FastQCFastQC Report
Sat 31 Dec 2022
SRR5849219.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849219.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116891
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT12131.0377189005141543No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC11971.0240309348025085No Hit
AACCGAGGGCTACGAAGGGGAGCCTAATTGACATGAGAGGCATAGAGACAGT2040.17452156282348513No Hit
GCAAATGGGGGATTTTGAGGTTATTCTGATCCAGGACTACTTGCATGACCAA1830.1565561078269499No Hit
AAAGGAGAGAGACAAGTGCTGCTGTGGGGCGTGAAAGTGCATTGGGAGCCAG1740.14885662711414907No Hit
TACTCGGGGGCACCGAGCCGGCCGTGCCACCGAGCTGCGGCTCTGGCCCCGG1700.1454346356862376No Hit
ACAAGTGGGCTTTTATATTAGTCCTTTGAAATAGCTATGGACTACCTACTAC1680.14372363997228188No Hit
GAAATAGGGGCCCATCTAATTCAAAAACAGTCCAGTGAAGAAGTAATAATTC1680.14372363997228188No Hit
ATTCACGGGACTACAATGAAATATCAGCTCACATCTGTTAGGATAGCTGTTA1670.142868142115304No Hit
TGAAATAGGGTCATATTCATTTCTAACTGAGGCTGTGTGGGTGAAACTAAAA1660.14201264425832613No Hit
AAGACAGGGGAAGTCCAGAGAAAAATAAATTTTAAAAAAAGGTGGAAAAGTT1650.14115714640134827No Hit
TTCGCAGGGTCTGAGGCAGGGATATATTTCAAGCTGAATGTTCAGAAAGAAT1630.13944615068739252No Hit
ATCAGCGGGTGAAAAGGCATTCAAACATCATTTATTATTAATATTCTATTTT1620.13859065283041466No Hit
GCTCCTGGGATCTTATCTGCTGTCAGGTCAACTGGACACACTTGGAAAATGC1610.1377351549734368No Hit
TGTTTGCGGGAAGGGAATGCTAGAAAATAGATGAGAAGTACTGAAAGACCTT1600.1368796571164589No Hit
CCACATGGGAAATTGATCAGATTGGATTTATCTTGAAAACAGTTCTGCATCT1580.1351686614025032No Hit
AGTAGTGGGAACCATGAAATAAATTAGCCTTTTAGCTAGAAAAAAATGATCA1570.1343131635455253No Hit
TAATATGGTCTCCCTTTCCTTAGTCAACACTTAAATTTATTTAATGCACCTC1560.13345766568854744No Hit
TATGCTGGGGGAGAGCATTGCTTTTCTATCTAGAAGTTCGTTACCTCCTGCA1560.13345766568854744No Hit
CATACAGGGATATGGGAGTTTTATTATGCAGTTGGATCAAAGTATAGATTTT1540.13174666997459172No Hit
TAAAATGGGAGAGGTGAAATACAGAAAGCCTTTTTTTCTTGATCTTTTCCCG1540.13174666997459172No Hit
AAAGATGGGGGGCATATGTAGGTCTCCTGTCCAACCCGCAAGCTCATGTATC1510.12918017640365811No Hit
GCAAAAGGGATAACTGTATCCTGTGGGTTTTCCCAAGAATCACTCTAAGTAC1510.12918017640365811No Hit
GCTGCTGGGCAAATCACAACATGTTTAACTGAAATGGCTGTATTGTAATTAA1510.12918017640365811No Hit
TGACAAGGGTATTTCATGATTGATATGTCACAGAAATCATGGTAGTAAATCA1500.12832467854668023No Hit
TTGTATGGGACCCAAAATCACTGAAGAAAATCGCTGGCATCAGTGTGCAGCA1490.12746918068970237No Hit
GCACATGGGAAGTAATCTTCTACTATTAACAAGTTCAGTGGTTTTATTTTTA1480.1266136828327245No Hit
ATATCTGGGTGTTGAAAATCAGTTTAATGTATTTTGTCTGTTTTGTTTTTTA1480.1266136828327245No Hit
GAAATAGGGATTGCAGACATGCTAACAATGATTTCCTTATACTTATCCTGGA1450.1240471892617909No Hit
CGTTACGGGATCATCAGGCTGATGAGCCATGGTGGAATATTTGCTTCCTTGT1450.1240471892617909No Hit
TGGTCCGGGATTTCCTCAATGTTGGTTTAAGAGTTGTCTAAACCAGTTTCTT1450.1240471892617909No Hit
TGTTTGGGGCTGGCTCTAGTCTTGTCTTCCTTGGCTCTCTACACTGAAACTT1450.1240471892617909No Hit
AATGTCGGGGATGCCTGTGAGGATCATTTGTTAATCAAGGTCAATTTTGGCA1440.12319169140481304No Hit
GCGCACCGGGAGTATACAAGAATAGTCTTTGAAAATCAAAAAGCTCAATCAG1440.12319169140481304No Hit
GCCAATGGGATAACCTATTTATTTCAAGTGTTCATATTTGAATTTCTTTGGG1430.12233619354783515No Hit
TACCTAGGGGAGATTGTGAAGAGTCACTGAGGAATTTATGTCCATCAAAAGT1420.12148069569085729No Hit
AAAATAGGGACGTTGACAGTGTTGAAGCAGGGTGAATAACTAGGGCATATAT1420.12148069569085729No Hit
CTTGCTGGGAGCTAAGATGTGTTTAGTTTTTAAAATGTGTTAATCATTGTGA1420.12148069569085729No Hit
CGAGCTGGGAGACTGGCTAATTAGATGGAAACTTTGATGGTTAGGTTCTTTA1420.12148069569085729No Hit
GTACATCGGGGGGGTCACGTGGTACCGGCGCATCACGTGGGCGAGTTAGGTG1400.11976969997690157No Hit
GCATTGGGATCAGCAATAACTACATATTTATAGAAAACACTTTTTCACTTTA1400.11976969997690157No Hit
TAAACGGGACGTATAACAAAACATCAAATAATAAAGCAAAATGTGACAGTCT1400.11976969997690157No Hit
TAAACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1400.11976969997690157No Hit
GCTCTAGGGAAGAGAAAACTATCAGGTATAAAAAAATTAAAAGTATTCTTTA1400.11976969997690157No Hit
TGCTTCGGGAAGAACCATATAACTAGTTTTAGCCTCAGTATTAACCTTGTAA1400.11976969997690157No Hit
TACACAGGGGTCTCAGTCAGACTTAAGAGTTTTCACAACTCATTTCAAGCTG1390.11891420211992368No Hit
AAACTAGGGGAGAGATATTTGCAGTTGATGTGGAAATTGCCAAACAATCTGT1390.11891420211992368No Hit
CCACAGGGGACCTTCATTTCTTCTCACGGCACTTCCTCTCCCTGGTATGCCC1380.11805870426294582No Hit
ATACCTGGGAAATGATTGGTATCAATGTATATAGAGTAAAATTATTGCAAAA1380.11805870426294582No Hit
TGTCGCGGGGTGCTCCTTGAATTCTTTTTTCCTTAAAAAACAATTTTTTAAG1380.11805870426294582No Hit
ATTCCTGGGTCTTCGAAGGGGAATGCTACAAACATTGCCCCACTGTACTCAC1380.11805870426294582No Hit
ACGCAGGGGATAAGAGTGGAGGAGGAATGCCTTCTCCTAAGAGTCAGTTGAA1380.11805870426294582No Hit
TACCCGGGCCTTCATCTTATGAATAAAATGAATTTGCCCACACCTTTTGGAC1370.11720320640596796No Hit
CTTTGCGGGGACACTGCTGGTGGGGATGTAGACTAGTACAATCATTATGGAA1370.11720320640596796No Hit
TCGCAGGGGGCAGCAGTGAGCTATGATTGTGCCACTGTACTCCAGTCTGGGT1370.11720320640596796No Hit
CTTTATGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT1360.11634770854899007No Hit
TACATTGGGCTCACGCAGGGAGTCTAGTGATTTTATGATGGCTCCTTACTAA1350.11549221069201221No Hit
GTGTGCGGGTACATATATATACTTTTTTTTTAAGTGAAGGGTCAATACATGG1350.11549221069201221No Hit
TGCTAAGGGAACTCAGGCTTCATATATTCACAGACAAATGAAACAATATAAT1340.11463671283503435No Hit
GCGTATGGGATATTGGCAGACCTGAGTCATATTAATGGTAGTTGTTTTTTTC1340.11463671283503435No Hit
AAGGCAGGGCATTGTAAAGTCAAAAAATCGTAAGTCTTACCATTGTAAGTTG1340.11463671283503435No Hit
AGCAGGGGGGTGCGGAGGATGGGAAGGCGACTCTGAAGGGTGGGAAGTGAAT1340.11463671283503435No Hit
CATACAGGGACAGATGAGAATGCTGAGGAAGGCCAGGTTAATTACCTAAAGC1340.11463671283503435No Hit
TACAACGGGAACTGCAAGAGGCATGCCTTCCTCTTATACTAATCCTCCTCAG1340.11463671283503435No Hit
TTCATTGGGTTAGGAAAATAGGCACACTTTCAAAGAGAATGTTCTCTTACAC1330.11378121497805649No Hit
GATCATGGGGTGCTGTTGTGAGTTCTGCTTCAGTTCCTTTGTGTGTGCTGAC1330.11378121497805649No Hit
GCATTTGGGGACATTTCAGACAGAGAATTTGAAGACCAGTGTTGAAACGCAA1330.11378121497805649No Hit
CACACTGGGGGCTGGAGGCGCGCGTAGGTTCGAGTAGGGACTGGAATGCGGG1330.11378121497805649No Hit
TGTGCTGGGGTGTAGTGGGTTTTTAGTAGTGCTGAAGACAAGTTTTCTAAAG1330.11378121497805649No Hit
GCGTTGGGGTATGTCCCTGTGTGCTCTATGTTTAGCTCCCACTTACAAGTAA1320.1129257171210786No Hit
TTGATAGGGCAAGGACGCCATCAAGAAGAAGCTGACAGGGATCAAGCATGAA1320.1129257171210786No Hit
TTCTGCGGGATAATGAAATATCTTCTTTCTGCTAAAGATTACAATTTTTCAT1310.11207021926410074No Hit
ATTCCTGGGTTGGGACCAAGAATTCCTGAAAGTTGACCAAGGAACACTTTTT1310.11207021926410074No Hit
TTAAGAGGGGAGTAAATAAAGCCAGTTTATGACTTCCCACAAATACAATGGG1310.11207021926410074No Hit
TTCCTTGGGCTTGACGCTCTTCTTTTCATTATTTCTAATGCTACAAGAATGC1310.11207021926410074No Hit
AAATCCGGGACAAATTCTGTACTCAGCATATACAGTATAGTTTATCTGCTCT1310.11207021926410074No Hit
CTGGCTCGGGTGGCGACTTTGGTCAGTCATCTAGTTTTTCCGGGCCTTATGT1310.11207021926410074No Hit
CAATAAGGGATTGAATTGTTTCATTCTAGCTTGAATATCGAAATGGAATGTG1300.11121472140712288No Hit
CTGTATGGGGGAAAATGGTAGCGATGGCGGCTGGGCCGAGTGGGTGTCTGGT1300.11121472140712288No Hit
TTAAATGGGAGGGCCACTGCCATGACCCAGCTGCTCATGAGTGATAAATGTC1290.11035922355014499No Hit
TTATTGGGCTTCCTCAAGCTAATGAAGGAAGTAGGACCTCAGCAGGTGCTTT1290.11035922355014499No Hit
CACGCAGGGCAGGCATCAGGAAGTGCATCTAAGAAAGGATTTAAACCTGGTT1290.11035922355014499No Hit
TTCTGGGGGAGTTACAATCTCATGTTTGTGTTTTCTTAGTACTGTCTATTTA1290.11035922355014499No Hit
GTCATTGGGCGATCTGATGAGGGGCATATTGACTTGTCAGTACCTTTAACTC1290.11035922355014499No Hit
CGTAATGGGTCATTCTCTGCAAGTTTGTCCCTAATGCTTAAGAATGAGTTTT1290.11035922355014499No Hit
GTACAGGGAACGTTTAATTCAATAAATTTGGAAATATATGTAAAATGTGTAC1290.11035922355014499No Hit
GCAATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1290.11035922355014499No Hit
AAAACTGGGCGATCTGCGTAGGGGATCGCCAGAGGTGCTTCACCCTGAGTTT1280.10950372569316713No Hit
TCGCGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1280.10950372569316713No Hit
GCTGTCGGGAGGATGATCAAACTTGGTCTGGGTATTGATGAAGATGACCCTA1270.10864822783618927No Hit
CGTGCAAGGGATGTTTATAACCTTTACTAAGAGATTATTATTTTGCTCTTCA1270.10864822783618927No Hit
CAAACAGGGGCCTGCTTCCAATCTGAAAGACTTCCAGAAGTCTTCCCACCTT1270.10864822783618927No Hit
CCAGCAGGGCACAGTTACTGGATTCAGTGTGGATCAGTTTTGTTAATGATGG1270.10864822783618927No Hit
GCGGCTGGGAAGAAAAGAGCTATAAGGGAACATGTATCCTTAGAGACCTTAC1260.10779272997921141No Hit
TAGTCAGGGTCCCTTACATTCCTTTATATAAGAAAAGCAACATGCAACGCGA1260.10779272997921141No Hit
CTCAGCAAGGGATTTCTCAGGTGTTGAAGTGATAGGTGATTTGATTTTCATT1250.10693723212223352No Hit
CTCGCCTCGGGTATCAAAGAGATACTTGCACTTGCATATTTATCACAGCACT1250.10693723212223352No Hit
ATTTTAGGGGATCCTTGCTTTTTTTAGAGACCTAAAAGGAAAAATTACTCTT1250.10693723212223352No Hit
CATACGGGGAGAGTGCCCACCGCATTAGGGATGTTGGGGTGAGTCAGTGTGA1240.10608173426525566No Hit
AGCTTTGGGCTCATTTTCTTGATTATTATTTACAGAAGTTTTAAATTTTGAT1240.10608173426525566No Hit
TACATAGGGTGGGAAGAAATCTGGTATCCAAGCTTAAATTTCTTGCTATACA1240.10608173426525566No Hit
GCACTTGGGTTGGATTGCTTGCTTCCCTTTTTCCACCTGGGACATTGTAATC1230.1052262364082778No Hit
GTGTTTGGGATATTTACTCAAAACATATGCCAAATTTAGTGTTGAAAGGCTC1230.1052262364082778No Hit
GAGAGAGGGATGGGGCAGAAATGAAAATTCTGTTCCTCTTGGTAGGTCCAAA1230.1052262364082778No Hit
GTAAATGGGGTTTAAGCTGCCAGTTAATTTTAAGTTCTGATGTTTTCTCATT1230.1052262364082778No Hit
AATGCTGGGATTACAGGCATGAGCCACCATGCCTGGTTAGCAATATGTTAAG1230.1052262364082778No Hit
TGCCGGGGGAAAACATAATGGGCAGATTTTCTGTCTTCAAAGTTTCCGGCTA1220.10437073855129991No Hit
AAGCATGGGTGACTGATTTTTGGAAGGTAGATCAAAGGATCTAGGGGTAGAT1220.10437073855129991No Hit
ATGACAGGGGCCAGATTGCAGCAGAAACTTCAACTCTAAAATTACTTTGCTA1220.10437073855129991No Hit
TGTTAGGGGTCTTCAAAAAACAGTTTTTCATTTCATTGATCCTTTGTATTTT1210.10351524069432205No Hit
CAGATAGGGCGATCTACAGAAGGGCCATAAAGCAGACTTTAAATATCCACAT1210.10351524069432205No Hit
AGGTGGGCCGATCTACAACGGGGAAGGTGAAATTGCCTTGGTATCTAATGAA1210.10351524069432205No Hit
ACGGCGGGGTAATCTAGGGACTTGGAGTAGCAGTTTTCTAGCTGCTGCCAGA1210.10351524069432205No Hit
GCCGTTGGGAGAAAAAACAACATTGAATATTATCAATATTCTCAGAGCTCTT1210.10351524069432205No Hit
TCCTATGGGTCGTGAAGACACTCACTGGCAAGACCATCACCCTTGAGGTCGA1210.10351524069432205No Hit
GATTCAGGGGAGCAAAGCCTTCCTCAGCAGAGATTCCAGCTCTCCTCTGCCT1210.10351524069432205No Hit
TAGCTAGGGACACTAATTACAAAATACAAGTTCTGGAAAAAATATTTTTCTT1200.10265974283734419No Hit
GGTCTGGGGCAAGATACGTCCCATTGAGAATCATGAAAATATTATGACTAAC1200.10265974283734419No Hit
TCAGCGGGGAACCCCAAGAAAGTGAAAACTAATATAAAATTAGAAAGACCTA1200.10265974283734419No Hit
GCATTTGGGATTTGCATGTTATAGAAATTAAAATATGTAGGACAAAGATTTA1200.10265974283734419No Hit
ATGAAAGGGCAAGTTAAGCTGACTTTTTTGGTGGGGAACATCATCTTCCTCT1200.10265974283734419No Hit
ACTCAAGGGAACTCTTTTTTACTTCAGAAAGAATTTTACAGGTTAAAGGGAA1200.10265974283734419No Hit
TAAGCGCGGGATTCTTGGGCAGAACTCTGGTCTAGAGAGAACTTTCCTTATT1200.10265974283734419No Hit
AATAGGGGTGGAATCTTTTCTGTACAAGATAGGATTCATTGCAAACAAGCTT1190.10180424498036633No Hit
GTTTCAGGGAACACCATGAAACTATTCTTTCCATCTCTCTCTTGAGTGCTTC1190.10180424498036633No Hit
GAATCAGGGGTGACATATAAGGTAAAAGTTTCTGGAATCCACAAAATTCTGG1190.10180424498036633No Hit
ACTTGTGGGGTGTTCACTCTTTTATACAAATGAAGAAGTTGCAAAGAATACT1190.10180424498036633No Hit
GACAACGGGATGTTGAAAAGGCAAGTGCCCACCCTCTATTCTTCATCTCACC1180.10094874712338844No Hit
GAAAAAGGGGCTTCCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAG1180.10094874712338844No Hit
CATGAGGGGAATGATATACGGTATCAGAGACTGGTGGTGGAGCTGCTTATCA1180.10094874712338844No Hit
TTCAATGGGGAAAATTTTCAGATTTTTATTTTAGTATTTTTACCTTCATTAT1180.10094874712338844No Hit
TCTAATGGGACAGAACTAACATATCATTTGGTAACTGTAAAACCTATTTGCA1180.10094874712338844No Hit
TTGTATGGGGGCCCTGGGCAGTGGGCTGGTCAGCCGGGTGTTCCCAGACAAA1180.10094874712338844No Hit
TTCATAGGGCAACTATAGTTTTTCTAAACCTTCATCATTTTGTGATTCTTTG1180.10094874712338844No Hit
GTAGTAGGGCAGTTTCGACAAAGAAAAGCTCAGTCGGATGGGCAGAGTCCTT1180.10094874712338844No Hit
AAGTAAGGGGTTTAAAACACATATGAAGTTCAAATTTCAGGAACCAAGGACT1180.10094874712338844No Hit
ATTCTAGGGGAGGTACCCGCAACCACACCCAGCTTATTTTGTGTTTTTGGCG1170.10009324926641058No Hit
ACTGCTGGGATTGCAGACGTTTGCCACTGCACCTGGTGGGTGAATTTATTGA1170.10009324926641058No Hit
TGACAGGAGTGAATCACTGCACCTGGCATATGTATTTAAATACTTTTAAAAT1170.10009324926641058No Hit
GTCATGGGGTAAACATATCTTGTTAAGGAAATTACACTTTTAATTTTTCATG1170.10009324926641058No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)