCTGT1180.1391509433962264No Hit
CTATTGGGGGCAACTTAGAGCCAAGACTGGTGGCCACCTCCATTTATTTCCC1170.13797169811320756No Hit
CATTACGGGAACTACACAAAACTGACCAATCTGGGTTGCCAAAGCAGCCAGG1150.1356132075471698No Hit
AAATATGGGAACAAACTGAAGAATTGTCTGAAAGAAAATATGGCATGTCTAA1130.13325471698113206No Hit
GGAGCCGGGGGAGCCAACTCAGGCCATTTCCCCTTGCTGTGGTTTCTTACTG1130.13325471698113206No Hit
TTAGTCGGGAATTTGGTCTTTATTTCGAAGGTGGTAGAAAGCCCTAACTTCT1120.1320754716981132No Hit
CGACCGGGGATACTATACGTGGTCTTTTGTAGCTGGCTTCTTTCATTTAGCA1120.1320754716981132No Hit
ATGCTGGGGGCGTCGCTTTCAGCCTCTGGTGAAGGGCGGCGCGCTTAGGCAG1110.13089622641509435No Hit
CAGCAAGGGGTGCTGAGAGGAGAGTCATGAGTCTGAGAAGGGCACCATTTCA1110.13089622641509435No Hit
ATTTTTGGGATATTTAATACTTTTCTCATATGTAAGTTATGCATGTCTAGAG1110.13089622641509435No Hit
ACTTCGGGATGTTTTGGCATAATAGGGTTTTAGTCATGTAAGGGATATGTCA1100.12971698113207547No Hit
TAAATGGGGGGGGCAAGTGACTTGTATGCCACTGAAGCAATTTCTTGCTTTA1100.12971698113207547No Hit
ATGCGTGGGACACATATGCAGTAACATGAAAATAAGATGTCAGCACCCATCC1090.12853773584905662No Hit
GAATCCGGGACATTGATAAATTTTTAGCATTTTAAGCATATCGGAATTATTG1080.12735849056603774No Hit
CTCGCTGGGTCAGGAGCTCAGTTTCTTTTTCACTTGGGTCTCTGGATTCCTG1080.12735849056603774No Hit
ATGGCGGCGGGATCTACTTGCGGGTCCATCAGTGCCAGCATACCAATGAACC1070.12617924528301885No Hit
GATGGTGGGTTACATGTGCCATTTTTTTAATTCGAGTAACCCATATTTGTTT1060.125No Hit
GCGACTGGGAATTTAAATGAACTAGTTAAACATGGTCTGCGTGCCTTAAGAG1060.125No Hit
CTTACAGGGCACAGAAACCAAGATGGAAGAATTCAAGGACCAATTACCTGCT1050.12382075471698113No Hit
GGCACGGGGGACGCTCTTCCGATCTGGGCATGGGCTCTTTCCCTTCGGTGTG1040.12264150943396226No Hit
AAGAATGGGAAGCATTGTGCATGATGTCTTTCTTTAGAAAGCTTGATTATGT1030.1214622641509434No Hit
CGTTATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1030.1214622641509434No Hit
CAATATGGGTGCCATGGAAAAATGCAAGTTATAGCATATTTCATTTTTATAA1030.1214622641509434No Hit
AAATTAGGGATAGTAGAAATTAAAACATCAACAAATGGATTAGCAAAATAAT1030.1214622641509434No Hit
CAAACAGGGGTCTTACTCTTAAACCTGGTAACACTTGATTTGCCTTCTATAA1020.12028301886792452No Hit
TGTAGGGGAGGGCCGGCGAGCGCAGTGCAGCGCCGTGCGGTGCGGGCGGCCG1020.12028301886792452No Hit
TGCTGTGGGATAACAAAAATCATGTGAAAACCAAAATTAAAAACATAATAGT1010.11910377358490566No Hit
ATTCTCGGGCACTCTAATGACTTTGATGAATGTCCTTAAATATGTGTGCACC1010.11910377358490566No Hit
TTTAGGGGCATAAATGTACCCAGTAACCAGGTTTTCTAGAACACATTTATTT1010.11910377358490566No Hit
CGAAGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1010.11910377358490566No Hit
AGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGAT1010.11910377358490566No Hit
CTTGTGGGGCTTATCAAGCGTAACACGACCGTGCCAACCAAGAAGTCGGAGG1000.1179245283018868No Hit
TGGACCAACGGGAAGTGAATGGAAACTTATTCCATTTTTTTCAAATATTCTC1000.1179245283018868No Hit
TGCCAAGGGGCCATGGCATAAGGAACTCTATCCCAGGAATACAGCTTTGCAT1000.1179245283018868No Hit
CGTAGCGGTATGCAGAAACTGTGATGACTTTGATTTTTGTGAAACGTGTTTC1000.1179245283018868No Hit
TCTTTGGGGGAGGTTATGGATACCCTAAATACTCTGACTTTATCATTATACA990.11674528301886794No Hit
TTACGTGGGTAGAGTCATTTCATTTAACACAGGAACTGAGACTTTTGAAGGA990.11674528301886794No Hit
AATCTAGGGGCTAAACCTCCTGCAAAAGTGGAAGCGAAGCCGAAAAAGGCAG990.11674528301886794No Hit
TCTTGCGGGCATATGGTTCCAAGTTGATTAAGCTTTGTTACCAGCAGACTAT990.11674528301886794No Hit
TTAACGGGGGGATGCAGCAAAAGGAGTACTAAAAGGGAAGTTTATAACTAAA990.11674528301886794No Hit
GACGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA980.11556603773584906No Hit
ATTACAGGGCTGAGAGATAGTTGTTAAAATTCCAATTTACATTGTGGATTTT980.11556603773584906No Hit
GTCCCAGGGAAGGAACTGTATTTTTGCACAGTGATATATCTTCAACATATTA980.11556603773584906No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCCTGTCTCTTATAC970.11438679245283018No Hit
ACAAAAGGGCAGAGTGAAAATGTAATCCACAGAATGGGAGAATATATTTGCA970.11438679245283018No Hit
CAAAGGGAGTCATGAATCAGTTGACAACAAATTTATTGCACAGAATATTTCT970.11438679245283018No Hit
GTCTTCGGGTATAATTTTTAGGTAGATGTTTGGTTGTTGTTTTGATCAAATT960.11320754716981132No Hit
TGACAGCGGGGTCCTTCAGAAAGCTTGATCAGTTTAAAATTGCTTTCTAAAT960.11320754716981132No Hit
CATGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA960.11320754716981132No Hit
CACCTGGGGCAAATATTTATTTTTGTAAGCCTAAAAGTGATTCTTTGAAAGT960.11320754716981132No Hit
AGGACTGGGATTATGACTTTTGTTTTATAGTTCTCTTTTCCAACTCATCTCT960.11320754716981132No Hit
ACTACTGGGGCAGAAGGCAAGAAATAATTAAGATCAGAGCAGAACTGAAGGA960.11320754716981132No Hit
CAGCCGGGACAAAGAAAGCACGAAGTTACTTTAGAATAGAAAATAAAATCTT960.11320754716981132No Hit
TGCATAGGGCAGTAAGGCAGAATTTGTCTTTCCCAGATTCAATCCATATGGA950.11202830188679246No Hit
GAAACGGGGGACCTTTGCTTATGCAAATCAGAAAAACAGAATGGACTAGGAC950.11202830188679246No Hit
ATCCTCGGGACCTGATAAATATGTATCTAGTTCTACAAGGTCACATTTATGT950.11202830188679246No Hit
CAGAACGGGGAACTAACCCTTATAATATAGTTTTACTTATTTTGTTTACTCT950.11202830188679246No Hit
AAGCATGGGATAGTAAGTGGGAATACATACATGGATAAACATTTATTCCAGA950.11202830188679246No Hit
TAATTGGGGCTTGAAGAGAGAAAGCAGAAGGAGCAGAATCGGTTGGAGAAAA950.11202830188679246No Hit
CGTATGGGGATTGATGATGCAAACTACTCCGTTGGCCTGTTGGATGAAGGAA950.11202830188679246No Hit
ATTTAGGGAGTTTAAGGCATTGATAACTTACAAGTAGAATGGGGCTCTCAAA950.11202830188679246No Hit
TATCCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA940.11084905660377359No Hit
CTTCAGGGGAGGATGATGGAGGGGATGAGAGGAGGTTCAATTCTAAGTGTAC940.11084905660377359No Hit
GGATAAGGGGGTTGCAGCATCAAAGAGACAGTGTGGCATACCAATGATAATG940.11084905660377359No Hit
CTCCGGGTGAACTGATTTAGTCCTAATTCCAAGAAGTGTGATTCCACCTACT940.11084905660377359No Hit
ATCACCTGGGAGAAATGAGATAAATGGCAACACGATAATAGTGGGGGACTTC930.10966981132075472No Hit
GTAAGTGGGACTTGCATTAATGGCAACTCTTGCAGTTGCTCTGCTAGAAACT930.10966981132075472No Hit
ATCGTTGGGATTGTATGAAATGCTAGAAAGATTAGAGTAAGAGAAATTTTAC930.10966981132075472No Hit
CTTGGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA930.10966981132075472No Hit
TGTTCAGGGAGGCCATTGATATCGCAGTTCTTCCTATCAGCTTCAGTGACCC930.10966981132075472No Hit
TACCTAGGGCAGCACTCAGGATGTGTTTGTGATTGGAGCCACCAACAGACCA930.10966981132075472No Hit
TTCAGTGGGAGGGATAATTTAGCACATTTTCAGTGAAATGATAAGGCTTTTC930.10966981132075472No Hit
TAAGTAGGGTGAATCAAAATGTGGTAGTTTGGTATTTCAGATAGCTAACTTT930.10966981132075472No Hit
TGTACTGGATAAACTGATTCCTGTATTTATAAGCTGCCATTATGTGATACGA930.10966981132075472No Hit
TCTGTCGGGAGATCCTGTGAAGATAATGACCAGGGCTTTCCCTGTGTATATA930.10966981132075472No Hit
GTCCACGGGTCTTTCCATTTGTTCAAGTCTGTGCCTTTCTTGGGGTGCTTTT920.10849056603773584No Hit
GAATTTGGGAAGGTTGCTGATTTGTGCAAAGACATGAATAAATCTTAAGTAG920.10849056603773584No Hit
GACAGTGGGCAATTTTTACACTGTTATAAAGAAGATGAACTTATACATAATT920.10849056603773584No Hit
CTATTTGGGAGGTCCACAAGATCTTATTCCAATCTGACAATTGACATCAATG920.10849056603773584No Hit
TGTCCAGGGTAAGCAGAATAAACAAAAACCAGAATCTCTTGAGAATAGTAAC910.10731132075471699No Hit
ACACCAGGGGAAATAAATATCCATGAGTTTGTAGGGTTAAAATTAATAAATA910.10731132075471699No Hit
CCGCTAGGGGATCCTTGCCCTACTCAATTCCTTGATATCATCTGCCTGGTAA910.10731132075471699No Hit
TTTCTAGGGCATGCCGGGAGTGATCAGTCGGACATTTTAATTTGAACCACGT910.10731132075471699No Hit
CTTCGTGGGAAAACTTCTGAGTGACCACCACATCTACCTGGAAGGCACCTTG910.10731132075471699No Hit
CTGCAGGGGGAGGATTTCAGTGTTTTGTCTGGGTTGTTTCTCCCTTTACACA900.10613207547169812No Hit
TCGACTGGGCTGACAGAAATTGAGGCAGCTGTTTGCTTATGTATTTGTTTTT900.10613207547169812No Hit
TTTCACGGGAACTCTTTTTTTCCAGTGTTTCAGGAAGTTTCTCTTCCTCCCA900.10613207547169812No Hit
TGCGTAGGGATGGAAAATATTCACAATTTAAGGAAGCTATTTAATTTTTATT900.10613207547169812No Hit
ACCTAAGGGATGAACCATTACTGAAACGTCTTGGTGCTGCCAAGCATTAGGT900.10613207547169812No Hit
ACTTTGGGAGCACACAATTAACCAACTGGAAGAATTCAAGTGAATGTCTTTC890.10495283018867924No Hit
TTGGATGGGATGAGGACTGTGGTTGTTTTAACAAGGCACCTTACAAATGAGT890.10495283018867924No Hit
GTAGTTGGGGTCTAGTGACACAGGAGTGTCCAAAAAGAAAAATAGTAGTGAC890.10495283018867924No Hit
ATACTGGGCAGCCTTAAAAATTTTCTTTCTTTCTTTCTTTTTTTTCTTTTTT880.10377358490566038No Hit
TCTAGTGGGAACAAAAAGGGAATTATTCATTAATGAGAATGATAATACTAAA880.10377358490566038No Hit
AATTCTGGGTTCTAAGTGATATGGTACACATTTCTGCTTCCAAAATGGGATA880.10377358490566038No Hit
GACTTTGGGGGAAACTCTGTTGTAGAAATATTGGCATCCGTTCAAATTCTAT880.10377358490566038No Hit
AACATTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA880.10377358490566038No Hit
GATTCAAGGGGGCACTGTAGGATGTTTAGAAGCATCCCTGGCCCCTACCCAC880.10377358490566038No Hit
TACATGGGGGATCTTAATCCGGGATCTTCAGAGTTAATATACCTTTTCAGAT870.1025943396226415No Hit
TAAACAGGGATTAACACCAGGAACAATGTATTTTATGCTTCTTTGTGGTAAG870.1025943396226415No Hit
GTATATGGGCGATCTAAAACGGGGAAGGACTTCCTGCAGAAAGTCTTTTCTT870.1025943396226415No Hit
GTACACGGGTACTTCTTAGGATCATTAATGCATTGTTCCTAAGTCAGAGTTC870.1025943396226415No Hit
TTAGGTAGGGAACATTCATCTTGTACATCTCCATCAGAGCTCATGAGTGACT870.1025943396226415No Hit
CATCGAGGGGTCTTCCGGTCGCGGGACACCGGGTGTAGAGGGCGGTCGCGGC870.1025943396226415No Hit
CAGCTGGGGGTACATTTCAGTTATTTTTTTTCAAGTTCTTCAGATTGTGACC870.1025943396226415No Hit
AAAGCTGGGATCCTTTGTGTTGCAAACAATCCAGTTATACTCTTTTACTTAT870.1025943396226415No Hit
TGTCATGGGTGCAGAGGACTCTGGGACACTCCAACATTTAAAGGGCACACTC870.1025943396226415No Hit
ATAAACGGGCGATCTTCGCATGGGCATTTACCTCTTATCTCCACCTTGAAGA870.1025943396226415No Hit
CACAAAGGGTCTTACGCCGGGGTCTGAAGAAATTCACTCAAAACCACACGAT870.1025943396226415No Hit
TGTGAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC860.10141509433962265No Hit
GTTGACGGGGCTTTAATGACTATTTATGCGTTGACTGTGAGAATAGGGAGCA860.10141509433962265No Hit
CTACATGGGCATGACTTACTTTATAAATAATGTTTACATGCCATAAGTCCTT860.10141509433962265No Hit
TTAGGCCGGGAGAAGGAAGATGGCGGATCTGGAGGAGCAGTTGTCTGATGAA860.10141509433962265No Hit
CTGCTGTTGGGGCAATTTCACATAGAAAATGTTTTACTAAAAACAGAAATTT860.10141509433962265No Hit
CATAACGGGTGCTAACAGGCTTCAGATGTGTCTAATCACAAGAAACACTTGT860.10141509433962265No Hit
CACGCAGGGTTATCAGGAAATGTTTTCTTAAGCTTTTCCTTTCTCTTACACC860.10141509433962265No Hit
TAACTGGGTGAAATGTCTGTTATAATTAGGTTATTAGTTTCCCAGAGCATGG860.10141509433962265No Hit
CACAGGGATCCTTGAAATTCTACGTTTCTGATTTTAACATCTAAAATTTTAA850.10023584905660378No Hit
CTTCCAGGGTACACAAGTGAATGAAGATTGTGTGGGCGACGGGGTCTGCGTG850.10023584905660378No Hit
TGATTTGGGTCAAGATATTCTCTTTAGACAAAGAGAAGAGAAAACATTTAGT850.10023584905660378No Hit
ATATCCGGGGGCTGACTGTCTAATTTGGAGTAGACATTGAAGTCATCATTAA850.10023584905660378No Hit
TGCGCTGGGTATTGATAATGTGGGACATATGTGTGATTCAATATATACATAT850.10023584905660378No Hit
GAAATTGGGTAATTTAGTTTTTCTTATTTTATCTACAACAATGGCTCTTAAA850.10023584905660378No Hit
TGTAACGGGCAGACACCCGCTGATCAGAGACATGCTTCGACGAATTAAGGAA850.10023584905660378No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)