FastQCFastQC Report
Sat 31 Dec 2022
SRR5849169.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849169.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123722
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGC8610.6959150353211232No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGCCGTCT8060.6514605324841176RNA PCR Primer, Index 47 (95% over 22bp)
GGTCCTGGGATGGAAATTCAGTGGACGAGTAGTAACTGACTCAGCGGAATCA2120.17135190184445773No Hit
ACTTGCGGGAACAATATTGAATTGAGAAGCTTGTAAAGTAGGCAGAAGTAAA2040.1648857923408933No Hit
AAACGAGGGAATGGTGGTAGCAGCTGAGGTATGGTTGTTGGACGAGGCCGAT1980.16003621021321995No Hit
GATGTTGGGATCACCATGGGGATGGTTCTTCTAAATGAAGCAGCAACTTCGA1950.15761141914938329No Hit
ATTTGAGGGGATGGGCACAAATGGAAGGTGCGCACTTGCCCCTACTATTGCC1930.15599489177349218No Hit
CACGTTGGGCTAGCGGAGGGAACTTGAAATCAGGTAGTGTTAGCAATCCAAC1920.15518662808554662No Hit
CGTAGGGGGCGGACTGCGGACTGCAGTGGCGCAATCTCGGCTCACTGCAAGC1880.1519535733337644No Hit
TTGAATGGGCCGGTTGGAAACGCCAAGCCAGCTGCGTCCTAATCCAAAAGCC1850.14952878226992775No Hit
TAAACTTGGGATAGCATGCAACATAAATGAAGTTATAGATCCCCTGGCTGTT1820.14710399120609108No Hit
TATTACGGGCACCAATGGGGCTTCTACTAGGTGGCGTCAAGTCAACATATAT1800.14548746383019998No Hit
CTCGATGGGGACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTCGGC1770.1430626727663633No Hit
ATTCGCGGGTAAACCAAAATTAGCAATGAATCTGGCAGGCTTTTTCCAAAAG1770.1430626727663633No Hit
ATGGTAGGGAGGGGAACAGGTTAATTTATTGAAAGAAACAATTAAATATATG1760.14225440907841774No Hit
CTTTGGGGTATACTTGGTCCACCGGGTTCTGTATATGAAGGTGGTGTGTTTT1760.14225440907841774No Hit
ATGCTAGGGGGTAGGAGGAAACCTTGTTATCAACAACCCTGACAAACAGAAA1720.13902135432663554No Hit
CACAGTGGGGCTCAGAGGAAGAAATTAGAATGAAAGTCATATCATACCTATC1700.1374048269507444No Hit
TTACCCGGGGGAAAAAGAGGAGTCCAAGACAGCTTCTGAAGTTTTTGGTCTA1700.1374048269507444No Hit
TTTGTAGGGTTACCCAGCACCCCAGGTTTCCACAGCTGTAGTTGAGCCCTAC1690.13659656326279887No Hit
CACTAGGGGATCTTGCTCAGGGATAATGACCCAGGAATAGCTAATGACACAG1680.1357882995748533No Hit
TGTGATGGGGAGTAGGAATATGAAAATGACACCTTTTCCAAATATTAAATTG1680.1357882995748533No Hit
AGTAACGGGAATTCTGTTCTGAATACAGCCACCAAAATGAAAGATGTACAAA1680.1357882995748533No Hit
CGCTAAGGGGGTATGTGTGGTTGGGGAATTCATGTGGAGGTCAGAGTGGAAG1670.13498003588690774No Hit
GATAATGGGTGGCTGAGACTGCTAAGAGTGAACCTAAACACTTACAAGTTGC1670.13498003588690774No Hit
TAACGAGGGGACAAATAATGATGACATTATTTGTCATCTTTGTTTGTGAGAT1660.13417177219896217No Hit
TGGACCGGCGGGATCTCAGTAAATACATAAATAAAGAGAAATGGCAACAGCA1660.13417177219896217No Hit
CTGTGCTGGGAGTGTGAATCTTATTCTGATGATTTTTTGTCCTTAGCATAGT1650.13336350851101664No Hit
TGCATCGGGAGATGTGTGCCAGAATATTGTAAATTTTAACATAACTTTTACT1650.13336350851101664No Hit
TCTCTAGGGAAAATTACAAATTGAAAGTCAGGGAAGACTTATCTAAGCAAAG1640.13255524482307107No Hit
CAGTGGGGATATTAGATTTACTGCCGTATCCCTCCATCTTACCTAAATGAGA1630.1317469811351255No Hit
GACATTGGGCTGAGTGAAATATTTGCTTTCATTCTTTCCCTTTGCCCTCTTG1630.1317469811351255No Hit
CACTCTAGGGGAATGAGGAAAAATATTTACAAACTATGTATCTGACAAAGAT1620.13093871744717997No Hit
GGTCAGGAAAGTGATTGAATAAAAACGCTGAGGTGGATTGGCTGCTAGAGGC1610.13013045375923443No Hit
ATTCAGGGGGCGGCGCCTACCGCCCTGGCCGTGTCTGTCTGGTGTGTGCTGT1610.13013045375923443No Hit
TGGAATGGGCAGAAGAATGGGCCTTTCTGCCATAATTCTTCACTAGTCAAGA1600.12932219007128887No Hit
ATACTGGGGGGCCCGAAGCCCGGGGACGAACCGACGGACCGACCGCCTGGCG1590.1285139263833433No Hit
TGCCAGGGGTTATCCAGCTAGACAATATTTTATGCATATTTACCGTGATGTC1590.1285139263833433No Hit
GACAACGGGAGTCTGCAGCCGCGACGTCTGATGTTAGGGACTAGAACTGAGA1590.1285139263833433No Hit
AGCCACGGGGTTCCTACACTTTTGATGTCAAAGCCACTGATGGAGGACAACC1580.12770566269539774No Hit
TTAAGTGGGACCTCTATAAAGCTGGGAAGGGGAATGTAGGTACCAGCTACAC1570.1268973990074522No Hit
TCTAAAGGGGAAGGGAGGATACCTATCAAAATGCCAAAGCTGCAGGGACACG1560.12608913531950663No Hit
GATACTGGGGAATTCGAATGGACAGTTACACCAGTTACTGCAATGCTGTGTC1560.12608913531950663No Hit
GGCTTAGGGCTAAGAGAACAGCCAGATGGTTTATATGAATAATCTTTATCTG1550.12528087163156107No Hit
CAGAAAATAATTAAGAAGAATTTATGTCGAAACTTCATGCTTCATCTAGTCA1540.12447260794361552No Hit
CTTCCAAGGGAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATA1540.12447260794361552No Hit
TATAGTGGGATCCTTAGAAATTATAGTTTTTAATATCTTAATATGCATCTCT1540.12447260794361552No Hit
ATATCAGGGGTACCTGCAGGCCTCCTACACCTACCTCTCTCTGGGCTTCTAT1540.12447260794361552No Hit
GACCGGGATCAATCTTGAGCATGAAACCACCTGAGGCCTTAGCCATGTCTCT1530.12366434425566998No Hit
AAGCATGGGCCACCTATCTATTCACATGGACCAGCAAACGGGACTTCTCTCT1530.12366434425566998No Hit
GAACTCGGGGGGAAGAGAACAAGAGTCTTTGCCTTGTAATCCAAAGAATTAT1530.12366434425566998No Hit
GAGATGGGGCTTGGGAGGTGAGGCTTCCCAACCACGGAAGACTACTTTAAAT1510.12204781687977885No Hit
TGTAGGGGGATATTGGTGAAATGGGAAAAGACTACATTTATGCCGTAACACC1510.12204781687977885No Hit
TTTGTGGGGTCAATGTGTTAAGTCATTTATGATCATTGTTTCTGATGTTCAG1510.12204781687977885No Hit
GCTGGCAGGGCCTTAAATTTAGTTTAATGATTGTAATGGGTGCTGCATTTGC1500.1212395531918333No Hit
TTGTTAGGGGATCTCGAGAAGGGGGAAGTGACAGGCTCAAAGCAATTGGGGC1500.1212395531918333No Hit
CGTACAGGGTAAGGTAAGATTATATGATTTTGAGGGAGCTTCATTGTAAATT1490.12043128950388776No Hit
CTCACCGGGATTCTCTCTCATGATTCTTGGAGGCTGGAAAGTCCAAGATCAA1490.12043128950388776No Hit
ATCACGGGCATAAAATATTTAAGTATTTGAAAAAGAAACAAAATGTCTTCAT1490.12043128950388776No Hit
GCGCGAAGGGCAAACCCTTAAGTTATGTACAATTATATGAGGAACTAGGTCT1480.1196230258159422No Hit
TTTACTGGGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCT1480.1196230258159422No Hit
TTCACCGGGAATAACCAAATGCTAAAAGAACTGGCATAGAAGTAAATGGGCT1470.11881476212799663No Hit
TCAATAGGGCACAAACAGAACAATACATTCAAAGAAATCTCATTTGCATAAC1470.11881476212799663No Hit
GGTATTGGGAAGGCATATACAAAATAAACAGAAAAATGACACAAGACCCCTT1470.11881476212799663No Hit
CTCGTTGGGGTGACTAGCAGAGTCAAGGGTTCAATAATCGTTGAATGAATTA1460.11800649844005108No Hit
TATTTAGGGTGACCGACCAACACAGTACCAAGAGGATGAAGAGAAGATGTTT1460.11800649844005108No Hit
CGATTAGGGCACTGCGAAAATTCATTCCGCTGTAATGTTTTCACAATATTTA1460.11800649844005108No Hit
TATCTACGGGAAATTATGGCATTTAGATCCAGATACCGAATATGAGATCCGA1450.11719823475210553No Hit
GTCTTTGGGACCAACCCAATGAGTATCTGAAAGCTTTGGTAGCTGTGGGGGA1450.11719823475210553No Hit
CGCGACGGGATAATCAGAAAAGAAAATCCAGTTAAATTTATGAAGTGAGATT1450.11719823475210553No Hit
ACACTAGGGGTAGTGGTCTGTGGCTACAGGAAGCTGGTTCTGTCTGCTTCCA1450.11719823475210553No Hit
TTATATGGGAAGTTGCATGTGCTTGAAATAGATTTAATTCTTATTCCCCACA1440.11638997106415998No Hit
CTGGTTGGGGGCAGTGGTGCTGGGAGTGTCGTGGACGCCGTGCCGTTACTCG1440.11638997106415998No Hit
AGCTTGGGGGAGAGAAAGGTAAAAAGGAAAAACTTTTGAGAGAGTAAAATGT1440.11638997106415998No Hit
TTAGCTGGGTCTATGCGAGGGGTGATGTGAAAAATCAATTTATTATCCTTGG1430.11558170737621441No Hit
ATGTAGGGGAATTTAGAATAACAACGCATCCAGGACCCTTTGGGTTACTTAA1430.11558170737621441No Hit
TGATGCGGGTGTGGCTCTCGGGCCTAAACTATTATAACATTTAAAAACCAAA1420.11477344368826885No Hit
TAAGTGGGTCATAAAGACTAGTTCTTATCAACCTTGAATGACTACAGATTAT1420.11477344368826885No Hit
TTGCAGGGAGACTTGAACATCTATGGATTTTGGTATTTGCAAGGGGTGAATG1410.11396518000032331No Hit
AGCACTGGGAGGCTTAAATGAGGTAGCTTTTGATTCAAAATGGAAAAATAAT1410.11396518000032331No Hit
AAAAAGGGGGATATGCCAGACACTATGAGAATACATTGAAAGGTTTTTATCT1410.11396518000032331No Hit
TCATTTGGGGACTGTGAAATATATTTGAAATGATGAAAGAAAAATGTCAGCT1410.11396518000032331No Hit
CTCTCACGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGG1410.11396518000032331No Hit
CAATCAGGGAGAGGAGGGATGAACAGCAGCTTATGTTAAGACTCAGCGAAAA1410.11396518000032331No Hit
TAGTGGGGGACCTGTTTGTCAGCCACATCACGCTGTTGACTTGTGTTAAGCT1410.11396518000032331No Hit
AAGCTGGGCTCTCTTTAAATCTGTTCAGTTAGTCTCATGTATTCTCAGGGGA1410.11396518000032331No Hit
TGTTTGGGGAAAGTGTTGGGATTGTAAGCATAGGCCACTGTGCCCAGCAAGA1410.11396518000032331No Hit
AAGCCTGGGCGACAGAGCGAGACTCAATCTTGAAACCTGGATTGAGAATGGA1400.11315691631237776No Hit
ACTTATGGGATAGGTGTGAGCCACCGTGCCGGGCCTGGACTTTGTACTTTTT1400.11315691631237776No Hit
CTCTAAGGGAACTATATACTTTCTACAATAAATGTATTTCAAATGTTATTAT1400.11315691631237776No Hit
GAGTGAGGGGATCTTGGTACGGGTACACTCCAGATTGAAGAGCCCAAGGTGA1390.1123486526244322No Hit
GCCGTTTTCTATTTCTCCCTGGGCTGCGGAAAGCCAGAAGATTTTATCTAGC1390.1123486526244322No Hit
ATTACTGGGGACTTATAGGTGATAGTGATAATTCCTGATTCCAAGAATGCCA1390.1123486526244322No Hit
ACTAGTGGGCAGAGCCAGAAAGGTCTGAGAAGTGGACTGCATAGTGCAGACC1390.1123486526244322No Hit
AGATAGGGGAATTAGCATTTCTCTGAGGATTAGTGATGTTGAACATCTTTTT1390.1123486526244322No Hit
GCTGATGGGAGTTCAGATCTGAGTCTGAATCAAGGACACTTGAATCTGACTG1390.1123486526244322No Hit
TTCGTAGGGGGGGAGCAGAGGGAGATACAGAAACCGACAGGGGCCAGGCGCC1390.1123486526244322No Hit
AGTAGCGGGAGTCCACCAGCCAAGCAGCATACTTGGGTTGGTACTTTCTCTC1380.11154038893648663No Hit
TAGAGCGGGAAGCTCGCTGCGATGCTGAGGCTGGACGGGGAGATGATTATGA1380.11154038893648663No Hit
CTGCCAGGGGATTATGGAGAATTGGGGCACCCACAGGAATGAAGAGAGAAAG1380.11154038893648663No Hit
ATTGCCGGGAGCTGAACAAGTTGGCCTTCCTGAACCAGTTCCACTTTGGTGT1380.11154038893648663No Hit
CAGGATGGGCTTGGGGAATGTTAACTGAAAAAGCTCAAATGTCATCTCTTTT1380.11154038893648663No Hit
AGCTTTGGGGCAAAACAAAAATCCAGTTTAACATGAACAAAAGATTTGAGCA1380.11154038893648663No Hit
GAGGCAGGAGAATCGCTTACACCTGGGAGTGGAGGAGCCAAGATCATGCCAT1380.11154038893648663No Hit
TGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG1360.10992386156059553No Hit
AACAGCGGGAGGCGTGAAATGTGCTATCTTGGGTCATGAATTTGTCAGGGGC1360.10992386156059553No Hit
ACATCCGGGTACAGGCATGAGCCACCATGCCCAGCTATGGAATTTTTTATGT1360.10992386156059553No Hit
TACATAGGGATTGGAAAAAAGACAACATTGATGTTATTTGTGCTCTTTGTTT1350.10911559787264997No Hit
GCTATCGGGAACGCTTTATAATTGGCATAGACATAAAGGATAAAAGGAAAAT1350.10911559787264997No Hit
TAGTTCGGGTCCGTACATTGCTATTTTGCCACACTGCAACACCTTACAGATG1340.10830733418470441No Hit
CCACCGGGGATACAGCTGTTGACTTTCTATTGTCTGAGTCCTCCTCGGCAAG1340.10830733418470441No Hit
CACGTTGGGATCTGAAGTTGGGCAAGACAATTTAGCTTGGAAAAATAATAGT1320.10669080680881331No Hit
GTTGGTGGGTGTGGAGGGTAATCCTGCTCTTAATCGTGTGAGATGGACCCAT1320.10669080680881331No Hit
CAACTAGGGCTTCAGGAAACAGAAAAAAAGAGCACAGTTACCTAAAGTAAGC1320.10669080680881331No Hit
GGCTTTGGGGGCAGCCCGAAATCATCCTGCCCTCCAAGGCACCGCCCTGATG1320.10669080680881331No Hit
TGACACGGGCGGCCACCATGTCGCAAGAATGATGATGACAGCAGCTTTGTAG1320.10669080680881331No Hit
TGTCGCGGGGCAGTCCGGCCCTTGGAAAAGAAATCAGTGTTCATTTAAAAAT1310.10588254312086776No Hit
GTTTTGGGGGTCTGGGAAACCACTCTGTCTTGAAGATTTTTTTCCCCAAAGG1310.10588254312086776No Hit
GTATTGGGGGATCTGGTGCTAAGCCAGTGATGTCCAACAGAAATGCAATGCG1310.10588254312086776No Hit
GTCAGGGGGATCAAAAAGATATGGCAATTATAAACACACATCTAATAACAGA1300.10507427943292219No Hit
TTCTAGGGATAGTTGGAAAATAATAGAATGGCTTTAGTAGAAAAATAAATCT1300.10507427943292219No Hit
GTTGAAGGGGGTTGGGAGGGAGGCCCTCCTCCTTACTTTTCGGAATAGTTTC1300.10507427943292219No Hit
CGTATTGGGAGGACTCAGAGGCCGCCATCAACCGCCAGATCAACCTGGAGCT1300.10507427943292219No Hit
CTTTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1300.10507427943292219No Hit
TTGAGGAGGGCTGACTATATGGAATAAAACTAGAAACCAACAGTAGATGAAC1300.10507427943292219No Hit
CTGAAAGGGCTATCTGATGTCTTGTTAAACCCTCAACCGAGGAACTTTGTTC1300.10507427943292219No Hit
TAAAGTGGGCCTGGTATGCTACCATATACTAGATTTGTCTCAGCATCAGATC1290.10426601574497665No Hit
GCAGGTAGTCGATTGAGTCCACCAAAATCTTAAGCTAAATTTTATGTTGTTT1290.10426601574497665No Hit
GGAAATGGGGATACAGCAAAACTTGATTCTCCATTACCAGGCTGTACACAAA1290.10426601574497665No Hit
GATCGTGGGGTAGAGCTCATGCTCTGGAATCCAAGAGTTGTAAGAATTTCCC1290.10426601574497665No Hit
AACCACGGGGGAAGCTGCCGTGGGACTGGTGGGAGTTTCTTTATTATATTAA1290.10426601574497665No Hit
GTAATAGGGATGAGAATTGACTGCCAACTAAACCAACTGTTAAGTTATCTAG1280.10345775205703109No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCCTGTCTCTTATAC1280.10345775205703109No Hit
AACTCTGGGGATAATAAAAAGAAAGAAATTAAGTCTCGATGTAAATCATTGT1270.10264948836908552No Hit
GCGATGGGATTGATGTGTGGCTACTCAAAGTCATATCAGTGATTTACTGTAG1270.10264948836908552No Hit
CAAACAGGGAACTTATGGATGAAAGTATTCATCACAATATTATTTATAATAA1270.10264948836908552No Hit
GGTCCCGGGACCTGATATTTGACCTCAAGTGAAATAATTGGAGTGATTCAGC1270.10264948836908552No Hit
ATCAGTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1260.10184122468113997No Hit
ATTCTCGGGACCAAATTATTTATATATGCATAGTTCTATTTCTTGACTTTCT1260.10184122468113997No Hit
GTTAGTGGGGAGGAAGAGTCTGAGTTGTCTGTTTCTAGAAGAAACCTCTTAG1260.10184122468113997No Hit
TTTTTAGGGATACTCCAAACATGATTTTTTGAGGAGTGTCTGTGTTTTGGAT1260.10184122468113997No Hit
CTACTGTAGGGCAATCAATTTTTTGTGGAAAAGCCCTAGGGATTGAATTTAA1260.10184122468113997No Hit
ATTAACGGGGACATTGAAGAGATATATGCTGTTGCTGGAGTTGTTTTTCAGG1260.10184122468113997No Hit
ATCTGCGGGGACTTCCTTCGAACCAAGCAGACCCTTGGGTACCATGTCTACC1250.10103296099319442No Hit
TCGAATGGGAGTTTGGAAATATTATGAATAAAGATATTTCGAACACTTATGT1250.10103296099319442No Hit
TGCACTGGGACTCTTGGCAAAAGAAAGACATCACCAAATTTATTTTAAAGTT1250.10103296099319442No Hit
GGTTCTGGGTGAGAAATCCAAGGAAAGTCAAGTGACTGCTGATGACCTTGAA1250.10103296099319442No Hit
TCGCGTGGGGGGCGCTCCCCGGGGCAGGTGGCTGCATAGTCTTGGCGGAGGT1250.10103296099319442No Hit
TACACCAGGGCTATATTGATTTAAAAGTGTATTTTATTCAGTAGTTTGAGGA1240.10022469730524887No Hit
GGGGCTGGGGGGTGGAAGCTGAGATCCACCTCCCTCCTCCTGAGGGATGGCA1240.10022469730524887No Hit
AGACCAGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1240.10022469730524887No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)