FastQCFastQC Report
Sat 31 Dec 2022
SRR5849221.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849221.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115925
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT12371.0670692257925383No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC11991.0342894112572785No Hit
TACTCGGGGGCACCGAGCCGGCCGTGCCACCGAGCTGCGGCTCTGGCCCCGG2150.18546474013370712No Hit
CTGTATGGGGGAAAATGGTAGCGATGGCGGCTGGGCCGAGTGGGTGTCTGGT1750.15095967220185466No Hit
ACAAGTGGGCTTTTATATTAGTCCTTTGAAATAGCTATGGACTACCTACTAC1740.15009704550355835No Hit
GAAATAGGGGCCCATCTAATTCAAAAACAGTCCAGTGAAGAAGTAATAATTC1730.14923441880526203No Hit
GAAATAGGGATTGCAGACATGCTAACAATGATTTCCTTATACTTATCCTGGA1710.1475091654086694No Hit
AAAGGAGAGAGACAAGTGCTGCTGTGGGGCGTGAAAGTGCATTGGGAGCCAG1700.1466465387103731No Hit
ATTCACGGGACTACAATGAAATATCAGCTCACATCTGTTAGGATAGCTGTTA1690.14578391201207677No Hit
TCGCGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1670.14405865861548414No Hit
CCACAGGGGACCTTCATTTCTTCTCACGGCACTTCCTCTCCCTGGTATGCCC1640.14147077852059523No Hit
AACCGAGGGCTACGAAGGGGAGCCTAATTGACATGAGAGGCATAGAGACAGT1610.13888289842570628No Hit
GCAAATGGGGGATTTTGAGGTTATTCTGATCCAGGACTACTTGCATGACCAA1610.13888289842570628No Hit
TAATATGGTCTCCCTTTCCTTAGTCAACACTTAAATTTATTTAATGCACCTC1580.13629501833081734No Hit
GTAGTAGGGCAGTTTCGACAAAGAAAAGCTCAGTCGGATGGGCAGAGTCCTT1570.13543239163252102No Hit
GCTCTAGGGAAGAGAAAACTATCAGGTATAAAAAAATTAAAAGTATTCTTTA1560.1345697649342247No Hit
GATCATGGGGTGCTGTTGTGAGTTCTGCTTCAGTTCCTTTGTGTGTGCTGAC1550.1337071382359284No Hit
TACATTGGGCTCACGCAGGGAGTCTAGTGATTTTATGATGGCTCCTTACTAA1550.1337071382359284No Hit
GCATTGGGATCAGCAATAACTACATATTTATAGAAAACACTTTTTCACTTTA1550.1337071382359284No Hit
TGGTCCGGGATTTCCTCAATGTTGGTTTAAGAGTTGTCTAAACCAGTTTCTT1550.1337071382359284No Hit
TAAAATGGGAGAGGTGAAATACAGAAAGCCTTTTTTTCTTGATCTTTTCCCG1550.1337071382359284No Hit
AAACTAGGGGAGAGATATTTGCAGTTGATGTGGAAATTGCCAAACAATCTGT1540.13284451153763208No Hit
ATTCCTGGGTCTTCGAAGGGGAATGCTACAAACATTGCCCCACTGTACTCAC1530.13198188483933576No Hit
TTCATTGGGTTAGGAAAATAGGCACACTTTCAAAGAGAATGTTCTCTTACAC1520.13111925814103947No Hit
AAAGATGGGGGGCATATGTAGGTCTCCTGTCCAACCCGCAAGCTCATGTATC1520.13111925814103947No Hit
CACACTGGGGGCTGGAGGCGCGCGTAGGTTCGAGTAGGGACTGGAATGCGGG1510.13025663144274316No Hit
TAAACGGGACGTATAACAAAACATCAAATAATAAAGCAAAATGTGACAGTCT1500.12939400474444684No Hit
ACTCAAGGGAACTCTTTTTTACTTCAGAAAGAATTTTACAGGTTAAAGGGAA1490.12853137804615053No Hit
GTCTTGGGGGACCTTGTAAGCTTCCTTCCACAGGCTGACCTTGTTTTGTGCA1480.12766875134785421No Hit
TCCTATGGGTCGTGAAGACACTCACTGGCAAGACCATCACCCTTGAGGTCGA1480.12766875134785421No Hit
ACGTCCGGGGAGTCCTTCAGCGGCCGGGTTCGCGCCGCGGTCGCCGGCTGCT1480.12766875134785421No Hit
AATGTCGGGGATGCCTGTGAGGATCATTTGTTAATCAAGGTCAATTTTGGCA1470.1268061246495579No Hit
TGTCGCGGGGTGCTCCTTGAATTCTTTTTTCCTTAAAAAACAATTTTTTAAG1470.1268061246495579No Hit
GCTTTAGGGAAGAACAGGAAGATTACATTTGTTGGCATACGAAACCTTAGTG1470.1268061246495579No Hit
GTACATCGGGGGGGTCACGTGGTACCGGCGCATCACGTGGGCGAGTTAGGTG1460.1259434979512616No Hit
CAGTGGGCTTATCCAAAATCTTTCCAAATCAATTATATTTTGCTGCTTTTCT1460.1259434979512616No Hit
AAGGCAGGGCATTGTAAAGTCAAAAAATCGTAAGTCTTACCATTGTAAGTTG1450.1250808712529653No Hit
AGTAGTGGGAACCATGAAATAAATTAGCCTTTTAGCTAGAAAAAAATGATCA1440.12421824455466897No Hit
CATACAGGGATATGGGAGTTTTATTATGCAGTTGGATCAAAGTATAGATTTT1430.12335561785637265No Hit
CGCGCTGGGAGAGTGGCTGAGGAGCTCTTCAGGCTCATTTTGGAAAATACCT1430.12335561785637265No Hit
TGTTAGGGGTCTTCAAAAAACAGTTTTTCATTTCATTGATCCTTTGTATTTT1430.12335561785637265No Hit
AAACGCGGGAGCGCTAGTGATTCCACAAAGATTCCTGAACCCTGTGGCCTGG1420.12249299115807634No Hit
TTCGCAGGGTCTGAGGCAGGGATATATTTCAAGCTGAATGTTCAGAAAGAAT1420.12249299115807634No Hit
TACATAGGGTGGGAAGAAATCTGGTATCCAAGCTTAAATTTCTTGCTATACA1420.12249299115807634No Hit
TACCCGGGCCTTCATCTTATGAATAAAATGAATTTGCCCACACCTTTTGGAC1410.12163036445978002No Hit
TGACAAGGGTATTTCATGATTGATATGTCACAGAAATCATGGTAGTAAATCA1410.12163036445978002No Hit
GCAAAAGGGATAACTGTATCCTGTGGGTTTTCCCAAGAATCACTCTAAGTAC1410.12163036445978002No Hit
TACCTAGGGGAGATTGTGAAGAGTCACTGAGGAATTTATGTCCATCAAAAGT1400.12076773776148372No Hit
AGGGTAGGGCTGGACATTTCATATGAATGTAAACATACAATAAGTGGTCTTT1400.12076773776148372No Hit
AAGACAGGGGAAGTCCAGAGAAAAATAAATTTTAAAAAAAGGTGGAAAAGTT1390.11990511106318741No Hit
AAATCAGGGTGACAGATTCAGCGAATAAAATTACTGGAAGCCCCATTAAATT1390.11990511106318741No Hit
GCGTTGGGGTATGTCCCTGTGTGCTCTATGTTTAGCTCCCACTTACAAGTAA1380.1190424843648911No Hit
GCACATGGGAAGTAATCTTCTACTATTAACAAGTTCAGTGGTTTTATTTTTA1380.1190424843648911No Hit
CGTAATGGGTCATTCTCTGCAAGTTTGTCCCTAATGCTTAAGAATGAGTTTT1380.1190424843648911No Hit
CTTTGCGGGGACACTGCTGGTGGGGATGTAGACTAGTACAATCATTATGGAA1370.11817985766659478No Hit
TGTAAAGGGTATCATCATGGACTGGCAATCCTAAACAAAATCAGTGATAAAA1370.11817985766659478No Hit
TATGCTGGGGGAGAGCATTGCTTTTCTATCTAGAAGTTCGTTACCTCCTGCA1360.11731723096829846No Hit
GCTCCTGGGATCTTATCTGCTGTCAGGTCAACTGGACACACTTGGAAAATGC1360.11731723096829846No Hit
AGCAGGGGGGTGCGGAGGATGGGAAGGCGACTCTGAAGGGTGGGAAGTGAAT1350.11645460427000215No Hit
CTTTTAGGGGGCGGGCGCCGGAGAGGAAGGAAGGCTGGCAGCCTCGTCACGT1340.11559197757170583No Hit
GCAATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1340.11559197757170583No Hit
TCGTCAGGAAGAAAGGTAGATTTAGGAATAAACCTTTTGAGATAAAAAAAAT1310.1130040974768169No Hit
AGCTTTGGGCTCATTTTCTTGATTATTATTTACAGAAGTTTTAAATTTTGAT1310.1130040974768169No Hit
CCATCAGGGTGATGATACCTTCTTTAAAAGCTTTGACTTACTGAAATAATGC1310.1130040974768169No Hit
AAGCATGGGTGACTGATTTTTGGAAGGTAGATCAAAGGATCTAGGGGTAGAT1310.1130040974768169No Hit
ACGCAGGGGATAAGAGTGGAGGAGGAATGCCTTCTCCTAAGAGTCAGTTGAA1310.1130040974768169No Hit
GCATAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1300.11214147077852059No Hit
TAGTCAGGGTCCCTTACATTCCTTTATATAAGAAAAGCAACATGCAACGCGA1290.11127884408022427No Hit
TCGCAGGGGGCAGCAGTGAGCTATGATTGTGCCACTGTACTCCAGTCTGGGT1290.11127884408022427No Hit
CATGAGGGGAATGATATACGGTATCAGAGACTGGTGGTGGAGCTGCTTATCA1290.11127884408022427No Hit
TGAAATAGGGTCATATTCATTTCTAACTGAGGCTGTGTGGGTGAAACTAAAA1290.11127884408022427No Hit
TGGTATGGGATTGCTAATTAATCATTTCGCCTTATTTAATTTTATGCTTTTG1290.11127884408022427No Hit
TTTCTAGGGAGTGGAATCGCGACTATGGGAGCTCCGGGGGGAAAGATCAACC1280.11041621738192796No Hit
AAAATAGGGACGTTGACAGTGTTGAAGCAGGGTGAATAACTAGGGCATATAT1280.11041621738192796No Hit
ACATGTGGGCAAGATAACGTGACTTCATGAGGGAGAATGTCACTATTAATTT1280.11041621738192796No Hit
GCTGCTGGGCAAATCACAACATGTTTAACTGAAATGGCTGTATTGTAATTAA1280.11041621738192796No Hit
AGGTGGGCCGATCTACAACGGGGAAGGTGAAATTGCCTTGGTATCTAATGAA1280.11041621738192796No Hit
CTGGCTCGGGTGGCGACTTTGGTCAGTCATCTAGTTTTTCCGGGCCTTATGT1280.11041621738192796No Hit
CGAGCTGGGAGACTGGCTAATTAGATGGAAACTTTGATGGTTAGGTTCTTTA1280.11041621738192796No Hit
ATATCTGGGTGTTGAAAATCAGTTTAATGTATTTTGTCTGTTTTGTTTTTTA1280.11041621738192796No Hit
TTCTGCGGGATAATGAAATATCTTCTTTCTGCTAAAGATTACAATTTTTCAT1270.10955359068363167No Hit
GTCATTGGGCGATCTGATGAGGGGCATATTGACTTGTCAGTACCTTTAACTC1270.10955359068363167No Hit
ATCCTTGGGAGGACCAGACCAGAAACTGCTAAAGAACATGGCCTGTTTGACA1270.10955359068363167No Hit
TCATAAGGGAGGCAACCTCCCATTTTAGATGTTTAAAGGACCCTATATGTGG1270.10955359068363167No Hit
TGTTTGCGGGAAGGGAATGCTAGAAAATAGATGAGAAGTACTGAAAGACCTT1270.10955359068363167No Hit
TGCTGGGGACTAGCTTCTTTCTCTATAAGAGTGCCTCTTTCATAAAACCAAA1260.10869096398533536No Hit
GTGTGCGGGTACATATATATACTTTTTTTTTAAGTGAAGGGTCAATACATGG1260.10869096398533536No Hit
TTCTGCGGGATCAAGTAACAGAAGCATTTAAGCTTATCACTTCAGATAAAAA1260.10869096398533536No Hit
CCACATGGGAAATTGATCAGATTGGATTTATCTTGAAAACAGTTCTGCATCT1260.10869096398533536No Hit
GTCGCAGGGATGACTAATTTTAAAGCAAAATGTTGATTGATATACAAATAAA1250.10782833728703904No Hit
TTATTGGGCTTCCTCAAGCTAATGAAGGAAGTAGGACCTCAGCAGGTGCTTT1250.10782833728703904No Hit
CACGCAGGGCAGGCATCAGGAAGTGCATCTAAGAAAGGATTTAAACCTGGTT1250.10782833728703904No Hit
TAAACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1250.10782833728703904No Hit
ACTTGTGGGGTGTTCACTCTTTTATACAAATGAAGAAGTTGCAAAGAATACT1250.10782833728703904No Hit
TGTTTGGGGCTGGCTCTAGTCTTGTCTTCCTTGGCTCTCTACACTGAAACTT1250.10782833728703904No Hit
TGATTAGGGCACGTTTCAGGCCCCAGTTTTAACTCAAGGCCTGGGGCAGGGG1240.10696571058874271No Hit
CTTACCGGTTTTGAAATACTATTGTATTTATACAATGCAAAGGAAAGATCGC1240.10696571058874271No Hit
TGAACTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1240.10696571058874271No Hit
CAAGAGGGGACCTACCTCAAAAAAATTAGAAAGATGTCAATTTAATTATCTA1240.10696571058874271No Hit
CGTTACGGGATCATCAGGCTGATGAGCCATGGTGGAATATTTGCTTCCTTGT1230.1061030838904464No Hit
TCCACAGGGCGATCTAGCCAAGGGATTTTAATTTTGAATGGAATAATTTCAA1230.1061030838904464No Hit
ATGTTAGGGATGAAATTCTGGGTTGAAAATTCTTTTCTTTAAGAATGTTGAA1230.1061030838904464No Hit
GCATTTGGGATTTGCATGTTATAGAAATTAAAATATGTAGGACAAAGATTTA1230.1061030838904464No Hit
ACGTGCGGGAGCAACAAGGAAGAGAGACCATGACTCTATCGATGACACTGTT1230.1061030838904464No Hit
TTCATAGGGCAACTATAGTTTTTCTAAACCTTCATCATTTTGTGATTCTTTG1230.1061030838904464No Hit
GAATCAGGGGTGACATATAAGGTAAAAGTTTCTGGAATCCACAAAATTCTGG1230.1061030838904464No Hit
GTAACAGGGATTCATTTTTGTGTAAGGGAAATGAGATGATGTATCCCAAGGG1230.1061030838904464No Hit
GCAGGAGGGAGAGCTGCAAAATGCTATATTTTAAGTTTAAATTCTATGAGAA1220.10524045719215008No Hit
CCACCAGGGAGGGTTTCACTATGTTGGCCGGGCTGGTCTCCAACTCCTGACC1220.10524045719215008No Hit
AATAGGGGTGGAATCTTTTCTGTACAAGATAGGATTCATTGCAAACAAGCTT1220.10524045719215008No Hit
GCCGTTGGGAGAAAAAACAACATTGAATATTATCAATATTCTCAGAGCTCTT1220.10524045719215008No Hit
CGGCGGGCGCTCTTCCGATCTAAACAAGGGTATCAATTTCCACATGAAAAAG1220.10524045719215008No Hit
TACACAGGGGTCTCAGTCAGACTTAAGAGTTTTCACAACTCATTTCAAGCTG1210.1043778304938538No Hit
AGCCAGGGGTCTTTTTTTATATAATGAACCACTCTTTTTTAACTTTGTAACC1210.1043778304938538No Hit
GCCTTGGGGCATGATTTACGGGAGCAGATGATGAATAGTAGTATTAGTTCAG1210.1043778304938538No Hit
TGAATTGGGACGCTCTTCCGATCTTGCAAAGGGAGTCTTCTGCTTCTACACT1210.1043778304938538No Hit
TTCAATGGGGAAAATTTTCAGATTTTTATTTTAGTATTTTTACCTTCATTAT1210.1043778304938538No Hit
TTGCAGGGAGTCTTATTGATTATAGAGTGAAATGAATCTCACTCAGAGGCAG1200.10351520379555748No Hit
GCGTATGGGATATTGGCAGACCTGAGTCATATTAATGGTAGTTGTTTTTTTC1200.10351520379555748No Hit
CTGGAGGGGTCTTTCAGGATATTTATCCACCTACTGTCTTCTTTGCCTTAAA1200.10351520379555748No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCCTGTCTCTTATAC1190.10265257709726117No Hit
CACAAGGGAAACAAAAAGCTCCTCAACAATACCGCATCCGAGCAAAATTGAG1190.10265257709726117No Hit
GCATTGGGGTATCAAGCCAAGAACTTCTTCATCAGAATTGCTGTTCCTGAAC1190.10265257709726117No Hit
GTAAGGGGGACTGTGGATACTGAATCAGTGTACTATTGGCTGCAGAATTTGT1180.10178995039896485No Hit
TGTTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1180.10178995039896485No Hit
ACGCATGGGAAATGTCTGTGAGATTTTGATTTATATGATTAAAAGAGTGAGT1180.10178995039896485No Hit
TTGCTGGGAAGGAAATGAATGTTCCATTGAACAGATGGAACATGTTCGGGGA1180.10178995039896485No Hit
ATTAGTGGGTGACAGAGTGAGACCACCTCTCAAAAACACAAAGAAATTTAAA1180.10178995039896485No Hit
CTTTAAGCGGGATTTAGTGAAACGACAGAGCAGAGACTATGATGTGGAATGG1180.10178995039896485No Hit
GCATTTGGGAAGACTGTTGCAGCTGAATTAGCCATTTTCAGAGTCTTCAACA1180.10178995039896485No Hit
CAATAAGGGATTGAATTGTTTCATTCTAGCTTGAATATCGAAATGGAATGTG1180.10178995039896485No Hit
GAGAGAGGGATGGGGCAGAAATGAAAATTCTGTTCCTCTTGGTAGGTCCAAA1180.10178995039896485No Hit
CATACAGGGACAGATGAGAATGCTGAGGAAGGCCAGGTTAATTACCTAAAGC1180.10178995039896485No Hit
ATGACAGGGGCCAGATTGCAGCAGAAACTTCAACTCTAAAATTACTTTGCTA1180.10178995039896485No Hit
GATTCAGGGGAGCAAAGCCTTCCTCAGCAGAGATTCCAGCTCTCCTCTGCCT1180.10178995039896485No Hit
GCTGTCGGGAGGATGATCAAACTTGGTCTGGGTATTGATGAAGATGACCCTA1170.10092732370066854No Hit
TTAAATGGGAGGGCCACTGCCATGACCCAGCTGCTCATGAGTGATAAATGTC1170.10092732370066854No Hit
TTTATAGGGATTGAAACAGCGATGATATAAAAAGAAATACTGTCCCTACCAA1170.10092732370066854No Hit
TTGTATGGGACCCAAAATCACTGAAGAAAATCGCTGGCATCAGTGTGCAGCA1170.10092732370066854No Hit
AGTACAGGCATGCTCAGCCTTTTGTGCTTTTTGTTGATAATTTCACCACTTA1160.10006469700237222No Hit
GGCAAGGGGGAAACCGACAGCAAGTAGTTGTAGCTATATTCATATCAGGAAA1160.10006469700237222No Hit
AAAACTGGGCGATCTGCGTAGGGGATCGCCAGAGGTGCTTCACCCTGAGTTT1160.10006469700237222No Hit
TACAACGGGAACTGCAAGAGGCATGCCTTCCTCTTATACTAATCCTCCTCAG1160.10006469700237222No Hit
TATTTAGGGAGCTGTATGATTTGATAACCAATTCCCTCCTCCATTCGTCAGA1160.10006469700237222No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)