FastQCFastQC Report
Sat 31 Dec 2022
SRR5849201.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849201.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174268
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGCCGTCT8260.47398260151031746RNA PCR Primer, Index 47 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGC8080.4636536828333372No Hit
GAAAAAGGGAGACCTTGACTTTGAGAAGGTCATGCATACAAGGAAGAGGCAT2340.13427594280074368No Hit
TTTGCTGGGATGTATGAAAAATTGCTGTTGAACATACTAACTCAATCTTTTC2170.1245208529391512No Hit
GTGTTAGGGAAGAGAGGATTCTCCAGTCATGTGATTTGCTCATTAGTTAAGA2150.12337319530837561No Hit
TAACGAGAGGGAAGTGAGGAGAACGTTGAAGGGCTCAAAGAAGTAAAAGCAG2030.11648724952372208No Hit
CTTAGGGAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAAGTTAATG2030.11648724952372208No Hit
CTGTCTGGGAAGAGGAACCTAGATTACAATAAGATAAACTCATACTATTTCA2010.1153395918929465No Hit
CATACAGGGTAAAGGACAAATGGTTGCATTCTTTTGAGTTTCTGATTAGCTT2010.1153395918929465No Hit
GACATAGGGATGTGAAAATCACAGATTTTGGGCTAGCCAGACTCTTGGAAGG1970.11304427663139532No Hit
TTGCGCGGGAGAAGTAAGCTATTTGTTGACAATTTTAGAGTTAAATTTGACA1950.11189661900061973No Hit
TCTTCAGGGATATGAGATCATGTCGTCTCCAGAGAGGGACAATTTTACTTTT1910.10960130373906857No Hit
AATTAGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAA1910.10960130373906857No Hit
ATTGGCGGGACTGGGCATGAAGCTTAGATGTACCTTGAACTTGAAATGATCT1910.10960130373906857No Hit
AGTAACGGGAAGTCCTTGAGGAAGGGGCTGCCAGTGAAAGAGTCAAGATGGA1910.10960130373906857No Hit
GCAGCTAGGGGCAGGGATCTATTCATTATTGGTTCATTCCTGTGATGATAAG1900.10902747492368076No Hit
TTACATGGGATATCAAAATGACTCATCATCTAATGATGCTTGGGTTAGGTTG1890.10845364610829297No Hit
AGCTGTGGGGGAGCTGCAAGCGGCAGGTGCGGGAGATCGAGAAGCTGCTGCA1840.105584502031354No Hit
TTCAGCGGGCTTGTGAAATCGTGCATGAGGCCCCAGGGCACCGTTCTAGAAC1840.105584502031354No Hit
TAAATAGGGGAAAGAAAGGAGGGAAATTGAGAGATGGGGAAAAAATTGGAAT1830.10501067321596622No Hit
GACGTGGGCAGTTACAATGAGTATCTTCCCAGTCTTGTTATTCTCTTTGCTT1820.10443684440057842No Hit
TACCAGGGGTTGTCTCTTGAGTGGTGTTGATTTAAAGATCTTTCTTGAGCTG1810.10386301558519062No Hit
TAGTCTGGGATTGTATCTGTTTTCTACTTTATGTGAGCAAGGTTTGCTGTCC1800.10328918676980284No Hit
CAATTAGGGATGCAAAATAGAAAAAAAATGTTCAACATTTATTGATTGATAG1790.10271535795441504No Hit
GTTGGTGGGCGCAGGAGGAACTTGAAAGGAGGGTAAAAAGACTAAAATGAGG1780.10214152913902724No Hit
GTAGGTGGGACAGGTGATTTAGTAAATTTAGACAGTAGTTAAACTCTTGTTA1780.10214152913902724No Hit
GAAACTGGGTGGAACCACAGCTCTGGGAACTGGGAAAAGTGCTTCGGAAGGC1770.10156770032363945No Hit
GTGAAGGGGTCTCTTTGTAGTATGTCAGTTGCACTTTTTTTCTGCCATAATG1770.10156770032363945No Hit
TGACAAGGGGGGGCAGAGGGTGTATTTTTAAGACCTGGTGGTTCTGTTTCTC1770.10156770032363945No Hit
TCATATGGGTGCACCAAAGAAAAGACTCATAATTGGTTGCATTTACCCCACA1760.10099387150825166No Hit
GTGCTTGGGCAACTGTGCCGCTTCAACAGAAAAAATCTATATCCGAAAGGAC1750.10042004269286388No Hit
TACAATGGGTGGCGGAGGGTGAAGCAGGAATAGGCACGTCATATGGCCAGAG1750.10042004269286388No Hit
TGGAAAGGGAACTTCAGTAAAATGGCAGAAGAGAAACTGACCCACAAAATGG1750.10042004269286388No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)