FastQCFastQC Report
Sat 31 Dec 2022
SRR5849224.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849224.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102734
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT10871.0580723032297001No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC10531.0249771253917885No Hit
AATTCTGGGATAGGGACTAATATTTCCTTTTCTTTTTTTTAAAAAAAACATT1580.15379523818794166No Hit
AGCCGCGGGACCTGGGACCTGATTTATTGAAATTTTTCTCTTTAAAAACATT1470.14308797476979385No Hit
TACCATCGGGCCACCCTTTGATCTGGAATGATAAAGATGATAAGGGTGGGAT1430.1391944244359219No Hit
GATACGGGAACACTCATCAGGCTTCACTATTCTGTTTTCTAGGCCTGTTACA1410.13724764926898592No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCCTGTCTCTTATAC1380.13432748651858198No Hit
CTATCAAGGGGATTGTGAAAATGACCATACTGCCAAAAGCAATCTACAAATT1380.13432748651858198No Hit
TTTTCTGGGAATTGAAAAAAGAAGGAGTCAAAATATTAAAATGGTACAGATC1380.13432748651858198No Hit
TCCCATGGGAGATTCTGCTAGCCTGTTCTTTTACCACTGACAGCTAGACACC1360.13238071135164597No Hit
GTAAATGGGATCTTCAAAGGATTGGGGTGCAAAAATACCTTGCTGGTAAGCC1360.13238071135164597No Hit
TGCTCTGGGGATCCGGCGCCGGCTTTCGGCGCGACGGTCGCCGCGTTCCATC1350.131407323768178No Hit
TACCTTGGGCATTAAAAGCAGAAACTGGTAGCAGAAGAGATTTACAGATTTT1330.12946054860124206No Hit
TTTGCCGGGGTAGTACCAACAAGTACCATTCGTATTATGATTCACAATTTAC1300.1265403858508381No Hit
ATTACCGGGGAATTTCTTTCTAGCAATTGCAGAGCCTGTTTTTGCTGTGGTC1280.12459361068390211No Hit
CAATCCGGGAAGTCAAAATATGACTCAAGTGTGAGTGGTCAATCATCTTTTG1270.12362022310043413No Hit
CGACTTGGGCCCCTCGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCA1270.12362022310043413No Hit
GTGACTGGGGAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTT1260.12264683551696613No Hit
TGAGTAGGGTATCTCAAGTTGTTTTCATTTTCAGTATTGAGTTCTCCCTTAC1260.12264683551696613No Hit
GGTAGTGGGATTTGCTCTCCATTAGCAAAATAATTCATTGTTAGGTAAACTT1260.12264683551696613No Hit
CCCAGGCTCAAGCGATCCTTCCACCCCAGCCTCTCGAATAGCTGGGACTACA1250.12167344793349816No Hit
GAATTGGGGCTGGATATGGGATTATCAAAGGTATGATTAGAAACAAATGGGA1250.12167344793349816No Hit
CTTCAGGGGCCCATGTAACTTAGGTAGAAAAAACTGGAGAAACTAAATTGTT1240.12070006035003018No Hit
GCTTGGGAGCTATTTTTACCAAGTATTTTGTAAATGCTAATTTTTTAGGACT1230.1197266727665622No Hit
TCAGCAGGGCTTCTTTACGAAGAGTGAAATATGTTTTTGTACCTCTCAGTTT1230.1197266727665622No Hit
GCGCTAGGGATGTTGGAAATAGAAAACAGATGTTTTAATGGGGTGATACCCA1220.11875328518309419No Hit
CAGTCCGGGCAGCCATAAAAAATATGTGAATGAATGAATGTGGCTGTATGCC1220.11875328518309419No Hit
TACAGACGGGGATATCCTTGTTTTTAACCTTGTGGCTTGCTGAATCAAAGCC1210.11777989759962622No Hit
GCGATGGGTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTA1210.11777989759962622No Hit
TTTTGAGGGGCATTATTAAGGAAGGGAAAGTTGCTATAATAATAAAAGGTTG1200.11680651001615824No Hit
TCAGCAGGGGAATTTGTGAAGACTGGTACTATGATTTTTTTCCTTATATATT1200.11680651001615824No Hit
TTTGCAGGGAAGCCTGGCCAACTTGGCAAAACCCCGTCTCTACTAAAAATAC1190.11583312243269024No Hit
TGTTTGGGGAAAACCTTTGGATTCAATACACACAAATACATTATATCTAAGA1180.11485973484922227No Hit
AGGTACGGGGAGGATGAAGAGGGAAATGCCAAACTGCAGCAGCGCCAGCTCA1180.11485973484922227No Hit
CCCCACCATCACTATACAAATAAAAAGAAATCAATAGGCCCAGAAACATAAG1180.11485973484922227No Hit
TCTCGGGGGCATAGGCATTCTCGGGACCGTCCTTATCCCATTTAATTAATTT1180.11485973484922227No Hit
GACTTTGGGCACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTC1170.11388634726575428No Hit
TCTATAGGGCAATATTTGTATCTTATCAGTACAGAAGAAATATTTCTGCGTA1160.1129129596822863No Hit
GCGTAAGGGATGCTAGATCTTGACATGCCCTCAACTCAGTGGTACTTCTTTA1160.1129129596822863No Hit
CTAGACGGGGCAATTAGCCTGTAGATAAGTTTTGTGGAAATTGCCACCTTAA1160.1129129596822863No Hit
GCTTACGGGCCGGGACAGCGTTCGCCCTTTAGCGGGGAGGTCATTACAGCCT1150.1119395720988183No Hit
CATAAAGGGTAGCGATTGGTTTCTGGTAAAACAAGTCCTGCCAGATTCCTAT1150.1119395720988183No Hit
AGCCTGGGCGACAGAGCGAGACTCCGTCTCAATAATCATCATCATCATCATC1150.1119395720988183No Hit
GTGTGTGCACCACCCCCTCACACACACTCACATGCACACACACAGGAAGTAA1150.1119395720988183No Hit
GGCATGTGGGATTGAAGTAATAACCAAATAATTTTCAATCTTGATTCAACTG1150.1119395720988183No Hit
ATTGCTGGGTATTCAAGTATTTGATCAGTAAGTCACAGTTATCTCCAGTGCA1140.11096618451535033No Hit
TGGCTATGGGAGAGTTTCTAACCTAATTTGTAATGCTGTAATCACATACTTA1140.11096618451535033No Hit
GAAACTGGGATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTG1140.11096618451535033No Hit
TCAAAAGGGCTCACCAAGATGAACTATTTGCCGCCCTGTCTTTTCCTGGGTT1130.10999279693188234No Hit
TAGCTGGGGGTAGGAACTTTTAGTTCCATTATATGAACACTGGATAACCTAA1130.10999279693188234No Hit
TGTGGCTGGGGATTGTTGGGCTATTTGCATGATTTCCTAAAATATCTGGCAA1130.10999279693188234No Hit
ATTAACGGGGTATAGGCTATGGTGAGTAAGCCAAGAGACCAACATGAGTTTG1110.10804602176494636No Hit
CTTCGGGGAAACAAAACAAAAAAAGAGTATTCATTGTGAGGACAAACAATAC1110.10804602176494636No Hit
AACTAAGGGCTGTGGAACAGTAATTTGTGCCCAATTTAGTGGAAGATAATGA1100.10707263418147839No Hit
GCTTTGGGGATATTTGAAGCGTAGACAAAATTCTGTTGGCTTTTAAAAGAAA1100.10707263418147839No Hit
TCTATGGGTTGGTGGTTATTGGAACCACACTGGCAGGGCTGACATTGATTCT1100.10707263418147839No Hit
ATCAAAGGGCGATCTGATGCGGGGTGGGTGAGAATGATTACATCAGAGAACT1100.10707263418147839No Hit
TGAACTGGGAGGGCCTCCCGCATCAAGGTCAGCATGAGGACAGACCACAGAG1090.10609924659801039No Hit
TGCGCAAGGGTACGAGTGAGATCATAACTTCTGATGTAAGTATATAAGGTAT1090.10609924659801039No Hit
CTCTTAGGGATTCTTCAACCACCTCGGTTACTCTGACATAGGAATTTACTTC1090.10609924659801039No Hit
TGAGATGGGGAAAGTGTTTTGCAAAATTGAAAGTGCTGTACAAATACAGGAT1090.10609924659801039No Hit
GTTAGTGGGTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAG1080.10512585901454241No Hit
ACAGTGGGCAACATTCAGTATCAGAAGAAAATATAAATTAAAACCACAATGA1080.10512585901454241No Hit
GGGCATGGGCACAGGGCAAACCACTTCTCCTCAAATTAGAGAAATAGGCAAA1080.10512585901454241No Hit
AATTAGGGGATCTCTTATGGGGGTTATCACTCTTAGGTCAGACAGCCATCAG1070.10415247143107442No Hit
TTTGATGGGAGCTCGAACAGTCTCAGATCTGTTTAATTTATGCTAATGATCA1070.10415247143107442No Hit
TCTTCGGGGATTGTTCAGCAGTTTCAGAGGAAATATCCCAATTTATCTTGTG1070.10415247143107442No Hit
GCCAAAGGGCTTCCACAGAGGAAATTGACAGGATGGTCATAGATCTGGAAAA1070.10415247143107442No Hit
CGCGTGGGGAATTGTTAGAAAGCAGATACCATCAAATTGGTTCTGGGAAGTG1070.10415247143107442No Hit
TTATTTGGGTGGGCATGTTTACTTTGAGTTCCACATGGGATAAGAACAGGGA1060.10317908384760645No Hit
GAACTTGGGGCCCTCTGTACTTCCCATGGTGATTTTGAGGAGCACATGCATA1060.10317908384760645No Hit
GTCTATGGGAAACCAAAGTGTTCCCCCCAACCCCAAATTTGTCTAAGCACTG1060.10317908384760645No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTT1060.10317908384760645No Hit
TATTATGGGATGGGGAATGAGATTCTTGGAGATCCCACCTGATCTTTACTAA1050.10220569626413845No Hit
TATCCTGGGCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTTGGA1050.10220569626413845No Hit
GCAGTTGGGGAAACAGACTCTGACTTTATTCCTCTTTTAGTTCGAGCCTTCA1050.10220569626413845No Hit
TGCTAGGGGGCAGGGCGAAAGGAGGCTGTGTCCTCAGCCTCCTCCCTCGGCA1050.10220569626413845No Hit
ACAAGTGGGATGGATTCTTTTAAATCTCTTAAAAAACGGTAGTGTCTTTCAA1050.10220569626413845No Hit
TTAGACGGGAATCTCTTATTACTTAAAATTGAGAAATATCAAAATGACCTAT1050.10220569626413845No Hit
TTTGCAGGGAATCAAAGGATGGTGCTGGTTAATTTGTCAAACCCATATTTGC1050.10220569626413845No Hit
GCAAACGGGAAGTGAATCTCATAGATATCTACAGAACTCTCCAACACGAAAC1050.10220569626413845No Hit
GTTAATGGGATTGTTGCAAGAGAACATCCATTCCCATCCATTAGGCCAGGGC1040.10123230868067047No Hit
GTGTTCGGGAAGCACCAGGCACAGATTGACCAATATCTGGGACTTGTGAGGA1040.10123230868067047No Hit
ACTTGTGGGTGTTGCTGCTGTCCCCAGTGATGGTGGACTTATCTTTGCCTTA1040.10123230868067047No Hit
CGCTTTGGGGTAGGTCTTCAATAATGTTTGTTTGCTTAATTACTAAATTGAA1040.10123230868067047No Hit
TGTACGGGGAGCTACATAATTTTTTTTGCTTTTTGCAAATCTTTAATTATTT1040.10123230868067047No Hit
AGTCTTGGGGTCACCGTATGCGTGAAATTATATATTTCGGGGTAGTGTGAGC1040.10123230868067047No Hit
AAAGCTGGGCAATGACTAAGGGGAAAATTATACTGTCACTATCATTTGACTT1030.1002589210972025No Hit
CGAGTGGGGTATGTGCTCAGTAAATATTTGTTCAATGAATGAATGAATGAAT1030.1002589210972025No Hit
TTCGTAGGGGTAGTTGCAGCTGATGCCTGAATGTATCCTAGCTGACAAATTA1030.1002589210972025No Hit
TCTCTCGGGGTAGACATATTTTTGCTCTCCTGATTTGCAGTAAGATTTTAAA1030.1002589210972025No Hit
GGCATCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1030.1002589210972025No Hit
GATGCAGGGAGGATGGCTTTGTTAATGTCAAATGAAGTAGATCTTAGACAAG1030.1002589210972025No Hit

[WARN]Adapter Content

Adapter graph