FastQCFastQC Report
Sat 31 Dec 2022
SRR5849218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214786
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC19890.926038009926159No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT18410.8571322153212966No Hit
GCACATGGGGACTTTGGATTTGTTTTAGAACATAAACCATAAAATGAGTGAC2840.13222463289041186No Hit
AACCCGGGAAATGCTGATTTGGCTTTATTATTTTTCTATTTGCATTATCAAT2640.12291303902488988No Hit
GACCTGGGGCATGAAATGCCACTGAAAAGAACTTTCAACACAGCATACTTCA2290.10661774976022645No Hit
ACTGACGGGACACCAAAATCGGACCTCCTTAAAGCATAAATCTCACAGGGCC2240.10428985129384598No Hit
ACAGCAGGTTTTGGGTGTCTTGGGCAAATCACTTCATGTCAGTGGACCTCAG2160.10056521374763717No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)