FastQCFastQC Report
Sat 31 Dec 2022
SRR5849235.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849235.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171392
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT9780.5706217326362957No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC8470.49418876026885733No Hit
TATTCCGGGGATTTCAGAAAACCTTTGATAACTAATTGAGAAAATGCAGAAG2360.13769604182225542No Hit
AAATATGGGTATTAATGTTTGATAAAGGTATATCCCAGTTAACTACACTGCT2230.13011109036594473No Hit
GTCACCGGGAGAATGAGAAAGTTCACATTCCTCACTTCCATCTACCCATGCC2040.11902539208364452No Hit
GAGCGGGGGAGAACATATTTTCCTCCACCTGCCTTTTAAGTTTTTATTTCAG1920.11202389843166542No Hit
TAGGCTGGGAGGGCAACCTGGGGCAGACAGGCCTGGTGGGGGGTGGGGAAAC1870.10910660941000747No Hit
TCACAAGGGCGACTGAATTAGAAATGGAAATGCCTGGCACAGCCAGGAAGGA1850.10793969380134427No Hit
TCGTGCGGGCCGATCTAAGGCAGGGCTGGATATGTGTTCTGTTTCTAAGCGA1840.1073562359970127No Hit
AATTCGGGAACAAGGCATGGAAAGTGGTGAATATAAAAAACACAAGATCTCT1840.1073562359970127No Hit
TATTGTGGGAAGAAAAGAAAGGAAAAGTGAAGTAGGCATATGAAAGAAAAGG1780.10385548917102316No Hit
TGTCGAGGGATCCTCTCATTTTCCAGTTTAGAAGGTGCTTTCAGATATTTAG1740.10152165795369679No Hit
CGAATCGGGCTGGGATTCTACAAGTGACAGTTGTCTGTTTACTCTTGAAGTT1740.10152165795369679No Hit
GTTACTGGGATTTTTCTTGAATCTAATTGTAGATGATAGGCTTATTCAAAAC1730.1009382001493652No Hit
CCAGAAGGGAAGAGCTTTCTCAACAAATTAGTCCATCTCATTGATGTACCAC1720.10035474234503361No Hit
TAGCGAGGGGAAGCGAAATCGAGGACACTGATGATTCTTCCAAGCCCGCAGG1720.10035474234503361No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)