FastQCFastQC Report
Sat 31 Dec 2022
SRR5849163.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849163.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133355
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGC8180.6134003224476022No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGCCGTCT6740.5054178695961906RNA PCR Primer, Index 47 (95% over 22bp)
TTCCATGGGTAATTGTGAGTCAATTAAACCTCTTTTCTTCATAAATTACCCA2160.16197367927711748No Hit
TCGTGAGGGTAGTTCAGAAACCAGTTTTCAAAACTTCCTTCATGTAGTAAAT1900.14247684751227926No Hit
GGCTTTGGGGATGGGAGGGGCTCCCAGGCCGGCAGCCCTTGCCACCTCCCTC1850.13872745678827192No Hit
TCCCGCGGGGATCTACTTGCGGGGGCCAACCATGGTGAACTTGGGTCTGTCC1800.13497806606426455No Hit
GATGTGGGGAGTTGCAAACATGGAATATATTGATCCAACTGTATAAATAATC1770.13272843162986014No Hit
CAAAATGGGAAGTTATGAAAATAGTTAAGTGGAAATTTGGGACTTGAAATGT1670.12522965018184545No Hit
AGTCAAGGGATGTAAATTTAAATATTGCTACTTATTTTTTTTCTTTTCTGAA1600.11998050316823516No Hit
ACTGAAAGGGGTGTTTGGTTTTGGAAATATAACTTTTGTCAAAGAAATACTC1590.11923062502343369No Hit
TCTTTGGGGAGCCAAGCAGCCCTTCAAGTTCTTTTATTTCTGGGTAACAGAA1580.11848074687863222No Hit
TACTGGGGGTGGATACTGGGCCCGGGGACCACTTTCATACTCACGTCCTCAT1560.11698099058902929No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG1560.11698099058902929No Hit
AATGCAGGGGTTTCCATTGCGCTCTACAATGACATTTCTCACATGAAGATCA1550.11623111244422782No Hit
CGGTGCGGGCAATTGATGAGGGCTTAAACAAAAGCTTTGGCAGTATTCAGTT1550.11623111244422782No Hit
TTGGCGCGGGATTGGCAATAGCATCATCATTGTTTGTGGCATTCCAGTTAAG1550.11623111244422782No Hit
TTTATAGGGAGTTATGTATCTTAGATTTCACTATAGAGACCCTATTGGCTTT1530.11473135615462488No Hit
TAAAGAGGGAGGTTATCTAGATGTATGATTTACATGTATCCTACTTGTATCT1530.11473135615462488No Hit
TTTTATGGGATTTCAAGAGAAAGAAAAATAGCCTTTTCTTTATAGCCAGAGT1520.11398147800982342No Hit
ATTTATGGGATGGTTTTTACTGATCATTTCTAGTCTCACATTCAGGTGGCTC1520.11398147800982342No Hit
TCTCGTGGGGTGATTTATAAAAGTAATAAGTGATTGATAAATTCAGTATGAT1520.11398147800982342No Hit
TTGGGTGGGCTCCATATGTCAGAGCCTCTCTGGGATGAAGTTCAAGCCAGAA1510.11323159986502195No Hit
TAGATGGGACATGATGCTCTTAAAGGAATTTTATGCTGCCTATCCAAATTCA1510.11323159986502195No Hit
AATAGGACATTTTACAAAATGGCAAGTATGGAAAACCATGGATTCTGAAAGT1500.11248172172022046No Hit
GGTTATGGGAATGCCATGAAATAATCTTTGTCTTCTGTTCAGTTGTGATTAT1490.11173184357541899No Hit
GCTTTGGGGAAGAGCAGACCCAAAGGACTTCAATTGTTGGGAATCGTTAGGA1480.11098196543061752No Hit
GGTCAGTCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC1470.11023208728581604No Hit
ACGCCTGGGAACCTTTCTTCATTTGTTCATGTTCTACTTGATGTGGTTATTG1460.10948220914101459No Hit
CGACTTGGGCAGCCATCGAAGGACACTTGGGTTGTTTCCACCTTTTGGCTAC1460.10948220914101459No Hit
TACTTAGGGCATTTTGAAAAGACTTTGTTCTTTTCATTTTTATTTGGGAGTC1460.10948220914101459No Hit
AAGTTATGGGAGGAGGAACTTGAAAGGAGGGTAAAAAGACTAAAATGAGGGG1450.10873233099621311No Hit
ACCTTGGGGATAACATTTGTGACTTTTCACAACTTCCTATCTTTCTAAAGGT1450.10873233099621311No Hit
GAAAAAGGGATCTACTTCGACCGCAAAAGACGAAGTGACCCCAACTTCAAGA1440.10798245285141164No Hit
AGCTCGGGGTTTGCAACAAAGCTTACCTGCCCGAGCAAGTACTTGCATGCCT1440.10798245285141164No Hit
AAAGAGGGTCAACTCAGTGAAGAAAAATTAACATTTTTTTGTCTGTACTTAG1430.10723257470661017No Hit
AAAACTGGGATGAAGACCAAGTGTCTCTGAAAAGTGTTTCCTTAGTGTCTGG1430.10723257470661017No Hit
AGTTGCGGGGCACGCGTGAGACTTCTCCGCCGCAGACGCCGCCGCGATGCGC1420.10648269656180871No Hit
CTGTAGGTGGGCAGAGGAAAAATTAGAAAATAAAGTAAATTATTTTAAAAAA1420.10648269656180871No Hit
GAACAAGGGATCATTTGTTACTCAAATTATTCTTTTTTGAAGTATCTTCCTC1420.10648269656180871No Hit
TCTTTTGGGATAAATGGCTGTGGTGTTGGGGTGGGCATGGAGGGGCAGCGGC1410.10573281841700724No Hit
GGCGCAGGGGGACACTGTTGACCCAAAATATTCCAAATAAACAATGAGTACA1410.10573281841700724No Hit
AACGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1400.10498294027220577No Hit
TAAGTTGGGAAAGTAACTGGTATCAGATTCTTGCTTTAATAACACCTTTCCA1400.10498294027220577No Hit
CATGCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1390.1042330621274043No Hit
TGATTGGGGACTCCCACCGAGGCCCTGCAACAGCACCAAGTCTATTTCTAGG1390.1042330621274043No Hit
AATCATGGGAGCCTTAAGAAAGAACAAAATTGAGAGCATTGAAATTCCTGAT1390.1042330621274043No Hit
AATACAGGGCGATCTCACAATGGGATGTATCTCAGAGAGTCTGTGATTCTGC1390.1042330621274043No Hit
CATAATGGGAAGGACGGTCTGAAGAGCCAAGAGCTATTTGATGAAATTAGAA1380.10348318398260284No Hit
ACGGGGGGCACCAGTAGTTTTTGAGAGTTCAGCTTCTTCCATGTCTATTCCA1360.1019834276929999No Hit
TAGCATGGGATGGGAACTCTCTACTCTTCATTCCATTTTGTTGTGAATCTAA1360.1019834276929999No Hit
CTAGTACGGGACCCAGGCTGGTCTCAACTCCTAGTGTCAAGAGATCCCCCCA1360.1019834276929999No Hit
GCCATGGGGAAGATTGTGACACCAGGCTTACCTTTTAAAGTTTAGTATATAG1360.1019834276929999No Hit
GATTAAGGGGAGGAACTTCATCCCACTGAAATTCCTTTGGCATTTGGGGTTT1360.1019834276929999No Hit
AAAACCGGGCAAGAAACACAGGTTAAATACAAAGATGCAAACAGGTTAAAAT1360.1019834276929999No Hit
CGCAGTGGGGAACATGTCATCATTCCCCGGATTGAACGTACTCTTGCTTATA1360.1019834276929999No Hit
CCAAAAGGGGAATTCGAGGAAGATGATGTGAATCTTCCAATTTTATCTATTT1340.10048367140339694No Hit
TCCAATGGGTATTAGTAGACAAGGTCTTTGTTCAGACGATTAGGTGCCTAAC1340.10048367140339694No Hit
TGTTGACGGGAGGAAGCTTCAGTTTTGGATTTTTAGAGCTTCTTCTAAGTAC1340.10048367140339694No Hit
GTCAATGGGTCACAGGATAGGAATTCCCATTTTTGAAAATATGATAACCTAC1340.10048367140339694No Hit
GTGTGAGGGGTGTGGAATAATGTGGTTTCAGGAGAAATATTTACCTAAATTT1340.10048367140339694No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)