FastQCFastQC Report
Sat 31 Dec 2022
SRR5849261.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849261.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96872
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT8170.8433809563134859No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC7520.7762821042200017No Hit
GGCACAGGGCACTAAAAATGCCTACTCTTCCTGTTGATGTTCCTTTTCTGTT1340.13832686431579816No Hit
CATGCGGGATGCTTTAGAATGAATACGAACAAGTTTTCTGAAAGTAAGTGAA1320.1362622842513833No Hit
GTCACTGGGATTCAGTGAATTACTCAAAATACATAATGAAAGGAGAGTTGCC1320.1362622842513833No Hit
CTTACTGAGGAAATGAACAAAGGATGGGAACGGACATTTCTCAAAAGAAGAG1310.1352299942191758No Hit
GGTCTGGGGAAGATATACCATGCAAACAGAAACCTTAAAAGAGCTAGAGTGG1290.1331654141547609No Hit
AAGCTTGGGATGAGAATTTCTAATAATGAGAATTTTACTTTGTCCTCATAAA1270.131100834090346No Hit
AAGCGTGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA1250.12903625402593114No Hit
GCATGCGGATTTATCTAATTCTCCCTACTGAAATTCAGTCTTTTTGAGAGCA1230.12697167396151623No Hit
GAACTGGGGGCCACTCCTCCTTTGGACTGTTTCTTTTGTGAGAGACTAAACT1230.12697167396151623No Hit
TGAAACGGGGCTTTAGAGGCATATCATAAATTTCTATATGTATTTTTTTTTT1230.12697167396151623No Hit
TAAACCGGGATAAAATTGTTGAAAATAAAATCCATTCATGCAACTAACTTAG1230.12697167396151623No Hit
TAATGCAGGGGGTAGCAAATACATTTTGGCTTAAACAAATTAGGGATTTTTT1230.12697167396151623No Hit
AGAAATGGGTCCCTGACAACCCTGGTAATATGGATGAAAAAGAAGTTTATCT1220.12593938392930878No Hit
GTAGTGGGGATTCCTACTTTACAAGGACTTTTTGAGGTTAAATTAGATAGTT1220.12593938392930878No Hit
GCACTGGGGAAAAAAAGGAAGTGTTTGGACGACAGCGAGCGGGAGAAGCGGG1220.12593938392930878No Hit
TAGTGGGGGCGTTTATCATAAGCTATGTCATTAGTCTCAGCTTCCTAGTGGG1210.12490709389710132No Hit
TACCTGGGGATTAAAAAATGGTCAGTGTGCAAAGAAAAGAACTGCTTGCATT1210.12490709389710132No Hit
TTAACAGGGGTGCATTTGTTTGTGGCAAAAGCCTACAATTGACAAACTTGTA1210.12490709389710132No Hit
CTATTCGGGTAAGTTTCCATTATCTGAAATAGTAAAGAGAGTTTAGGAATTA1180.12181022380047898No Hit
CTAAAACCGGGTAATAGATTTCAAGAAAAAACTGTAAGAGACAAAAAGGTCA1160.1197456437360641No Hit
CATTGGTAACTTCAAGTAAGGTATCTATAGAATATACCCAAAAGGAAATGGG1160.1197456437360641No Hit
ACTTTAGGGTTAATAGTAAATGAGTTTTTGTGTTTCTGCAGATGTGACTCTG1160.1197456437360641No Hit
ACGCTTGGGGTTTTAAGACAGGACTTTTTAAATTTACAATATTTTTGTCCTC1150.11871335370385663No Hit
ATTACAGGGGCATTTATAATTGATTTTATATTGGTTTTACATCCTGTAGCTT1140.1176810636716492No Hit
TTTACTGGGGAAGCCAGTCATACATTAATTCTATTGAAATAAATGCTTTACT1140.1176810636716492No Hit
TCCAGAGGGAACTAAGAAGGGGAGACGTACATAACTTGTAACTTGCAAAGAT1130.11664877363944173No Hit
AAATACGGGATGGAGGCTCGGGCAGCTATTCGGTATGTTCAAACTCAGACAC1130.11664877363944173No Hit
CTCGCCGGGATTTTAAGGAACTGGCTCATGCAATTGTGGAGCCTTGGTACGT1130.11664877363944173No Hit
TCGTCGGGGGGAGGTAAAAAGAGATTACATAGTTTGTCCTTAGTCTCAACTG1120.1156164836072343No Hit
AGGCGCGGGATCCAATTTGCCAGTCTGTGTCTTTTAATTGGAGCATTTAGCC1100.1135519035428194No Hit
CCGGGATATTCTTTAAGTGTTTAATTAATCTCTGGATATGGTAAGATTCCTT1100.1135519035428194No Hit
TTACGGGGATTTAATGAAATTATGCATTACCACATTCAAACACAAATCCATT1100.1135519035428194No Hit
TACCTAGGGCATGCTGCAGCCTGGAACTCTGATCTCCAGTGTGACTGCAAAG1090.11251961351061195No Hit
AGTATAGGGTACTTTTTAATCCTATGAGGTAAGTAATGTTACCATTCTTTTT1090.11251961351061195No Hit
GCCTCGGGGGAACTATAAAACTAAAAAAGTACAAGGAAAAAAATTAAAATCA1080.1114873234784045No Hit
ATCCATGGGATGTTTTTTGTGTACTATCTGGGAAATTACTGCCTTACCCCAA1080.1114873234784045No Hit
ACAGTAGGGGGACCATAAGGTACATCATTTATCTTGAACATTCCGACGTGTA1080.1114873234784045No Hit
AGTTGGGGGGGTAATCCATGTTCTATGTTGTTAATGTGTGTATGTAATTTTT1070.11045503344619705No Hit
TAATATGGGTATGCATCTTGGAGATAGATTGTCCCAGTCTGCCTCATTTTTA1070.11045503344619705No Hit
CACACAGGGTGTGGTAACAGACGTCAGCTGCTTCTTTATTCGGCAATGTGGG1070.11045503344619705No Hit
ATGAGCGGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTAC1070.11045503344619705No Hit
TACATGGGGCTCTTCCTGTCTGTACCAGGGCGGCGCGTGGTCTACGCCGAGT1070.11045503344619705No Hit
TCTTCGGGGAATGAAATTGAAATGAGATGAGATGAAATGAGATAAAATGATG1060.10942274341398961No Hit
GGTACTGGGCCAGCTAGTTCACTCCTTTCGCCGTGCGTGGTGGAGGCTGGCC1060.10942274341398961No Hit
CGACACGGAAGTGAATTGAGCATTAATGTTTCTTTTGTATAAATTCCTGTCC1060.10942274341398961No Hit
ATCAACGGGCCCACTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCT1060.10942274341398961No Hit
CGCTCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1050.10839045338178215No Hit
TCTGAAGGGAACATAGTTGAAAAAAGAAGTTTGGGCTAACATTTAGGAGAAG1050.10839045338178215No Hit
TCGGCCGGGACTGTAGGTGGTTGTAACACAATGGCCAAGTATTTGTATATCT1050.10839045338178215No Hit
TTTCGGGTGGAATGATTAATGGAGAAACCATGAAAATATTGGTAATTTTTTT1050.10839045338178215No Hit
AAGTTAGGGAAATCAAAACCACAATGAGATACCATCTCACACCAGTTAGAAT1050.10839045338178215No Hit
GACTATGGGATAGATCCACACACAGCTGCTAGTACCACTGCTGAGATCATCT1040.10735816334957471No Hit
ATGTGTGGGCAGTTTAAAAAAGCAGTGACAATGTGTCCATTTCCACCAAAGT1040.10735816334957471No Hit
TAACTTGGGATACACTTAATTCATTATTTTTACCAACTGAGAATATTCTATT1040.10735816334957471No Hit
CTTATTGGGGCTCTTTGTCAGCTGGAGTTGCGCGGGCTGACGCGCCACTATG1040.10735816334957471No Hit
GGTAACGGGGAATAACTTGACCCTAACCTGTAGAGAATAAGAGCATTCAAAT1030.10632587331736724No Hit
AGTTTCGGGCGAAGTGAGACATTTAAAAAAAATGATAAAATTGTCAACTCAT1030.10632587331736724No Hit
ACTGGGCTACCGCATTCTTATGCAACTCTGTGGACAATATGATCAGCCAGTG1030.10632587331736724No Hit
CTACAGTAGGGCATCATCACTGGTCATTAGAGAAATGCAAATCAAAACCACA1030.10632587331736724No Hit
ATCCGCGGGGGATCATGGATCCTCATTATTCCCATAAGGTATGTATAGAAAG1030.10632587331736724No Hit
ACGACAGGGTGTGGCCAGTTTTCGTAATGAAACAGTCTTTAGTAATTTAATC1020.10529358328515981No Hit
TTACTTGGGAGGAAGAACTCATGCAACTCAATAGCAAGACAACATATAACCT1020.10529358328515981No Hit
GGGCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1020.10529358328515981No Hit
TCCAACGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT1010.10426129325295234No Hit
GATTAAGGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGGTTGAGA1010.10426129325295234No Hit
TCTTGGGTTGAGCTGTGGGGAGGTTCCAGCAGCAGCTACAGTGACGACTAAG1010.10426129325295234No Hit
GCAGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1010.10426129325295234No Hit
ATGGCTGGGAGCTGAGAGTGAAGCAAAAGTAAAAACCAAAGTTCGCTTTGAA1010.10426129325295234No Hit
ATGCAGTGAGGATTGAATCATATGTCTATCTTATGTCAACATCACACTCTCT1000.10322900322074491No Hit
CCAGACGGGACTGAAAAAAATGAATTTCAAGATTCTCAACTTTTTTAATTTC1000.10322900322074491No Hit
GGGATGGCTGAATCCTTGTGTGATATTTTTTTCTTCGCTTGTTTATTTATTC1000.10322900322074491No Hit
GCTTCAGGATGATTTTTGAAGCAGTGTAAGCATTGCCTTCCTTTGTGCTCCT990.10219671318853746No Hit
GTAAATGGGCATTCCTGCATATTCTAATGTATATGATGCAAATGTGTGTCTC990.10219671318853746No Hit
GGATGCGGGGGCCCACGTGGCCTCCGCGGGCCCCGCCAGAGCCTGCGCCCGG980.10116442315633001No Hit
TAAACGGGGGTCCGAGCCTCAGGGCATGTAACCTTACAGCTCCGTGGTGGAG980.10116442315633001No Hit
TATGCAGGGTAATAAATTAACCTTAGCTTACTGTCACATTTTACATTTAAAC970.10013213312412256No Hit
AATAGGGGGAGGATCGGATTAGAGCGAAGTTTTATTCAGTGTATGGGTCTGG970.10013213312412256No Hit
GCACAGGGGGACTGTGTAACTGAAACACCAGATTAGAGCAGAGCCAGGAACA970.10013213312412256No Hit
ACGTTGGGGATAACATAACATGAATTTCTGTTATGCATAATACAAATTGACA970.10013213312412256No Hit
GTTTGCGGGGAAAAGATCCAGGAAAATATTGCATTCATAAAACAAGTGGATG970.10013213312412256No Hit
AAAACAGGGGCGGCAGGAGGGAAGATGGCGGACGAGGAGAAGCTGCCGCCCG970.10013213312412256No Hit
CCAGTTGGGAGAGTGTGATTATTGTATTAGACAACAACAACAGCAATGACAG970.10013213312412256No Hit
GCAATCGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT970.10013213312412256No Hit
CCTTTTGGGATATAAGAAGACACTGTATCCAACAAGACTGGCTGTACATTGA970.10013213312412256No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)