FastQCFastQC Report
Sat 31 Dec 2022
SRR5849252.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849252.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85082
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT5530.6499612138877788No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC5500.646435203685856No Hit
CAAATAGGGGCCAAAAAGTAATGAAAAATTAAAAGGCAAGGAAATTGGGAAA1160.1363390611410169No Hit
AACGAGAGGGGGAGAATGCAGATAATTCACTCATGACTCATTGTTATGGAAG1160.1363390611410169No Hit
GCTTTTGGGCACTAACAAAACAGAATGACAATAAAGAAAACATACCTGGGGA1130.13281305093909404No Hit
TGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG1060.12458569380127407No Hit
GAAACAGGGGATAAAAACATATTTTTAGAAGAAAAAAGATAAATTTAAACCT1040.1222350203333255No Hit
GTCAGGGAGTCAAAGATAGAGCTAAGGAAAGGCAAAAATGAAACTAAATGAA1030.12105968359935122No Hit
AAAATTGGGATGGCCAACTAAAATTGCTTGAAGTAGAAACAGAAAGAAAATC1020.11988434686537693No Hit
TTTATAGGGATTAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCG1020.11988434686537693No Hit
TCCGAAGGGTCCCTGGGAAAAGGCAGTCTTTTCAACAGATGGGAAAAGTGGA970.11400766319550552No Hit
GTGCTCCGGGCAAGACCGAGGCCTGTAGAAGGAAAGCGGAAGGATGTAAGAG970.11400766319550552No Hit
AAGTCTGGGGAAACAATCAACTAAAAACACAACAAAAATGTGTGCAAACCAT960.11283232646153124No Hit
GTAAAAGGGTACCTGTGGATTTATGTCTAAAACCCTACAGGTTTCATTTTTA950.11165698972755694No Hit
CAACAGGGGGATTCAGGGGCCTTCCAGAGTGTATGGGTTTCGACAGACCATG950.11165698972755694No Hit
ACATTAGGGCTCGACGCCTGCCACAACAGTTTTGCCAGTCACCAAACTCAGA950.11165698972755694No Hit
TGTTGCGGGGTGAATACTTTTTTCTTGTTTCAGGCAATTTGAATCAACCCAT930.10930631625960838No Hit
TGATTTGGGTCTGGTCCCTGATCTCGAATATGTAAAGAGCACCTACAAATCA930.10930631625960838No Hit
ATCGATGGGGCTTTAAATTCTCCATCTCAGTGAAGGACACCAGGTCTCACCC930.10930631625960838No Hit
GTTCGCGAGAGAGAAAGAGGAGCGGAAGAAACAGCAGGAGAAGGAAGACAAG920.1081309795256341No Hit
CACATTGGGACAGATTGCTTTACCTGGGTTCTAAAAGTTCTAGTTGGTACTA910.10695564279165981No Hit
GGCGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA910.10695564279165981No Hit
GCCTGGGGTGGAAGACTAAAGCTTTCATTCTAACATTCAGACATAGCAATCC910.10695564279165981No Hit
AACGCCGGGGGCCTGTCAGAAATAGGAAGGTTGTTGATTACTCACAGTTTCA900.10578030605768553No Hit
CCTGAGGAGATGCCTATGAAGTTACATCCTTTTTACAATTAATAAGACAGTT890.10460496932371126No Hit
GAGTTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA880.10342963258973695No Hit
ATCGCATAGGGAGAATACAAAATGACCTTGTTACAGTGTGAGGACATCTTGT880.10342963258973695No Hit
GTAAACGGGTTAAGCACAAATTTGGTAAGTCACATAGAAGAGATGCTCCAGA870.10225429585576266No Hit
TAAACAGGGCATAACACCGGATCAAGTTCGACCCATAGCTTACATTGATTTT860.1010789591217884No Hit
GGTCCAGGGGTGGTACTTAGTGAAAAGTACTACATATTGGTAATCTAATTTA860.1010789591217884No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)