FastQCFastQC Report
Sat 31 Dec 2022
SRR5849254.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849254.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84865
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC5500.6480881399870383No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT5430.6398397454781123No Hit
AACGAGAGGGGGAGAATGCAGATAATTCACTCATGACTCATTGTTATGGAAG1220.1437577328698521No Hit
CAAATAGGGGCCAAAAAGTAATGAAAAATTAAAAGGCAAGGAAATTGGGAAA1070.12608260177929653No Hit
GTCAGGGAGTCAAAGATAGAGCTAAGGAAAGGCAAAAATGAAACTAAATGAA1070.12608260177929653No Hit
CGCCATGGGACCCAAAACACTTGCTCCAATTTTGCCCCTTATAATTGCCAAA960.11312083897955577No Hit
TCCGAAGGGTCCCTGGGAAAAGGCAGTCTTTTCAACAGATGGGAAAAGTGGA960.11312083897955577No Hit
GGCGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA950.11194249690685207No Hit
ACATTAGGGCTCGACGCCTGCCACAACAGTTTTGCCAGTCACCAAACTCAGA950.11194249690685207No Hit
TGTGCCGGGCTGGGAGTACAGGCATGTGCCACCATGCCCAGCTAATTTTTGT940.11076415483414835No Hit
TACTGTGGGAGACCAATGATGAGTATTCTGGGGTGTCTGAATCAATGATTTT940.11076415483414835No Hit
TTCTCGGGGATTCAGCCTCAGTAGTAGCGAACTGCACTGCTGTTTGGTTTGG940.11076415483414835No Hit
TGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG930.10958581276144465No Hit
AAAATTGGGATGGCCAACTAAAATTGCTTGAAGTAGAAACAGAAAGAAAATC930.10958581276144465No Hit
ATCAAAGGGGTTTGTGTGTATTATGAGCAGTAGTCAACAGGAGCAAATGAGA930.10958581276144465No Hit
GATTTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA930.10958581276144465No Hit
ATCTTTGGGAAGATATACAGATGGCAATTCAGCATATGAAAAGATGTCCCAC920.10840747068874095No Hit
ATACGCGGGGTAGTTACATGTGAAACTCTAAATGCATGGCAACTGTTAATGT920.10840747068874095No Hit
CCTGAGGAGATGCCTATGAAGTTACATCCTTTTTACAATTAATAAGACAGTT910.10722912861603723No Hit
CAACAGGGGAACCTCTCCTAATTCCATCTACTGGCAAAGCTTGATTTGATGA910.10722912861603723No Hit
TCTCATGGGCCAAGTGTTGCGGCGACGCACCTCACGTCGAGAATCGGGAGGA910.10722912861603723No Hit
TGTACAGGGTAACATAAAATGTAAAGCTTAGCACCCATCATTAATTTATGTC900.10605078654333353No Hit
AAGTCTGGGGAAACAATCAACTAAAAACACAACAAAAATGTGTGCAAACCAT900.10605078654333353No Hit
AAAGCAGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA900.10605078654333353No Hit
AAAAATGGGAGCCGCCCTCGGCCTTGCGCCGCCGCCACTACCACCACGCCTT900.10605078654333353No Hit
ACCTGGGGGAGTATCTAGAATTGAAGATGGCTGAGTCTTCAGATGGTTCAAG900.10605078654333353No Hit
ACGGCTGGGGAAGAGTCAGTATTTCAATCTGAGTCTGAAGGCAAGAAACAAG890.10487244447062982No Hit
TGTTGTGGGCTTCATTTTCATCATATAAAGGCTGATAGGTTTCAAGTTCAGA890.10487244447062982No Hit
AATCATGGGAGGAAGACAAAGTCGACTCAACTCTCATTGGCTCATCCTCTCA890.10487244447062982No Hit
CTTAGCGGGGCAGAGCAGAAGCAGCTGCTGGAGGTGGAACTCTACGCAGACT890.10487244447062982No Hit
GCTTTTGGGCACTAACAAAACAGAATGACAATAAAGAAAACATACCTGGGGA870.1025157603252224No Hit
ATTATAGGGCGATCTTCTCACGGGATTGCTGAAGTATTAGAAATTTCCAGGT870.1025157603252224No Hit
TCTAGAGGGCACTCGACCCGCTTAATTTCGGATCCTGTGCTGCTGGTGGGGA870.1025157603252224No Hit
TAACTGGGATGCACCCAGAATCAAAGTTAAGGTTTTCTGACCAACTAACACC860.10133741825251871No Hit
TGATTTGGGTCTGGTCCCTGATCTCGAATATGTAAAGAGCACCTACAAATCA860.10133741825251871No Hit
TATTAAGGGATTCATAGAAAACTGGTAAATGTGTTTCCCTGAGTACTTTGAG860.10133741825251871No Hit
ATTGAAGGGCGATCTACTTTGGGGCCTGAACTTGGCTCAGCTGCCGGGCTGC860.10133741825251871No Hit
TTTATAGGGATTAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCG860.10133741825251871No Hit
ACATCTGGGAGACTCACTTGGCACAGGGTTGCCTAGTTTTGCAATTTGTAAA850.10015907617981501No Hit
AGCCATGGGGATCTCGAAACGGGGGAAATAAGCAACGACAAGCCTGTTCCCA850.10015907617981501No Hit
GAAACAGGGGATAAAAACATATTTTTAGAAGAAAAAAGATAAATTTAAACCT850.10015907617981501No Hit
CATACAGGGGTAAATTTGTCAACTATTTCTGCTGTAAATATACTTAATAAGA850.10015907617981501No Hit
TGTTACGGGTTCCGATCTTAGCTAGGGCTTAGAGATAATGGGGATTGGAAGA850.10015907617981501No Hit
CTCAAGGGTTCCTACTTAACTTCAGTCTCATCTTTGATTGTGTGGCATCCAG850.10015907617981501No Hit
GCTCTTGGGGAGAGGCGGAAGCGGAGTCGGCCTGAGAGGTCTCTCGTCGCTG850.10015907617981501No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTT850.10015907617981501No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)