FastQCFastQC Report
Sat 31 Dec 2022
SRR5849220.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849220.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115026
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC12421.0797558812790151No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT12341.0728009319632084No Hit
TACTCGGGGGCACCGAGCCGGCCGTGCCACCGAGCTGCGGCTCTGGCCCCGG1860.16170257159250953No Hit
GCAAATGGGGGATTTTGAGGTTATTCTGATCCAGGACTACTTGCATGACCAA1830.15909446559908197No Hit
GCGTATGGGATATTGGCAGACCTGAGTCATATTAATGGTAGTTGTTTTTTTC1810.15735572827013025No Hit
ATTCACGGGACTACAATGAAATATCAGCTCACATCTGTTAGGATAGCTGTTA1790.1556169909411785No Hit
AAAGGAGAGAGACAAGTGCTGCTGTGGGGCGTGAAAGTGCATTGGGAGCCAG1730.15040077895432336No Hit
AAGACAGGGGAAGTCCAGAGAAAAATAAATTTTAAAAAAAGGTGGAAAAGTT1680.14605393563194408No Hit
AACCGAGGGCTACGAAGGGGAGCCTAATTGACATGAGAGGCATAGAGACAGT1650.14344582963851651No Hit
AGTAGTGGGAACCATGAAATAAATTAGCCTTTTAGCTAGAAAAAAATGATCA1630.1417070923095648No Hit
CTGTATGGGGGAAAATGGTAGCGATGGCGGCTGGGCCGAGTGGGTGTCTGGT1620.14083772364508895No Hit
GTACATCGGGGGGGTCACGTGGTACCGGCGCATCACGTGGGCGAGTTAGGTG1600.13909898631613724No Hit
TACCCGGGCCTTCATCTTATGAATAAAATGAATTTGCCCACACCTTTTGGAC1600.13909898631613724No Hit
TTCGCAGGGTCTGAGGCAGGGATATATTTCAAGCTGAATGTTCAGAAAGAAT1590.13822961765166136No Hit
GTCTTGGGGGACCTTGTAAGCTTCCTTCCACAGGCTGACCTTGTTTTGTGCA1580.13736024898718552No Hit
TACCTAGGGGAGATTGTGAAGAGTCACTGAGGAATTTATGTCCATCAAAAGT1580.13736024898718552No Hit
TGGTCCGGGATTTCCTCAATGTTGGTTTAAGAGTTGTCTAAACCAGTTTCTT1580.13736024898718552No Hit
GAAATAGGGGCCCATCTAATTCAAAAACAGTCCAGTGAAGAAGTAATAATTC1580.13736024898718552No Hit
GCGTTGGGGTATGTCCCTGTGTGCTCTATGTTTAGCTCCCACTTACAAGTAA1550.13475214299375793No Hit
GATCATGGGGTGCTGTTGTGAGTTCTGCTTCAGTTCCTTTGTGTGTGCTGAC1540.13388277432928208No Hit
GCCAATGGGATAACCTATTTATTTCAAGTGTTCATATTTGAATTTCTTTGGG1540.13388277432928208No Hit
GCTCTAGGGAAGAGAAAACTATCAGGTATAAAAAAATTAAAAGTATTCTTTA1530.1330134056648062No Hit
GTGTGCGGGTACATATATATACTTTTTTTTTAAGTGAAGGGTCAATACATGG1520.13214403700033034No Hit
TGACAAGGGTATTTCATGATTGATATGTCACAGAAATCATGGTAGTAAATCA1510.1312746683358545No Hit
GCTTTAGGGAAGAACAGGAAGATTACATTTGTTGGCATACGAAACCTTAGTG1510.1312746683358545No Hit
AATGTCGGGGATGCCTGTGAGGATCATTTGTTAATCAAGGTCAATTTTGGCA1500.13040529967137865No Hit
CATGAGGGGAATGATATACGGTATCAGAGACTGGTGGTGGAGCTGCTTATCA1460.12692782501347522No Hit
CTCAGCAAGGGATTTCTCAGGTGTTGAAGTGATAGGTGATTTGATTTTCATT1460.12692782501347522No Hit
AGCAGGGGGGTGCGGAGGATGGGAAGGCGACTCTGAAGGGTGGGAAGTGAAT1460.12692782501347522No Hit
TAAAATGGGAGAGGTGAAATACAGAAAGCCTTTTTTTCTTGATCTTTTCCCG1460.12692782501347522No Hit
TACATAGGGTGGGAAGAAATCTGGTATCCAAGCTTAAATTTCTTGCTATACA1460.12692782501347522No Hit
AGCTTTGGGCTCATTTTCTTGATTATTATTTACAGAAGTTTTAAATTTTGAT1450.12605845634899934No Hit
GAATCAGGGGTGACATATAAGGTAAAAGTTTCTGGAATCCACAAAATTCTGG1450.12605845634899934No Hit
AAGCATGGGTGACTGATTTTTGGAAGGTAGATCAAAGGATCTAGGGGTAGAT1450.12605845634899934No Hit
CACAAGGGAAACAAAAAGCTCCTCAACAATACCGCATCCGAGCAAAATTGAG1440.1251890876845235No Hit
GCAAAAGGGATAACTGTATCCTGTGGGTTTTCCCAAGAATCACTCTAAGTAC1440.1251890876845235No Hit
TAAACGGGACGTATAACAAAACATCAAATAATAAAGCAAAATGTGACAGTCT1440.1251890876845235No Hit
ACAAGTGGGCTTTTATATTAGTCCTTTGAAATAGCTATGGACTACCTACTAC1440.1251890876845235No Hit
AAACTAGGGGAGAGATATTTGCAGTTGATGTGGAAATTGCCAAACAATCTGT1440.1251890876845235No Hit
TGTCGCGGGGTGCTCCTTGAATTCTTTTTTCCTTAAAAAACAATTTTTTAAG1440.1251890876845235No Hit
TAAACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1440.1251890876845235No Hit
GTAGTAGGGCAGTTTCGACAAAGAAAAGCTCAGTCGGATGGGCAGAGTCCTT1440.1251890876845235No Hit
TGTAGGGGGGAGACTGCGGGACGGACGGTGGACGCTGGGACGCGTTTGTAGC1430.12431971902004764No Hit
GTTTCAGGGAACACCATGAAACTATTCTTTCCATCTCTCTCTTGAGTGCTTC1420.12345035035557178No Hit
TTAAGAGGGGAGTAAATAAAGCCAGTTTATGACTTCCCACAAATACAATGGG1420.12345035035557178No Hit
ACTCAAGGGAACTCTTTTTTACTTCAGAAAGAATTTTACAGGTTAAAGGGAA1420.12345035035557178No Hit
ATATCTGGGTGTTGAAAATCAGTTTAATGTATTTTGTCTGTTTTGTTTTTTA1420.12345035035557178No Hit
TAATATGGTCTCCCTTTCCTTAGTCAACACTTAAATTTATTTAATGCACCTC1410.12258098169109592No Hit
CACGCAGGGCAGGCATCAGGAAGTGCATCTAAGAAAGGATTTAAACCTGGTT1400.12171161302662006No Hit
TGAAATAGGGTCATATTCATTTCTAACTGAGGCTGTGTGGGTGAAACTAAAA1400.12171161302662006No Hit
TATGCTGGGGGAGAGCATTGCTTTTCTATCTAGAAGTTCGTTACCTCCTGCA1400.12171161302662006No Hit
GAAATAGGGATTGCAGACATGCTAACAATGATTTCCTTATACTTATCCTGGA1390.1208422443621442No Hit
TCCACAGGGCGATCTAGCCAAGGGATTTTAATTTTGAATGGAATAATTTCAA1390.1208422443621442No Hit
ATCCTTGGGAGGACCAGACCAGAAACTGCTAAAGAACATGGCCTGTTTGACA1390.1208422443621442No Hit
AAGGCAGGGCATTGTAAAGTCAAAAAATCGTAAGTCTTACCATTGTAAGTTG1370.1191035070331925No Hit
ATTCCTGGGTCTTCGAAGGGGAATGCTACAAACATTGCCCCACTGTACTCAC1370.1191035070331925No Hit
GCAGGAGGGAGAGCTGCAAAATGCTATATTTTAAGTTTAAATTCTATGAGAA1360.11823413836871664No Hit
TGATTAGGGCACGTTTCAGGCCCCAGTTTTAACTCAAGGCCTGGGGCAGGGG1360.11823413836871664No Hit
AGTCTCGGGGCTGGGAAACAGATTTGTGAATTGGATAGGAAGATCCATTTGA1360.11823413836871664No Hit
TCGCAGGGGGCAGCAGTGAGCTATGATTGTGCCACTGTACTCCAGTCTGGGT1360.11823413836871664No Hit
TGAATTGGGACGCTCTTCCGATCTTGCAAAGGGAGTCTTCTGCTTCTACACT1360.11823413836871664No Hit
GCCTTGGGGCATGATTTACGGGAGCAGATGATGAATAGTAGTATTAGTTCAG1350.11736476970424078No Hit
CACACTGGGGGCTGGAGGCGCGCGTAGGTTCGAGTAGGGACTGGAATGCGGG1350.11736476970424078No Hit
GAGAGAGGGATGGGGCAGAAATGAAAATTCTGTTCCTCTTGGTAGGTCCAAA1350.11736476970424078No Hit
ATACCTGGGAAATGATTGGTATCAATGTATATAGAGTAAAATTATTGCAAAA1340.11649540103976493No Hit
TCATAAGGGAGGCAACCTCCCATTTTAGATGTTTAAAGGACCCTATATGTGG1340.11649540103976493No Hit
ACGCAGGGGATAAGAGTGGAGGAGGAATGCCTTCTCCTAAGAGTCAGTTGAA1340.11649540103976493No Hit
GCACATGGGAAGTAATCTTCTACTATTAACAAGTTCAGTGGTTTTATTTTTA1330.11562603237528907No Hit
TTTCTAGGGAGTGGAATCGCGACTATGGGAGCTCCGGGGGGAAAGATCAACC1330.11562603237528907No Hit
CCACATGGGAAATTGATCAGATTGGATTTATCTTGAAAACAGTTCTGCATCT1330.11562603237528907No Hit
GCTGTCGGGAGGATGATCAAACTTGGTCTGGGTATTGATGAAGATGACCCTA1320.11475666371081321No Hit
AAAGATGGGGGGCATATGTAGGTCTCCTGTCCAACCCGCAAGCTCATGTATC1320.11475666371081321No Hit
TTATTGGGCTTCCTCAAGCTAATGAAGGAAGTAGGACCTCAGCAGGTGCTTT1320.11475666371081321No Hit
CTTTATGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT1320.11475666371081321No Hit
TACATTGGGCTCACGCAGGGAGTCTAGTGATTTTATGATGGCTCCTTACTAA1310.11388729504633735No Hit
ACGTCCGGGGAGTCCTTCAGCGGCCGGGTTCGCGCCGCGGTCGCCGGCTGCT1310.11388729504633735No Hit
CCACAGGGGACCTTCATTTCTTCTCACGGCACTTCCTCTCCCTGGTATGCCC1300.1130179263818615No Hit
TTCCTTGGGCTTGACGCTCTTCTTTTCATTATTTCTAATGCTACAAGAATGC1300.1130179263818615No Hit
AGCACAGGGAAGACTTGAAGGAAGCAAAGACGCCCTGCATGGTTACAATGGC1300.1130179263818615No Hit
AAAATAGGGACGTTGACAGTGTTGAAGCAGGGTGAATAACTAGGGCATATAT1290.11214855771738565No Hit
GCAATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1290.11214855771738565No Hit
TCGCGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1290.11214855771738565No Hit
CATACAGGGATATGGGAGTTTTATTATGCAGTTGGATCAAAGTATAGATTTT1280.11127918905290979No Hit
GCATTTGGGATTTGCATGTTATAGAAATTAAAATATGTAGGACAAAGATTTA1280.11127918905290979No Hit
CAGTGGGCTTATCCAAAATCTTTCCAAATCAATTATATTTTGCTGCTTTTCT1280.11127918905290979No Hit
TTGCGAGGGTGATGCGGGCTGTTTGCAAAATAATATTCTGTTTTAAGAATCC1280.11127918905290979No Hit
TCGTCAGGAAGAAAGGTAGATTTAGGAATAAACCTTTTGAGATAAAAAAAAT1270.11040982038843393No Hit
TGCCGTGGGGAACTAAGTGGATCAAAATGATTTGCTTATTTTTATTTTTTTT1270.11040982038843393No Hit
GCATTGGGATCAGCAATAACTACATATTTATAGAAAACACTTTTTCACTTTA1270.11040982038843393No Hit
TTATTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1270.11040982038843393No Hit
TGTTTGGGGCTGGCTCTAGTCTTGTCTTCCTTGGCTCTCTACACTGAAACTT1270.11040982038843393No Hit
ATTCTAGGGGAGGTACCCGCAACCACACCCAGCTTATTTTGTGTTTTTGGCG1260.10954045172395807No Hit
AATAGGGGTGGAATCTTTTCTGTACAAGATAGGATTCATTGCAAACAAGCTT1260.10954045172395807No Hit
GGCAAGGGGGAAACCGACAGCAAGTAGTTGTAGCTATATTCATATCAGGAAA1260.10954045172395807No Hit
CTTTTAGGGGGCGGGCGCCGGAGAGGAAGGAAGGCTGGCAGCCTCGTCACGT1260.10954045172395807No Hit
CACTTTGGGACCTTCATATTCCACGCATGAACTCTGTGAGCGATTTGCCCGA1250.1086710830594822No Hit
ATCAGCGGGTGAAAAGGCATTCAAACATCATTTATTATTAATATTCTATTTT1240.10780171439500634No Hit
TCTAGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1240.10780171439500634No Hit
GTAACAGGGATTCATTTTTGTGTAAGGGAAATGAGATGATGTATCCCAAGGG1240.10780171439500634No Hit
TTCTGCGGGATAATGAAATATCTTCTTTCTGCTAAAGATTACAATTTTTCAT1230.10693234573053048No Hit
TTAAATGGGAGGGCCACTGCCATGACCCAGCTGCTCATGAGTGATAAATGTC1230.10693234573053048No Hit
TCTCTAGGGGCATTGTGTAGGTTGCCCGTTCACTCTGATGATAGTTTCTTTT1230.10693234573053048No Hit
TGTGCTGGGGTGTAGTGGGTTTTTAGTAGTGCTGAAGACAAGTTTTCTAAAG1230.10693234573053048No Hit
CTGGCTCGGGTGGCGACTTTGGTCAGTCATCTAGTTTTTCCGGGCCTTATGT1230.10693234573053048No Hit
TCCTATGGGTCGTGAAGACACTCACTGGCAAGACCATCACCCTTGAGGTCGA1230.10693234573053048No Hit
TTCATTGGGTTAGGAAAATAGGCACACTTTCAAAGAGAATGTTCTCTTACAC1220.10606297706605462No Hit
GGTCTGGGGCAAGATACGTCCCATTGAGAATCATGAAAATATTATGACTAAC1220.10606297706605462No Hit
ATTCCTGGGTTGGGACCAAGAATTCCTGAAAGTTGACCAAGGAACACTTTTT1220.10606297706605462No Hit
AAAACTGGGCGATCTGCGTAGGGGATCGCCAGAGGTGCTTCACCCTGAGTTT1220.10606297706605462No Hit
ATGAAAGGGACATAAAGGTAGATGCCTTCTAATGGTTTTCTTCCCACAGGGA1220.10606297706605462No Hit
CCATCAGGGTGATGATACCTTCTTTAAAAGCTTTGACTTACTGAAATAATGC1220.10606297706605462No Hit
CATACAGGGACAGATGAGAATGCTGAGGAAGGCCAGGTTAATTACCTAAAGC1220.10606297706605462No Hit
CAAAGAGGGAGAATATATATTATTTTGTCAAAGTGTCAAAGGTATCCTGTAA1220.10606297706605462No Hit
TTGTATGGGACCCAAAATCACTGAAGAAAATCGCTGGCATCAGTGTGCAGCA1220.10606297706605462No Hit
TAGTCAGGGTCCCTTACATTCCTTTATATAAGAAAAGCAACATGCAACGCGA1210.10519360840157876No Hit
AACTGGGGGAAGAGGTGATAGTTCTGGAAGCAGCAAAAGCATAGGCAGGCTT1210.10519360840157876No Hit
GCGTACGGGAAGGAAATCTGTATTGGCAACTATTATAATTCAATAATTCTTT1200.1043242397371029No Hit
TGAACTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1200.1043242397371029No Hit
CGTAATGGGTCATTCTCTGCAAGTTTGTCCCTAATGCTTAAGAATGAGTTTT1200.1043242397371029No Hit
CTCGCCTCGGGTATCAAAGAGATACTTGCACTTGCATATTTATCACAGCACT1200.1043242397371029No Hit
AATGCTGGGATTACAGGCATGAGCCACCATGCCTGGTTAGCAATATGTTAAG1200.1043242397371029No Hit
ACGCATGGGAAATGTCTGTGAGATTTTGATTTATATGATTAAAAGAGTGAGT1190.10345487107262705No Hit
TACTGCGGGCGATCTATCTATGGGAGAGATTAACCGTTATGTTGAAGTAAGA1190.10345487107262705No Hit
TTCTGGGGGAGTTACAATCTCATGTTTGTGTTTTCTTAGTACTGTCTATTTA1190.10345487107262705No Hit
GCGGCTGGGAAGAAAAGAGCTATAAGGGAACATGTATCCTTAGAGACCTTAC1180.1025855024081512No Hit
AGAATGGGGACCTACAGCAGTAAAAACTAAAATTGTCTCTAAACATTGACAA1180.1025855024081512No Hit
TATTTAGGGATTAACAGCTTATAAAAAATTTGTCTAAATTTAATAATTAGTA1180.1025855024081512No Hit
TGACAGGAGTGAATCACTGCACCTGGCATATGTATTTAAATACTTTTAAAAT1180.1025855024081512No Hit
TACAAGGGGGCCCCTCTCTCGATGTGCACTTTCCAGTGTGGATATATATAGG1180.1025855024081512No Hit
CATACGGGGAGAGTGCCCACCGCATTAGGGATGTTGGGGTGAGTCAGTGTGA1170.10171613374367534No Hit
TGCTAAGGGAACTCAGGCTTCATATATTCACAGACAAATGAAACAATATAAT1170.10171613374367534No Hit
TAGCTGGGGAGAGAATTTTGATAAATCCTGACATTGACCTAATTGACATAGG1170.10171613374367534No Hit
TATGCCGGGCTGGTCTTGAACTCCTGAGCTCAGGTTATCCGCCCGCCTCGGC1170.10171613374367534No Hit
AGGTGGGCCGATCTACAACGGGGAAGGTGAAATTGCCTTGGTATCTAATGAA1170.10171613374367534No Hit
GTAAATGGGGTTTAAGCTGCCAGTTAATTTTAAGTTCTGATGTTTTCTCATT1170.10171613374367534No Hit
ATTACTGGGAGCTTCTATCCTGTGTCCTAGTCGGGGAGACAGAGTGCCAGCC1170.10171613374367534No Hit
ATGACAGGGGCCAGATTGCAGCAGAAACTTCAACTCTAAAATTACTTTGCTA1170.10171613374367534No Hit
GTAGTAAGGGACTTTAGTTGATGAAAAAAAAGCCAAGAAGTGGCCTGGATTT1160.10084676507919949No Hit
TGTTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1160.10084676507919949No Hit
TTGATAGGGCAAGGACGCCATCAAGAAGAAGCTGACAGGGATCAAGCATGAA1160.10084676507919949No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)