FastQCFastQC Report
Sat 31 Dec 2022
SRR5849234.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849234.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171862
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT9200.5353132164178236No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC8610.5009833471040719No Hit
ACAATTGGGGTTTTTAAGTTTTATCATCCATACTGAATTGAGAGATATGCTC2010.11695430054345927No Hit
TACACGGGAGTTTCAGAATAAAAATATTGACAGTATATTGAATTTCTGAATT1900.11055381643411576No Hit
AGCTAAGGGAAAACAAAACATTACAATTATTGCTTCTTTTTATTTGTCTAAG1800.10473519451653071No Hit
TGAACTGGGACTAAATTTCCTTCTTAAATTATTTAATTCATCCAGTAGTTAT1790.10415333232477221No Hit
TTGCTGGGGGGACTTTGAAACAAATGCAATGAATGGCATCCACTAGTTTCAA1790.10415333232477221No Hit
CTTCGCGGGCACTAAACTCACTACTTTTAAATTTCATGTTTCCTTAGATCTC1780.10357147013301371No Hit
TAGTGTGGGAGATGAGTGTTGCCCTCTTCACATTTTTTATCTTAGCTCAACT1770.10298960794125521No Hit
TGACTTGGGGAGGAGAGAGAGCTTGAAAAGCTGTTTCAGCTGGGCCCCCCTT1770.10298960794125521No Hit
TGCTAAGGGGATTCATTGTCTTTCCTTCCTTCATGCATGAATGAGTATAACA1760.10240774574949668No Hit
TGTCGAGGGATCCTCTCATTTTCCAGTTTAGAAGGTGCTTTCAGATATTTAG1750.10182588355773818No Hit
TAGGCCGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG1750.10182588355773818No Hit
TCGGGTGGGGCTCCGGAAGGAGACGTGGCGGCGGTTGGGCCGGTGATACCCG1730.10066215917422117No Hit
GTAACGCGGGTATAAGCATTTTCTGATTCTATAAATGATTACTACCACAAAT1720.10008029698246267No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)