FastQCFastQC Report
Sat 31 Dec 2022
SRR5849203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172267
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGC8910.5172203614157094No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGCCGTCT8110.4707808227925256RNA PCR Primer, Index 47 (95% over 22bp)
CTTAGGGAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAAGTTAATG2330.13525515624002277No Hit
TTTGCTGGGATGTATGAAAAATTGCTGTTGAACATACTAACTCAATCTTTTC2110.12248428311864722No Hit
TCTTCAGGGATATGAGATCATGTCGTCTCCAGAGAGGGACAATTTTACTTTT2100.12190378888585743No Hit
TAACGAGAGGGAAGTGAGGAGAACGTTGAAGGGCTCAAAGAAGTAAAAGCAG2050.11900131772190845No Hit
TGTCATGGGGGCGCTGAGAACGCGGGTCCACGCGTGTGATCGTCCGTGCGTC1990.11551835232516965No Hit
GCTTAAGGGGTGATTGGCCCGCCTAGGCCTCCCAGGGTGCTGGGATTGCAGG1990.11551835232516965No Hit
GTGTTAGGGAAGAGAGGATTCTCCAGTCATGTGATTTGCTCATTAGTTAAGA1970.11435736385959006No Hit
CTGTCTGGGAAGAGGAACCTAGATTACAATAAGATAAACTCATACTATTTCA1970.11435736385959006No Hit
GAAAAAGGGAGACCTTGACTTTGAGAAGGTCATGCATACAAGGAAGAGGCAT1950.11319637539401045No Hit
GCTGCTGGGGATCTGTTAAAGGGTCACCTGGCATCTTACATCTGAAATAAGG1910.11087439846285127No Hit
GCGATAGGGAACTTAATACATGAATGTAATCCTCAGTTATTAAACAAGTGGG1900.11029390423006147No Hit
GTTGGTGGGCGCAGGAGGAACTTGAAAGGAGGGTAAAAAGACTAAAATGAGG1860.10797192729890229No Hit
CAAGTAGGGATTATTATTTGCTAAGAAATTCTGTCAGGGCTTTGCCTTCATA1850.10739143306611248No Hit
TTGCTAGGGATACTCACTTCCCATAAGATATTGTACCATTTACTCATTCAAT1810.1050694561349533No Hit
TACCAGGGGTTGTCTCTTGAGTGGTGTTGATTTAAAGATCTTTCTTGAGCTG1800.1044889619021635No Hit
TCATATGGGTGCACCAAAGAAAAGACTCATAATTGGTTGCATTTACCCCACA1790.10390846766937371No Hit
TTAACAGGGTGAAGGCTGAATCAAAGACATTTCATCCACCAATATCATGTGT1790.10390846766937371No Hit
AAGTTCGGGATGTTGTATTGTAAAATATATGCTGAACAGACTTTCTTACATC1780.1033279734365839No Hit
GTGCTTGGGCAACTGTGCCGCTTCAACAGAAAAAATCTATATCCGAAAGGAC1770.10274747920379411No Hit
ACGTTTGGGTGGTGAAAATATTGAAGCACTGGACAAGATGTTGGAAGTTCTG1760.10216698497100432No Hit
TTGCGCGGGAGAAGTAAGCTATTTGTTGACAATTTTAGAGTTAAATTTGACA1750.10158649073821452No Hit
ATCTTAGGGTCTATCACCAGATGGCAGTTGGGATATGGGAGGAACTAAAGCA1750.10158649073821452No Hit
AATTAGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAA1750.10158649073821452No Hit
CTAGCAGGGATATTCTAAAACCGTACTGGCCTAGATACTACAACTGAACTTT1740.10100599650542472No Hit
CATACAGGGTAAAGGACAAATGGTTGCATTCTTTTGAGTTTCTGATTAGCTT1740.10100599650542472No Hit
GGAAACGGGCACCACCACATATTTCTCAGGTCAAACAGTCTTATTACTTTCT1730.10042550227263491No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)