FastQCFastQC Report
Sat 31 Dec 2022
SRR5849202.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849202.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171040
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGC9030.5279466791393826No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGCCGTCT8050.4706501403180543RNA PCR Primer, Index 47 (95% over 22bp)
CTTAGGGAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAAGTTAATG2330.13622544434050515No Hit
GTGTTAGGGAAGAGAGGATTCTCCAGTCATGTGATTTGCTCATTAGTTAAGA2260.13213283442469598No Hit
TCTTCAGGGATATGAGATCATGTCGTCTCCAGAGAGGGACAATTTTACTTTT2060.12043966323666978No Hit
GTGCTTGGGCAACTGTGCCGCTTCAACAGAAAAAATCTATATCCGAAAGGAC2060.12043966323666978No Hit
TTTGCTGGGATGTATGAAAAATTGCTGTTGAACATACTAACTCAATCTTTTC2040.11927034611786717No Hit
TGGCGAGGGGGATCTGATCCGTTTACAGCACCAGACGGAACTGGAAAACCAG2020.11810102899906455No Hit
GAAAAAGGGAGACCTTGACTTTGAGAAGGTCATGCATACAAGGAAGAGGCAT2020.11810102899906455No Hit
TAACGAGAGGGAAGTGAGGAGAACGTTGAAGGGCTCAAAGAAGTAAAAGCAG2010.11751637043966323No Hit
TTATGAGGGGAGAGAAAGGATTTGGCTACAAAAACAGCAAATTCCATCGTGT1970.11517773620205801No Hit
CTGGCTGGGTGGCCCTGGCTGGAGGCCTTGCTTTGAGGGGCTGTGACCCTCT1930.11283910196445276No Hit
GCTTAAGGGGTGATTGGCCCGCCTAGGCCTCCCAGGGTGCTGGGATTGCAGG1870.10933115060804491No Hit
TATATTGGGCGATCTTCAATCGGGGAAGGACAGGCCCCGGCCTTGGGAGAGG1870.10933115060804491No Hit
AAGAGGGGGCTGACACTAAGAGGTAAGGTGTCATTCTGTACTATTTTGAGTA1850.10816183348924227No Hit
TTCAGCGGGCTTGTGAAATCGTGCATGAGGCCCCAGGGCACCGTTCTAGAAC1830.10699251637043966No Hit
TGTCATGGGGGCGCTGAGAACGCGGGTCCACGCGTGTGATCGTCCGTGCGTC1820.10640785781103836No Hit
CTGTCTGGGAAGAGGAACCTAGATTACAATAAGATAAACTCATACTATTTCA1820.10640785781103836No Hit
TGCCAAGGGAGCAGCCGGGCCGTGGTCTGGCTGAAGCTGCGGGGCCGCGGGG1810.10582319925163705No Hit
AAGAGAGGGCTGCATTTATTGTGATTTTTGGAAACAGGTATCAACCTTCACA1810.10582319925163705No Hit
TGGAAAGGGAACTTCAGTAAAATGGCAGAAGAGAAACTGACCCACAAAATGG1810.10582319925163705No Hit
ATAGTAGGGGGGTTCTTAAATCTCATGAACCAGTCTGCTAGCTTTAAGCTTA1800.10523854069223575No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCCTGTCTCTTATAC1790.10465388213283441No Hit
ACGTTCGGGGATCTTTCCAAGGGCACTTATATCTCCAGGTTCTTTCATTGAG1790.10465388213283441No Hit
AATTAGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAA1780.10406922357343311No Hit
CATACAGGGTAAAGGACAAATGGTTGCATTCTTTTGAGTTTCTGATTAGCTT1780.10406922357343311No Hit
CAGCTTGGGCTATTTCTCAATCAAGAAATTTTACAAGACAACCAAGCAAACT1770.1034845650140318No Hit
GTTGTTGGGGGGAAAGAAATGCGTTTAAATCACAGAGCAGTGTATAGGAATT1770.1034845650140318No Hit
TAAATAGGGGAAAGAAAGGAGGGAAATTGAGAGATGGGGAAAAAATTGGAAT1750.1023152478952292No Hit
ACGTTTGGGTGGTGAAAATATTGAAGCACTGGACAAGATGTTGGAAGTTCTG1740.10173058933582786No Hit
TTGCTAGGGATACTCACTTCCCATAAGATATTGTACCATTTACTCATTCAAT1730.10114593077642656No Hit
TACCAGGGGTTGTCTCTTGAGTGGTGTTGATTTAAAGATCTTTCTTGAGCTG1730.10114593077642656No Hit
GCACAAGGGCCTTTTTCCCACCCCCTAGCGCCGCTGGGCCTGCAGGTCTCTG1720.10056127221702525No Hit
GCACAAGGGATTCACTGTAGAGATAAGCAAATCAAATAGAACAGTGACAAAA1720.10056127221702525No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)