FastQCFastQC Report
Sat 31 Dec 2022
SRR5849168.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849168.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124173
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGC8490.683723514773743No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGCCGTCT8090.6515103927584902RNA PCR Primer, Index 47 (95% over 22bp)
AAACGAGGGAATGGTGGTAGCAGCTGAGGTATGGTTGTTGGACGAGGCCGAT2620.21099594919990658No Hit
CACAGTGGGGCTCAGAGGAAGAAATTAGAATGAAAGTCATATCATACCTATC2060.1658975783785525No Hit
ACTTGCGGGAACAATATTGAATTGAGAAGCTTGTAAAGTAGGCAGAAGTAAA1970.1586496259251206No Hit
GGTCAGGAAAGTGATTGAATAAAAACGCTGAGGTGGATTGGCTGCTAGAGGC1950.15703896982435794No Hit
TATTACGGGCACCAATGGGGCTTCTACTAGGTGGCGTCAAGTCAACATATAT1940.15623364177397664No Hit
ATGGTAGGGAGGGGAACAGGTTAATTTATTGAAAGAAACAATTAAATATATG1920.15462298567321398No Hit
GGTCCTGGGATGGAAATTCAGTGGACGAGTAGTAACTGACTCAGCGGAATCA1910.15381765762283267No Hit
TTGAATGGGCCGGTTGGAAACGCCAAGCCAGCTGCGTCCTAATCCAAAAGCC1900.15301232957245134No Hit
TGGACCGGCGGGATCTCAGTAAATACATAAATAAAGAGAAATGGCAACAGCA1880.1514016734716887No Hit
CACGTTGGGCTAGCGGAGGGAACTTGAAATCAGGTAGTGTTAGCAATCCAAC1860.14979101737092604No Hit
TTACCCGGGGGAAAAAGAGGAGTCCAAGACAGCTTCTGAAGTTTTTGGTCTA1840.1481803612701634No Hit
TGTGATGGGGAGTAGGAATATGAAAATGACACCTTTTCCAAATATTAAATTG1800.1449590490686381No Hit
TTAAGTGGGACCTCTATAAAGCTGGGAAGGGGAATGTAGGTACCAGCTACAC1780.14334839296787547No Hit
CTTTGGGGTATACTTGGTCCACCGGGTTCTGTATATGAAGGTGGTGTGTTTT1760.14173773686711283No Hit
CTCGATGGGGACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTCGGC1710.1377110966152062No Hit
TCAATAGGGCACAAACAGAACAATACATTCAAAGAAATCTCATTTGCATAAC1690.13610044051444356No Hit
TCTCTAGGGAAAATTACAAATTGAAAGTCAGGGAAGACTTATCTAAGCAAAG1680.13529511246406223No Hit
AGCACTGGGAGGCTTAAATGAGGTAGCTTTTGATTCAAAATGGAAAAATAAT1670.13448978441368092No Hit
GAACTCGGGGGGAAGAGAACAAGAGTCTTTGCCTTGTAATCCAAAGAATTAT1670.13448978441368092No Hit
CGTAGGGGGCGGACTGCGGACTGCAGTGGCGCAATCTCGGCTCACTGCAAGC1640.13207380026253696No Hit
TAAACTTGGGATAGCATGCAACATAAATGAAGTTATAGATCCCCTGGCTGTT1640.13207380026253696No Hit
CGCTAAGGGGGTATGTGTGGTTGGGGAATTCATGTGGAGGTCAGAGTGGAAG1640.13207380026253696No Hit
TTTGTAGGGTTACCCAGCACCCCAGGTTTCCACAGCTGTAGTTGAGCCCTAC1640.13207380026253696No Hit
TAGAGCGGGAAGCTCGCTGCGATGCTGAGGCTGGACGGGGAGATGATTATGA1620.1304631441617743No Hit
TAGTGGGGGACCTGTTTGTCAGCCACATCACGCTGTTGACTTGTGTTAAGCT1620.1304631441617743No Hit
GACAACGGGAGTCTGCAGCCGCGACGTCTGATGTTAGGGACTAGAACTGAGA1610.129657816111393No Hit
ACACAGGGGGAAACCTGGTAGTCTGATAATAGTCTGAAACCTGATAATGGTA1610.129657816111393No Hit
TGTTTGGGGAAAGTGTTGGGATTGTAAGCATAGGCCACTGTGCCCAGCAAGA1610.129657816111393No Hit
CAGAAAATAATTAAGAAGAATTTATGTCGAAACTTCATGCTTCATCTAGTCA1600.12885248806101166No Hit
ATTTGAGGGGATGGGCACAAATGGAAGGTGCGCACTTGCCCCTACTATTGCC1600.12885248806101166No Hit
TGCATCGGGAGATGTGTGCCAGAATATTGTAAATTTTAACATAACTTTTACT1580.12724183196024902No Hit
GTCTTTGGGACCAACCCAATGAGTATCTGAAAGCTTTGGTAGCTGTGGGGGA1580.12724183196024902No Hit
GCTGTGGGGTACCATAAGCAATCCCTTGCCTTAACTCATCACCCTTTTTCAC1570.1264365039098677No Hit
ACTAGTGGGCAGAGCCAGAAAGGTCTGAGAAGTGGACTGCATAGTGCAGACC1570.1264365039098677No Hit
TGGAATGGGCAGAAGAATGGGCCTTTCTGCCATAATTCTTCACTAGTCAAGA1570.1264365039098677No Hit
TATTTAGGGTGACCGACCAACACAGTACCAAGAGGATGAAGAGAAGATGTTT1570.1264365039098677No Hit
GATAATGGGTGGCTGAGACTGCTAAGAGTGAACCTAAACACTTACAAGTTGC1550.12482584780910504No Hit
AACAGTGGGATGTTTTAAAAATAATATTCTAGCTATTATGCAGAGGATGGAT1540.12402051975872372No Hit
TTTACTGGGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCT1540.12402051975872372No Hit
GACATTGGGCTGAGTGAAATATTTGCTTTCATTCTTTCCCTTTGCCCTCTTG1540.12402051975872372No Hit
TCACTAGGGTCTTCCTGCTCATGATGCTTGGTGTTCGTGGCCTCCTCCTGGT1530.12321519170834239No Hit
TAACGAGGGGACAAATAATGATGACATTATTTGTCATCTTTGTTTGTGAGAT1510.12160453560757975No Hit
GATGTTGGGATCACCATGGGGATGGTTCTTCTAAATGAAGCAGCAACTTCGA1510.12160453560757975No Hit
ATACTGGGGGGCCCGAAGCCCGGGGACGAACCGACGGACCGACCGCCTGGCG1510.12160453560757975No Hit
TGGAAAGGGATCACAAAGGCAGCCCTCAAATTATGCAAGCTTGGAAATCACT1510.12160453560757975No Hit
TTCTAGGGATAGTTGGAAAATAATAGAATGGCTTTAGTAGAAAAATAAATCT1500.12079920755719842No Hit
CTCACCGGGATTCTCTCTCATGATTCTTGGAGGCTGGAAAGTCCAAGATCAA1500.12079920755719842No Hit
CTGCCAGGGGATTATGGAGAATTGGGGCACCCACAGGAATGAAGAGAGAAAG1500.12079920755719842No Hit
TTCTGCGGGAGTGACAATACAGAAATAAGGTGTTATTTCTGTTCAGATCTCC1500.12079920755719842No Hit
GGCTTTGGGGGCAGCCCGAAATCATCCTGCCCTCCAAGGCACCGCCCTGATG1500.12079920755719842No Hit
GATACTGGGGAATTCGAATGGACAGTTACACCAGTTACTGCAATGCTGTGTC1500.12079920755719842No Hit
TGTAGGGGGATATTGGTGAAATGGGAAAAGACTACATTTATGCCGTAACACC1480.11918855145643578No Hit
TCTTAGACAGGGCTTTCTCCCTCTGAATACACCTGTGGAGAAAATAGAGAGA1470.11838322340605445No Hit
CGCGACGGGATAATCAGAAAAGAAAATCCAGTTAAATTTATGAAGTGAGATT1470.11838322340605445No Hit
AACGCGGGGATATTTTAACAATTCACAACATACATTTTGGTGTTTCTGAAAG1460.11757789535567313No Hit
ATTCGCGGGTAAACCAAAATTAGCAATGAATCTGGCAGGCTTTTTCCAAAAG1460.11757789535567313No Hit
CAAGACGGAAAGGAGAAAAAAAATACAGAAATTGCAACTGGATCTGACATTT1450.11677256730529181No Hit
ATCACGGGCATAAAATATTTAAGTATTTGAAAAAGAAACAAAATGTCTTCAT1450.11677256730529181No Hit
TTGTTAGGGGATCTCGAGAAGGGGGAAGTGACAGGCTCAAAGCAATTGGGGC1450.11677256730529181No Hit
CTGGTTGGGGGCAGTGGTGCTGGGAGTGTCGTGGACGCCGTGCCGTTACTCG1440.11596723925491048No Hit
CTCGTTGGGGTGACTAGCAGAGTCAAGGGTTCAATAATCGTTGAATGAATTA1440.11596723925491048No Hit
ATTGCCGGGAGCTGAACAAGTTGGCCTTCCTGAACCAGTTCCACTTTGGTGT1440.11596723925491048No Hit
AATGAGGGGACACACATTAAATCTTAAAATTTTCCTGAGGTTTCTGGATATA1430.11516191120452916No Hit
CTGCATGGGATGCATCATTCTCTAGAGTTGAAAGGATTGACATTTCTCAGTA1430.11516191120452916No Hit
TTAGCTGGGTCTATGCGAGGGGTGATGTGAAAAATCAATTTATTATCCTTGG1420.11435658315414785No Hit
GTAATAGGGATGAGAATTGACTGCCAACTAAACCAACTGTTAAGTTATCTAG1420.11435658315414785No Hit
GACCGGGATCAATCTTGAGCATGAAACCACCTGAGGCCTTAGCCATGTCTCT1420.11435658315414785No Hit
TCTAAAGGGGAAGGGAGGATACCTATCAAAATGCCAAAGCTGCAGGGACACG1420.11435658315414785No Hit
ATGTAGGGGAATTTAGAATAACAACGCATCCAGGACCCTTTGGGTTACTTAA1410.11355125510376651No Hit
CGTATTGGGAGGACTCAGAGGCCGCCATCAACCGCCAGATCAACCTGGAGCT1410.11355125510376651No Hit
ATTCAGGGGGCGGCGCCTACCGCCCTGGCCGTGTCTGTCTGGTGTGTGCTGT1410.11355125510376651No Hit
AACTCTGGGGATAATAAAAAGAAAGAAATTAAGTCTCGATGTAAATCATTGT1400.1127459270533852No Hit
CTGTCGGGGTACTCTTAACCTCCCAAAAAGCAGTATCTAAGTCACATACATG1400.1127459270533852No Hit
TTGCAGGGAGACTTGAACATCTATGGATTTTGGTATTTGCAAGGGGTGAATG1400.1127459270533852No Hit
ATACTTGGGTGTCCCATCAAAATGGGCCACGTAGCATCTACTTACATCTTTG1400.1127459270533852No Hit
CACTCTAGGGGAATGAGGAAAAATATTTACAAACTATGTATCTGACAAAGAT1400.1127459270533852No Hit
GTTGGTGGGTGTGGAGGGTAATCCTGCTCTTAATCGTGTGAGATGGACCCAT1390.11194059900300388No Hit
AGTAGCGGGAGTCCACCAGCCAAGCAGCATACTTGGGTTGGTACTTTCTCTC1390.11194059900300388No Hit
TTCACCGGGAATAACCAAATGCTAAAAGAACTGGCATAGAAGTAAATGGGCT1390.11194059900300388No Hit
CTGTGCTGGGAGTGTGAATCTTATTCTGATGATTTTTTGTCCTTAGCATAGT1390.11194059900300388No Hit
AAAAAGGGGGATATGCCAGACACTATGAGAATACATTGAAAGGTTTTTATCT1390.11194059900300388No Hit
TTGCGTGGGGCCTGTTAGACTGGTGAGAGAACTCTAGGTACTTTGTTGGGAA1390.11194059900300388No Hit
TAAGTGGGTCATAAAGACTAGTTCTTATCAACCTTGAATGACTACAGATTAT1380.11113527095262254No Hit
ACACTAGGGGTAGTGGTCTGTGGCTACAGGAAGCTGGTTCTGTCTGCTTCCA1380.11113527095262254No Hit
CTGAAAGGGCTATCTGATGTCTTGTTAAACCCTCAACCGAGGAACTTTGTTC1380.11113527095262254No Hit
GAGTTGGGGGCCACTTCTTAAAACAGAACAAAACAAAAAAAGAAAATTGTGC1380.11113527095262254No Hit
AGATAGGGGAATTAGCATTTCTCTGAGGATTAGTGATGTTGAACATCTTTTT1370.11032994290224123No Hit
AAGCTGGGTCACCAAAATGTCTCCATTTGAATTGTATTGATAATGTGGGACA1370.11032994290224123No Hit
CACGTTGGGATCTGAAGTTGGGCAAGACAATTTAGCTTGGAAAAATAATAGT1360.10952461485185991No Hit
AAGCCTGGGCGACAGAGCGAGACTCAATCTTGAAACCTGGATTGAGAATGGA1360.10952461485185991No Hit
TGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG1360.10952461485185991No Hit
ACCCGTGGGCTAATGATAATAACTAATATTTCTCAGTGTTTGCCTTGTTCCA1360.10952461485185991No Hit
TTGAGGAGGGCTGACTATATGGAATAAAACTAGAAACCAACAGTAGATGAAC1360.10952461485185991No Hit
AAGCTGGGCTCTCTTTAAATCTGTTCAGTTAGTCTCATGTATTCTCAGGGGA1360.10952461485185991No Hit
GAGGCAGGAGAATCGCTTACACCTGGGAGTGGAGGAGCCAAGATCATGCCAT1360.10952461485185991No Hit
GTCAGGGGGATCAAAAAGATATGGCAATTATAAACACACATCTAATAACAGA1350.10871928680147858No Hit
GCCGTTTTCTATTTCTCCCTGGGCTGCGGAAAGCCAGAAGATTTTATCTAGC1350.10871928680147858No Hit
TACAGTGGGGGAGTGATGGGGATTTAGAAGAAAAGGATGGCTTTCTGCCAGA1350.10871928680147858No Hit
TATAGTGGGATCCTTAGAAATTATAGTTTTTAATATCTTAATATGCATCTCT1350.10871928680147858No Hit
CAGTGGGGATATTAGATTTACTGCCGTATCCCTCCATCTTACCTAAATGAGA1340.10791395875109726No Hit
TAGTTCGGGTCCGTACATTGCTATTTTGCCACACTGCAACACCTTACAGATG1340.10791395875109726No Hit
TACTCAGGGAAAAGGGAAGCAGAGCATAAAAGTTTGGAAAATTTGCAATGTC1340.10791395875109726No Hit
TGCACTGGGACTCTTGGCAAAAGAAAGACATCACCAAATTTATTTTAAAGTT1340.10791395875109726No Hit
CCAAAAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1340.10791395875109726No Hit
GCTGATGGGAGTTCAGATCTGAGTCTGAATCAAGGACACTTGAATCTGACTG1340.10791395875109726No Hit
CGATTAGGGCACTGCGAAAATTCATTCCGCTGTAATGTTTTCACAATATTTA1340.10791395875109726No Hit
CCATCCACTTCCCATTTCCTCTGCCCTTTTCCACCTCCCCCTTCCATCCCAC1340.10791395875109726No Hit
AATTTGGGGAAGAAAGCTCCATTTTTATCATAAACATTTCAACATAACCAAC1340.10791395875109726No Hit
CAATCAGGGAGAGGAGGGATGAACAGCAGCTTATGTTAAGACTCAGCGAAAA1330.10710863070071594No Hit
AAGCATGGGCCACCTATCTATTCACATGGACCAGCAAACGGGACTTCTCTCT1330.10710863070071594No Hit
GTCAAACGGGTCTTCTATCGGGGGGGCTGACTTGTGTTGTGATTATTCCAAT1330.10710863070071594No Hit
CACTAGGGGATCTTGCTCAGGGATAATGACCCAGGAATAGCTAATGACACAG1320.10630330265033461No Hit
CTTCCAAGGGAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATA1320.10630330265033461No Hit
AGAATTGGGTCTCTTCTAAGTTTCTGTCCCTCTGCTTCCTTACTTTTTTTCC1320.10630330265033461No Hit
ATATGGGGGAAATTTCTTGCAGAATCAGCTACACTTAATTATGTTGCTGATA1320.10630330265033461No Hit
TCGCGTGGGGGGCGCTCCCCGGGGCAGGTGGCTGCATAGTCTTGGCGGAGGT1320.10630330265033461No Hit
CAACTAGGGCTTCAGGAAACAGAAAAAAAGAGCACAGTTACCTAAAGTAAGC1320.10630330265033461No Hit
ATTACTGGGGACTTATAGGTGATAGTGATAATTCCTGATTCCAAGAATGCCA1310.10549797459995329No Hit
ATGTTGGGGGATGAGAAACCAGATGATGCTTTCTATTATGAGTCTATTCTAA1310.10549797459995329No Hit
TATCTACGGGAAATTATGGCATTTAGATCCAGATACCGAATATGAGATCCGA1310.10549797459995329No Hit
ATTCTCGGGACCAAATTATTTATATATGCATAGTTCTATTTCTTGACTTTCT1310.10549797459995329No Hit
ATGGCTGGGATGGAGTCCTGTGCACAAATCTGAGAAATTTTGGAGAGAGAAT1300.10469264654957197No Hit
CTCATCTCGGGAAGCAGGGAGAATAAAGATAATTGTATTTTATGTTTTGCAC1300.10469264654957197No Hit
TGTCGCGGGGCAGTCCGGCCCTTGGAAAAGAAATCAGTGTTCATTTAAAAAT1300.10469264654957197No Hit
TGCCAGGGGTTATCCAGCTAGACAATATTTTATGCATATTTACCGTGATGTC1300.10469264654957197No Hit
TTACGCGGGGAGATACTGCGAGTATGGCGGCGTCAAAGGTGAAGCAGGACAT1300.10469264654957197No Hit
TAGCCAGGGAAGTGACACTACAGAAAAACACAAAAAGGTGATGGGTTGTGTT1290.10388731849919064No Hit
CTCTAAGGGAACTATATACTTTCTACAATAAATGTATTTCAAATGTTATTAT1290.10388731849919064No Hit
AGCCACGGGGTTCCTACACTTTTGATGTCAAAGCCACTGATGGAGGACAACC1280.10308199044880932No Hit
GGTTCTGGGTGAGAAATCCAAGGAAAGTCAAGTGACTGCTGATGACCTTGAA1280.10308199044880932No Hit
GGTATTGGGAAGGCATATACAAAATAAACAGAAAAATGACACAAGACCCCTT1280.10308199044880932No Hit
GATTGAGGGTATTCTGAGACAATAGGCAAATGAATTTTTATATAAAGAAAGT1270.102276662398428No Hit
TCTCACGGGAGGAGACTGAATTAGACCCTTAAGGTATAGTGTGTGTTGCAAA1270.102276662398428No Hit
ACATCCGGGTACAGGCATGAGCCACCATGCCCAGCTATGGAATTTTTTATGT1270.102276662398428No Hit
AGTTGAGGGATCTGGATCATTAAAGTATGTCCTTGTTTAGAAACTTTGGCAG1260.10147133434804667No Hit
CTACTGTAGGGCAATCAATTTTTTGTGGAAAAGCCCTAGGGATTGAATTTAA1260.10147133434804667No Hit
GTATTGGGGGATCTGGTGCTAAGCCAGTGATGTCCAACAGAAATGCAATGCG1260.10147133434804667No Hit
GAGATGGGGCTTGGGAGGTGAGGCTTCCCAACCACGGAAGACTACTTTAAAT1250.10066600629766535No Hit
TGATGCGGGTGTGGCTCTCGGGCCTAAACTATTATAACATTTAAAAACCAAA1250.10066600629766535No Hit
CAGCGTGGGAGAGCTCTGCCCTGAGGATATAAACACATTTAAATTACTGTCA1250.10066600629766535No Hit
TTCATCGGGGTCCCCGATGACAGTGGCTTCCCACTGCAGAGACATTTTCACA1250.10066600629766535No Hit
ACTATTGGGGCACAAGGCAATTGCCTGATGTAAAAGAGATAAAGAAATGTTA1250.10066600629766535No Hit
AGCTTTGGGGCAAAACAAAAATCCAGTTTAACATGAACAAAAGATTTGAGCA1250.10066600629766535No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)