FastQCFastQC Report
Sat 31 Dec 2022
SRR5849223.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849223.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103382
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT10591.0243562709175678No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC10060.9730900930529492No Hit
TTTGATGGGAGCTCGAACAGTCTCAGATCTGTTTAATTTATGCTAATGATCA1590.15379853359385579No Hit
AATTCTGGGATAGGGACTAATATTTCCTTTTCTTTTTTTTAAAAAAAACATT1570.15186396084424755No Hit
TTAGACGGGAATCTCTTATTACTTAAAATTGAGAAATATCAAAATGACCTAT1430.13832195159698982No Hit
AGCCGCGGGACCTGGGACCTGATTTATTGAAATTTTTCTCTTTAAAAACATT1430.13832195159698982No Hit
TCAAAAGGGCTCACCAAGATGAACTATTTGCCGCCCTGTCTTTTCCTGGGTT1420.13735466522218567No Hit
TGAGTAGGGTATCTCAAGTTGTTTTCATTTTCAGTATTGAGTTCTCCCTTAC1420.13735466522218567No Hit
GTAAATGGGATCTTCAAAGGATTGGGGTGCAAAAATACCTTGCTGGTAAGCC1420.13735466522218567No Hit
CGCTTTGGGGTAGGTCTTCAATAATGTTTGTTTGCTTAATTACTAAATTGAA1390.1344528060977733No Hit
TTATTTGGGTGGGCATGTTTACTTTGAGTTCCACATGGGATAAGAACAGGGA1350.1305836605985568No Hit
AGGTACGGGGAGGATGAAGAGGGAAATGCCAAACTGCAGCAGCGCCAGCTCA1340.1296163742237527No Hit
TTGTACGGGGAGGTTGAAGAATGAGACCCTTAGGTTTTAACACGAATCCTGA1340.1296163742237527No Hit
ACAGTGGGCAACATTCAGTATCAGAAGAAAATATAAATTAAAACCACAATGA1340.1296163742237527No Hit
CTATCAAGGGGATTGTGAAAATGACCATACTGCCAAAAGCAATCTACAAATT1330.12864908784894855No Hit
GATACGGGAACACTCATCAGGCTTCACTATTCTGTTTTCTAGGCCTGTTACA1320.12768180147414443No Hit
TACCATCGGGCCACCCTTTGATCTGGAATGATAAAGATGATAAGGGTGGGAT1310.1267145150993403No Hit
TTTTCTGGGAATTGAAAAAAGAAGGAGTCAAAATATTAAAATGGTACAGATC1290.12477994234973207No Hit
ATATCAGGGACATTAAGAAACCGCACGTGATGTCGCTATGCTCTAACGATCA1290.12477994234973207No Hit
TCCCATGGGAGATTCTGCTAGCCTGTTCTTTTACCACTGACAGCTAGACACC1280.12381265597492795No Hit
CAATCCGGGAAGTCAAAATATGACTCAAGTGTGAGTGGTCAATCATCTTTTG1270.12284536960012382No Hit
TGCTCTGGGGATCCGGCGCCGGCTTTCGGCGCGACGGTCGCCGCGTTCCATC1270.12284536960012382No Hit
GGCATCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1270.12284536960012382No Hit
GAATTGGGGCTGGATATGGGATTATCAAAGGTATGATTAGAAACAAATGGGA1270.12284536960012382No Hit
ATTGCTGGGTATTCAAGTATTTGATCAGTAAGTCACAGTTATCTCCAGTGCA1260.12187808322531969No Hit
TGGCTATGGGAGAGTTTCTAACCTAATTTGTAATGCTGTAATCACATACTTA1260.12187808322531969No Hit
TTTAATGGGGACGTCCGGGGTGAAAGGGCGCAGCCGCCGGAAGGGCCCGGGG1250.12091079685051558No Hit
TACCTTGGGCATTAAAAGCAGAAACTGGTAGCAGAAGAGATTTACAGATTTT1250.12091079685051558No Hit
GAAACTGGGATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTG1250.12091079685051558No Hit
GCTTTGGGGATATTTGAAGCGTAGACAAAATTCTGTTGGCTTTTAAAAGAAA1250.12091079685051558No Hit
AGCCTGGGCGACAGAGCGAGACTCCGTCTCAATAATCATCATCATCATCATC1240.11994351047571145No Hit
TTTTGAGGGGCATTATTAAGGAAGGGAAAGTTGCTATAATAATAAAAGGTTG1230.11897622410090732No Hit
CATAAAGGGTAGCGATTGGTTTCTGGTAAAACAAGTCCTGCCAGATTCCTAT1230.11897622410090732No Hit
GGCATGTGGGATTGAAGTAATAACCAAATAATTTTCAATCTTGATTCAACTG1230.11897622410090732No Hit
CTGCGAGGGATCACACCAGGCTATGGCAATCATGATGCTATGCCATTTCTAA1220.11800893772610319No Hit
GTTAATGGGATTGTTGCAAGAGAACATCCATTCCCATCCATTAGGCCAGGGC1210.11704165135129907No Hit
AACTAAGGGCTGTGGAACAGTAATTTGTGCCCAATTTAGTGGAAGATAATGA1200.11607436497649494No Hit
GACTTTGGGCACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTC1200.11607436497649494No Hit
AGTCTTGGGGTCACCGTATGCGTGAAATTATATATTTCGGGGTAGTGTGAGC1190.11510707860169081No Hit
TGTTTGGGGAAAACCTTTGGATTCAATACACACAAATACATTATATCTAAGA1180.1141397922268867No Hit
TCTATAGGGCAATATTTGTATCTTATCAGTACAGAAGAAATATTTCTGCGTA1180.1141397922268867No Hit
TGTGGCTGGGGATTGTTGGGCTATTTGCATGATTTCCTAAAATATCTGGCAA1180.1141397922268867No Hit
GCGTAAAGGATTTGGCAAAATTGAAAACTTTTTTCATGATTTATAAAAACAA1180.1141397922268867No Hit
TTTGCAGGGAATCAAAGGATGGTGCTGGTTAATTTGTCAAACCCATATTTGC1180.1141397922268867No Hit
TCTCTCGGGGTAGACATATTTTTGCTCTCCTGATTTGCAGTAAGATTTTAAA1170.11317250585208256No Hit
GCCAAAGGGCTTCCACAGAGGAAATTGACAGGATGGTCATAGATCTGGAAAA1170.11317250585208256No Hit
AATGATGGGTAGGATATTTTGTTACATATTTGTAAATTTTTGAAATGCTAGT1170.11317250585208256No Hit
ACAATGGGGTATTTTGACAAGTAGCATTCAAGAAAGGGTTTTCAGTGAGCTG1170.11317250585208256No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCCTGTCTCTTATAC1160.11220521947727843No Hit
TTCGTAGGGGTAGTTGCAGCTGATGCCTGAATGTATCCTAGCTGACAAATTA1160.11220521947727843No Hit
ATCAAAGGGCGATCTGATGCGGGGTGGGTGAGAATGATTACATCAGAGAACT1160.11220521947727843No Hit
GGCACAGGGTATTGTAACTTACTACTCCGGAAAAAAAGAACCATTTGGATAC1150.11123793310247432No Hit
CCGACGGGGCACTCACCTTGGCCTCCCAAAGTGCTAGGATTACAGGTGTGAG1150.11123793310247432No Hit
TTTGCCGGGGTAGTACCAACAAGTACCATTCGTATTATGATTCACAATTTAC1150.11123793310247432No Hit
TGAACTGGGAGGGCCTCCCGCATCAAGGTCAGCATGAGGACAGACCACAGAG1140.11027064672767019No Hit
CCCAGGCTCAAGCGATCCTTCCACCCCAGCCTCTCGAATAGCTGGGACTACA1140.11027064672767019No Hit
TGTTCAGGGTCTCAGTGGAAATCTTAAAAGACAGGAGAGATTGGCATGACAT1140.11027064672767019No Hit
CGACTTGGGCCCCTCGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCA1140.11027064672767019No Hit
CTAAGTGGGCAACTTTTTTCCTGTGAACACATAAATATATTTTTATAGAAAA1130.10930336035286606No Hit
CCCCACCATCACTATACAAATAAAAAGAAATCAATAGGCCCAGAAACATAAG1130.10930336035286606No Hit
TAGCTGGGGGTAGGAACTTTTAGTTCCATTATATGAACACTGGATAACCTAA1130.10930336035286606No Hit
ACTCATCGGGACCTATTCTGTAGTTGCAGCTGATGCCTGAATGTATCCTAGC1120.10833607397806194No Hit
CTTGTGACGGGGTATCAATAGCCTACAGAAGAAAAATTATATTATTATATCA1120.10833607397806194No Hit
GTGTGTGCACCACCCCCTCACACACACTCACATGCACACACACAGGAAGTAA1110.10736878760325781No Hit
CTGTCAGGGGGAGTTGAAATGAGATGAAATGATGAAATGATGAGATGAAATG1110.10736878760325781No Hit
GCGCTAGGGATGTTGGAAATAGAAAACAGATGTTTTAATGGGGTGATACCCA1110.10736878760325781No Hit
TGAAGTGGGCAAAACGAAGTGAAGTGAAAATTTAATCAACTGTGTAGACTGT1110.10736878760325781No Hit
TGCGTTAGGGCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCA1100.10640150122845368No Hit
AATTAGGGGATCTCTTATGGGGGTTATCACTCTTAGGTCAGACAGCCATCAG1100.10640150122845368No Hit
CCTGAATGGAGAGTCATGGCCCAGCAGCATTTACCACAAGGTGACTGAAGTA1100.10640150122845368No Hit
AGCCACGGGTAAAATAAAATTATTGTTTTTACATATAAAGATGATTTTTCAC1090.10543421485364958No Hit
ATATGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1090.10543421485364958No Hit
AAAGCAGGGGTGATTGCTAATCAAAATTCTAACATTATACAACCTTTTCCCC1090.10543421485364958No Hit
TATTATGGGATGGGGAATGAGATTCTTGGAGATCCCACCTGATCTTTACTAA1080.10446692847884545No Hit
TTGGGTGGGTAGTAAGTTTATTTTTGCAAATATATGCATGTATCATAGAAAA1080.10446692847884545No Hit
TCTCGGGGGCATAGGCATTCTCGGGACCGTCCTTATCCCATTTAATTAATTT1080.10446692847884545No Hit
GCAGTTGGGGAAACAGACTCTGACTTTATTCCTCTTTTAGTTCGAGCCTTCA1080.10446692847884545No Hit
GTCTATGGGAAACCAAAGTGTTCCCCCCAACCCCAAATTTGTCTAAGCACTG1080.10446692847884545No Hit
GCAGTTGGGGTGTTCCTTTTTAACAAGGAAAAACAGAAAATAATGGAATTCC1080.10446692847884545No Hit
GCGATGGGTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTA1080.10446692847884545No Hit
TGTTATGGGGGTTCAAGATGAAGCAAACTAAAATGAGGCATGATTCTAAGGA1070.10349964210404133No Hit
GGTAGTGGGATTTGCTCTCCATTAGCAAAATAATTCATTGTTAGGTAAACTT1070.10349964210404133No Hit
AGATACGGGCTTTCTCTAGACAAAGACAAGCTCCTACGGCTGCTCCTCCTGG1070.10349964210404133No Hit
CCATAGGGGATTTTTAGTGTTAGTAAAATCGTTAAAATTTATATCCAGTGAA1070.10349964210404133No Hit
GCGCTGGGATTTGAGGAAATAGGACGCCGCCTAAAGGATATTGGGCCTGAGG1060.1025323557292372No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCCTGTCTCT1060.1025323557292372No Hit
GTTTCAGGGAGAAATTAATAGCACTAAATGCCCACATCAAAAAGCTAGAAAG1060.1025323557292372No Hit
AATCCAGGGCCCTGCGGGAGTCAATGATCCAGCTAGCTGAGGGGCTTATCAC1060.1025323557292372No Hit
CTTTGGGGAAGCCTTTCTCAAGTAGAGGTTATTCTTCAGCATAGTGCTGCTG1060.1025323557292372No Hit
TAGCTGGGATCCCCAGGACGGTGGGCACTGGCCTGGCCAAAACAAGGAACAG1050.10156506935443307No Hit
ACTTGTGGGTGTTGCTGCTGTCCCCAGTGATGGTGGACTTATCTTTGCCTTA1040.10059778297962896No Hit
TGCTAGGGGGCAGGGCGAAAGGAGGCTGTGTCCTCAGCCTCCTCCCTCGGCA1040.10059778297962896No Hit
CTTCAGGGGCCCATGTAACTTAGGTAGAAAAAACTGGAGAAACTAAATTGTT1040.10059778297962896No Hit
CGCGTGGGGAATTGTTAGAAAGCAGATACCATCAAATTGGTTCTGGGAAGTG1040.10059778297962896No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTT1040.10059778297962896No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)