FastQCFastQC Report
Sat 31 Dec 2022
SRR5849246.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849246.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133447
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC8320.623468493109624No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT7190.5387906809444948No Hit
GCTAAAGGGGTTTATAAAAGATTAATAAAATTGATAAACACCTACCAAAACT1770.1326369270197157No Hit
GTGCCAGGGCCGATCTTATGGCGGGTATTGAAATGAAAGAATGACAGAAAGA1730.12963948234130404No Hit
TGGCTAGGGGTACTTGGCATTTAACAACTAGAATTCAGAATCCAGATTTTTA1670.12514331532368655No Hit
ACTTCCGGGGAAGGTCGCTGGTCATCCTCTGGCACAGAACGAACGTTGTCTT1650.12364459298448073No Hit
TAGCATGGGCTACCCTATTCAAATAGAAAAGAAATAGCAGACTCCTTTGACA1620.12139650947567199No Hit
TAATAAGGGAATAGCTAACAAAATATTTGTGTTTTGATCAAAATTAAAGCAT1610.12064714830606908No Hit
ACATACGGGACACGTTTAGATTAAAACTAGTTTAAAAAATTATAAATGAATC1590.11914842596686326No Hit
TAGCTGGGGATGGGAAAAATAGGACTTATACTCAGAAACTGAATATTATCAA1580.11839906479726033No Hit
GTTGTTGGGTATCCTGAGAAGAGGGACAGGATTGAGAAGGTTCTGAAATCGT1580.11839906479726033No Hit
TCTTGCGGGAGATAATATAAGCAAGGAAAACTGTGTTTTCAGAAGCTCACTG1570.11764970362765743No Hit
TGCACGAGGGAGATCCTAAAAGAAACAAAAAAATTACCTTTGAAGATAGTGA1560.1169003424580545No Hit
ACGTCTGGGGACCAAACCAAAATGGAGTCACTCATGCTAAATGTGACATAAA1550.1161509812884516No Hit
GGTCAAGGGCGGCTCACTGCAACCTCCACCTCCCAGGTTCAAGTGATTCTCC1520.11390289777964285No Hit
TCACATGGGACCCTGAACAGACCAATAACAAGCAGCGAGATTGAAATGGTAA1490.11165481427083412No Hit
GCCTTCGGGTGAAGCATCCATTCAGAACACGAGGCAATATTGTAGTCCACAG1490.11165481427083412No Hit
TTGCTGGGGGGCCTCGCCAGCCTTTACGAGTTGTCTGCCTCCCTCCGCCACT1490.11165481427083412No Hit
AAGTGAGGGTCATTTCTAAGGTTGCTGCCTTGTCAGAAGAAGCAAACCACAA1450.10865736959242246No Hit
AAGTCTGGGGGTTGAGAAGGAGATGGAAGAGAAGGGGTTTGTCTTGCTGTCT1450.10865736959242246No Hit
GAAATGGGTAAGTGAATTCAGCCAGTCATAGAAAGACAAATAGTTTATGATC1450.10865736959242246No Hit
GATACCGGGATCCATGTTGCTGCAAATGAATGACATCATTCTTGTTAATAGC1450.10865736959242246No Hit
CATTGTGGGTACCTGTCACTTGTTATCGCTGATTTGTAGCTGTTTCATAAAA1440.10790800842281954No Hit
GGTTCTGGGGTTGGAATGTTGCTGTCATTACGAAATGCCACTCTGGACGTAT1430.10715864725321664No Hit
TAAATGGGGAACTAGGAGAAGCTAACAGTGAATGTTTAATTGTGAATTTTAA1430.10715864725321664No Hit
CCTGTTGGGATGGTTAAAACTGCTTTGAATCAAATTAACCCAAGATTTAGGT1430.10715864725321664No Hit
AGACAAGGGGGAGAAAGCGCAGTTCACTGTCATGTTGCAAGTAGATCAAATG1420.10640928608361372No Hit
GTTACAGGGATATGAAAGCTGGAAATTAAAAAATTGGTATCGTTAAGATACA1410.10565992491401081No Hit
TAGCTGGGGAAGGAGCCTGGGATAGTAACTAGGTTACACTAAAGTTATTTTC1410.10565992491401081No Hit
GCAACGGGGATTATTGGTTGGGGGAAACCCACGAGGGGACGCGGCCGAGGAG1400.1049105637444079No Hit
GTAAACGGGGTAATGAAAGCATCAGGAAACAGAATTCCATTCTTGTTTCATG1390.10416120257480499No Hit
AACAACGGGAGGTACATCTACATCAGTCATGTCTAACCTGCCACCTCCTGTA1390.10416120257480499No Hit
GTTCTGGGTACATGAATATTTTACCACGAAATTGCCATATAATATCTTGTCT1380.10341184140520207No Hit
GTCAAAGGGGTAAATTGGGTTGTGGTTTGGGGTCAGTTTTTAAATGAAATAG1370.10266248023559917No Hit
GCGTAGTGGGGTTTCTTATTAGGCCTCAGAAAGAAGTCAGTTAACGTCACCC1370.10266248023559917No Hit
TCACCAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1360.10191311906599623No Hit
CCATATGGGAGATAAACAAAATTGATAAACCCTTGGCTAGACTGACCAAGAA1350.10116375789639334No Hit
GGTGGCGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTG1350.10116375789639334No Hit
TAGTTAGGGATTGTACCTTGATTATGCCTGTTGGTAACCCAGGTTAATAAAT1350.10116375789639334No Hit
GCATTAGGGGCAAACAATGTTTTCCATAATCTGGAATTGTTGTTTGGAATTC1340.10041439672679041No Hit
TTCGGGGGAGATAGAGAAAAGAAAAAGAGCTTCCTTTACTTGCTTGTCTTCT1340.10041439672679041No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)