FastQCFastQC Report
Sat 31 Dec 2022
SRR5849171.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849171.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100738
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGCCGTCT8630.8566777184379281RNA PCR Primer, Index 47 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGC8590.8527070221763386No Hit
GGGCTTGGGACAGAAATCCAAAGCTTTGCTGTCATCACATAAATGAATTAAA1900.18860807242549982No Hit
ACAGCGGGGCAGCACTAGACAGGTCATCAAGACAAAAAGTCAACAAAGAAAC1630.1618058726597709No Hit
TTAATCGGGTAGCAACTTAATCTGTAAAGCATTTGAGAATAAATCTGAAATA1600.15882785046357878No Hit
ATAACTGGGGTGGCAATTTTTAAAAAATAAGACAACAATGAAGTTTGCCCTA1530.15187913200579722No Hit
TTCAAGGGGATGCCCTTCAGTGAACAGTTGTATAAACAATAATTATAATTTG1470.145923087613413No Hit
CGCTTCGGGCTGTGGATAATCTTGGTAAGTAATTTGGGTTTAAAACATTTTT1390.13798169509023406No Hit
TGAAGTGGGCAGAAAGTCTGCAAATATGGGCAGTTTTATTTCTTTTTTTTTT1370.13599634695943935No Hit
TGTTCGGGGAGCATAAAGACGGAACACAACAAATCCTTCCTGAAGTGAATTA1350.13401099882864462No Hit
CTACGGGGGCGATCTTTGCCGGGGAAACCAAAAGCTGGTTCTTTGAGAAGCT1310.13004030256705512No Hit
TGGTGAGGGAGACAAAAAAGAAGAAAACTACCTTCTGATCTTGTATTTTGAA1300.12904762850165777No Hit
TATCGCGGGAATGTACCTCTTTAACTTTTTTCTAGCACTTATTTCATTTAGA1260.12507693224006827No Hit
TAGGCGGGGATCCCCATCAGCCTTTTGCAGGTGAAGTACAGAGCCCAGATCT1260.12507693224006827No Hit
ATAGTTGGGGAAGAACGCAAAGCTGAGAACATGGACGTTAATATCGCCCCAC1260.12507693224006827No Hit
TGCAGTGGGAACCAAAGGGGTGACCAGCCCTCAGATGTGCTTATTGGATTCC1250.12408425817467092No Hit
AAACTTGGGCAGTCTGTTAGTTTTCTTTTTGCTTGTTTATCTATGCAAACAG1250.12408425817467092No Hit
TGCATAGGGATAAGGTATTTATTCAGCCTTTTATAACTCTAGGAGTTGAAGT1250.12408425817467092No Hit
AAACTGAGGGAACTGCCAACGAGATGCTTTAAAAATATTCCCTTTTGTATAG1240.12309158410927355No Hit
CTTAAAGGGATCCAGATTGGGTGGGGAAGAAAAGGCTTTCTGAAAGAATGAG1230.12209891004387619No Hit
TACTTTGGGACACAGGCACATGAAATAAAGGCCATAAAAAGAAAGAAAAGAC1230.12209891004387619No Hit
AGTGACGGGATTTTTGTATGGCTCTAAGTTAAGGAAGCTTTTTATATTTCTA1220.12110623597847883No Hit
CATTTCGGGTATGTGACAAAGGTGGCATAGCCCATCATGAGGAAAAGGGGAG1220.12110623597847883No Hit
GGCTGCGGGTAAAGCCAGAATCCTGCTTTATAGCCAGTGGTATGCTGGTACT1210.12011356191308147No Hit
ATTCCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1210.12011356191308147No Hit
GCATCAGGGAGAACACACCTCCTTAAAAAGCATATACTTGTGATGAATTTTG1200.1191208878476841No Hit
GCAATGGGGGCGCAATTATAGATCTGGATTTTGAACCACTTAATGAAGCGGC1190.11812821378228673No Hit
GTTAAGGGGGACCTTGAAACTAAAGGAAACTAATCAGCCCACTTTCAAAATT1190.11812821378228673No Hit
CTATCCACGGGATTTCATGGTAGGTTTTTAAGCTTTATTGCTTTTATTAGGG1180.11713553971688935No Hit
TAATTTGGGATTGATTATTTGAGGAAAATCTCTGTTTCCTAGATACTTTGTA1180.11713553971688935No Hit
TACGTTGGGCCCGTGATAGATTATTGATAGCAATAGAGAGGGGCGGCTTTGT1180.11713553971688935No Hit
TCGCATGGGGAATCTTCCAATTTTATCTATTTGATTCAAATGGTATGTTTTT1170.11614286565149198No Hit
GGAAGAAATGAAGAGTGGACTGAAAGATTTCAAAACATTTTTGACAAATGAT1170.11614286565149198No Hit
CGCAGAGGGTTGAAAGCAGATTTTCCCCTTAATCCTCAACCAAATAAAATGA1160.11515019158609462No Hit
CACAGCGGGAATTAAATGGTGATTAAAAATTTATCTCCTTTAAATCTAAAAA1150.11415751752069725No Hit
GCGACCGGGAAAGAAAATCAGAGAGTGATAAAGATGTAAAAGTTACACGGGA1150.11415751752069725No Hit
TAGGCTTGGGGGGATCAATGTAGCAAGAAGAGCTAACTATATATATGCACCC1150.11415751752069725No Hit
CATCTTGGGGACCAGCTCCCCGCTGCCCTGCGCTCGGCGCGCTGGCATCCGG1140.1131648434552999No Hit
AAGACGGGCAGAAAAGAATATGAGCTATGTCACTGTTTTGTATGTGGGGAGT1130.11217216938990253No Hit
CAGTGGAAATTGGATCATAAGCCAGTGTCCTAAAGGATGAATAGGAATTAGC1110.11018682125910778No Hit
CGTTAAGGGCTTTTATTTTTCTGCTCATTTCTGAATGAAAGGACAGCTTCTT1110.11018682125910778No Hit
TACTTAGGGATTGTAGCCCGTGAACACATTGACAAGTACAAAAATTAAAAAA1110.11018682125910778No Hit
TACCTGGGGGGCGCATGCTCAGGCGGGGATGTGTGCGAAGCCTGCCGCTGCT1110.11018682125910778No Hit
CGCCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1100.10919414719371041No Hit
CCAAGCGGGTGAGCAGCTAAAGTAATTTCCACTTTTATGATTGCTGGCTAGT1100.10919414719371041No Hit
ATTTGTGGGCAGTCTTTCAAAGCCAGCCAGGCTACACACAGAAAATGTTTAC1100.10919414719371041No Hit
CATCACGGGACTTCCCAATACATCAGAAAAGCTCTTTCTCTTTTTGTGTCAG1100.10919414719371041No Hit
GAGTATGGGGATCAACCTGGAGCTCTACGCCTCCTACGTTTACCTGTCCATG1100.10919414719371041No Hit
GAGGCGGGAGAATGGCGTGAGCCCGGGAGGCGGAGCTTGCGGTGAGCCGAGA1100.10919414719371041No Hit
CTTGCTGGGAGGACAGCCAGAAGCCCAGCCAGCCCCAGACTCCAGTGCACCA1090.10820147312831305No Hit
CTTATGGGGGTAGCCTCCCTGGGCTGATCATTCCCTCTCCTTGTAATTGCAG1090.10820147312831305No Hit
CTCATGGGGACACCAGCATTTTAAAGAAATTTTATTCTATGTTACAGGAAGA1090.10820147312831305No Hit
TAGCCGGGGATCTTCAGGTGACCTGAATCTTTCCCTTAACCGTACAGTTTCT1090.10820147312831305No Hit
AAGTGCGGGAGGATCTTTTTTTGCAAACAACTGATATATGCAGACAAATTTT1090.10820147312831305No Hit
CATCTAGGGGAATGGGAAACAGATTCCAAATACATTCATGAAAAGAGTAACA1090.10820147312831305No Hit
GCACCTGGGGAGGAACACAATCAAGGTGCCACTGGCCATTTCTTGGGTCCCT1090.10820147312831305No Hit
GCATTTGGGGATGATCAAACTTGGTCTGGGTATTGATGAAGATGACCCTACT1090.10820147312831305No Hit
CAGAAGGGGATGCATAGAAGTGGAAAGTAGGGTTTCTCATTTTAAGGTATTT1080.10720879906291568No Hit
TTGCTAGGGACAGGTAACTGTTTAATCCCTTACAATTTTAAATGAGGTATTT1080.10720879906291568No Hit
ACAACCGGGAAAGTTTTTAATGTCAACAAGAAAAATGCATGGATGTTCTTAA1080.10720879906291568No Hit
AGTACTGGGAGGCGTGAGCCACTGCGCTTGGCCTCATGTAATATTTTTGTAT1070.10621612499751831No Hit
GATATAGGGGGTTCCGGGCGCTTGGAGAAGATGGTGCTGCGGCGGCTGCTGG1070.10621612499751831No Hit
CGTACCGGGCAGACTCGATGGAGAGCTTCCCAGAGTCCCTCTCTGCCTTAGT1070.10621612499751831No Hit
AATAGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1070.10621612499751831No Hit
TAGCTCTGGGGGTTACACGAGGTCTGAGAGACAGAGGCAGCGTGTTTGAGCT1060.10522345093212096No Hit
TCTTGCGGGACCCCATTGGTTGTTAGCCAATAGTAGTCACAGTTCAAAGATG1060.10522345093212096No Hit
GGGATGGGAGGTTGAAAGTAAAATGATAGAAAAAGATATACCATGCAAACAG1060.10522345093212096No Hit
ACATGTGGGGCCTAAAGAAATTTTTCAGTTATTTGTCTCAGAAAGTATATCT1060.10522345093212096No Hit
TTACGAGGGGTGGAATTGGGGTATGGGGAAGGCATGATTGCATAAAGATCAT1050.10423077686672359No Hit
GAGGCGGGGTCAGCTTCTCATCCTTTAAAAGATTTTTACTGGTAGTGTCCTC1050.10423077686672359No Hit
TAGTACGGGTCCTGGCCTGAAGTGAACCCTGTTTCTAAAAAACACAAACTAC1040.1032381028013262No Hit
AGTGTTGGGTCTGATATTTTACAAACCTGACAAAATGAGCAATGGGGAAAGG1040.1032381028013262No Hit
AGGATTGGGAGAGGGATTGCAGATGGGAGCTGTCCACATACCGAAGTGCTGA1040.1032381028013262No Hit
TAGCCAAGGGTAGGAGAAAGAGATACGAAGTACTGGCAATACAATTAGAAAA1040.1032381028013262No Hit
TATCACGGGAATTTGCAAAGAGCTTCAAACACCAAAAATATACCATTTTAAG1040.1032381028013262No Hit
GCATCCGGGGTAGACCTGATCAAAATATCACCTAGTTGAAATCACTTTAGTA1040.1032381028013262No Hit
CGACATGGGAGCTCTAACATCTGACCTCATTTCCTGACATCCTGGCCCTTTG1040.1032381028013262No Hit
GCGCGGGGAATGAAACAGAAGCTATCACACATCCCACATACGCTTAAAAGAA1040.1032381028013262No Hit
ATGGATGGGAACCCAAACATCATGTGATTCATCTCTAAATATTTCAATTGTA1040.1032381028013262No Hit
CATCATGGGTGCTCATGGGTAGGAAAATCAATATCATTAAAATGGCCATACT1030.10224542873592884No Hit
ACAAACGGGGATCTGCGTATATCTTTTTACTCTCCCTGAACTTAACTACTAA1030.10224542873592884No Hit
GTTACTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1030.10224542873592884No Hit
ACTACGGGGGGGAGAAAGATGGCGGCTACCTTACTAGCTGCTCGGGGAGCCG1030.10224542873592884No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCCTGTCTCTTATAC1030.10224542873592884No Hit
GACGCAGGGTACAGCAACTGACCAGCATTAATGTCAAAATAAAGATCATAAG1030.10224542873592884No Hit
GGTACAGGGAGTTCAGTATTGCTAATGGAATATATTATTTACACAAGACTTT1030.10224542873592884No Hit
AAGTTAGGGGGGATCTGCTCACTTCTGGTCTTTTTGGCCGGGAACTCCTGAT1030.10224542873592884No Hit
GGTCAGGGCAGACAGAAAGTTTTTAAAAAATGATGATTTAAGCATTGAAAAA1020.10125275467053148No Hit
TCAACTAGGGAAGGAAGGAGTAGGTCGGGAAGTTCTAGTAGTGGTAGTTCCA1020.10125275467053148No Hit
TAATGGGGAGTGACATAAGTAGCAGCCGTTTTTCAACTTCAGTTTCATTCAC1020.10125275467053148No Hit
TTAGCCCGGGCATAGAGATTATATTGTATCAGTGTTTATGTAAGCTGGAATC1020.10125275467053148No Hit
CATCTGAGGGATAAAAATAAATGTTTTAATTTACTTAGAGCTGTATTCAAGT1020.10125275467053148No Hit
ACCACTGGGATAGCTGAAGTCATCATGTAGCTGGGATATTCTATAAACATTT1020.10125275467053148No Hit
CTAGTAGGGCATTTTTCCTTGTATTTTTAGAGCGAGGCTTCCTTCATCTCCT1020.10125275467053148No Hit
CGTGGGGGGATCACCAGCTGACCACTAAGATAACAGAACAGAGACTTCCATG1020.10125275467053148No Hit
CGAAATGGGCAGGGAGGGACCCACCAGAGCACAAGAGAAGGTGGCTACCTGG1020.10125275467053148No Hit
TACTTAGGGGATCTGCGAGCGGGGACTGTGGAACTATTTTACCTTAAGACCT1020.10125275467053148No Hit
CTACTTAGGGGATGATATGGAATGGCCAACTTATTCTAGAAAATTTACATTT1020.10125275467053148No Hit
TATGGTGGGACACATAATATATAGTAAATGGAAATGTTTAAAAGCAACTTTC1010.10026008060513411No Hit
GAGCTTGGGCCACTGGACACTGTTTGAAAGTTTGTAGTTTATAATATTCTTA1010.10026008060513411No Hit
ATTAGTGGGCTCCACCTAAGCGAGCAGAGATGAAGACTTCCATGGAGTCACT1010.10026008060513411No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)