FastQCFastQC Report
Sat 31 Dec 2022
SRR5849172.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849172.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101488
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGCCGTCT8610.8483761626990383RNA PCR Primer, Index 47 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGC8190.8069919596405486No Hit
GGGCTTGGGACAGAAATCCAAAGCTTTGCTGTCATCACATAAATGAATTAAA1840.18130222292290712No Hit
TGAAGTGGGCAGAAAGTCTGCAAATATGGGCAGTTTTATTTCTTTTTTTTTT1660.16356613589784014No Hit
TTCAAGGGGATGCCCTTCAGTGAACAGTTGTATAAACAATAATTATAATTTG1540.1517420778811288No Hit
TGTTCGGGGAGCATAAAGACGGAACACAACAAATCCTTCCTGAAGTGAATTA1410.1389326816963582No Hit
ATAGTTGGGGAAGAACGCAAAGCTGAGAACATGGACGTTAATATCGCCCCAC1380.13597666719218038No Hit
TCTTGCGGGACCCCATTGGTTGTTAGCCAATAGTAGTCACAGTTCAAAGATG1360.1340059908560618No Hit
AGTGACGGGATTTTTGTATGGCTCTAAGTTAAGGAAGCTTTTTATATTTCTA1350.13302065268800253No Hit
TTAATCGGGTAGCAACTTAATCTGTAAAGCATTTGAGAATAAATCTGAAATA1350.13302065268800253No Hit
AAGTGCGGGAGGATCTTTTTTTGCAAACAACTGATATATGCAGACAAATTTT1340.13203531451994324No Hit
TGCATAGGGATAAGGTATTTATTCAGCCTTTTATAACTCTAGGAGTTGAAGT1340.13203531451994324No Hit
ACAGCGGGGCAGCACTAGACAGGTCATCAAGACAAAAAGTCAACAAAGAAAC1330.13104997635188395No Hit
TACCTGGGGGGCGCATGCTCAGGCGGGGATGTGTGCGAAGCCTGCCGCTGCT1330.13104997635188395No Hit
TAGGCTTGGGGGGATCAATGTAGCAAGAAGAGCTAACTATATATATGCACCC1330.13104997635188395No Hit
CGACATGGGAGCTCTAACATCTGACCTCATTTCCTGACATCCTGGCCCTTTG1320.1300646381838247No Hit
GAGGCGGGAGAATGGCGTGAGCCCGGGAGGCGGAGCTTGCGGTGAGCCGAGA1310.12907930001576542No Hit
CCAAGCGGGTGAGCAGCTAAAGTAATTTCCACTTTTATGATTGCTGGCTAGT1290.12710862367964684No Hit
CATCATGGGTGCTCATGGGTAGGAAAATCAATATCATTAAAATGGCCATACT1270.1251379473435283No Hit
GGCTGCGGGTAAAGCCAGAATCCTGCTTTATAGCCAGTGGTATGCTGGTACT1260.12415260917546901No Hit
ATAACTGGGGTGGCAATTTTTAAAAAATAAGACAACAATGAAGTTTGCCCTA1260.12415260917546901No Hit
TATCGCGGGAATGTACCTCTTTAACTTTTTTCTAGCACTTATTTCATTTAGA1250.12316727100740975No Hit
TCTCACGGGATGTGTGTGTATGTGTAAAAATGTATTCTGAAAAGGAAAAAAT1240.12218193283935047No Hit
AAACTTGGGCAGTCTGTTAGTTTTCTTTTTGCTTGTTTATCTATGCAAACAG1240.12218193283935047No Hit
ACATGTGGGGCCTAAAGAAATTTTTCAGTTATTTGTCTCAGAAAGTATATCT1240.12218193283935047No Hit
CATTTCGGGTATGTGACAAAGGTGGCATAGCCCATCATGAGGAAAAGGGGAG1230.12119659467129118No Hit
TAGCCAAGGGTAGGAGAAAGAGATACGAAGTACTGGCAATACAATTAGAAAA1230.12119659467129118No Hit
CATCTTGGGGACCAGCTCCCCGCTGCCCTGCGCTCGGCGCGCTGGCATCCGG1220.1202112565032319No Hit
TTGCTAGGGACAGGTAACTGTTTAATCCCTTACAATTTTAAATGAGGTATTT1220.1202112565032319No Hit
CGTACCGGGCAGACTCGATGGAGAGCTTCCCAGAGTCCCTCTCTGCCTTAGT1210.11922591833517264No Hit
CTTAAAGGGATCCAGATTGGGTGGGGAAGAAAAGGCTTTCTGAAAGAATGAG1200.11824058016711335No Hit
TAGGCGGGGATCCCCATCAGCCTTTTGCAGGTGAAGTACAGAGCCCAGATCT1200.11824058016711335No Hit
ACTCCAGGGATGTGTTTTCTCCTCTTTGGTTCTCTTTGTATTTACTACTTTT1180.1162699038309948No Hit
TCGCATGGGGAATCTTCCAATTTTATCTATTTGATTCAAATGGTATGTTTTT1170.11528456566293553No Hit
TTAGCCCGGGCATAGAGATTATATTGTATCAGTGTTTATGTAAGCTGGAATC1170.11528456566293553No Hit
CTAGTAGGGCATTTTTCCTTGTATTTTTAGAGCGAGGCTTCCTTCATCTCCT1170.11528456566293553No Hit
AATAGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1170.11528456566293553No Hit
CTACGGGGGCGATCTTTGCCGGGGAAACCAAAAGCTGGTTCTTTGAGAAGCT1170.11528456566293553No Hit
GCATCAGGGAGAACACACCTCCTTAAAAAGCATATACTTGTGATGAATTTTG1160.11429922749487623No Hit
CATCACGGGACTTCCCAATACATCAGAAAAGCTCTTTCTCTTTTTGTGTCAG1150.11331388932681696No Hit
ACTCCTGGTACGTTGAGACCCTGGACGACATCTTTGAGCAGTGGGCTCACTC1150.11331388932681696No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCCTGTCTCTTATAC1150.11331388932681696No Hit
CATCTAGGGGAATGGGAAACAGATTCCAAATACATTCATGAAAAGAGTAACA1150.11331388932681696No Hit
TATTCGGGGGAGATCAAGGTTTGCAGGATTTGCAAAAGCCTTGATTTGAAAG1140.11232855115875769No Hit
TCGACTGGGTAATGGTAAAGGGATCAATTCAACAAGAAGAGCTAACTATCCT1140.11232855115875769No Hit
ACTACGGGGGGGAGAAAGATGGCGGCTACCTTACTAGCTGCTCGGGGAGCCG1140.11232855115875769No Hit
GGTCAGGGCAGACAGAAAGTTTTTAAAAAATGATGATTTAAGCATTGAAAAA1120.11035787482263912No Hit
GTTAAGGGGGACCTTGAAACTAAAGGAAACTAATCAGCCCACTTTCAAAATT1120.11035787482263912No Hit
TAAGCCGGGGATGCAGGAGAAATAAGTAGAAGCCAAGTGAGGAGGGCCTTGA1120.11035787482263912No Hit
AGTGTTGGGTCTGATATTTTACAAACCTGACAAAATGAGCAATGGGGAAAGG1110.10937253665457985No Hit
TAGCAGGGGGAAAGTAAATCAGACAAGATTCCCTAACTGCCTGAATGAAAGA1110.10937253665457985No Hit
TGTTTCGGGTGGATATATCATAATTTACTTAACAATTCTTGTTTTATTGGGC1110.10937253665457985No Hit
GGAAGAAATGAAGAGTGGACTGAAAGATTTCAAAACATTTTTGACAAATGAT1100.10838719848652058No Hit
AAACTGAGGGAACTGCCAACGAGATGCTTTAAAAATATTCCCTTTTGTATAG1100.10838719848652058No Hit
CACAGCGGGAATTAAATGGTGATTAAAAATTTATCTCCTTTAAATCTAAAAA1090.10740186031846129No Hit
GATATAGGGGGTTCCGGGCGCTTGGAGAAGATGGTGCTGCGGCGGCTGCTGG1090.10740186031846129No Hit
GACCTTGGGATATCTGAGGCCAGTAACTATTAATATCTAGTTCTCAGAGCAT1090.10740186031846129No Hit
TATCACGGGAATTTGCAAAGAGCTTCAAACACCAAAAATATACCATTTTAAG1090.10740186031846129No Hit
AAGTTAGGGGGGATCTGCTCACTTCTGGTCTTTTTGGCCGGGAACTCCTGAT1090.10740186031846129No Hit
GTCAATGGGGGACAAGTGTGTGTGCCTATTGACCGCCTTTTCCGAGCGCATG1080.10641652215040201No Hit
AGTACTGGGAGGCGTGAGCCACTGCGCTTGGCCTCATGTAATATTTTTGTAT1070.10543118398234275No Hit
TTACGAGGGGTGGAATTGGGGTATGGGGAAGGCATGATTGCATAAAGATCAT1070.10543118398234275No Hit
CGCTTCGGGCTGTGGATAATCTTGGTAAGTAATTTGGGTTTAAAACATTTTT1070.10543118398234275No Hit
TGTAATGGGATGGTCATTTTAATGATATTGATTCTTCGAATCCATGAGCACG1070.10543118398234275No Hit
CTTATGGGGGTAGCCTCCCTGGGCTGATCATTCCCTCTCCTTGTAATTGCAG1070.10543118398234275No Hit
CGAAATGGGCAGGGAGGGACCCACCAGAGCACAAGAGAAGGTGGCTACCTGG1070.10543118398234275No Hit
GTGACTGGGACACTAGCCAATCCTCAGCCTGTGGAGCCTGCCTCACCCATTC1070.10543118398234275No Hit
TAGTACGGGTCCTGGCCTGAAGTGAACCCTGTTTCTAAAAAACACAAACTAC1060.10444584581428347No Hit
ATTTAAGGGCTACCTGGCAAGGAATGCAGCAGTATCCAGGAGCCCAGAGCAA1060.10444584581428347No Hit
AGAAAGGAACTCTCTTTCTTCTCTTGGCACTGGGGGCGTCAGGCTGTCGGAT1060.10444584581428347No Hit
CGCAGAGGGTTGAAAGCAGATTTTCCCCTTAATCCTCAACCAAATAAAATGA1060.10444584581428347No Hit
TACGTTGGGCCCGTGATAGATTATTGATAGCAATAGAGAGGGGCGGCTTTGT1060.10444584581428347No Hit
GCGACCGGGAAAGAAAATCAGAGAGTGATAAAGATGTAAAAGTTACACGGGA1060.10444584581428347No Hit
GCAAAAGGGTGACCGACTACCTCATGAAGATCCTCACTGAGCGAGGCTACAG1050.10346050764622418No Hit
TTTGCAGGGATGGAAGCCAAAGTGAAGGCTCAGACTGCTCCAAACAAGGATG1050.10346050764622418No Hit
ACCTTGGGGTCACATGGTCATCGTAGCAATGCTGTGAACTCCTTACTGCTTT1050.10346050764622418No Hit
CGTACAGGGGTTGTAACTACCTTATAAAAAAAAGAGGCCATTATAAGTGATC1050.10346050764622418No Hit
GAGCTTGGGCCACTGGACACTGTTTGAAAGTTTGTAGTTTATAATATTCTTA1050.10346050764622418No Hit
AAGACGGGCAGAAAAGAATATGAGCTATGTCACTGTTTTGTATGTGGGGAGT1050.10346050764622418No Hit
TAGCTCTGGGGGTTACACGAGGTCTGAGAGACAGAGGCAGCGTGTTTGAGCT1040.1024751694781649No Hit
TGTCTAGGGATAAAGACACATGTACACTGAAAATAAAAGGATGGAAGAATAT1040.1024751694781649No Hit
CATCTGAGGGATAAAAATAAATGTTTTAATTTACTTAGAGCTGTATTCAAGT1040.1024751694781649No Hit
TGCAGTGGGAACCAAAGGGGTGACCAGCCCTCAGATGTGCTTATTGGATTCC1040.1024751694781649No Hit
GCGGGGGTTTTTTGATGGTGTTGACCAATAAAATATCTAGTGATAAGGAAAT1040.1024751694781649No Hit
GGGATGGGAGGTTGAAAGTAAAATGATAGAAAAAGATATACCATGCAAACAG1040.1024751694781649No Hit
GAAAATGGGGTGGGCGGTTTCTGGGGATGTCCCAGGGTGGAACTCCACATGC1040.1024751694781649No Hit
GCACCTGGGGAGGAACACAATCAAGGTGCCACTGGCCATTTCTTGGGTCCCT1040.1024751694781649No Hit
GCAATGGGGGCGCAATTATAGATCTGGATTTTGAACCACTTAATGAAGCGGC1030.10148983131010564No Hit
ACGTGCGGGCATTTGCCAACAAGTTTATCCTACTGCACTTCATTTTCATTCC1030.10148983131010564No Hit
CAACGCGGGGCACAATTTTGATTGTGATGAGTCACTTTCTGTAAACAATCTT1030.10148983131010564No Hit
CGTGGGGGGATCACCAGCTGACCACTAAGATAACAGAACAGAGACTTCCATG1030.10148983131010564No Hit
TCCAGTGGGGCCTCATGCAAGCCCATTGTCCCTGTCCAACACACCCCCACAT1030.10148983131010564No Hit
GCATCCGGGGTAGACCTGATCAAAATATCACCTAGTTGAAATCACTTTAGTA1030.10148983131010564No Hit
GCTTGCGGGAGAAGTCTCTAGGATCTGCATATAAACAATAAATGTTTCCTAG1030.10148983131010564No Hit
AACCTAGGGGCACTCTGTGGGGTACGTTTTATATCTTTTGGAATTAGCTAGT1030.10148983131010564No Hit
GACCGTGGGAATTTAATTTAAAGATCAATAGACAAATGAAACGTAAAAACAA1030.10148983131010564No Hit
TGTTAGAAATGCCCCACACAGACCTACTGAGCTAGAATTCACATTTAACAAG1020.10050449314204635No Hit
TGTATAGGGGCAAGAGGCTTCACCTTGAAAGGTAAAAAATTAACAACAGATA1020.10050449314204635No Hit
TATCCAGGGATGTAAATTTTAGTAACTCAGTCTCTCCCTTTTAAAAGGTATC1020.10050449314204635No Hit
GAGTATGGGGATCAACCTGGAGCTCTACGCCTCCTACGTTTACCTGTCCATG1020.10050449314204635No Hit
TACTTAGGGGATCTGCGAGCGGGGACTGTGGAACTATTTTACCTTAAGACCT1020.10050449314204635No Hit
CTCAGCGGGCATTTGCCCAATGACAAATAGCTGGTATGTTGCAGAGCCAGAA1020.10050449314204635No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)