FastQCFastQC Report
Sat 31 Dec 2022
SRR5849216.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849216.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216280
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGC18100.8368781209543186No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCACTTATCTCGTATGCCGTCT17920.828555576105049No Hit
GCACATGGGGACTTTGGATTTGTTTTAGAACATAAACCATAAAATGAGTGAC3220.148881080081376No Hit
AACCCGGGAAATGCTGATTTGGCTTTATTATTTTTCTATTTGCATTATCAAT2400.1109672646569262No Hit
TGAATCGGGATTGCCTGAGCGTTTGGAAGACATTTGCTTTCCACTTTTCCCC2330.10773071943776585No Hit
TTACCAGGGTTGCCTTTTCTCCACATCCAAACCAATACTTGTCTTTTGTCTT2300.1063436286295543No Hit
GAACTAGGGCAAGTAAATTTGACATGCTTAACACTGGGAGTGATTATTTACC2300.1063436286295543No Hit
GGAAGATGGGGGTATTTTTTAAGCAATGAAAATTCAACTGAGTACAAAGCCC2290.10588126502681708No Hit
AAGATCGGGGCAACCAACATGATTCAAGCATTTTCTCTTAGTACACATCATC2280.10541890142407989No Hit
CTTCGCGGGATTTCAGATGGATGAGCTTCTGACTCTTTCTTAAAATTCTTTT2180.10079526539670798No Hit
GAACATGGGGGAAGAACACAAAGAGAAGGAATTAAACATTTTTATTATACAT2180.10079526539670798No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)