FastQCFastQC Report
Sat 31 Dec 2022
SRR5849248.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849248.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134042
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC9000.671431342415064No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT7460.5565419793795975No Hit
TGGCTAGGGGTACTTGGCATTTAACAACTAGAATTCAGAATCCAGATTTTTA1730.1290640247086734No Hit
CTTTCAAGGGGACACGGTGGGTCCGGCGGCGGGTTGGCGCCCCAGGCGGCGT1670.12458781575923963No Hit
AGACTTGGGCAGCTCACTGAAACCTCTGCCTCCCGGGTTCAAACGATTCTCC1650.12309574610942839No Hit
GAAATGGGTAAGTGAATTCAGCCAGTCATAGAAAGACAAATAGTTTATGATC1620.12085764163471151No Hit
TAAAAGGGGGAGACAGCCTCAGAGCTGGTGGGGAGACAGCATGCCACTGGAA1610.12011160680980587No Hit
ACGTCTGGGGACCAAACCAAAATGGAGTCACTCATGCTAAATGTGACATAAA1600.11936557198490025No Hit
TCACATGGGACCCTGAACAGACCAATAACAAGCAGCGAGATTGAAATGGTAA1600.11936557198490025No Hit
GTGCCAGGGCCGATCTTATGGCGGGTATTGAAATGAAAGAATGACAGAAAGA1540.1148893630354665No Hit
TCTTGCGGGAGATAATATAAGCAAGGAAAACTGTGTTTTCAGAAGCTCACTG1540.1148893630354665No Hit
GTAAACGGGGTAATGAAAGCATCAGGAAACAGAATTCCATTCTTGTTTCATG1530.11414332821056086No Hit
ACTTCCGGGGAAGGTCGCTGGTCATCCTCTGGCACAGAACGAACGTTGTCTT1530.11414332821056086No Hit
GGTCAAGGGCGGCTCACTGCAACCTCCACCTCCCAGGTTCAAGTGATTCTCC1530.11414332821056086No Hit
CATTGTGGGTACCTGTCACTTGTTATCGCTGATTTGTAGCTGTTTCATAAAA1510.11265125856074962No Hit
GTAATAGGGGTGGGTTGAGTGGTGACTCCAAAAAAGATCTGTCCACATCTTA1480.11041315408603274No Hit
TGCGCAGGGAAGAGGAACCAAGAACCTCAGTTCAGGGGAAACACAGCAAGGA1470.10966711926112711No Hit
ACATACGGGACACGTTTAGATTAAAACTAGTTTAAAAAATTATAAATGAATC1460.10892108443622149No Hit
GTTGTTGGGTATCCTGAGAAGAGGGACAGGATTGAGAAGGTTCTGAAATCGT1460.10892108443622149No Hit
GTGGCTGGGAACATGGATCTTAAGGAAAGAAGGAAGATCATCAGGAATAGAA1450.10817504961131585No Hit
TGCACGAGGGAGATCCTAAAAGAAACAAAAAAATTACCTTTGAAGATAGTGA1450.10817504961131585No Hit
AATAGTGGGAACACACATCAGTCCACCCCCATTGTGAATGTTGTCCTGTGTC1440.10742901478641023No Hit
GCTAAAGGGGTTTATAAAAGATTAATAAAATTGATAAACACCTACCAAAACT1440.10742901478641023No Hit
GCATTAGGGGCAAACAATGTTTTCCATAATCTGGAATTGTTGTTTGGAATTC1430.10668297996150461No Hit
ATCACGGGTCAGCCTCAGTAGTAGCGAACTGCACTGCTGTTTTGGTTTGGAG1430.10668297996150461No Hit
GTAGAGGGGGATTTAAGCATTGAAAAAAGCCAAAAGATAATATTAGAAGTTG1420.10593694513659899No Hit
GTAGTGGGGATTTGTAAGATGCTGCACAGGAGCAGCATTATCCCCAAAGATA1410.10519091031169336No Hit
TAAATGGGGAACTAGGAGAAGCTAACAGTGAATGTTTAATTGTGAATTTTAA1410.10519091031169336No Hit
GGCACCGGGGATAAATTGCAGGACTTAACTTACAACCTGTAATTTAAACTAT1410.10519091031169336No Hit
GATACCGGGATCCATGTTGCTGCAAATGAATGACATCATTCTTGTTAATAGC1410.10519091031169336No Hit
GCAATGGGCGAACCGGGTGAACTGAAACATCTCAGTAGCTCGAGGAAAAGAC1410.10519091031169336No Hit
CGTCAAGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGT1400.10444487548678773No Hit
AAGTGAGGGTCATTTCTAAGGTTGCTGCCTTGTCAGAAGAAGCAAACCACAA1390.1036988406618821No Hit
TAGCATGGGCTACCCTATTCAAATAGAAAAGAAATAGCAGACTCCTTTGACA1390.1036988406618821No Hit
CTTAGTGGGATATGCAATTAGAATAAAGCTTCCATTTAACATTTGCCTAATT1390.1036988406618821No Hit
GCTGTTGGGATAAGAGGAAAACAGCAAGAAGTCTGTCTCCTAACTTTGAAAC1360.10146073618716522No Hit
AACGACGGGCAGTTAAAAAAGAAGTAGAAAAGAAACTCCGATGTCTTCTTGC1360.10146073618716522No Hit
TATAGCGGGTGGGGTTTACACTTACCAGTTTCTAATGGAAATTAGAATTCTA1350.1007147013622596No Hit
GTATCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1350.1007147013622596No Hit
GAAACGGGCTTCGATTGAGTCTGAAACAGCAGCTTCTGAGGAAACCTTGTTC1350.1007147013622596No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)