FastQCFastQC Report
Sat 31 Dec 2022
SRR5849263.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849263.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95979
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT8280.8626887131560028No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC7540.7855885141541379No Hit
TGAAACGGGGCTTTAGAGGCATATCATAAATTTCTATATGTATTTTTTTTTT1390.14482334677377343No Hit
AGAAATGGGTCCCTGACAACCCTGGTAATATGGATGAAAAAGAAGTTTATCT1340.139613873868242No Hit
GGCACAGGGCACTAAAAATGCCTACTCTTCCTGTTGATGTTCCTTTTCTGTT1330.13857197928713572No Hit
GTAGTGGGGATTCCTACTTTACAAGGACTTTTTGAGGTTAAATTAGATAGTT1280.13336250638160432No Hit
ACGCTTGGGGTTTTAAGACAGGACTTTTTAAATTTACAATATTTTTGTCCTC1220.1271111388949666No Hit
ACTTTAGGGTTAATAGTAAATGAGTTTTTGTGTTTCTGCAGATGTGACTCTG1220.1271111388949666No Hit
CTATTCGGGTAAGTTTCCATTATCTGAAATAGTAAAGAGAGTTTAGGAATTA1210.12606924431386032No Hit
GCTATAGGGAGAACTGCACTAGGATTAATTTGTTTACAAGAAGAAATTTAAA1210.12606924431386032No Hit
CAACAGGGGAGAAAGAAAAATATGAAAAGAGAAAAATATTTTAATTTTAAAA1200.12502734973275403No Hit
GGTACTGGGCCAGCTAGTTCACTCCTTTCGCCGTGCGTGGTGGAGGCTGGCC1190.12398545515164776No Hit
TCGTCGGGGGGAGGTAAAAAGAGATTACATAGTTTGTCCTTAGTCTCAACTG1190.12398545515164776No Hit
TAACTTGGGATACACTTAATTCATTATTTTTACCAACTGAGAATATTCTATT1190.12398545515164776No Hit
TATGCAGGGTAATAAATTAACCTTAGCTTACTGTCACATTTTACATTTAAAC1180.12294356057054147No Hit
AAGCGTGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA1180.12294356057054147No Hit
CATGCGGGATGCTTTAGAATGAATACGAACAAGTTTTCTGAAAGTAAGTGAA1160.1208597714083289No Hit
CAAAGCGGGTTGAGATAATCATGTGGTTTCTGTCATTGGTTCTGTCGATGGA1160.1208597714083289No Hit
CGTAACGGGATGATTATTTTCAATTATTATTAAGGTTTTTTACAGTCCGGCT1150.11981787682722263No Hit
TTTCGGGTGGAATGATTAATGGAGAAACCATGAAAATATTGGTAATTTTTTT1150.11981787682722263No Hit
AGCGATGGAAGAATAAAGAATAACAATAAGAAAATTTAATACATGTAAAAAC1140.11877598224611635No Hit
GTCACTGGGATTCAGTGAATTACTCAAAATACATAATGAAAGGAGAGTTGCC1140.11877598224611635No Hit
GTATTATCCCCATTTTACAGAGAGGAAGCCGATGGGACTTTCCTAAGGTCAC1140.11877598224611635No Hit
GCACTGGGGAAAAAAAGGAAGTGTTTGGACGACAGCGAGCGGGAGAAGCGGG1140.11877598224611635No Hit
ATGGCTGGGAGCTGAGAGTGAAGCAAAAGTAAAAACCAAAGTTCGCTTTGAA1120.11669219308390379No Hit
GCAATCGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT1120.11669219308390379No Hit
GCCTCGGGGGAACTATAAAACTAAAAAAGTACAAGGAAAAAAATTAAAATCA1110.11565029850279748No Hit
TCTTAAGGGAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGCCCGGGCGT1110.11565029850279748No Hit
TGGCATGGGAACCTAGACCAGTCCTGTTTGCCTCATGATATCAGGTGGGAGC1100.1146084039216912No Hit
AAAAGCTGGGGAGTGGCTAAATCTTACCACTTTCTTTCTACTTTTTTTGGAA1100.1146084039216912No Hit
AAGCTTGGGATGAGAATTTCTAATAATGAGAATTTTACTTTGTCCTCATAAA1100.1146084039216912No Hit
AACGCCGGGATGCGTGCAAAGGCACGATTGATTTATCATGCTGGCTTTTATG1100.1146084039216912No Hit
GGATGCGGGGGCCCACGTGGCCTCCGCGGGCCCCGCCAGAGCCTGCGCCCGG1100.1146084039216912No Hit
GGTCTGGGGAAGATATACCATGCAAACAGAAACCTTAAAAGAGCTAGAGTGG1090.11356650934058492No Hit
AGTTTCGGGCGAAGTGAGACATTTAAAAAAAATGATAAAATTGTCAACTCAT1090.11356650934058492No Hit
CTCGCCGGGATTTTAAGGAACTGGCTCATGCAATTGTGGAGCCTTGGTACGT1090.11356650934058492No Hit
GTAAATGGGCATTCCTGCATATTCTAATGTATATGATGCAAATGTGTGTCTC1090.11356650934058492No Hit
TGCACTGGGAAGTCATTTTAAAGAAAGTTAGTTCATAGTTGTTGCCTTTTAA1080.11252461475947863No Hit
GATGTAGGGATACTCATTTAATTAAGATTTAATCATTTGCTAATGGAAATCT1080.11252461475947863No Hit
GATTAAGGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGGTTGAGA1070.11148272017837235No Hit
AAAACAGGGGCGGCAGGAGGGAAGATGGCGGACGAGGAGAAGCTGCCGCCCG1070.11148272017837235No Hit
TTACGGGGATTTAATGAAATTATGCATTACCACATTCAAACACAAATCCATT1070.11148272017837235No Hit
ACAGTAGGGGGACCATAAGGTACATCATTTATCTTGAACATTCCGACGTGTA1060.11044082559726606No Hit
TAGTCTGGGAGCATGTGTTAAAAACGTGTGAATTATTTATCCTGTAGTATCT1060.11044082559726606No Hit
TGTTGAGGGATACCAATAGCTCAGGATTTGACTATTTTCATTAAGCCAACAA1060.11044082559726606No Hit
ATGCCTGGGGAGATGAAGCAACATTATCTCTCTCTAATCTGCCAACAATATT1050.10939893101615979No Hit
GAACTGGGGGCCACTCCTCCTTTGGACTGTTTCTTTTGTGAGAGACTAAACT1050.10939893101615979No Hit
GTTTGCGGGGAAAAGATCCAGGAAAATATTGCATTCATAAAACAAGTGGATG1050.10939893101615979No Hit
TCCAACGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT1040.10835703643505351No Hit
GCATGCGGATTTATCTAATTCTCCCTACTGAAATTCAGTCTTTTTGAGAGCA1040.10835703643505351No Hit
GGGCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1040.10835703643505351No Hit
ACGCATGGGAACATTAGAGTTGGAAGTAAACCATGCAGGGAGAAAAAGACCA1040.10835703643505351No Hit
ATGTGTGGGCAGTTTAAAAAAGCAGTGACAATGTGTCCATTTCCACCAAAGT1040.10835703643505351No Hit
ATCCATGGGATGTTTTTTGTGTACTATCTGGGAAATTACTGCCTTACCCCAA1030.10731514185394722No Hit
ACATCGGGGGATGTCTGCCTATATTTTTATCTCAAGGTTAGTTATTGTCAAA1030.10731514185394722No Hit
ATTAGAGGGATAAAGAGTGGTTTTTAAAAGACCTTACTATGGAATTTTCACA1030.10731514185394722No Hit
TAGTGGGGGCGTTTATCATAAGCTATGTCATTAGTCTCAGCTTCCTAGTGGG1020.10627324727284095No Hit
GTCTCAGGGACTCGGATGGTACACAGATACTGTCTTGATGGGAGTTTTCGGA1020.10627324727284095No Hit
GACTGCGGGGGCGGGGACCGGAAGGATGGCGAGGAAGTCGGAAAGGGTTTCC1020.10627324727284095No Hit
AAATACGGGATGGAGGCTCGGGCAGCTATTCGGTATGTTCAAACTCAGACAC1020.10627324727284095No Hit
TAATGCAGGGGGTAGCAAATACATTTTGGCTTAAACAAATTAGGGATTTTTT1020.10627324727284095No Hit
AGCCGCAGGGGTTTTCAAAAGGTGCTAACAAAATATCTAACAGTAGCAATAC1020.10627324727284095No Hit
TAAACGGGGGTCCGAGCCTCAGGGCATGTAACCTTACAGCTCCGTGGTGGAG1020.10627324727284095No Hit
GGTAACGGGGAATAACTTGACCCTAACCTGTAGAGAATAAGAGCATTCAAAT1010.10523135269173466No Hit
TTGCAAGGGACCAAAGAAAGTACTTGAGCAGAAGAGCCAAAATTCAAACCAT1010.10523135269173466No Hit
GTAGTAGGGCTGTACTCTGAGGAGGGGAAGGGCATCTTCGACAGCAGGAAGA1010.10523135269173466No Hit
CTTACTGAGGAAATGAACAAAGGATGGGAACGGACATTTCTCAAAAGAAGAG1010.10523135269173466No Hit
GACTATGGGATAGATCCACACACAGCTGCTAGTACCACTGCTGAGATCATCT1000.10418945811062835No Hit
CAAGTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1000.10418945811062835No Hit
ATCATGGGGTATTGTGATTGCTAAAATAATTCTTAAGCTACTTATATACATA1000.10418945811062835No Hit
TTCCCGGGAGTCAGTTGAAAGAAAGACAAGAGAGTCACATCTTAGCTTTTGC1000.10418945811062835No Hit
CAATACGGGAATTTCTGATATTAACAACCGTACATACTGCTGAATTTAACTC1000.10418945811062835No Hit
GCTTATGGGCTGCCCAGCCACCTTTTCAGAAACCCAAATAATTCCTATAATA990.10314756352952208No Hit
TACAAGGGGGACTGAGGCGCGCTGTTCTGGGACCGGAGGAGTGGGGTTTCTA990.10314756352952208No Hit
TAGGGGCTTGAATTTGGCTGAGGGACAGTTATTGTACCACTTACCTGGAGAT990.10314756352952208No Hit
CATTGGTAACTTCAAGTAAGGTATCTATAGAATATACCCAAAAGGAAATGGG990.10314756352952208No Hit
TAAACCGGGATAAAATTGTTGAAAATAAAATCCATTCATGCAACTAACTTAG990.10314756352952208No Hit
ACGACAGGGTGTGGCCAGTTTTCGTAATGAAACAGTCTTTAGTAATTTAATC980.10210566894841579No Hit
CTACCTGGGTCACAGCGGGTTTCTGGACCAAAGTTAATACTTTCCTTTCTAG980.10210566894841579No Hit
TGAGATGGGAGCCCTTTAAGATGGCCAGGAGCTAAGCTTGAGTCTCCTTTAC980.10210566894841579No Hit
ACTGGGCTACCGCATTCTTATGCAACTCTGTGGACAATATGATCAGCCAGTG980.10210566894841579No Hit
AAGTCTGGGTAAGGGGCCTCTTGCAGTTCACAGGTAAATGTATCAATCAATA980.10210566894841579No Hit
AGGCGCGGGATCCAATTTGCCAGTCTGTGTCTTTTAATTGGAGCATTTAGCC980.10210566894841579No Hit
TACATGGGGCTCTTCCTGTCTGTACCAGGGCGGCGCGTGGTCTACGCCGAGT980.10210566894841579No Hit
ATCCGCGGGGGATCATGGATCCTCATTATTCCCATAAGGTATGTATAGAAAG980.10210566894841579No Hit
GACCTCGGGATCAACATGTTTTTGATATTTGATAATGAAAAAAATCTTTGCT970.10106377436730951No Hit
ATGCATGGGCAACTCAGAATGAGGGAGAATTCTTTCTTTTTAAAAAAATACA970.10106377436730951No Hit
TGAACAGGGATATGGGTTATATTATTGTGTCTTTGTCAAACAACAAAAACTT970.10106377436730951No Hit
TTTACTGGGGAAGCCAGTCATACATTAATTCTATTGAAATAAATGCTTTACT970.10106377436730951No Hit
GGATTCGGGAATCCAATTGCCTATTTTTGTCTTTTCATGTATGGTGAATACA970.10106377436730951No Hit
ATTACTGGGAGATTAAACAAAAAATACTATAAAACCCCTGACCACATTTAAA970.10106377436730951No Hit
CTACTGGGGGCACAGCCGCGCGGGCGGCGGCCAGGAGTCGGAGTCGGAGTCG970.10106377436730951No Hit
ATCAACGGGCCCACTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCT970.10106377436730951No Hit
CTTATTGGGGCTCTTTGTCAGCTGGAGTTGCGCGGGCTGACGCGCCACTATG970.10106377436730951No Hit
ATAACAGGGGGCAGGCAAGAGTGCTGGAGGGCGGCAGCGGCGACCGGAGCGG960.10002187978620322No Hit
TGCTTGGGGGATGTCTTTTGATAAAAGAAATTATTTTACTGTAATAGGGTTT960.10002187978620322No Hit
TCTGAAGGGAACATAGTTGAAAAAAGAAGTTTGGGCTAACATTTAGGAGAAG960.10002187978620322No Hit
ATTACAGGGGCATTTATAATTGATTTTATATTGGTTTTACATCCTGTAGCTT960.10002187978620322No Hit
TCTTAGGGGGGCTAGTTGGAGAAGTGTCTGGAATTCTTCACTGGGGCGGGGG960.10002187978620322No Hit
GCTGCAGGGCCACCTGGGCGAGATCCGCGAGCTCAAGCAGCTCAACCGGCGT960.10002187978620322No Hit
CTACAGTAGGGCATCATCACTGGTCATTAGAGAAATGCAAATCAAAACCACA960.10002187978620322No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)