FastQCFastQC Report
Sat 31 Dec 2022
SRR5849170.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849170.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124340
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGC8350.6715457616213608No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGCCGTCT8300.6675245295158436RNA PCR Primer, Index 47 (95% over 22bp)
AAACGAGGGAATGGTGGTAGCAGCTGAGGTATGGTTGTTGGACGAGGCCGAT2110.1696959948528229No Hit
CACGTTGGGCTAGCGGAGGGAACTTGAAATCAGGTAGTGTTAGCAATCCAAC2070.1664790091684092No Hit
TAAACTTGGGATAGCATGCAACATAAATGAAGTTATAGATCCCCTGGCTGTT1990.1600450377995818No Hit
CTTTGGGGTATACTTGGTCCACCGGGTTCTGTATATGAAGGTGGTGTGTTTT1980.15924079137847835No Hit
ACTTGCGGGAACAATATTGAATTGAGAAGCTTGTAAAGTAGGCAGAAGTAAA1970.15843654495737494No Hit
GGTCCTGGGATGGAAATTCAGTGGACGAGTAGTAACTGACTCAGCGGAATCA1970.15843654495737494No Hit
ATGGTAGGGAGGGGAACAGGTTAATTTATTGAAAGAAACAATTAAATATATG1950.1568280521151681No Hit
TGGACCGGCGGGATCTCAGTAAATACATAAATAAAGAGAAATGGCAACAGCA1920.1544153128518578No Hit
GGTCAGGAAAGTGATTGAATAAAAACGCTGAGGTGGATTGGCTGCTAGAGGC1910.1536110664307544No Hit
CGTAGGGGGCGGACTGCGGACTGCAGTGGCGCAATCTCGGCTCACTGCAAGC1880.1511983271674441No Hit
GATGTTGGGATCACCATGGGGATGGTTCTTCTAAATGAAGCAGCAACTTCGA1870.15039408074634067No Hit
CTCGATGGGGACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTCGGC1790.14396010937751327No Hit
TTAAGTGGGACCTCTATAAAGCTGGGAAGGGGAATGTAGGTACCAGCTACAC1780.14315586295640984No Hit
ATCACGGGCATAAAATATTTAAGTATTTGAAAAAGAAACAAAATGTCTTCAT1780.14315586295640984No Hit
ATTTGAGGGGATGGGCACAAATGGAAGGTGCGCACTTGCCCCTACTATTGCC1770.14235161653530642No Hit
TAACGAGGGGACAAATAATGATGACATTATTTGTCATCTTTGTTTGTGAGAT1760.14154737011420299No Hit
TTGAATGGGCCGGTTGGAAACGCCAAGCCAGCTGCGTCCTAATCCAAAAGCC1760.14154737011420299No Hit
TTTGTAGGGTTACCCAGCACCCCAGGTTTCCACAGCTGTAGTTGAGCCCTAC1760.14154737011420299No Hit
CACTCTAGGGGAATGAGGAAAAATATTTACAAACTATGTATCTGACAAAGAT1750.14074312369309958No Hit
ATTCGCGGGTAAACCAAAATTAGCAATGAATCTGGCAGGCTTTTTCCAAAAG1740.13993887727199614No Hit
TAGTGGGGGACCTGTTTGTCAGCCACATCACGCTGTTGACTTGTGTTAAGCT1710.13752613800868588No Hit
ATGCTAGGGGGTAGGAGGAAACCTTGTTATCAACAACCCTGACAAACAGAAA1710.13752613800868588No Hit
TATTACGGGCACCAATGGGGCTTCTACTAGGTGGCGTCAAGTCAACATATAT1700.13672189158758244No Hit
CACAGTGGGGCTCAGAGGAAGAAATTAGAATGAAAGTCATATCATACCTATC1700.13672189158758244No Hit
CCAAAAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1680.1351133987453756No Hit
TGGAATGGGCAGAAGAATGGGCCTTTCTGCCATAATTCTTCACTAGTCAAGA1640.13189641306096186No Hit
TCAATAGGGCACAAACAGAACAATACATTCAAAGAAATCTCATTTGCATAAC1640.13189641306096186No Hit
TAGTTCGGGTCCGTACATTGCTATTTTGCCACACTGCAACACCTTACAGATG1620.13028792021875502No Hit
GTCAGGGGGATCAAAAAGATATGGCAATTATAAACACACATCTAATAACAGA1610.1294836737976516No Hit
CTGGTTGGGGGCAGTGGTGCTGGGAGTGTCGTGGACGCCGTGCCGTTACTCG1610.1294836737976516No Hit
CAAGACGGAAAGGAGAAAAAAAATACAGAAATTGCAACTGGATCTGACATTT1610.1294836737976516No Hit
CGCGACGGGATAATCAGAAAAGAAAATCCAGTTAAATTTATGAAGTGAGATT1610.1294836737976516No Hit
CAGTGGGGATATTAGATTTACTGCCGTATCCCTCCATCTTACCTAAATGAGA1580.12707093453434132No Hit
CTCACCGGGATTCTCTCTCATGATTCTTGGAGGCTGGAAAGTCCAAGATCAA1580.12707093453434132No Hit
GCTGTGGGGTACCATAAGCAATCCCTTGCCTTAACTCATCACCCTTTTTCAC1570.1262666881132379No Hit
GGCTTAGGGCTAAGAGAACAGCCAGATGGTTTATATGAATAATCTTTATCTG1570.1262666881132379No Hit
TTGCGTGGGGCCTGTTAGACTGGTGAGAGAACTCTAGGTACTTTGTTGGGAA1570.1262666881132379No Hit
GATACTGGGGAATTCGAATGGACAGTTACACCAGTTACTGCAATGCTGTGTC1560.12546244169213447No Hit
GGTATTGGGAAGGCATATACAAAATAAACAGAAAAATGACACAAGACCCCTT1540.12385394884992763No Hit
GACAACGGGAGTCTGCAGCCGCGACGTCTGATGTTAGGGACTAGAACTGAGA1530.12304970242882418No Hit
TCTCTAGGGAAAATTACAAATTGAAAGTCAGGGAAGACTTATCTAAGCAAAG1520.12224545600772077No Hit
TGTAGGGGGATATTGGTGAAATGGGAAAAGACTACATTTATGCCGTAACACC1520.12224545600772077No Hit
ATTGCCGGGAGCTGAACAAGTTGGCCTTCCTGAACCAGTTCCACTTTGGTGT1520.12224545600772077No Hit
TTGAGGAGGGCTGACTATATGGAATAAAACTAGAAACCAACAGTAGATGAAC1520.12224545600772077No Hit
TTACCCGGGGGAAAAAGAGGAGTCCAAGACAGCTTCTGAAGTTTTTGGTCTA1510.12144120958661733No Hit
AACTCTGGGGATAATAAAAAGAAAGAAATTAAGTCTCGATGTAAATCATTGT1500.12063696316551391No Hit
CGTATTGGGAGGACTCAGAGGCCGCCATCAACCGCCAGATCAACCTGGAGCT1500.12063696316551391No Hit
GATAATGGGTGGCTGAGACTGCTAAGAGTGAACCTAAACACTTACAAGTTGC1490.1198327167444105No Hit
AAGCTGGGTCACCAAAATGTCTCCATTTGAATTGTATTGATAATGTGGGACA1490.1198327167444105No Hit
ATATCAGGGGTACCTGCAGGCCTCCTACACCTACCTCTCTCTGGGCTTCTAT1490.1198327167444105No Hit
GACATTGGGCTGAGTGAAATATTTGCTTTCATTCTTTCCCTTTGCCCTCTTG1480.11902847032330705No Hit
TGTGATGGGGAGTAGGAATATGAAAATGACACCTTTTCCAAATATTAAATTG1480.11902847032330705No Hit
ATGTAGGGGAATTTAGAATAACAACGCATCCAGGACCCTTTGGGTTACTTAA1470.11822422390220363No Hit
CGCTAAGGGGGTATGTGTGGTTGGGGAATTCATGTGGAGGTCAGAGTGGAAG1470.11822422390220363No Hit
CACGCCTGGCCCGGTCCCTTCCAGGGCAACTCCCAGGGATGTGGTGACATGC1460.11741997748110021No Hit
CTGCCAGGGGATTATGGAGAATTGGGGCACCCACAGGAATGAAGAGAGAAAG1450.11661573105999677No Hit
ATACTGGGGGGCCCGAAGCCCGGGGACGAACCGACGGACCGACCGCCTGGCG1450.11661573105999677No Hit
TGCCAGGGGTTATCCAGCTAGACAATATTTTATGCATATTTACCGTGATGTC1440.11581148463889336No Hit
GCAGGTAGTCGATTGAGTCCACCAAAATCTTAAGCTAAATTTTATGTTGTTT1430.11500723821778994No Hit
TGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG1430.11500723821778994No Hit
CTCGTTGGGGTGACTAGCAGAGTCAAGGGTTCAATAATCGTTGAATGAATTA1430.11500723821778994No Hit
CAGGATGGGCTTGGGGAATGTTAACTGAAAAAGCTCAAATGTCATCTCTTTT1430.11500723821778994No Hit
TATTTAGGGTGACCGACCAACACAGTACCAAGAGGATGAAGAGAAGATGTTT1430.11500723821778994No Hit
AAGCCTGGGCGACAGAGCGAGACTCAATCTTGAAACCTGGATTGAGAATGGA1420.1142029917966865No Hit
CAGAAAATAATTAAGAAGAATTTATGTCGAAACTTCATGCTTCATCTAGTCA1420.1142029917966865No Hit
ATTCAGGGGGCGGCGCCTACCGCCCTGGCCGTGTCTGTCTGGTGTGTGCTGT1420.1142029917966865No Hit
AGCACTGGGAGGCTTAAATGAGGTAGCTTTTGATTCAAAATGGAAAAATAAT1410.11339874537558307No Hit
CTTCCAAGGGAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATA1410.11339874537558307No Hit
CTCTCACGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGG1410.11339874537558307No Hit
TTACGCGGGGAGATACTGCGAGTATGGCGGCGTCAAAGGTGAAGCAGGACAT1410.11339874537558307No Hit
TGGAAAGGGATCACAAAGGCAGCCCTCAAATTATGCAAGCTTGGAAATCACT1410.11339874537558307No Hit
CTGTCGGGGTACTCTTAACCTCCCAAAAAGCAGTATCTAAGTCACATACATG1400.11259449895447966No Hit
AACAGTGGGATGTTTTAAAAATAATATTCTAGCTATTATGCAGAGGATGGAT1400.11259449895447966No Hit
ATATGGGGGAAATTTCTTGCAGAATCAGCTACACTTAATTATGTTGCTGATA1400.11259449895447966No Hit
ACACAGGGGGAAACCTGGTAGTCTGATAATAGTCTGAAACCTGATAATGGTA1390.11179025253337622No Hit
GAGTGAGGGGATCTTGGTACGGGTACACTCCAGATTGAAGAGCCCAAGGTGA1380.1109860061122728No Hit
CAACTAGGGCTTCAGGAAACAGAAAAAAAGAGCACAGTTACCTAAAGTAAGC1380.1109860061122728No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCCTGTCTCTTATAC1380.1109860061122728No Hit
ACATCCGGGTACAGGCATGAGCCACCATGCCCAGCTATGGAATTTTTTATGT1380.1109860061122728No Hit
AACGCGGGGATATTTTAACAATTCACAACATACATTTTGGTGTTTCTGAAAG1370.11018175969116939No Hit
TCACTAGGGTCTTCCTGCTCATGATGCTTGGTGTTCGTGGCCTCCTCCTGGT1370.11018175969116939No Hit
TTTACTGGGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCT1370.11018175969116939No Hit
GTCAAACGGGTCTTCTATCGGGGGGGCTGACTTGTGTTGTGATTATTCCAAT1370.11018175969116939No Hit
GGCTTTGGGGGCAGCCCGAAATCATCCTGCCCTCCAAGGCACCGCCCTGATG1370.11018175969116939No Hit
AATTTGGGGAAGAAAGCTCCATTTTTATCATAAACATTTCAACATAACCAAC1370.11018175969116939No Hit
GATTGAGGGTATTCTGAGACAATAGGCAAATGAATTTTTATATAAAGAAAGT1360.10937751327006595No Hit
ACTAGTGGGCAGAGCCAGAAAGGTCTGAGAAGTGGACTGCATAGTGCAGACC1360.10937751327006595No Hit
TATCTACGGGAAATTATGGCATTTAGATCCAGATACCGAATATGAGATCCGA1360.10937751327006595No Hit
GTAATAGGGATGAGAATTGACTGCCAACTAAACCAACTGTTAAGTTATCTAG1360.10937751327006595No Hit
ACACTAGGGGTAGTGGTCTGTGGCTACAGGAAGCTGGTTCTGTCTGCTTCCA1360.10937751327006595No Hit
GACCGGGATCAATCTTGAGCATGAAACCACCTGAGGCCTTAGCCATGTCTCT1350.10857326684896253No Hit
CTCATCTCGGGAAGCAGGGAGAATAAAGATAATTGTATTTTATGTTTTGCAC1350.10857326684896253No Hit
AGCCACGGGGTTCCTACACTTTTGATGTCAAAGCCACTGATGGAGGACAACC1350.10857326684896253No Hit
GCTGATGGGAGTTCAGATCTGAGTCTGAATCAAGGACACTTGAATCTGACTG1350.10857326684896253No Hit
GCGCCAGGGAGAGACGCCAGAGGTGCAGCTCCAGCAGCAATGGCAGTGACGG1350.10857326684896253No Hit
TTAGCTGGGTCTATGCGAGGGGTGATGTGAAAAATCAATTTATTATCCTTGG1340.10776902042785909No Hit
GAGATGGGGCTTGGGAGGTGAGGCTTCCCAACCACGGAAGACTACTTTAAAT1340.10776902042785909No Hit
TCATTTGGGGACTGTGAAATATATTTGAAATGATGAAAGAAAAATGTCAGCT1340.10776902042785909No Hit
GGTTCTGGGTGAGAAATCCAAGGAAAGTCAAGTGACTGCTGATGACCTTGAA1340.10776902042785909No Hit
CTCTAAGGGAACTATATACTTTCTACAATAAATGTATTTCAAATGTTATTAT1340.10776902042785909No Hit
GTTGGCGGGATAGATTGGAAGACTTCTAATTTTAAGATGACAGTGCTCTCCA1340.10776902042785909No Hit
ACATTGGGCAAACTAACAAACAAGAAACAGAACAAATAATAATTAAAAAGCT1340.10776902042785909No Hit
AGTAGCGGGAGTCCACCAGCCAAGCAGCATACTTGGGTTGGTACTTTCTCTC1330.10696477400675566No Hit
AATGAGGGGACACACATTAAATCTTAAAATTTTCCTGAGGTTTCTGGATATA1330.10696477400675566No Hit
TGTAAAGGGCACACAAACTCTTAAAAACGAGTTATTGTCTGACATTTTGTTT1330.10696477400675566No Hit
ATACTTGGGTGTCCCATCAAAATGGGCCACGTAGCATCTACTTACATCTTTG1330.10696477400675566No Hit
GAACTCGGGGGGAAGAGAACAAGAGTCTTTGCCTTGTAATCCAAAGAATTAT1330.10696477400675566No Hit
CTGCATGGGATGCATCATTCTCTAGAGTTGAAAGGATTGACATTTCTCAGTA1330.10696477400675566No Hit
GAGTTGGGGGCCACTTCTTAAAACAGAACAAAACAAAAAAAGAAAATTGTGC1330.10696477400675566No Hit
GAGGCAGGAGAATCGCTTACACCTGGGAGTGGAGGAGCCAAGATCATGCCAT1330.10696477400675566No Hit
TTCTAGGGATAGTTGGAAAATAATAGAATGGCTTTAGTAGAAAAATAAATCT1320.10616052758565225No Hit
TAGATTGGGGAGAGCCATTCTAATTGTGACTCAGTATCCAGATACCCCAAAT1320.10616052758565225No Hit
AACTATGGGGCTCTCTCCCGGGGGAGCAAAATGGACGAAAGGCGACCCCCCA1310.10535628116454882No Hit
ATTACTGGGGACTTATAGGTGATAGTGATAATTCCTGATTCCAAGAATGCCA1310.10535628116454882No Hit
AAAAAGGGGGATATGCCAGACACTATGAGAATACATTGAAAGGTTTTTATCT1310.10535628116454882No Hit
TAGAGCGGGAAGCTCGCTGCGATGCTGAGGCTGGACGGGGAGATGATTATGA1310.10535628116454882No Hit
AGACCAGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1310.10535628116454882No Hit
CTTTCCGGGTGGCTATAATAAGATGTATCACAAACCACCCTTGACTAGAATG1310.10535628116454882No Hit
TACATAGGGATTGGAAAAAAGACAACATTGATGTTATTTGTGCTCTTTGTTT1310.10535628116454882No Hit
CACGTTGGGATCTGAAGTTGGGCAAGACAATTTAGCTTGGAAAAATAATAGT1300.10455203474344539No Hit
TACAGTGGGGGAGTGATGGGGATTTAGAAGAAAAGGATGGCTTTCTGCCAGA1300.10455203474344539No Hit
ACTCGTGGGACAACAACAAAAATCACTTGCAAATGAAGTGTTTTAGTAAGTA1300.10455203474344539No Hit
CTCCTGGGGGCGCCGCACCGCACGCAGCCGAGCAGCGCCACAGCCGCCGCCG1300.10455203474344539No Hit
TTATATGGGAAGTTGCATGTGCTTGAAATAGATTTAATTCTTATTCCCCACA1290.10374778832234197No Hit
TGTCAAGGGAGAGAGGATCTCGGTCACTTGAATGAGCGGCGGCACTGCAGCC1290.10374778832234197No Hit
TTGCAGGGAGACTTGAACATCTATGGATTTTGGTATTTGCAAGGGGTGAATG1290.10374778832234197No Hit
ACTATTGGGGCACAAGGCAATTGCCTGATGTAAAAGAGATAAAGAAATGTTA1290.10374778832234197No Hit
GTATTGGGGGATCTGGTGCTAAGCCAGTGATGTCCAACAGAAATGCAATGCG1290.10374778832234197No Hit
GCGCGAAGGGCAAACCCTTAAGTTATGTACAATTATATGAGGAACTAGGTCT1280.10294354190123853No Hit
AGCTTGGGGGAGAGAAAGGTAAAAAGGAAAAACTTTTGAGAGAGTAAAATGT1280.10294354190123853No Hit
TTGTTAGGGGATCTCGAGAAGGGGGAAGTGACAGGCTCAAAGCAATTGGGGC1280.10294354190123853No Hit
CGATTAGGGCACTGCGAAAATTCATTCCGCTGTAATGTTTTCACAATATTTA1280.10294354190123853No Hit
TGTTTGGGGAAAGTGTTGGGATTGTAAGCATAGGCCACTGTGCCCAGCAAGA1280.10294354190123853No Hit
GCCGTTTTCTATTTCTCCCTGGGCTGCGGAAAGCCAGAAGATTTTATCTAGC1270.10213929548013512No Hit
TCATTAGGGAGGGTAATGGAAGATGGAAAGAAGAGAAATCGTTTTATGGGTT1270.10213929548013512No Hit
GGTAGGGGGAAGCTCATCGATTCCATATGCTGTCACCCAGGGTGCAGATTTA1270.10213929548013512No Hit
TTATGCGGGTTGTATGCATTTGAGAGAAATAAATATACCCATACTTATGTTT1270.10213929548013512No Hit
TTCACCGGGAATAACCAAATGCTAAAAGAACTGGCATAGAAGTAAATGGGCT1260.10133504905903169No Hit
CACTAGGGGATCTTGCTCAGGGATAATGACCCAGGAATAGCTAATGACACAG1260.10133504905903169No Hit
TACACCAGGGCTATATTGATTTAAAAGTGTATTTTATTCAGTAGTTTGAGGA1260.10133504905903169No Hit
ACTTGAGGGACCAGGAGAACATATAAAAATGCTGTTCTCCCCATAGGTTCTC1260.10133504905903169No Hit
AACTCAGGGGGTACCTAGTGACTTCCAACCAAAGCCTTTGAGTATGCACTAA1260.10133504905903169No Hit
AAGCATGGGCCACCTATCTATTCACATGGACCAGCAAACGGGACTTCTCTCT1260.10133504905903169No Hit
GTTTTGGGGGTCTGGGAAACCACTCTGTCTTGAAGATTTTTTTCCCCAAAGG1260.10133504905903169No Hit
AGTTGAGGGATCTGGATCATTAAAGTATGTCCTTGTTTAGAAACTTTGGCAG1250.10053080263792825No Hit
TGCCTGGGGCTGAAGATGCAGCATTTCCTTTCAGCCTAATGTTGACCTTAGC1250.10053080263792825No Hit
TATAGTGGGATCCTTAGAAATTATAGTTTTTAATATCTTAATATGCATCTCT1250.10053080263792825No Hit
TGCATCGGGAGATGTGTGCCAGAATATTGTAAATTTTAACATAACTTTTACT1250.10053080263792825No Hit
TTCTTGGGGACAACATTTCGGTGAAGAGCTGGAAACTTAAAAAATTATCAAA1250.10053080263792825No Hit
GTCTTTGGGACCAACCCAATGAGTATCTGAAAGCTTTGGTAGCTGTGGGGGA1250.10053080263792825No Hit
TGCTGTGGGGAATATAACAATGTGTTTTTAAGTAATCAATTCATTTAAAAAA1250.10053080263792825No Hit
AACAGCGGGAGGCGTGAAATGTGCTATCTTGGGTCATGAATTTGTCAGGGGC1250.10053080263792825No Hit
GTACGCGGGAATGTAAATTGTTTTTTGTTTTTTGTTTTTTGTCTGGTTTAAA1250.10053080263792825No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)