FastQCFastQC Report
Sat 31 Dec 2022
SRR5849222.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849222.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104692
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT10511.003897145913728No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC9070.8663508195468612No Hit
AATTCTGGGATAGGGACTAATATTTCCTTTTCTTTTTTTTAAAAAAAACATT1570.1499637030527643No Hit
GATACGGGAACACTCATCAGGCTTCACTATTCTGTTTTCTAGGCCTGTTACA1530.14614297176479576No Hit
ACAATGGGGTATTTTGACAAGTAGCATTCAAGAAAGGGTTTTCAGTGAGCTG1490.1423222404768273No Hit
TTTGCCGGGGTAGTACCAACAAGTACCATTCGTATTATGATTCACAATTTAC1480.14136705765483512No Hit
TGCTCTGGGGATCCGGCGCCGGCTTTCGGCGCGACGGTCGCCGCGTTCCATC1450.13850150918885876No Hit
TGAGTAGGGTATCTCAAGTTGTTTTCATTTTCAGTATTGAGTTCTCCCTTAC1410.13468077790089023No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCCTGTCTCTTATAC1380.13181522943491383No Hit
AGCCGCGGGACCTGGGACCTGATTTATTGAAATTTTTCTCTTTAAAAACATT1370.13086004661292172No Hit
CATAAAGGGTAGCGATTGGTTTCTGGTAAAACAAGTCCTGCCAGATTCCTAT1360.12990486379092958No Hit
GAAACTGGGATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTG1360.12990486379092958No Hit
CTATCAAGGGGATTGTGAAAATGACCATACTGCCAAAAGCAATCTACAAATT1350.12894968096893747No Hit
TTTTGAGGGGCATTATTAAGGAAGGGAAAGTTGCTATAATAATAAAAGGTTG1320.12608413250296105No Hit
GAATTGGGGCTGGATATGGGATTATCAAAGGTATGATTAGAAACAAATGGGA1320.12608413250296105No Hit
AACTAAGGGCTGTGGAACAGTAATTTGTGCCCAATTTAGTGGAAGATAATGA1310.12512894968096894No Hit
GGCACAGGGTATTGTAACTTACTACTCCGGAAAAAAAGAACCATTTGGATAC1310.12512894968096894No Hit
TGTTATGGGGGTTCAAGATGAAGCAAACTAAAATGAGGCATGATTCTAAGGA1270.12130821839300042No Hit
TTTGATGGGAGCTCGAACAGTCTCAGATCTGTTTAATTTATGCTAATGATCA1270.12130821839300042No Hit
GCTTTGGGGATATTTGAAGCGTAGACAAAATTCTGTTGGCTTTTAAAAGAAA1260.1203530355710083No Hit
TTAGACGGGAATCTCTTATTACTTAAAATTGAGAAATATCAAAATGACCTAT1260.1203530355710083No Hit
CTTCGGGGAAACAAAACAAAAAAAGAGTATTCATTGTGAGGACAAACAATAC1250.11939785274901617No Hit
CGACTTGGGCCCCTCGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCA1240.11844266992702404No Hit
TCTATAGGGCAATATTTGTATCTTATCAGTACAGAAGAAATATTTCTGCGTA1230.11748748710503192No Hit
TATCCTGGGCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTTGGA1230.11748748710503192No Hit
GTAAATGGGATCTTCAAAGGATTGGGGTGCAAAAATACCTTGCTGGTAAGCC1230.11748748710503192No Hit
AGTCTTGGGGTCACCGTATGCGTGAAATTATATATTTCGGGGTAGTGTGAGC1230.11748748710503192No Hit
TACCATCGGGCCACCCTTTGATCTGGAATGATAAAGATGATAAGGGTGGGAT1220.11653230428303976No Hit
TTCGTAGGGGTAGTTGCAGCTGATGCCTGAATGTATCCTAGCTGACAAATTA1210.11557712146104764No Hit
TGAAGTGGGCAAAACGAAGTGAAGTGAAAATTTAATCAACTGTGTAGACTGT1190.1136667558170634No Hit
GCGTAAGGGATGCTAGATCTTGACATGCCCTCAACTCAGTGGTACTTCTTTA1180.11271157299507127No Hit
TCCCATGGGAGATTCTGCTAGCCTGTTCTTTTACCACTGACAGCTAGACACC1180.11271157299507127No Hit
GGTAGTGGGATTTGCTCTCCATTAGCAAAATAATTCATTGTTAGGTAAACTT1180.11271157299507127No Hit
ACAGTGGGCAACATTCAGTATCAGAAGAAAATATAAATTAAAACCACAATGA1180.11271157299507127No Hit
GCGATGGGTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTA1180.11271157299507127No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTT1170.11175639017307912No Hit
GTTAGTGGGTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAG1160.11080120735108699No Hit
GTTTCAGGGAGAAATTAATAGCACTAAATGCCCACATCAAAAAGCTAGAAAG1160.11080120735108699No Hit
ATATCAGGGACATTAAGAAACCGCACGTGATGTCGCTATGCTCTAACGATCA1160.11080120735108699No Hit
CTGCGAGGGATCACACCAGGCTATGGCAATCATGATGCTATGCCATTTCTAA1150.10984602452909487No Hit
ATGCACGCGGATGGCTGCTTCTGTGCTTCATAGCAAAGTTGAATAGGTGTAA1150.10984602452909487No Hit
CGCGTGGGGAATTGTTAGAAAGCAGATACCATCAAATTGGTTCTGGGAAGTG1150.10984602452909487No Hit
TCAAAAGGGCTCACCAAGATGAACTATTTGCCGCCCTGTCTTTTCCTGGGTT1140.10889084170710274No Hit
CAGGTTGGGACTCTCCTGAATACTTATTTGTTGTTTGTCACTTAATTCTCAG1140.10889084170710274No Hit
GCGTAAAGGATTTGGCAAAATTGAAAACTTTTTTCATGATTTATAAAAACAA1140.10889084170710274No Hit
TCTCTCGGGGTAGACATATTTTTGCTCTCCTGATTTGCAGTAAGATTTTAAA1140.10889084170710274No Hit
CTAGACGGGGCAATTAGCCTGTAGATAAGTTTTGTGGAAATTGCCACCTTAA1130.10793565888511061No Hit
ACTTGTGGGTGTTGCTGCTGTCCCCAGTGATGGTGGACTTATCTTTGCCTTA1120.10698047606311847No Hit
CGCTTTGGGGTAGGTCTTCAATAATGTTTGTTTGCTTAATTACTAAATTGAA1120.10698047606311847No Hit
TGTGGCTGGGGATTGTTGGGCTATTTGCATGATTTCCTAAAATATCTGGCAA1120.10698047606311847No Hit
TGGCTATGGGAGAGTTTCTAACCTAATTTGTAATGCTGTAATCACATACTTA1110.10602529324112635No Hit
TCTCGGGGGCATAGGCATTCTCGGGACCGTCCTTATCCCATTTAATTAATTT1110.10602529324112635No Hit
CTTCAGGGGCCCATGTAACTTAGGTAGAAAAAACTGGAGAAACTAAATTGTT1110.10602529324112635No Hit
AGTGCTGGGCCATATCCTAACAATAATGCTCGAAGACCAATGGGACATAAAC1110.10602529324112635No Hit
TTTGCAGGGAATCAAAGGATGGTGCTGGTTAATTTGTCAAACCCATATTTGC1110.10602529324112635No Hit
GGGTCTGGGGTTTGACTGGACAGTAAAGCTTTGGACAACTAAGAATAACAAG1110.10602529324112635No Hit
CGAGTGGGGTATGTGCTCAGTAAATATTTGTTCAATGAATGAATGAATGAAT1100.10507011041913422No Hit
TAGCTGGGATCCCCAGGACGGTGGGCACTGGCCTGGCCAAAACAAGGAACAG1100.10507011041913422No Hit
TGTACGGGGAGCTACATAATTTTTTTTGCTTTTTGCAAATCTTTAATTATTT1100.10507011041913422No Hit
GCGCTAGGGATGTTGGAAATAGAAAACAGATGTTTTAATGGGGTGATACCCA1100.10507011041913422No Hit
AAATTTGGGAGTGGTATATGAATGTGATTTTGATTTCCACTTTCTAATGACT1100.10507011041913422No Hit
AATCCAGGGCCCTGCGGGAGTCAATGATCCAGCTAGCTGAGGGGCTTATCAC1100.10507011041913422No Hit
TTTTCCGGGAGGAAGCACGGTACCTCCTACACTCTCTTCTCCCCACATCAAC1090.1041149275971421No Hit
GCCATGGGGATTGTTTCAAAAGAAATTCTAAATACTTTCAAACTATAATAGA1090.1041149275971421No Hit
TAGCTGGGGGTAGGAACTTTTAGTTCCATTATATGAACACTGGATAACCTAA1090.1041149275971421No Hit
TAGCCTGGGGGGCCGCTTGGAGCTCGTGTGGGGTCTCCGGTCCAGGGCGCGG1090.1041149275971421No Hit
GACCATGGGAAGAAACTGAAAGCTGTATGTCAACTTTATAAACATAATTCAA1090.1041149275971421No Hit
TTTTCTGGGAATTGAAAAAAGAAGGAGTCAAAATATTAAAATGGTACAGATC1090.1041149275971421No Hit
CAATCCGGGAAGTCAAAATATGACTCAAGTGTGAGTGGTCAATCATCTTTTG1080.10315974477514997No Hit
AGGTACGGGGAGGATGAAGAGGGAAATGCCAAACTGCAGCAGCGCCAGCTCA1080.10315974477514997No Hit
GTGACTGGGGAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTT1080.10315974477514997No Hit
CCCCACCATCACTATACAAATAAAAAGAAATCAATAGGCCCAGAAACATAAG1080.10315974477514997No Hit
CAACCCGGGAATTCATTAGATGCCATGTTTTCTTTCTTGGTATCCCCAGGAA1080.10315974477514997No Hit
TACCTTGGGCATTAAAAGCAGAAACTGGTAGCAGAAGAGATTTACAGATTTT1080.10315974477514997No Hit
GGGTTTGGGCAAATACTAATGTGCATATTTTCAAACATACACATATTTTTTT1080.10315974477514997No Hit
GCAGCCAGGAATTCCACACCCTTGTGTTGCGCCCGGAGCCCGCCCTTCGCCT1070.10220456195315783No Hit
GGTACAGGGGGCCCTGATGGGGCCCACCATCCCGGACCCAAGTCTTCTTCCT1070.10220456195315783No Hit
GTTATAGGGAACTTGTTCAGCTCTCCTTGACTCAACTTGGAATATGAAATAC1070.10220456195315783No Hit
TATAAGGGGTGTAGTAATAATAATCAATTTTCAGCTGATAAACAGGGCCAAT1070.10220456195315783No Hit
CTCTATGGGGAAGAGGTAAGAGTAGAAAAATGACCAGAGAAGAGACCATCCC1060.1012493791311657No Hit
CTCTTAGGGATTCTTCAACCACCTCGGTTACTCTGACATAGGAATTTACTTC1060.1012493791311657No Hit
CACAGTGGGGAGCATATTTATGGGCCATTTCTGTTCATCATTCTTTACAGAG1060.1012493791311657No Hit
GTAAGCTGGGGAAGGCGACAGTGCCAGTGGCGGGTTCATTGTGATGGATTTG1050.10029419630917358No Hit
GTGTGTGCACCACCCCCTCACACACACTCACATGCACACACACAGGAAGTAA1050.10029419630917358No Hit
GCAACGGGATCATGATATATAGCATTCTATTGTATAAAGGAACCACAATTTA1050.10029419630917358No Hit
GCAAACGGGAAGTGAATCTCATAGATATCTACAGAACTCTCCAACACGAAAC1050.10029419630917358No Hit
ATCAAAGGGCGATCTGATGCGGGGTGGGTGAGAATGATTACATCAGAGAACT1050.10029419630917358No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)