FastQCFastQC Report
Sat 31 Dec 2022
SRR5849173.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849173.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102601
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGCCGTCT8650.8430717049541427RNA PCR Primer, Index 47 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGC8490.8274773150359158No Hit
GGGCTTGGGACAGAAATCCAAAGCTTTGCTGTCATCACATAAATGAATTAAA1780.17348758784027446No Hit
TGAAGTGGGCAGAAAGTCTGCAAATATGGGCAGTTTTATTTCTTTTTTTTTT1670.16276644477149346No Hit
TTAATCGGGTAGCAACTTAATCTGTAAAGCATTTGAGAATAAATCTGAAATA1550.15107065233282327No Hit
ATAACTGGGGTGGCAATTTTTAAAAAATAAGACAACAATGAAGTTTGCCCTA1520.14814670422315573No Hit
GCATCAGGGAGAACACACCTCCTTAAAAAGCATATACTTGTGATGAATTTTG1520.14814670422315573No Hit
TACGTTGGGCCCGTGATAGATTATTGATAGCAATAGAGAGGGGCGGCTTTGT1470.1432734573737098No Hit
TTCAAGGGGATGCCCTTCAGTGAACAGTTGTATAAACAATAATTATAATTTG1460.14229880800382064No Hit
GGCTGCGGGTAAAGCCAGAATCCTGCTTTATAGCCAGTGGTATGCTGGTACT1390.13547626241459634No Hit
CCAAGCGGGTGAGCAGCTAAAGTAATTTCCACTTTTATGATTGCTGGCTAGT1390.13547626241459634No Hit
AAACTTGGGCAGTCTGTTAGTTTTCTTTTTGCTTGTTTATCTATGCAAACAG1390.13547626241459634No Hit
CATTTCGGGTATGTGACAAAGGTGGCATAGCCCATCATGAGGAAAAGGGGAG1350.1315776649350396No Hit
ATAGTTGGGGAAGAACGCAAAGCTGAGAACATGGACGTTAATATCGCCCCAC1330.12962836619526125No Hit
GGTCAGGGCAGACAGAAAGTTTTTAAAAAATGATGATTTAAGCATTGAAAAA1290.12572976871570454No Hit
ACAGCGGGGCAGCACTAGACAGGTCATCAAGACAAAAAGTCAACAAAGAAAC1290.12572976871570454No Hit
TCTTGCGGGACCCCATTGGTTGTTAGCCAATAGTAGTCACAGTTCAAAGATG1250.12183117123614778No Hit
GTTAAGGGGGACCTTGAAACTAAAGGAAACTAATCAGCCCACTTTCAAAATT1250.12183117123614778No Hit
ACCACTGGGATAGCTGAAGTCATCATGTAGCTGGGATATTCTATAAACATTT1230.11988187249636943No Hit
AGTGACGGGATTTTTGTATGGCTCTAAGTTAAGGAAGCTTTTTATATTTCTA1220.11890722312648025No Hit
TATCGCGGGAATGTACCTCTTTAACTTTTTTCTAGCACTTATTTCATTTAGA1220.11890722312648025No Hit
TTAGCCCGGGCATAGAGATTATATTGTATCAGTGTTTATGTAAGCTGGAATC1220.11890722312648025No Hit
CGACATGGGAGCTCTAACATCTGACCTCATTTCCTGACATCCTGGCCCTTTG1220.11890722312648025No Hit
ACTGAGGGGTGATACAGCTCTAGAATTTCGTGAAGTTGCATGCAAAGTTGCA1210.11793257375659107No Hit
TTTCTTGGGAAGGCCACTCATAGGGGCATCCATCCCCAAGCACCTCCTCATG1210.11793257375659107No Hit
TGCAGTGGGAACCAAAGGGGTGACCAGCCCTCAGATGTGCTTATTGGATTCC1210.11793257375659107No Hit
CCATCCGGGACAGCTTTGATTGATACATTGTTGAGAAAATAACATTTACCAA1210.11793257375659107No Hit
GACTAGCGGGACTTGACAGTCTTTATTACATCATTCTCAGCTAGTCTGAGTT1210.11793257375659107No Hit
TAGGCTTGGGGGGATCAATGTAGCAAGAAGAGCTAACTATATATATGCACCC1210.11793257375659107No Hit
TCGCATGGGGAATCTTCCAATTTTATCTATTTGATTCAAATGGTATGTTTTT1190.1159832750168127No Hit
CTACGGGGGCGATCTTTGCCGGGGAAACCAAAAGCTGGTTCTTTGAGAAGCT1190.1159832750168127No Hit
GAAAATGGGGTGGGCGGTTTCTGGGGATGTCCCAGGGTGGAACTCCACATGC1190.1159832750168127No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCCTGTCTCTTATAC1180.11500862564692352No Hit
AAGACGGGCAGAAAAGAATATGAGCTATGTCACTGTTTTGTATGTGGGGAGT1180.11500862564692352No Hit
CGCTTCGGGCTGTGGATAATCTTGGTAAGTAATTTGGGTTTAAAACATTTTT1170.11403397627703434No Hit
CATCACGGGACTTCCCAATACATCAGAAAAGCTCTTTCTCTTTTTGTGTCAG1160.11305932690714515No Hit
TATCACGGGAATTTGCAAAGAGCTTCAAACACCAAAAATATACCATTTTAAG1160.11305932690714515No Hit
TACCTGGGGGGCGCATGCTCAGGCGGGGATGTGTGCGAAGCCTGCCGCTGCT1160.11305932690714515No Hit
TTGCTTGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGG1160.11305932690714515No Hit
GAGGCGGGAGAATGGCGTGAGCCCGGGAGGCGGAGCTTGCGGTGAGCCGAGA1160.11305932690714515No Hit
CTACTTAGGGGATGATATGGAATGGCCAACTTATTCTAGAAAATTTACATTT1150.11208467753725598No Hit
ACGTGCGGGCATTTGCCAACAAGTTTATCCTACTGCACTTCATTTTCATTCC1140.1111100281673668No Hit
AATAGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1140.1111100281673668No Hit
TGTTCGGGGAGCATAAAGACGGAACACAACAAATCCTTCCTGAAGTGAATTA1130.11013537879747762No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCCTGTCTCT1130.11013537879747762No Hit
AAACTGAGGGAACTGCCAACGAGATGCTTTAAAAATATTCCCTTTTGTATAG1120.10916072942758843No Hit
CGTACCGGGCAGACTCGATGGAGAGCTTCCCAGAGTCCCTCTCTGCCTTAGT1120.10916072942758843No Hit
AGTCATGGGAAGAGTCAAGAAGTAACTCCAGCTTGTCTGGATGGAAAAAGCT1120.10916072942758843No Hit
ATAGTTGGGATGGTCACAAACTAGACTCTATAGAAGGAAAGCAACTTGATTA1120.10916072942758843No Hit
AAGTGCGGGAGGATCTTTTTTTGCAAACAACTGATATATGCAGACAAATTTT1110.10818608005769925No Hit
CTTAAAGGGATCCAGATTGGGTGGGGAAGAAAAGGCTTTCTGAAAGAATGAG1100.10721143068781006No Hit
TAGCCAAGGGTAGGAGAAAGAGATACGAAGTACTGGCAATACAATTAGAAAA1100.10721143068781006No Hit
CACAGCGGGAATTAAATGGTGATTAAAAATTTATCTCCTTTAAATCTAAAAA1090.10623678131792087No Hit
CGCCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1090.10623678131792087No Hit
TGTCTAGGGATAAAGACACATGTACACTGAAAATAAAAGGATGGAAGAATAT1090.10623678131792087No Hit
CAGAAGGGGATGCATAGAAGTGGAAAGTAGGGTTTCTCATTTTAAGGTATTT1090.10623678131792087No Hit
CTTATGGGGGTAGCCTCCCTGGGCTGATCATTCCCTCTCCTTGTAATTGCAG1090.10623678131792087No Hit
ATTTAAGGGCTACCTGGCAAGGAATGCAGCAGTATCCAGGAGCCCAGAGCAA1090.10623678131792087No Hit
TAGGCGGGGATCCCCATCAGCCTTTTGCAGGTGAAGTACAGAGCCCAGATCT1090.10623678131792087No Hit
CTCAGCGGGCATTTGCCCAATGACAAATAGCTGGTATGTTGCAGAGCCAGAA1090.10623678131792087No Hit
GTCAATGGGGGACAAGTGTGTGTGCCTATTGACCGCCTTTTCCGAGCGCATG1080.10526213194803169No Hit
ATTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1080.10526213194803169No Hit
GCGACCGGGAAAGAAAATCAGAGAGTGATAAAGATGTAAAAGTTACACGGGA1080.10526213194803169No Hit
ACATGTGGGGCCTAAAGAAATTTTTCAGTTATTTGTCTCAGAAAGTATATCT1080.10526213194803169No Hit
CCTAGTGGGGGAACCATATTTGGGTTCTAAATGTAAATCAATGTCAATAATA1080.10526213194803169No Hit
TAGCTCTGGGGGTTACACGAGGTCTGAGAGACAGAGGCAGCGTGTTTGAGCT1070.1042874825781425No Hit
GTTCGAGGGCATGTTGGGCTGCACTGCCAGCACTGACATGACGGGAACCTGG1070.1042874825781425No Hit
TACTTAGGGGATCTGCGAGCGGGGACTGTGGAACTATTTTACCTTAAGACCT1070.1042874825781425No Hit
GCGCGGGGGGATAGGACATTAACAACGGTTACAATGTCGCTAGGTGACAGAA1070.1042874825781425No Hit
CAGTGGAAATTGGATCATAAGCCAGTGTCCTAAAGGATGAATAGGAATTAGC1060.10331283320825332No Hit
CATCTTGGGGACCAGCTCCCCGCTGCCCTGCGCTCGGCGCGCTGGCATCCGG1060.10331283320825332No Hit
TCTAATGGGGAACGTTGATTTGACTTTTTAAAGGTTTTTGGTCAAGTTTCCT1060.10331283320825332No Hit
CGTGGGGGGATCACCAGCTGACCACTAAGATAACAGAACAGAGACTTCCATG1060.10331283320825332No Hit
TCCAGTGGGGCCTCATGCAAGCCCATTGTCCCTGTCCAACACACCCCCACAT1060.10331283320825332No Hit
TGGTGTGGGAACCTAACATATTCACAGTGGGCTGTGTACAGAGTCAGGTGAC1060.10331283320825332No Hit
CATCATGGGTGCTCATGGGTAGGAAAATCAATATCATTAAAATGGCCATACT1050.10233818383836414No Hit
AGGATTGGGAGAGGGATTGCAGATGGGAGCTGTCCACATACCGAAGTGCTGA1050.10233818383836414No Hit
CTAGTAGGGCATTTTTCCTTGTATTTTTAGAGCGAGGCTTCCTTCATCTCCT1050.10233818383836414No Hit
CGCAGAGGGTTGAAAGCAGATTTTCCCCTTAATCCTCAACCAAATAAAATGA1050.10233818383836414No Hit
TGGTGAGGGAGACAAAAAAGAAGAAAACTACCTTCTGATCTTGTATTTTGAA1050.10233818383836414No Hit
CAAATAGGGTGTACTTCATTAGCATTTAACACCTATACTGTAATTTTAGAAA1050.10233818383836414No Hit
GATATAGGGGGTTCCGGGCGCTTGGAGAAGATGGTGCTGCGGCGGCTGCTGG1040.10136353446847496No Hit
ACAGCAGGGAGGCAAGCATACACAGGCACATGGCTTTAAGAACCACACTGAT1040.10136353446847496No Hit
CGTACAGGGGTTGTAACTACCTTATAAAAAAAAGAGGCCATTATAAGTGATC1040.10136353446847496No Hit
AAACTAGGGGAATAAGAATAAAATCTGAGTTGAGTAGTTTTTACTGTGTTTT1040.10136353446847496No Hit
ATCTTTGGGGTAGACTGCCATTATGTGTGGTCTTCCCATTAAATGTGAACAT1030.10038888509858579No Hit
TCGACTGGGCAATAAAAGATAAACCATAGACTTGGAGTCAGTCTTTATAATA1030.10038888509858579No Hit
CTGGACAAGTGCTTCAGAAGAAGTCTACCTTTATCTGAATGATAAGCGCCTT1030.10038888509858579No Hit
CGTGGTGGGAGTGTAAATTAGTTCAACCATTGTGGAATACAGTGTGGCGATT1030.10038888509858579No Hit
GCTTGCGGGAGAAGTCTCTAGGATCTGCATATAAACAATAAATGTTTCCTAG1030.10038888509858579No Hit
GCACCTGGGGAGGAACACAATCAAGGTGCCACTGGCCATTTCTTGGGTCCCT1030.10038888509858579No Hit
AAGTTAGGGGGGATCTGCTCACTTCTGGTCTTTTTGGCCGGGAACTCCTGAT1030.10038888509858579No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)