FastQCFastQC Report
Sat 31 Dec 2022
SRR5849277.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849277.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143732
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC10240.7124370355940223No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT10230.7117412963014499No Hit
GCCAAAGGGCGCTGCATCAGCACCACGTCCAGCCAGCGGCCGAACTTCCAGC1780.12384159407786714No Hit
CAGTGTGGGTGGGAAACAGGTTTCTTTTGTAAGTTTGCTAGATTCTAAAAAC1600.11131828681156597No Hit
GCCGTAGGGCAACCTCAATGGTATTCCTACAGGAAATGGATAACCATTTTAA1580.1099268082264214No Hit
ACGTCCGGGGGGCCCTCGTGGGGCCACCTTCCTGTCAGTGCCCGAGGGACGG1560.10853532964127682No Hit
AATGCTGGGATTACAACACCTGTGAGTACCCCAACGAGGACGAGATGCCCAA1530.10644811176355995No Hit
TATTGTGGGCCATCTCAAAAGAAAATCAAATGAAATAGAAAGCAGAAAAACA1510.10505663317841539No Hit
CAATTGGGGAACTGTTATAGGCTAGCAATGTTGATGGATGTTAAAAATTAGT1490.1036651545932708No Hit
CAAAATGGGATGACCACTGAGAAAACTAGAAAAAAATGAACTTTTAACTCAT1470.10227367600812623No Hit
CAAAACAAACAAACAAACAAAAAAACCTATTTGCAAGTATTTAAAGGAAAAC1470.10227367600812623No Hit
CACAAAGGGTCAATTCTGAAGGAAGACTTCAGTGAGGCCTAGTACTTTCTCA1450.10088219742298166No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)