FastQCFastQC Report
Sat 31 Dec 2022
SRR5849247.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849247.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133106
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC9140.6866707736691059No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT7600.5709735098342674No Hit
TGGCTAGGGGTACTTGGCATTTAACAACTAGAATTCAGAATCCAGATTTTTA1690.12696647784472526No Hit
GTAAACGGGGTAATGAAAGCATCAGGAAACAGAATTCCATTCTTGTTTCATG1680.1262151969107328No Hit
TGCACGAGGGAGATCCTAAAAGAAACAAAAAAATTACCTTTGAAGATAGTGA1650.12396135410875542No Hit
GATACCGGGATCCATGTTGCTGCAAATGAATGACATCATTCTTGTTAATAGC1610.12095623037278559No Hit
TCGCAAGGGCAATTCAGACTGTGTTGATTAGCAGATTTATTATTCTATTGAG1580.11870238757080824No Hit
GCAATGGGCGAACCGGGTGAACTGAAACATCTCAGTAGCTCGAGGAAAAGAC1550.11644854476883086No Hit
CCAAGTGGGAAAATACATGAAATGAAATGAAAAACTTAACGTAATTTTCTTC1540.1156972638348384No Hit
GAAATGGGTAAGTGAATTCAGCCAGTCATAGAAAGACAAATAGTTTATGATC1530.11494598290084593No Hit
ACGTCTGGGGACCAAACCAAAATGGAGTCACTCATGCTAAATGTGACATAAA1520.1141947019668535No Hit
TTGCTGGGGGGCCTCGCCAGCCTTTACGAGTTGTCTGCCTCCCTCCGCCACT1520.1141947019668535No Hit
TAGCTGGGGATGGGAAAAATAGGACTTATACTCAGAAACTGAATATTATCAA1510.11344342103286104No Hit
GTAGAGGGGGATTTAAGCATTGAAAAAAGCCAAAAGATAATATTAGAAGTTG1500.11269214009886858No Hit
AGACTTGGGCAGCTCACTGAAACCTCTGCCTCCCGGGTTCAAACGATTCTCC1490.11194085916487612No Hit
ATCACGGGTCAGCCTCAGTAGTAGCGAACTGCACTGCTGTTTTGGTTTGGAG1490.11194085916487612No Hit
GCTAAAGGGGTTTATAAAAGATTAATAAAATTGATAAACACCTACCAAAACT1480.11118957823088366No Hit
AAGTGAGGGTCATTTCTAAGGTTGCTGCCTTGTCAGAAGAAGCAAACCACAA1470.1104382972968912No Hit
GTGGCTGGGAACATGGATCTTAAGGAAAGAAGGAAGATCATCAGGAATAGAA1460.10968701636289874No Hit
AATAGTGGGAACACACATCAGTCCACCCCCATTGTGAATGTTGTCCTGTGTC1460.10968701636289874No Hit
TAGCTGGGGAAGGAGCCTGGGATAGTAACTAGGTTACACTAAAGTTATTTTC1450.10893573542890629No Hit
CATTGTGGGTACCTGTCACTTGTTATCGCTGATTTGTAGCTGTTTCATAAAA1440.10818445449491383No Hit
TCACATGGGACCCTGAACAGACCAATAACAAGCAGCGAGATTGAAATGGTAA1430.10743317356092137No Hit
CTCGATGGGGGGCCCGTCGGGCGGCTCCACAGAGCCGCCCTAGCCTTCCGCC1420.10668189262692891No Hit
ACTTCCGGGGAAGGTCGCTGGTCATCCTCTGGCACAGAACGAACGTTGTCTT1410.10593061169293645No Hit
CGTCAAGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGT1410.10593061169293645No Hit
TGTTTAGGGGAAAAGTAGATTTTGTGATCTACTTTTAGTGGATTTCCTCTCA1400.10517933075894399No Hit
GTACTCGGGAGATTGAATAAAAAACAATAAAAAATATATGCTGTCTACAAGA1400.10517933075894399No Hit
CTTTCAAGGGGACACGGTGGGTCCGGCGGCGGGTTGGCGCCCCAGGCGGCGT1400.10517933075894399No Hit
TAATAGGGGATTTATGAACATGAATTATCTGTGAGTTGGTTAAAGGTAATAT1390.10442804982495155No Hit
GTTGTTGGGTATCCTGAGAAGAGGGACAGGATTGAGAAGGTTCTGAAATCGT1390.10442804982495155No Hit
TCTCATGGGAATCGGACTTATTCACGCAGTGGCCAATAATCAAGACAAACTG1380.10367676889095909No Hit
GCATTTGGGCTATGGCCGGGAGCAGCAAAAGTAACATTTGCCCTATTGTAGG1380.10367676889095909No Hit
ATCAACGGGGTGATCCTCCCAATTCAGTCTCCTGAGTAGCTACAAAACCCAG1380.10367676889095909No Hit
GTGCCAGGGCCGATCTTATGGCGGGTATTGAAATGAAAGAATGACAGAAAGA1380.10367676889095909No Hit
CTATATTTGGGGTGATAATATGGTGTTATGGGAAAACACATTTTGTGCGCAT1370.10292548795696663No Hit
GGTACTGGGATAGTTAAGATTTGGATTTAAGGATCCTATCTTTCTTTATCCC1370.10292548795696663No Hit
GCAACGGGGATTATTGGTTGGGGGAAACCCACGAGGGGACGCGGCCGAGGAG1370.10292548795696663No Hit
TAGCATGGGCTACCCTATTCAAATAGAAAAGAAATAGCAGACTCCTTTGACA1370.10292548795696663No Hit
GGCACCGGGGATAAATTGCAGGACTTAACTTACAACCTGTAATTTAAACTAT1370.10292548795696663No Hit
AACAACGGGAGGTACATCTACATCAGTCATGTCTAACCTGCCACCTCCTGTA1360.10217420702297417No Hit
AACGACGGGCAGTTAAAAAAGAAGTAGAAAAGAAACTCCGATGTCTTCTTGC1360.10217420702297417No Hit
AGACAAGGGGGAGAAAGCGCAGTTCACTGTCATGTTGCAAGTAGATCAAATG1360.10217420702297417No Hit
AATGCTGGGTGTCATGGAAGTGAGAACTCTTCATTACCAAGGAATTGCAAAG1360.10217420702297417No Hit
CCATATGGGAGATAAACAAAATTGATAAACCCTTGGCTAGACTGACCAAGAA1350.10142292608898171No Hit
TAAAAGGGGGAGACAGCCTCAGAGCTGGTGGGGAGACAGCATGCCACTGGAA1350.10142292608898171No Hit
GCGTAGTGGGGTTTCTTATTAGGCCTCAGAAAGAAGTCAGTTAACGTCACCC1350.10142292608898171No Hit
CCTAAAGGGATCTCCGCAAAAATGGAATCATCAAGACAGATAAAGTATTTGA1350.10142292608898171No Hit
TTTATGGGTTGGAACTTCGGGATTTGGTTTCTGAGTCTGTGGAGGCACCGAC1340.10067164515498925No Hit
TAATAAGGGAATAGCTAACAAAATATTTGTGTTTTGATCAAAATTAAAGCAT1340.10067164515498925No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)