FastQCFastQC Report
Sat 31 Dec 2022
SRR5849164.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849164.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135261
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGC7970.5892311900695693No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGCCGTCT7230.5345221460731474RNA PCR Primer, Index 47 (95% over 22bp)
TTCCATGGGTAATTGTGAGTCAATTAAACCTCTTTTCTTCATAAATTACCCA1880.13899054420712548No Hit
CAAAATGGGAAGTTATGAAAATAGTTAAGTGGAAATTTGGGACTTGAAATGT1800.13307605296426908No Hit
GATGTGGGGAGTTGCAAACATGGAATATATTGATCCAACTGTATAAATAATC1790.13233674155891204No Hit
GGCTTTGGGGATGGGAGGGGCTCCCAGGCCGGCAGCCCTTGCCACCTCCCTC1720.12716156172141269No Hit
ACTGAAAGGGGTGTTTGGTTTTGGAAATATAACTTTTGTCAAAGAAATACTC1700.12568293891069857No Hit
AGCTCGGGGTTTGCAACAAAGCTTACCTGCCCGAGCAAGTACTTGCATGCCT1700.12568293891069857No Hit
GAAAAAGGGATCTACTTCGACCGCAAAAGACGAAGTGACCCCAACTTCAAGA1610.11902913626248511No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG1600.11828982485712806No Hit
TCTCGTGGGGTGATTTATAAAAGTAATAAGTGATTGATAAATTCAGTATGAT1600.11828982485712806No Hit
ACCTTGGGGATAACATTTGTGACTTTTCACAACTTCCTATCTTTCTAAAGGT1580.11681120204641397No Hit
TAGCATGGGATGGGAACTCTCTACTCTTCATTCCATTTTGTTGTGAATCTAA1570.11607189064105693No Hit
ACATTGGGAGCTCTAGAGAAAAACAGAACACCTGATTCCAGTAGTAGTTAAG1570.11607189064105693No Hit
AGTACAGTGAAATGCTGGTATTTGCAGCCTGTCATCATATTAGTACAATGAT1560.11533257923569988No Hit
TCCCGCGGGGATCTACTTGCGGGGGCCAACCATGGTGAACTTGGGTCTGTCC1550.11459326783034281No Hit
AAAACTGGGATGAAGACCAAGTGTCTCTGAAAAGTGTTTCCTTAGTGTCTGG1550.11459326783034281No Hit
CGACTTGGGCAGCCATCGAAGGACACTTGGGTTGTTTCCACCTTTTGGCTAC1530.11311464501962872No Hit
CAATCTGGGATAGGCATGAGTCACCACAATTGGCCTGCTATGTCTTTTGTTG1530.11311464501962872No Hit
AATCATGGGAGCCTTAAGAAAGAACAAAATTGAGAGCATTGAAATTCCTGAT1510.11163602220891462No Hit
CGGTGCGGGCAATTGATGAGGGCTTAAACAAAAGCTTTGGCAGTATTCAGTT1500.11089671080355756No Hit
TCTTTTGGGATAAATGGCTGTGGTGTTGGGGTGGGCATGGAGGGGCAGCGGC1500.11089671080355756No Hit
TGCTGAGGGGGGCAGAATGTTACCAATACTTTTATTAAAGATATTTGATTAA1480.10941808799284347No Hit
TAGATGGGACATGATGCTCTTAAAGGAATTTTATGCTGCCTATCCAAATTCA1480.10941808799284347No Hit
TCGCTCGGGTATAAGATTCACTCTAGACTAATTATGTAGTTAATTACTCTAG1470.1086787765874864No Hit
ACGCCTGGGAACCTTTCTTCATTTGTTCATGTTCTACTTGATGTGGTTATTG1460.10793946518212935No Hit
TTCGATGGGGAGTTTATCTGAATAAATATCATTAATGCTAATCTCTGGATTA1460.10793946518212935No Hit
AAATGTGGGCAATTCTTTTGGATAAATGCCTAGCAGTAAGATTCAGCATTCA1450.1072001537767723No Hit
GCTGCTGGGGTATCCAATAAGGTGTTGGTATCCAGAATATTTAAGAAACTCA1450.1072001537767723No Hit
TTTAAAGGGATACTATTAAAAATCAGAAGGGAGAACTAGGAGGGAAAAAAAT1450.1072001537767723No Hit
GGCGCAGGGGGACACTGTTGACCCAAAATATTCCAAATAAACAATGAGTACA1450.1072001537767723No Hit
AATGCAGGGGTTTCCATTGCGCTCTACAATGACATTTCTCACATGAAGATCA1440.10646084237141526No Hit
TATACCGGGAATGCAGTTCGCTTGTACATAAATGTAACGTCATATTGTTTAC1440.10646084237141526No Hit
CGTACAGGGTACACAGTGATTCTGAGGTTTATTTCAATTATTAGAACTGAAA1430.10572153096605821No Hit
AAAACCGGGCAAGAAACACAGGTTAAATACAAAGATGCAAACAGGTTAAAAT1430.10572153096605821No Hit
TGTACGGGATACCCACGGTAGGATTCTGCATTCCAGCATTAAATCTGCTTCA1430.10572153096605821No Hit
ACCCTTGGGGGTCCAGCTTCTTCCAGGTCTGCGCGTCTGTTGTTCCCAGCGC1410.10424290815534412No Hit
TACTTAGGGCATTTTGAAAAGACTTTGTTCTTTTCATTTTTATTTGGGAGTC1410.10424290815534412No Hit
TGCTATGGGAGATTGACCAGGTACGGTTCTATTGAATTAGGCAGGTGGAGAA1400.10350359674998708No Hit
TTGGCGCGGGATTGGCAATAGCATCATCATTGTTTGTGGCATTCCAGTTAAG1390.10276428534463No Hit
TAAGTTGGGAAAGTAACTGGTATCAGATTCTTGCTTTAATAACACCTTTCCA1380.10202497393927296No Hit
TTGGGTGGGCTCCATATGTCAGAGCCTCTCTGGGATGAAGTTCAAGCCAGAA1380.10202497393927296No Hit
AAAGAGGGTCAACTCAGTGAAGAAAAATTAACATTTTTTTGTCTGTACTTAG1380.10202497393927296No Hit
TAAAGAGGGAGGTTATCTAGATGTATGATTTACATGTATCCTACTTGTATCT1380.10202497393927296No Hit
AGTTGAGGGGGGGCATAAATAAAACTTTCATAGTACACATGAGGTGGATATT1370.10128566253391591No Hit
TCGTGAGGGTAGTTCAGAAACCAGTTTTCAAAACTTCCTTCATGTAGTAAAT1370.10128566253391591No Hit
GTTTGAGGGAACTTTTTTATTTTTGTACAGTTTCTAAGTGATTTTTGCTAGT1370.10128566253391591No Hit
TCTTTGGGGAGCCAAGCAGCCCTTCAAGTTCTTTTATTTCTGGGTAACAGAA1370.10128566253391591No Hit
CTGCCAGGGCCGTGGAAAAACATTACTTCAGGTCTTCATTAGTATATTACAG1360.10054635112855886No Hit
ATCTACGGGTATTGAATTATGTACAACATTTTCATTTTTTATGCTTCTTGAG1360.10054635112855886No Hit
AGTCAAGGGATGTAAATTTAAATATTGCTACTTATTTTTTTTCTTTTCTGAA1360.10054635112855886No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)