FastQCFastQC Report
Sat 31 Dec 2022
SRR5849253.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849253.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84727
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC5620.6633068561379489No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT5130.6054740519551028No Hit
CAAATAGGGGCCAAAAAGTAATGAAAAATTAAAAGGCAAGGAAATTGGGAAA1150.1357300506332102No Hit
AACGAGAGGGGGAGAATGCAGATAATTCACTCATGACTCATTGTTATGGAAG1120.1321892667036482No Hit
AGCCATGGGGATCTCGAAACGGGGGAAATAAGCAACGACAAGCCTGTTCCCA1100.12982874408394018No Hit
CGTGGCGGGGGGCGGCGAGCAGGCCCGGGAGCCGGGAGGCTGCGGGCGGCGG1010.11920639229525418No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCCTGTCTCTTATAC1000.11802613098540017No Hit
TGTTACGGGTTCCGATCTTAGCTAGGGCTTAGAGATAATGGGGATTGGAAGA990.11684586967554618No Hit
TTGGTCAGGGATGTGGGTTCTTTGGTCTTTCTTCTGGTCACTCATTAGTCCC990.11684586967554618No Hit
CTTAGCGGGGCAGAGCAGAAGCAGCTGCTGGAGGTGGAACTCTACGCAGACT980.11566560836569217No Hit
GCTCTTGGGGAGAGGCGGAAGCGGAGTCGGCCTGAGAGGTCTCTCGTCGCTG960.11330508574598416No Hit
GGCGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA950.11212482443613016No Hit
AAACATGGGAATCATTTTATCGTGAAAAATCTCAAGAGGTCTTAAAGTTGCT950.11212482443613016No Hit
GTTTCTGGGGATTTGCATATTATGAACCAGCCTTGCATACCAGGGATGAAGC950.11212482443613016No Hit
GTTCGCGAGAGAGAAAGAGGAGCGGAAGAAACAGCAGGAGAAGGAAGACAAG930.10976430181642216No Hit
GTAGTTGGGACGGTTCTTCCTTGCCCTTGCTAATTACAGTCTCTGGTGCCCA930.10976430181642216No Hit
TGATTTGGGTCTGGTCCCTGATCTCGAATATGTAAAGAGCACCTACAAATCA930.10976430181642216No Hit
GTCAGGGAGTCAAAGATAGAGCTAAGGAAAGGCAAAAATGAAACTAAATGAA930.10976430181642216No Hit
ATCGCATAGGGAGAATACAAAATGACCTTGTTACAGTGTGAGGACATCTTGT930.10976430181642216No Hit
ATACGCGGGGTAGTTACATGTGAAACTCTAAATGCATGGCAACTGTTAATGT920.10858404050656816No Hit
TTACTGGGGACTTTTCTAATCCTGCCAAAGAGTTTTTCATAAATTGCTTTGA920.10858404050656816No Hit
TTTGCTGGGAACCTCATCGTATAGAATTTCTGCATTCTAAAGGAAACATAAG910.10740377919671415No Hit
AATCATGGGAGGAAGACAAAGTCGACTCAACTCTCATTGGCTCATCCTCTCA910.10740377919671415No Hit
GTATTAGGGATTTGCTTTGTAACTGGAATTGCAGCCCTCACGTTAAGCTAAT910.10740377919671415No Hit
AACATGGGGGATGGAGGGCTGATCTTGTGTCAAGTTAACAGGAAGACTGCCC900.10622351788686014No Hit
CAACAGGGGGATTCAGGGGCCTTCCAGAGTGTATGGGTTTCGACAGACCATG900.10622351788686014No Hit
ATCTTTGGGAAGATATACAGATGGCAATTCAGCATATGAAAAGATGTCCCAC900.10622351788686014No Hit
CATCACGGGAGATTTTAAAGTTCTAAGTTTTTTGGGAACCTTAAAGGTTGCT900.10622351788686014No Hit
AACTTTGGGTGCCTCATTTGTTTGCTGACATTTATTTTTCATCATTGCAACC890.10504325657700614No Hit
GTATACGGGCAGGAATAATGCATTCCTCTACAGACTCTTTGAAAGGCTTTCT880.10386299526715215No Hit
AAATAGGGAGAGCAGCGTGAACAGGAAGCTAAGCATGAACGCATTGAAAACG880.10386299526715215No Hit
GGTTATGGGAGAATCATGTTTACTCAACATTTTTCCCCAGCTGCCTCAGTAA880.10386299526715215No Hit
CTCATCGGGGAGGAAGGACTGAACCCCTAACAAATCTCTTATCTGAAGGCTC880.10386299526715215No Hit
TTTATAGGGATTAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCG880.10386299526715215No Hit
GATTTAGGGATGATTCAGTAATTGAATGATTATTTAATATATAGTGCTATCA870.10268273395729814No Hit
ACATCTGGGAGACTCACTTGGCACAGGGTTGCCTAGTTTTGCAATTTGTAAA870.10268273395729814No Hit
ATGTGTGGGCAGTCCTCTGCATAAAGCTGAGAGATGCCTACAGCTGAGAGTG870.10268273395729814No Hit
GTACGCGGGATGCTTGTTTTTTCACTTTTTAATTTTTGTCAGTCTGATGGGT870.10268273395729814No Hit
GAATCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA870.10268273395729814No Hit
CGTGCTGGGCTCTGCGGCTGCATTGTGGCCACAGTCAGTTTCTTCTGGGAGG870.10268273395729814No Hit
ACAATGGGGCCCGCCCGGCTGCTCCATGGCGCTGTGCGAGGCCGCGGGCTGC870.10268273395729814No Hit
TCGCTAGGGGGAAAGCAGACTCCTAGGCATCATTGCTGTTTTTTATGAATAG870.10268273395729814No Hit
TAGCTGGAGTGCAGTGGTGCGATCTCAGGTCACTGCAACCTCCGCCTCCTGG870.10268273395729814No Hit
GATTTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA870.10268273395729814No Hit
ATTGAAGGGCGATCTACTTTGGGGCCTGAACTTGGCTCAGCTGCCGGGCTGC870.10268273395729814No Hit
TCCAAGGGGGGCCTTTGCCTTCAAAGAAGGCAATTTATTCTTCTGCATAATT860.10150247264744414No Hit
TACATGGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG860.10150247264744414No Hit
ATCGATGGGGCTTTAAATTCTCCATCTCAGTGAAGGACACCAGGTCTCACCC860.10150247264744414No Hit
GGTACTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC850.10032221133759013No Hit
ACACGTGGGCAACGAAGAGCTAAATAAATGGAGGGATATTCCATGTTTATGG850.10032221133759013No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)